Supercoil Levels in E. coli and Salmonella Chromosomes Are Regulated by the C-Terminal 35–38 Amino Acids of GyrA by Nikolay S. Rovinskiy, Andrews A. Agbleke, Olga N. Chesnokova, N. Patrick Higgins

“…Negative supercoils influence DNA replication, transcription, homologous recombination, site-specific recombination, genetic transposition and sister chromosome segregation. …”
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Short-term genome evolution of Listeria monocytogenes in a non-controlled environment by Orsi, Renato H., Borowsky, Mark L., Lauer, Peter, Young, Sarah K., Nusbaum, Chad, Galagan, James E., Birren, Bruce W., Ivy, Reid A., Sun, Qi, Graves, Lewis M., Swaminathan, Bala, Wiedmann, Martin

“…Diversification of this prophage appears to have been caused by multiple homologous recombination events or possibly prophage replacement. …”
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Integrated genomic analyses of ovarian carcinoma by Meyerson, Matthew L., Lander, Eric Steven

“…Pathway analyses suggested that homologous recombination is defective in about half of the tumours analysed, and that NOTCH and FOXM1 signalling are involved in serous ovarian cancer pathophysiology.…”
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A Gene Regulatory Program for Meiotic Prophase in the Fetal Ovary by Junker, Jan Philipp, Gill, Mark E., Mueller, Jacob L., van Oudenaarden, Alexander, Soh, Ying Qi Shirleen, Page, David C

“…The chromosomal program of meiotic prophase, comprising events such as laying down of meiotic cohesins, synapsis between homologs, and homologous recombination, must be preceded and enabled by the regulated induction of meiotic prophase genes. …”
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Type 1 insulin-like growth factor receptor inhibition as treatment for urological cancer by Chitnis, M, Dr. Meenali M. Chitnis

“…The second aim was to investigate effects of IGF-1R inhibition on radiosensitivity and DNA repair, following previous work in our laboratory showing that IGF-1R depletion enhances chemo- and radio-sensitivity, delays double strand break (DSB) resolution, and may play a role in the homologous recombination (HR) pathway of DNA DSB repair. However, the repair defect seen in these early experiments was larger than could be entirely explained by a defect in HR. …”

Intersecting doublesex neurons underlying sexual behaviours in Drosophila melanogaster by Pavlou, H

“…To achieve this, I generated a novel split-GAL4 <em>dsx</em>^GAL4-DBD hemidriver by ends in homologous recombination. Coupling the novel tool with the pan-neuronal <em>elav</em>^VP16-AD hemidriver, revealed spatial restriction of <em>dsx</em>^GAL4-DBD/<em>elav</em>^VP16-AD expression to <em>dsx</em> neurons only; enabling the realisation of novel patterns of <em>dsx</em>-expression in the peripheral NS.…”

Investigating the mechanisms responsible for DNA double-strand break-induced loss of heterozygosity in fission yeast by Cullen, J, Jason Kingsley Cullen

“…This study has identified an additional role for the MRN complex, Rad22 and RPA in such break-induced translocations, suggesting that both allelic crossovers and BIR require homologous recombination (HR) in fission yeast. Surprisingly, break-induced extensive LOH was still observed in HR mutants. …”

Metabolic engineering of Bacillus amyloliquefaciens for efficient production of α-glucosidase inhibitor1-deoxynojirimycin by Xujie Li, Meng Zhang, Yu Lu, Ningyang Wu, Jian'gang Chen, Zhixia Ji, Yangyang Zhan, Xin Ma, Junyong Chen, Dongbo Cai, Shouwen Chen

“…Firstly, using the temperature-sensitive shuttle plasmid T2 (2)-Ori, gene ptsG in phosphotransferase system (PTS) was weakened by homologous recombination, and non-PTS pathway was strengthened by deleting its repressor gene iolR, and 1-DNJ yield of resultant strain HZ-S2 was increased by 4.27-fold, reached 110.72 mg/L. …”
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Nuclear localization of human MEIOB requires its NLS in the OB domain and interaction with SPATA22 by Xu Yating, Chen Zhen, Wu Panfeng, Qu Wei, Shi Hanqing, Cheng Muyang, Xu Yumin, Jin Tingyu, Liu Cong, Liu Cong, Li Yi, Luo Mengcheng

“…MEIOB is a vital protein in meiotic homologous recombination and plays an indispensable role in human gametogenesis. …”
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CRISPR/Cas9-Mediated Gene Correction in Osteopetrosis Patient-Derived iPSCs by Dandan Li, Minglin Ou, Wei Zhang, Qi Luo, Wanxia Cai, Chune Mo, Wenken Liang, Guandong Dai, Lianghong Yin, Peng Zhu, Donge Tang, Yong Dai

“…Methods: Based on our previously established osteopetrosis-specific iPSCs (ADO2-iPSCs), we repaired the point mutation R286W of the CLCN7 gene in ADO2-iPSCs by the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) mediated homologous recombination. Results: The obtained gene corrected ADO2-iPSCs (GC-ADO2-iPSCs) were characterized in terms of hESC-like morphology, a normal karyotype, expression of pluripotency markers, homozygous repaired sequence of CLCN7 gene, and the ability to differentiate into cells of three germ layers. …”
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Coexistence of Multidrug Resistance and Virulence in a Single Conjugative Plasmid from a Hypervirulent Klebsiella pneumoniae Isolate of Sequence Type 25 by Peiwen Xia, Miao Yi, Yaling Yuan, Jinzhu Huang, Bingxue Yang, Jiajia Liao, Zijun Dang, Shengli Luo, Yun Xia

