p21-activated kinase is involved in the sporulation, pathogenicity, and stress response of Arthrobotrys oligospora under the indirect regulation of Rho GTPase-activating protein by Meichen Zhu, Meichen Zhu, Yankun Liu, Yankun Liu, Xuewei Yang, Xuewei Yang, Lirong Zhu, Lirong Zhu, Yanmei Shen, Yanmei Shen, Shipeng Duan, Shipeng Duan, Jinkui Yang, Jinkui Yang

“…In addition, we successfully deleted Aopak1 and Aorga1 via the homologous recombination method. The disruption of Aopak1 and Aorga1 caused a remarkable reduction in spore yield and the number of nuclei per cell, but did not affect mycelial growth. …”
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Prognostic stratification of endometrial cancers with high microsatellite instability or no specific molecular profile by Jesus Gonzalez-Bosquet, S. John Weroha, Jamie N. Bakkum-Gamez, Amy L. Weaver, Michaela E. McGree, Sean C. Dowdy, Abimbola O. Famuyide, Benjamin R. Kipp, Kevin C. Halling, Siddhartha Yadav, Fergus J. Couch, Fergus J. Couch, Karl C. Podratz

“…Clinical outcomes were annotated after integrating ECPPF and sequence variations in homologous recombination (HR) genes.ResultsData were available for 239 patients with EC, which included 58 MSI-H and 89 NSMP cases. …”
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An EGFP Knock-in Zebrafish Experimental Model Used in Evaluation of the Amantadine Drug Safety During Early Cardiogenesis by Shi Ouyang, Shi Ouyang, Wu-Ming Qin, Wu-Ming Qin, Yu-Juan Niu, Yu-Juan Niu, Yong-He Ding, Yong-He Ding, Yun Deng, Yun Deng

“…Here, we report teratogenic effects of amantadine drug during early cardiogenesis through developing a novel zebrafish (Danio rerio) knock-in (KI) animal model and explore the underlying mechanisms.MethodsHomologous recombination (HR) pathway triggered by CRISPR/Cas9 system was utilized to generate an enhanced green fluorescent protein (EGFP) KI zebrafish animal model. …”
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Sensitization of chondrosarcoma cells with PARP inhibitor and high-LET radiation by Mathieu Césaire, Utpal Ghosh, Jean-Baptiste Austry, Etienne Muller, Francesco Paolo Cammarata, Marilyne Guillamin, Massimo Caruso, Laurent Castéra, Giada Petringa, Giuseppe Antonio Pablo Cirrone, François Chevalier

“…The radiosensitivity of CH2879 cells was associated with mutations in homologous recombination repair genes, such as RAD50, SMARCA2 and NBN. …”
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Salvia chinensis Benth Inhibits Triple-Negative Breast Cancer Progression by Inducing the DNA Damage Pathway by Kai-nan Wang, Ye Hu, Lin-lin Han, Shan-shan Zhao, Chen Song, Si-wen Sun, Hui-yun Lv, Ni-na Jiang, Ling-zhi Xv, Zuo-wei Zhao, Man Li

“…In particular, SCH particularly blocked the inter-strand cross-link repair mechanism and the double-strand breach repair caused by the homologous recombination pathway, in addition to inducing DNA damage. …”
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Characterization of <i>bla</i>_KPC-2-Carrying Plasmid pR31-KPC from a <i>Pseudomonas aeruginosa</i> Strain Isolated in China by Min Yuan, Hongxia Guan, Dan Sha, Wenting Cao, Xiaofeng Song, Jie Che, Biao Kan, Juan Li

“…The further transfer of <i>bla</i>_KPC-2 in these plasmids may be initiated by either the Tn<i>3</i> family or IS<i>26</i>-associated transposition or homologous recombination. The data presented here will contribute to a deeper understanding of <i>bla</i>_KPC-2 carrying plasmids in <i>Pseudomonas</i>.…”
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Understanding the Genetic Landscape of Pancreatic Ductal Adenocarcinoma to Support Personalized Medicine: A Systematic Review by Antonino Pantaleo, Giovanna Forte, Candida Fasano, Martina Lepore Signorile, Paola Sanese, Katia De Marco, Elisabetta Di Nicola, Marialaura Latrofa, Valentina Grossi, Vittoria Disciglio, Cristiano Simone

