Prognostic value of TMEM59L and its genomic and immunological characteristics in cancer by Chang Shi, Chang Shi, Lizhi Zhang, Dan Chen, Hong Wei, Wenjing Qi, Pengxin Zhang, Huiqi Guo, Lei Sun

“…A significant correlation was also observed between TMEM59L expression and immunophenoscore, homologous recombination deficiency, loss of heterozygosity, tumor stemness score, and neoantigens in various cancers. …”
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The bile salt deoxycholate induces Campylobacter jejuni genetic point mutations that promote increased antibiotic resistance and fitness by Prabhat K. Talukdar, Torin M. Crockett, Lisa M. Gloss, Steven Huynh, Steven A. Roberts, Kyrah L. Turner, Sebastien T. E. Lewis, Tristin L. Herup-Wheeler, Craig T. Parker, Michael E. Konkel

“…Finally, we found that the base excision repair (BER), homologous recombination repair (HRR), and nucleotide excision repair (NER) are involved in general oxidative damage repair in C. jejuni but that the BER pathway plays the primary role in the repair of the 8-oxo-dG lesion. …”
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Investigation of BRCAness associated miRNA-gene axes in breast cancer: cell-free miR-182-5p as a potential expression signature of BRCAness by Farzaneh Darbeheshti, Sepideh Kadkhoda, Mahsa Keshavarz-Fathi, Sepideh Razi, Afshin Bahramy, Yaser Mansoori, Nima Rezaei

“…Breast tumors with BRCAness have deficiencies in homologous recombination repair (HRR), like BRCA1/2-mutation carriers, and consequently could benefit from poly-(ADP)-ribose polymerase (PARP) inhibitors and DNA-damaging chemotherapy. …”
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ECPPF (E2F1, CCNA2, POLE, PPP2R1A, FBXW7) stratification: Profiling high-risk subtypes of histomorphologically low-risk and treatment-insensitive endometrioid endometrial cancer by Jesus Gonzalez-Bosquet, S. John Weroha, Jamie N. Bakkum-Gamez, Amy L. Weaver, Michaela E. McGree, Sean C. Dowdy, Abimbola O. Famuyide, Benjamin R. Kipp, Kevin C. Halling, Siddhartha Yadav, Fergus J. Couch, Karl C. Podratz

“…Expression of homologous recombination repair (HR) and cell cycle genes was significantly elevated in CCNA2-H/E2F1-H compared with CCNA2-L/E2F1-L (P<1.0E-10), suggesting that HR deficiencies may underlie the favorable PFS in MLR. …”
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Identification of DNA Damage Repair-Associated Prognostic Biomarkers for Prostate Cancer Using Transcriptomic Data Analysis by Pai-Chi Teng, Shu-Pin Huang, Chia-Hsin Liu, Ting-Yi Lin, Yi-Chun Cho, Yo-Liang Lai, Shu-Chi Wang, Hsin-Chih Yeh, Chih-Pin Chuu, Deng-Neng Chen, Wei-Chung Cheng, Chia-Yang Li

“…For example, olaparib was just approved in May 2020 to treat metastatic castration-resistant PC with homologous recombination repair-mutated genes; however, not all patients can benefit from olaparib, and the treatment response depends on patient-specific mutations. …”
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Construction and otological phenotypic characterization of a mouse model harboring orthologous human Waardenburg syndrome mutation SOX10 p.R106W by WANG Lulu, XIE Fei, ZHAO Qingyuan

“…Methods By comparing the amino acid sequences of SOX10 proteins from human, miniature pig and mouse, the mouse orthologous site corresponding to human SOX10 p.R106W mutation was identified, and the nucleic acid mutation Sox10 c.316A > T coding Sox10 p.R106W was precisely introduced into the orthologous site of mouse Sox10 coding sequence by CRISPR/Cas9-induced homologous recombination. Characteristics of otological phenotypes were assessed by auditory brainstem response test and cochlear tissue histology. mRNA sequencing was conducted on cochlear tissues, and differential expressed genes (DEGs) were accepted gene ontology (GO) analysis. …”
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Diagnostic Potential of Endometrial Cancer DNA from Pipelle, Pap-Brush, and Swab Sampling by Yinan Wang, Hui Du, Wenkui Dai, Cuijun Bao, Xi Zhang, Yan Hu, Zhiyu Xie, Xin Zhao, Changzhong Li, Wenyong Zhang, Ruifang Wu

