Establishment of Human Lung Adenocarcinoma Radioresistant Cell Lines
and the Mechanism of Radioresistance by Jingjing ZHANG, Shenglin MA, Qiong WU

“…Relative expression levels of p-DNA-PKcs (Ser2056), 53BP1 in the nonhomologous end-joining (NHEJ) repair pathway and p-ATM (Ser1981), RAD51 in the homologous recombination (HR) repair pathway were higher than those in H1975 and H1299. …”
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A novel RofA-family transcriptional regulator, GadR, controls the development of acid resistance in Listeria monocytogenes by Jialun Wu, Olivia McAuliffe, Conor P. O'Byrne

“…When this open reading frame was restored through homologous recombination, an acid-resistant phenotype was restored. …”
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Generation and Characterization of Recombinant Pseudorabies Virus Delivering African Swine Fever Virus <i>CD2v</i> and <i>p54</i> by Jianhui Wei, Chuancheng Liu, Xinyan He, Bilal Abbas, Qi Chen, Zhaolong Li, Zhihua Feng

“…In this study, we engineered a pseudorabies recombinant virus (PRV) expressing ASFV CD2v and p54 proteins (PRV-∆TK-(CD2v)-∆gE-(p54)) using CRISPR/Cas9 and homologous recombination technology. PRV-∆TK-(CD2v)-∆gE-(p54) effectively delivers <i>CD2v</i> and <i>p54</i>, and it exhibits reduced virulence. …”
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<i>BrDMC1</i>, a Recombinase Gene, Is Involved in Seed Germination in <i>Brassica rapa</i> under Salt Stress by Xulin Wang, Zhengqing Xie, Zhaoran Tian, Shuaipeng Wang, Gongyao Shi, Weiwei Chen, Gangqiang Cao, Baoming Tian, Xiaochun Wei, Luyue Zhang, Fang Wei

“…Recombinases are in part responsible for homologous recombination and genome integrity during DNA repair. …”
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MAGI1 Prevents Senescence and Promotes the DNA Damage Response in ER⁺ Breast Cancer by Janine Wörthmüller, Simona Disler, Sylvain Pradervand, François Richard, Lisa Haerri, Gustavo A. Ruiz Buendía, Nadine Fournier, Christine Desmedt, Curzio Rüegg

“…An analysis of human BC patients’ transcriptomic data revealed that patients with low MAGI1 levels had a higher tumor mutational burden and homologous recombination deficiency. Moreover, MAGI1 expression levels negatively correlated with PI3K/AKT and MAPK signaling, which confirmed our in vitro observations. …”
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Case report: Response to platinum agents and poly (adenosine diphosphate-ribose) polymerase inhibitor in a patient with BRCA1 c.5096G>A (R1699Q) intermediate-risk variant by Ayumi Saito, Maki Tanioka, Makoto Hirata, Tomoko Watanabe, Yoko Odaka, Tatsunori Shimoi, Kazuki Sudo, Emi Noguchi, Mitsuya Ishikawa, Kan Yonemori

“…This patient had no other gene mutations which could cause homologous recombination deficiency. Conclusion: This is the first report of the clinical outcome of PARP inhibitor and platinum-containing chemotherapy in a patient with a BRCA1 R1699Q variant. …”
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Hyperthermia as a Potential Cornerstone of Effective Multimodality Treatment with Radiotherapy, Cisplatin and PARP Inhibitor in <i>IDH1</i>-Mutated Cancer Cells by Mohammed Khurshed, Elia Prades-Sagarra, Sarah Saleh, Peter Sminia, Johanna W. Wilmink, Remco J. Molenaar, Hans Crezee, Cornelis J. F. van Noorden

“…Intracellular accumulation of <i>D</i>-2HG has been implicated in suppressing homologous recombination and renders <i>IDH1</i>^MUT cancer cells sensitive to DNA-repair-inhibiting agents, such as poly-(adenosine 5′-diphosphate–ribose) polymerase inhibitors (PARPi). …”
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A novel transposon Tn7540 carrying blaNDM-9 and fosA3 in chromosome of a pathogenic multidrug-resistant Salmonella enterica serovar Indiana isolated from human faeces by Yao Sun, Yijia Han, Changrui Qian, Qi Zhang, Zhuocheng Yao, Weiliang Zeng, Tieli Zhou, Zhongyong Wang