“…A high proportion of these patients (<i>n</i> = 1225/1493, 82%) were found to harbor alterations in genes (<i>ATM</i>, <i>BRCA1</i>, <i>BRCA2</i>, <i>PALB2</i>) involved in the homologous recombination repair (HRR) pathway. Specifically, the remaining PDAC patients were reported to carry alterations in genes playing a role in other cancer pathways (<i>CDKN2A</i>, <i>STK11</i>, <i>TP53</i>; <i>n</i> = 181/1493, 12.1%) or in the mismatch repair (MMR) pathway (<i>MLH1</i>, <i>MSH2</i>, <i>MSH6</i>, <i>PMS2</i>; <i>n</i> = 87/1493, 5.8%). …”
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MEK1 drives oncogenic signaling and interacts with PARP1 for genomic and metabolic homeostasis in malignant pleural mesothelioma by Haitang Yang, Yanyun Gao, Duo Xu, Ke Xu, Shun-Qing Liang, Zhang Yang, Amina Scherz, Sean R. R. Hall, Stefan Forster, Sabina Berezowska, Feng Yao, Adrian F. Ochsenbein, Thomas M. Marti, Gregor J. Kocher, Ralph A. Schmid, Patrick Dorn, Ren-Wang Peng

“…Mechanistically, MEKi induces impairment of homologous recombination (HR) repair proficiency and mitochondrial metabolic activity, which is counterbalanced by pleiotropic PARP1. …”
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SPOP Deregulation Improves the Radiation Response of Prostate Cancer Models by Impairing DNA Damage Repair by Rihan El Bezawy, Martina Tripari, Stefano Percio, Alessandro Cicchetti, Monica Tortoreto, Claudio Stucchi, Stella Tinelli, Valentina Zuco, Valentina Doldi, Paolo Gandellini, Riccardo Valdagni, Nadia Zaffaroni

“…SPOP silencing also resulted in a significant downregulation of RAD51 and CHK1 expression, consistent with the impairment of homologous recombination. Our results indicate that SPOP deregulation plays a radiosensitizing role in PCa by impairing DDR via downregulation of RAD51 and CHK1.…”
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Study on the Role of ampG in the Regulation of Plasmid-Mediated ampC -Induced Expression in Klebsiella pneumoniae by Li G, Wang L, Zhang H, Luan Y, Sun Q, Duo L

“…Guiling Li,1 Li Wang,2 Heguang Zhang,1 Ying Luan,1 Qi Sun,1 Libo Duo1 1Department of Clinical Laboratory, the Second Hospital Affiliated to Harbin Medical University, Harbin, Heilongjiang, 150086, People’s Republic of China; 2Department of Clinical Laboratory, School of Medicine, Chengdu Women’s and Children’s Central Hospital, University of Electronic Science and Technology of China, Chengdu, Sichuan, 611731, People’s Republic of ChinaCorrespondence: Libo Duo, Department of Clinical Laboratory, the Second Hospital affiliated to Harbin Medical University, No. 246 XueFu Road, NanGang District, Harbin, Heilongjiang, 150086, People’s Republic of China, Tel +86 045186605382, Email duolibo@163.comObjective: In this study, we constructed ampG knock-out and knock-in strains from a clinically isolated Kp1strain carrying ampR-ampC in its plasmid and compared them with the Kp NTUH-K2044 strain to investigate the relationship between ampG and ampR-ampC-induced expression.Methods: We created the ampG gene deletion mutant strains Kp1-ΔampG and Kp NTUH-K2044-ΔampG with pKO3-km plasmid using homologous recombination technology. We constructed the Kp NTUH-K2044-RC and Kp NTUH-K2044-ΔampG-RC drug resistance model strains with plasmid pACYC184. …”
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Tradeoffs of microbial life history strategies drive the turnover of microbial-derived organic carbon in coastal saline soils by Qi Ning, Lin Chen, Fang Li, Guixiang Zhou, Congzhi Zhang, Donghao Ma, Jiabao Zhang, Jiabao Zhang

“…However, high-salinity soil is dominated by stress tolerance (S) (e.g., higher metabolic functions of homologous recombination, base excision repair, biofilm formation, extracellular polysaccharide biosynthesis, and osmolytes production) and resource acquisition (A) strategies (e.g., higher alkaline phosphatase activity, transporters, and flagellar assembly). …”
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Interspecies transmission of porcine-originated G4P[6] rotavirus A between pigs and humans: a synchronized spatiotemporal approach by Valentina Kunić, Tina Mikuletič, Rok Kogoj, Tom Koritnik, Andrej Steyer, Silvija Šoprek, Goran Tešović, Goran Tešović, Vlatka Konjik, Ivana Roksandić Križan, Marina Prišlin, Lorena Jemeršić, Dragan Brnić

