Inhibition of CXorf56 promotes PARP inhibitor-induced cytotoxicity in triple-negative breast cancer by Ying Zhu, Zhixian Liu, Liang Gui, Wen Yun, Changfei Mao, Rong Deng, Yufeng Yao, Qiao Yu, Jifeng Feng, Hongxia Ma, Wei Bao

“…Abstract Poly(ADP-ribose) polymerase inhibitors (PARPis) induce DNA lesions that preferentially kill homologous recombination (HR)-deficient breast cancers induced by BRCA mutations, which exhibit a low incidence in breast cancer, thereby limiting the benefits of PARPis. …”
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Chromatin assembly factor-1 preserves genome stability in ctf4∆ cells by promoting sister chromatid cohesion by Nagham Ghaddar, Pierre Luciano, Vincent Géli, Yves Corda

“…In the absence of CTF4, CAF-1 is essential for viability in cells experiencing replication problems, in cells lacking functional S-phase checkpoint or functional spindle checkpoint, and in cells lacking DNA repair pathways involving homologous recombination. We present evidence that CAF-1 affects cohesin association to chromatin in a DNA-damage-dependent manner and is essential to maintain cohesion in the absence of CTF4. …”
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<i>PafS</i> Containing GGDEF-Domain Regulates Life Activities of <i>Pseudomonas glycinae</i> MS82 by Xianyi Chen, Shaoxuan Qu, Xin Luo, Shi-En Lu, Youzhou Liu, Huiping Li, Lijuan Hou, Jinsheng Lin, Ning Jiang, Lin Ma

“…The <i>PafS-</i>deletion mutant Δ<i>PafS</i> and reversion mutant <i>PafS</i>-comp were constructed by the method of biparental conjugation and homologous recombination. The life activities of the mutants, such as antifungal activity, biofilm formation ability, polysaccharide content, and motor behavior, were explored. …”
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Immunogenicity of live phoP gene deletion strain of Riemerella anatipestifer serotype 1 by Jian Li, Yanhao Zhang, Ying Wang, Yang Zhang, Baolan Shi, Luoxin Gan, Shuang Yu, Xiangchao Jia, Kang Yang, Zili Li

“…In this study, the phoP gene from the R. anatipestifer YM strain was knocked out using homologous recombination technology and replaced with the spectinomycin resistance gene (Spec). …”
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Mice Lacking Gpr75 are Hypophagic and Thin by Powell DR, Doree DD, DaCosta CM, Platt KA, Brommage R, Buhring L, Revelli JP, Shadoan MK

“…We wanted to confirm these findings in Gpr75 KO mice and determine whether decreased energy intake and/or increased energy expenditure contributed to the thin phenotype.Methods: Gpr75 KO mice were generated by homologous recombination. All studies compared female and male Gpr75 KO mice to their wild type (WT) littermates. …”
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Targeting branched N-glycans and fucosylation sensitizes ovarian tumors to immune checkpoint blockade by Hao Nie, Pratima Saini, Taito Miyamoto, Liping Liao, Rafal J. Zielinski, Heng Liu, Wei Zhou, Chen Wang, Brennah Murphy, Martina Towers, Tyler Yang, Yuan Qi, Toshitha Kannan, Andrew Kossenkov, Hiroaki Tateno, Daniel T. Claiborne, Nan Zhang, Mohamed Abdel-Mohsen, Rugang Zhang

“…However, it’s unclear whether homologous recombination (HR) status-dependent glycosylation can be therapeutically explored. …”
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Effects of the pyrE deletion mutant from Bacillus thuringiensis on gut microbiota and immune response of Spodoptera exigua by Dan Zhao, Han Wu, Han Wu, Yazi Li, Qian Wang, Yujie Ji, Xiaochang Guo, Wei Guo, Wei Guo

“…To elucidate the regulatory genes involved in the interaction between Bt and gut microbiota, we investigate the effects of uracil derived from Bt on gut microbiota, and host immunity using a uracil deficient Bt strain (Bt GS57△pyrE) obtained by homologous recombination. We analyze the biological characteristics of the uracil deficient strain and found that the deletion of uracil in Bt GS57 strain changed the diversity of gut bacteria in Spodoptera exigua, as investigated using Illumina HiSeq sequencing. …”
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Identification and Molecular Characterization of a Novel Large-Scale Variant (Exons 4_18 Loss) in the LDLR Gene as a Cause of Familial Hypercholesterolaemia in an Italian Family by Paola Concolino, Elisa De Paolis, Simona Moffa, Maria Elisabetta Onori, Laura Soldovieri, Claudio Ricciardi Tenore, Maria De Bonis, Claudio Rabacchi, Concetta Santonocito, Martina Rinelli, Sebastiano Calandra, Andrea Giaccari, Andrea Urbani, Angelo Minucci