“…Sequence analysis and the formed circular intermediate suggested Tn7540 might be generated through homologous recombination by IS26-bounded translocatable units (IS26-fosA-IS26-intI1-dfrA12-aadA2-sul1-ISCR1-blaNDM-9-IS26). …”
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In silico identification of prospective virulence factors associated with candidiasis in Meyerozyma guilliermondii strain SO from genome dataset by Robiatul Azilah Zainudin, Suriana Sabri, Abu Bakar Salleh, Arpah Abu, Raja Farhana Raja Khairuddin, Siti Nurbaya Oslan

“…On account of this finding, the strategy of gene knockout through CRISPR-Cas9 or homologous recombination strategies is needed to engineer the feasible novel expression host system. …”
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The History of Live Attenuated <i>Centrin</i> Gene-Deleted <i>Leishmania</i> Vaccine Candidates by Greta Volpedo, Parna Bhattacharya, Sreenivas Gannavaram, Thalia Pacheco-Fernandez, Timur Oljuskin, Ranadhir Dey, Abhay R. Satoskar, Hira L. Nakhasi

“…Our laboratory has developed several live attenuated <i>Leishmania</i> vaccines by targeted <i>centrin</i> gene disruptions either by homologous recombination or, more recently, by using genome editing technologies involving CRISPR-Cas9. …”
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Effects of cellulase and Lactiplantibacillus plantarum on the fermentation quality, microbial diversity, gene function prediction, and in vitro rumen fermentation parameters of Car... by Ji Ju, Guijie Zhang, Ming Xiao, Chenyang Dong, Runze Zhang, Liu Du, Yongjie Zheng, Manli Wei, Manlin Wei, Baiyila Wu

“…Metabolism of terpenoids and polyketides, mismatch repair, DNA replication, nucleotide excision repair, and homologous recombination increased in the CL group compared with those in the CK group. …”
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Nuclear Export Inhibitor KPT-8602 Synergizes with PARP Inhibitors in Escalating Apoptosis in Castration Resistant Cancer Cells by Md. Hafiz Uddin, Yiwei Li, Husain Yar Khan, Irfana Muqbil, Amro Aboukameel, Rachel E. Sexton, Shriya Reddy, Yosef Landesman, Trinayan Kashyap, Asfar S. Azmi, Elisabeth I. Heath

“…KPT-8602 with or without olaparib was shown to reduce homologous recombination-regulated DNA damage response targets including <i>BRCA1</i>, <i>BRCA2</i>, <i>CHEK1</i>, <i>EXO1</i>, <i>BLM</i>, <i>RAD51</i>, <i>LIG1</i>, <i>XRCC3</i> and <i>RMI2</i>. …”
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Identification of a chromatin regulator signature and potential prognostic ability for adrenocortical carcinoma by Junwu Li, Yuanzhen Jia, Lin Tang, Ronggui Zhang, Yuanfeng Zhang

“…Differentially expressed CRs (DECRs) were mostly associated with the cell cycle, base excision repair, colon cancer, gene duplication, homologous recombination, and other signaling pathways for the high-risk group. …”
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Transcriptome Analysis by RNA Sequencing of Mouse Embryonic Stem Cells Stocked on International Space Station for 1584 Days in Frozen State after Culture on the Ground by Kayo Yoshida, Megumi Hada, Masami Hayashi, Akane Kizu, Kohei Kitada, Kiyomi Eguchi-Kasai, Toshiaki Kokubo, Takeshi Teramura, Hiromi Hashizume Suzuki, Hitomi Watanabe, Gen Kondoh, Aiko Nagamatsu, Premkumar Saganti, Masafumi Muratani, Francis A. Cucinotta, Takashi Morita