“…Moreover, virulence plasmids with complete conjugation modules have been identified, which evolved via homologous recombination. Here, we characterize an ST25 CR-hvKP strain, CY814036, harboring both a conjugative MDR-virulence plasmid and a blaKPC-2-bearing plasmid in China. …”
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Model integrating CT-based radiomics and genomics for survival prediction in esophageal cancer patients receiving definitive chemoradiotherapy by Jinfeng Cui, Li Li, Ning Liu, Wenhong Hou, Yinjun Dong, Fengchang Yang, Shouhui Zhu, Jun Li, Shuanghu Yuan

“…Multivariate analysis demonstrated that the Rad-score and homologous recombination repair (HRR) pathway alterations were independent prognostic factors correlating with PFS. …”
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In Vitro Model for a Drug Assessment of Cytochrome P450 Family 3 Subfamily A Member 4 Substrates Using Human Induced Pluripotent Stem Cells and Genome Editing Technology by Sayaka Deguchi, Tomohiro Shintani, Kazuo Harada, Toru Okamoto, Akinori Takemura, Kazumasa Hirata, Kousei Ito, Kazuo Takayama, Hiroyuki Mizuguchi

“…We previously developed procedures to differentiate human induced pluripotent stem (iPS) cells into hepatocyte‐like cells (HLCs) or intestinal epithelial‐like cells (IECs) with a fetal phenotype as well as a highly efficient genome editing technology that could enhance the homologous recombination efficiency at any locus, including CYP3A4. …”
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Radio-resistance of hypoxic tumors: exploring the effects of oxygen and X-ray radiation on non-small lung cancer cell lines by Rachel Hanley, Francesca Pagliari, Daniel Garcia-Calderón, Joana Fernandes Guerreiro, Géraldine Genard, Jeannette Jansen, Clelia Nisticò, Maria Grazia Marafioti, Luca Tirinato, Joao Seco

“…The extent of irradiation (IR)-induced DNA damage was evaluated by analyzing γ-H2AX foci induction and the altered expression of repair genes involved in non-homologous end joining and homologous recombination pathways. Moreover, cell-altered responses were investigated, including the nuclear and cytosolic hydrogen peroxide (H2O2) production, as well as the associated anti-oxidant potential, in particular some components related to the glutathione system. …”
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The necroptosis related gene LGALS3 can be used as a biomarker for the adverse progression from chronic HBV infection to HCC by Jianming Dong, Rongzheng Zhang, Yan Xia, Xu Jiang, Kun Zhou, Kun Zhou, Jiaqi Li, Mengrui Guo, Xinyang Cao, Shuyun Zhang

“…The HBV-HCC cell model was established by transfection of pAAV/HBV1.2C2, constructed by homologous recombination, into HUH7 and HEPG2 cells. The expression levels of G6PD, PINK1, and LGALS3 were detected using RT-qPCR. …”
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Exploiting Synthetic Lethality between Germline BRCA1 Haploinsufficiency and PARP Inhibition in JAK2V617F-Positive Myeloproliferative Neoplasms by Max Bermes, Maria Jimena Rodriguez, Marcelo Augusto Szymanski de Toledo, Sabrina Ernst, Gerhard Müller-Newen, Tim Henrik Brümmendorf, Nicolas Chatain, Steffen Koschmieder, Julian Baumeister

“…RAD51 foci formation was impaired in <i>Brca1</i>^+/− cells compared to <i>Brca1</i>^+/+ cells, indicating impaired homologous recombination repair due to <i>Brca1</i> haploinsufficiency. …”
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Genomic Landscape of Normal and Breast Cancer Tissues in a Hungarian Pilot Cohort by Orsolya Pipek, Donát Alpár, Orsolya Rusz, Csaba Bödör, Zoltán Udvarnoki, Anna Medgyes-Horváth, István Csabai, Zoltán Szállási, Lilla Madaras, Zsuzsanna Kahán, Gábor Cserni, Bence Kővári, Janina Kulka, Anna Mária Tőkés

“…For many samples, the somatic mutational landscape was dominated by mutational processes associated with homologous recombination deficiency (HRD). Our study, as the first breast tumor/normal sequencing study in Hungary, revealed several aspects of the significantly mutated genes and mutational signatures, and some of the copy number variations and somatic fusion events. …”
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Whole-exome sequencing of calcitonin-producing pancreatic neuroendocrine neoplasms indicates a unique molecular signature by Claudia Döring, Katharina Peer, Katrin Bankov, Carmen Bollmann, Annette Ramaswamy, Pietro Di Fazio, Peter Johannes Wild, Peter Johannes Wild, Detlef Klaus Bartsch

“…Immunohistochemistry showed protein expression of MUC4 in two cases and MUC16 in one case, and the liver metastasis of a third case was double positive for MUC4 and MUC16. The homologous recombination deficiency (HRD) score of all tumors was low.DiscussionCT-pNENs have a unique molecular signature compared to other pNEN subtypes, specifically involving the FGFR4, DPYD, MUC4, MUC16 and the KRT family genes. …”
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A versatile 2A peptide-based strategy for ectopic expression and endogenous gene tagging in Trypanosoma cruzi by Gabriela T. Niemirowicz, Giannina Carlevaro, Oscar Campetella, León A. Bouvier, Juan Mucci

“…In addition, these vectors could easily be transformed into endogenous tagging constructs that allowed the insertion, by ends-in homologous recombination, of a hemagglutinin tag (HA) fused to the actin gene. …”
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Evaluation of topotecan and 10-hydroxycamptothecin on Toxoplasma gondii: Implications on baseline DNA damage and repair efficiency by Constanza Cristaldi, Ana M. Saldarriaga Cartagena, Agustina Ganuza, William J. Sullivan, Jr., Sergio O. Angel, Laura Vanagas

“…DSBs are mainly repaired by Homologous Recombination Repair (HRR) and Non-Homologous End Joining (NHEJ). …”
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