“…Two Alu sequences, identified within intron 3 and exon 18, could underlie the identified rearrangement by a nonallelic homologous recombination (NAHR) mechanism. NGS proved to be an effective tool suitable for the identification of CNVs, together with small-scale alterations in the FH-related genes. …”
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RNA helicase DDX3 regulates RAD51 localization and DNA damage repair in Ewing sarcoma by Matthew E. Randolph, Marwa Afifi, Aparna Gorthi, Rachel Weil, Breelyn A. Wilky, Joshua Weinreb, Paul Ciero, Natalie ter Hoeve, Paul J. van Diest, Venu Raman, Alexander J.R. Bishop, David M. Loeb

“…We show that DDX3 interacts with several proteins involved in homologous recombination, including RAD51, RECQL1, RPA32, and XRCC2. …”
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Global analysis of double-strand break processing reveals in vivo properties of the helicase-nuclease complex AddAB by Badrinarayanan, Anjana, Le, Tung, Spille, Jan Hendrik, Cisse, Ibrahim I, Laub, Michael T

“…In bacteria, double-strand break (DSB) repair via homologous recombination is thought to be initiated through the bi-directional degradation and resection of DNA ends by a helicase-nuclease complex such as AddAB. …”
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Exploring the mutagenic consequences of inflammation and DNA damage by Kay, Jennifer Elizabeth, Ph. D. Massachusetts Institute of Technology

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Automated fluorescence intensity and gradient analysis enables detection of rare fluorescent mutant cells deep within the tissue of RaDR mice by Rajapakse, Jagath, Wadduwage, Dushan Nawoda, Kay, Jennifer Elizabeth, Singh, Vijay Raj, Kiraly, Orsolya, Sukup Jackson, Michelle Rae, Engelward, Bevin P, So, Peter T. C.

“…Homologous recombination (HR) events are key drivers of cancer-promoting mutations, and the ability to visualize these events in situ provides important information regarding mutant cell type, location, and clonal expansion. …”
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CDK12 regulates DNA repair genes by suppressing intronic polyadenylation by Dubbury, Sara J., Boutz, Paul L., Sharp, Phillip A.

“…Mutations that attenuate homologous recombination (HR)-mediated repair promote tumorigenesis and sensitize cells to chemotherapeutics that cause replication fork collapse, a phenotype known as ‘BRCAness’1. …”
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Genetic and epigenetic regulation of the cell wall glycoproteins, Flo10p and Flo11p, generate phenotypic variation in S. cerevisiae by Halme, Adrian Jones, 1971-

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Genomic signatures of sex, selection and speciation in the microbial world by Shapiro, B. Jesse (Benjamin Jesse)

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Multiplexed DNA repair assays for multiple lesions and multiple doses via transcription inhibition and transcriptional mutagenesis by Nagel, Zachary D., Margulies, Carrie Marie, Chaim, Isaac Alexander, McRee, Siobhan K., Mazzucato, Patrizia, Ahmad, Anwaar, Abo, Ryan, Butty, Vincent, Forget, Anthony L., Samson, Leona D.

“…FM-HCR simultaneously measures repair capacity in any four of the following pathways: nucleotide excision repair, mismatch repair, base excision repair, nonhomologous end joining, homologous recombination, and methylguanine methyltransferase. …”
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Automated fluorescence intensity and gradient analysis enables detection of rare fluorescent mutant cells deep within the tissue of RaDR mice by Wadduwage, Dushan N., Kay, Jennifer, Singh, Vijay Raj, Kiraly, Orsolya, Sukup-Jackson, Michelle R., Rajapakse, Jagath, Engelward, Bevin P., So, Peter T. C.

“…Homologous recombination (HR) events are key drivers of cancer-promoting mutations, and the ability to visualize these events in situ provides important information regarding mutant cell type, location, and clonal expansion. …”
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Functional analysis of clinical BARD1 germline variants by Toh, Ming Ren, Chong, Siao Ting, Chan, Sock Hoai, Low, Chen Ee, Nur Diana Binte Ishak, Lim, Jing Quan, Courtney, Eliza, Ngeow, Joanne

“…As BARD1 encompasses multiple functional domains, including those of apoptosis and homologous recombination repair, we hypothesized that the function being impaired would correspond with the domain where the variant was located. …”
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Identification of novel genes in the carotenogenic and oleaginous yeast Rhodotorula toruloides through genome-wide insertional mutagenesis by Liu, Yanbin, Koh, Chong Mei John, Yap, Sihui Amy, Du, Minge, Hlaing, Mya Myintzu, Ji, Lianghui

“…Deletion of CAR1 by homologous recombination led to a phenotype similar to RAM5 and it could be genetically complemented by re-introduction of the wild-type CAR1 genome sequence. …”
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Model-guided metabolic gene knockouts strategies for enhanced succinic acid production in Escherichia Coli K-12 strain by Mienda, Bashir Sajo

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