“…The expressions of most of the genes that were involved in homologous recombination (HR) and non-homologous end joining (NHEJ) were not significantly changed between the ISS-stocked cells and ground-stocked control cells. …”
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COMMD1, from the Repair of DNA Double Strand Breaks, to a Novel Anti-Cancer Therapeutic Target by Amila Suraweera, Pascal H. G. Duijf, Christian Jekimovs, Karsten Schrobback, Cheng Liu, Mark N. Adams, Kenneth J. O’Byrne, Derek J. Richard

“…DNA repair assays demonstrated that COMMD1 is required for the efficient repair of DSBs and reporter assays showed that COMMD1 functions in both non-homologous-end-joining and homologous recombination. Bioinformatic analysis showed that <i>COMMD1</i> is upregulated in NSCLC, with high levels of <i>COMMD1</i> associated with poor patient prognosis. …”
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Precision Oncology of High-Grade Ovarian Cancer Defined through Targeted Sequencing by Sandra Wessman, Beatriz Bohorquez Fuentes, Therese Törngren, Anders Kvist, Georgia Kokaraki, Hanna Menkens, Elisabet Hjerpe, Ythalo Hugo da Silva Santos, Tirzah Braz Petta, Åke Borg, Joseph W. Carlson

“…Mutations were identified in <i>TP53</i>, <i>BRCA1</i>, <i>BRCA2</i>, as well as additional homologous recombination repair pathway genes <i>BARD1</i>, <i>ATR</i>, <i>CHEK2</i>, <i>PALB2</i>, <i>RAD51D</i>, <i>RAD50</i>, <i>SLX4</i>, <i>FANCA</i>, <i>RAD51C</i>, and <i>RAD54L</i>. …”
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Successful Treatment of Concurrent Follicular Lymphoma and Triple-Negative Breast Cancer Using Rituximab Plus Nab-Paclitaxel and Cisplatin: A Case Report and Literature Review by Zhu Z, Zhou N, Yu S, Gao X, Cheng X, Wang Y, Bai C

“…According to the next-generation sequencing (NGS) assay on breast mass showing a homologous recombination repair (HRR) deficiency (HRD) score of 72, the neoadjuvant regimen was changed to rituximab plus nab-paclitaxel and cisplatin (R-TP) and resulted in significant tumor regression. …”
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Clinical and genomic features of non‐small cell lung cancer occurring in families by Shingo Miyabe, Shin Ito, Ikuro Sato, Jiro Abe, Keiichi Tamai, Mai Mochizuki, Haruna Fujimori, Kazunori Yamaguchi, Norihisa Shindo, Hiroshi Shima, Tomoko Yamazaki, Makoto Abue, Yoshinori Okada, Jun Yasuda

“…Delineation of mutational signatures of the somatic SNVs with deconstructSigs for the pair of germline pathogenic DHODH variant‐positive cases showed that the mutational signatures of these cases included SBS3 (homologous recombination repair defect), SBS6, 15 (DNA mismatch repair), and SBS7 (ultraviolet exposure), suggesting that disordered pyrimidine production causes increased errors in DNA repair systems in these cases. …”
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OncoPan^®: An NGS-Based Screening Methodology to Identify Molecular Markers for Therapy and Risk Assessment in Pancreatic Ductal Adenocarcinoma by Maria Grazia Tibiletti, Ileana Carnevali, Valeria Pensotti, Anna Maria Chiaravalli, Sofia Facchi, Sara Volorio, Frederique Mariette, Paolo Mariani, Stefano Fortuzzi, Marco Alessandro Pierotti, Fausto Sessa

“…A proportion of 5–10% of PDAC have a hereditary origin involving germline variants of homologous recombination genes, such as Mismatch Repair (MMR), <i>STK11</i> and <i>CDKN2A</i> genes. …”
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HuR Plays a Role in Double-Strand Break Repair in Pancreatic Cancer Cells and Regulates Functional BRCA1-Associated-Ring-Domain-1(BARD1) Isoforms by Aditi Jain, Matthew McCoy, Carolyn Coats, Samantha Z. Brown, Sankar Addya, Carl Pelz, Rosalie C. Sears, Charles J. Yeo, Jonathan R. Brody

“…Silencing HuR caused a >70% decrease in homologous recombination repair (HRR) efficiency as measured by the double-strand break repair (pDR-GFP reporter) assay. …”
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