“…TPS produced high-quality data (Q30 > 85% and mapping ratios > 99.35%) and was found to have strong consistency with whole-exome sequencing (WES) in detecting pathogenic mutations (92.11%), calculating homologous recombination deficiency (HRD) scores (r = 0.65), and assessing the microsatellite instability (MSI) status of EC (100%). …”
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Prevalence, Molecular Characteristics and Virulence Identification of Bovine Parainfluenza Virus Type 3 in China by Xiaowen Xu, Wanyue Zhao, Zhijie Xiang, Chen Wang, Mingpu Qi, Sen Zhang, Yuanchen Geng, Yuhao Zhao, Kaihui Yang, Yanan Zhang, Aizhen Guo, Yingyu Chen

“…Genetic recombination analyses provided evidence for homologous recombination of BPIV-3 of bovine origin. The virulence experiment indicated that strain Hubei-03 had the highest pathogenicity and could be used as a vaccine candidate. …”
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Secretory Expression and Properties of Laccase from Ganoderma lucidum in Aspergillus niger by Yu LIU, Tao YU, Yuxin ZHANG, Hui ZHANG, Tianqi LIU, Jie LI

“…By Agrobacterium tumefaciens-mediated transformation of Aspergillus niger TH-2, homozygous transformants of Aspergillus niger with homologous recombination at the glaA gene locus were screened. …”
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Abrogation of KLF5 sensitizes BRCA1-proficient pancreatic cancer to PARP inhibition by Zhang Zheng, Liu Yuxin, Xu Yaolin, Xu Zijin, Jia Jinbin, Jin Yun, Wang Wenquan, Liu Liang

“…In the present study, we discover that abrogation of Kruppel-like factor 5 (KLF5) significantly inhibits homologous recombination, which is the main mechanism for DNA double-stranded repair. …”
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Mechanisms of synthetic lethality between BRCA1/2 and 53BP1 deficiencies and DNA polymerase theta targeting by George E. Ronson, Katarzyna Starowicz, Elizabeth J. Anthony, Ann Liza Piberger, Lucy C. Clarke, Alexander J. Garvin, Andrew D. Beggs, Celina M. Whalley, Matthew J. Edmonds, James F. J. Beesley, Joanna R. Morris

“…Abstract A synthetic lethal relationship exists between disruption of polymerase theta (Polθ), and loss of either 53BP1 or homologous recombination (HR) proteins, including BRCA1; however, the mechanistic basis of these observations are unclear. …”
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Enhancement of Radiation Effectiveness in Cervical Cancer Cells by Combining Ionizing Radiation with Hyperthermia and Molecular Targeting Agents by Marloes IJff, Bregje van Oorschot, Arlene L. Oei, Przemek M. Krawczyk, Hans M. Rodermond, Lukas J. A. Stalpers, H. Petra Kok, Johannes Crezee, Nicolaas A. P. Franken

“…Both &alpha; and &beta; were significantly increased for HSP90-i combined with hyperthermia in HeLa cells, though not in SiHa cells. The Homologous Recombination pathway is inhibited by hyperthermia. …”
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Genetic Variants and Tumor Immune Microenvironment: Clues for Targeted Therapies in Inflammatory Breast Cancer (IBC) by Yulan Gong, Rajeswari Nagarathinam, Maria F. Arisi, Lorenzo Gerratana, Jennifer S. Winn, Michael Slifker, Jianming Pei, Kathy Q. Cai, Zachary Hasse, Elias Obeid, Julio Noriega, Christopher Sebastiano, Eric Ross, Katherine Alpaugh, Massimo Cristofanilli, Sandra V. Fernandez

“…Furthermore, 31% of IBC patients showed deficiencies in the homologous recombination repair (HRR) pathway (BRCA1, BRCA2, PALB2, RAD51C, ATM, BARD1) making them sensitive to poly (ADP-ribose) polymerase (PARP) inhibitors. …”
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The Involvement of the csy1 Gene in the Antimicrobial Resistance of Acinetobacter baumannii by Tingting Guo, Tingting Guo, Tingting Guo, Xiaoli Sun, Mengying Li, Mengying Li, Yuhang Wang, Hongmei Jiao, Guocai Li, Guocai Li, Guocai Li

“…Thus, the RecAb homologous recombination system was utilized to knock out the csy1 gene from A. baumannii AB43, which carries the Type I-Fb CRISPR-Cas system, and to observe the drug resistance changes in wild-type and csy1-deleted strains. …”
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FgLEU1 Is Involved in Leucine Biosynthesis, Sexual Reproduction, and Full Virulence in <i>Fusarium graminearum</i> by Shaohua Sun, Mingyu Wang, Chunjie Liu, Yilin Tao, Tian Wang, Yuancun Liang, Li Zhang, Jinfeng Yu

“…For functional characterization, we constructed a deletion mutant of <i>FgLEU1</i> (Δ<i>LEU1</i>) through homologous recombination. Compared with the wild-type strain PH-1, Δ<i>LEU1</i> showed slower colony growth and fewer aerial mycelia. …”
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Whole-Exome Sequencing of Rare Site Endometriosis-Associated Cancer by Sonomi Kurose, Kentaro Nakayama, Sultana Razia, Masako Ishikawa, Tomoka Ishibashi, Hitomi Yamashita, Seiya Sato, Asuka Sakiyama, Shinya Yoshioka, Misa Kobayashi, Satoru Nakayama, Yoshiro Otuski, Noriyoshi Ishikawa, Satoru Kyo

“…The patient with undifferentiated carcinoma exhibited hypermutator and homologous recombination deficiency phenotypes. The dominant mutation signatures were signature 30 (small subset of breast cancers) and 19 (pilocytic astrocytoma) in patient 1, and signature 5 (small subset of breast cancers) and 3 (breast, ovarian, and pancreatic cancers) in patient 2. …”
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Metastasis Suppressor NME1 Modulates Choice of Double-Strand Break Repair Pathways in Melanoma Cells by Enhancing Alternative NHEJ while Inhibiting NHEJ and HR by Gemma Puts, Stuart Jarrett, Mary Leonard, Nicolette Matsangos, Devin Snyder, Ying Wang, Richard Vincent, Benjamin Portney, Rachel Abbotts, Lena McLaughlin, Michal Zalzman, Feyruz Rassool, David Kaetzel

“…NME1 was detected up to 5 kb from the break site after DSB induction, suggesting a role in extending chromatin reorganization away from the repair site. shRNA-mediated silencing of NME1 expression led to increases in the homologous recombination (HR) and non-homologous end-joining (NHEJ) pathways of double-strand break repair (DSBR), and reduction in the low fidelity, alternative-NHEJ (A-NHEJ) pathway. …”
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TRF1 averts chromatin remodelling, recombination and replication dependent-break induced replication at mouse telomeres by Rosa Maria Porreca, Emilia Herrera-Moyano, Eleni Skourti, Pui Pik Law, Roser Gonzalez Franco, Alex Montoya, Peter Faull, Holger Kramer, Jean-Baptiste Vannier

“…Surprisingly, mTRF1 suppresses the accumulation of promyelocytic leukemia (PML) protein, BRCA1 and the SMC5/6 complex at telomeres, which is associated with increased Homologous Recombination (HR) and TERRA transcription. We uncovered a previously unappreciated role for mTRF1 in the suppression of telomere recombination, dependent on SMC5 and also POLD3 dependent Break Induced Replication at telomeres. …”
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Histone H3 serine-57 is a CHK1 substrate whose phosphorylation affects DNA repair by Nikolaos Parisis, Pablo D. Dans, Muhammad Jbara, Balveer Singh, Diane Schausi-Tiffoche, Diego Molina-Serrano, Isabelle Brun-Heath, Denisa Hendrychová, Suman Kumar Maity, Diana Buitrago, Rafael Lema, Thiziri Nait Achour, Simona Giunta, Michael Girardot, Nicolas Talarek, Valérie Rofidal, Katerina Danezi, Damien Coudreuse, Marie-Noëlle Prioleau, Robert Feil, Modesto Orozco, Ashraf Brik, Pei-Yun Jenny Wu, Liliana Krasinska, Daniel Fisher

“…In fission yeast, mutation of all H3 alleles to S57A abrogated DNA repair by both non-homologous end-joining and homologous recombination, while cells with phospho-mimicking S57D alleles were partly compromised for both repair pathways, presented aberrant Rad52 foci and were strongly sensitised to replication stress. …”
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Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations by François M. Seys, Christopher M. Humphreys, Claudio Tomi-Andrino, Claudio Tomi-Andrino, Claudio Tomi-Andrino, Qi Li, Thomas Millat, Sheng Yang, Nigel P. Minton

“…As such, they hold great potential for the engineering of microbes that lack effective DSB repair pathways such as homologous recombination (HR) or non-homologous end-joining (NHEJ). …”
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