“…The origin of chimeric human–animal strains of RVA is closely linked to the crucial role of mixed genotypes in driving reassortment and homologous recombination, which play a major role in shaping the genetic diversity of RVA. …”
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Carbon Catabolite Repression Gene <i>AoCreA</i> Regulates Morphological Development and Ochratoxin A Biosynthesis Responding to Carbon Sources in <i>Aspergillus ochraceus</i> by Gang Wang, Yulong Wang, Bolei Yang, Chenxi Zhang, Haiyong Zhang, Fuguo Xing, Yang Liu

“…To give an insight into the mechanism of the carbon sources regulating development of <i>A. ochraceus</i> and OTA production, we have identified <i>AoCreA</i> in <i>A. ochraceus</i>. The homologous recombination strategy was used to generate the <i>AoCreA</i> deletion mutant (Δ<i>AoCreA</i>). …”
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Optical Genome Mapping Reveals the Landscape of Structural Variations and Their Clinical Significance in HBOC-Related Breast Cancer by Yanan Cheng, Li Dong, Dechao Bu, Lei Han, Yi Zhao, Juntian Liu, Xiaojing Guo, Hanli Xu, Jinpu Yu

“…SVhigh tumors were associated with higher Ki-67 expression, higher homologous recombination deficiency (HRD) scores, more mutated genes, and altered signaling pathways. …”
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Development of a Lung Vacancy Mouse Model through CRISPR/Cas9-Mediated Deletion of Thyroid Transcription Factor 1 Exon 2 by Lihua Zhao, Meishuang Li, Zhibao Yin, Limin Lv, Meng Zhou, Yixi Wang, Manling Zhang, Tianxu Guo, Xiyun Guo, Han Liu, Linxin Cheng, Xiubin Liang, Shuguang Duo, Rongfeng Li

“…<i>TTF-1</i> knockout mice were originally produced by inserting a stop codon in Exon 3 of the gene (E3stop) through embryonic stem cell-based homologous recombination. The main problems of utilizing E3stop host embryos for lung SCOG are that these animals all have a tracheoesophageal fistula (TEF), which cannot be corrected by donor stem cells, and most of them have monolateral sac-like lungs. …”
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A Novel CDK4/6 and PARP Dual Inhibitor ZC-22 Effectively Suppresses Tumor Growth and Improves the Response to Cisplatin Treatment in Breast and Ovarian Cancer by Chenchen Tian, Yufan Wei, Jianjun Li, Zhi Huang, Qiong Wang, Yingxue Lin, Xingping Lv, Yanan Chen, Yan Fan, Peiqing Sun, Rong Xiang, Antao Chang, Shuang Yang

“…Current studies have shown therapeutic synergy for combinations of PARPi and CDK4/6i in breast and ovarian cancers with homologous recombination (HR) proficiency, which represents a new synthetic lethal strategy for treatment of these cancers regardless HR status. …”
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Sumoylation-deficient phosphoglycerate mutase 2 impairs myogenic differentiation by Yi Zhang, Yi Zhang, Ilimbek Beketaev, Yanlin Ma, Yanlin Ma, Jun Wang

“…Given that K176 is more highly evolutionarily conserved across paralogs and orthologs than K49 is, we used the CRISPR-mediated homologous recombination technique in myogenic C2C12 cells to generate homozygous K176R knock-in cells (PGAM2K176R/K176R). …”
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Synergistic targeting of BRCA1 mutated breast cancers with PARP and CDK2 inhibition by Diar Aziz, Neil Portman, Kristine J. Fernandez, Christine Lee, Sarah Alexandrou, Alba Llop-Guevara, Zoe Phan, Aliza Yong, Ashleigh Wilkinson, C. Marcelo Sergio, Danielle Ferraro, Dariush Etemadmoghadam, David D. Bowtell, kConFab Investigators, Violeta Serra, Paul Waring, Elgene Lim, C. Elizabeth Caldon

“…CDK2 inhibition induced DNA damage and synergized with PARP inhibitors to reduce cell viability in cell lines with homologous recombination deficiency, including BRCA1 mutated cell lines. …”
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Topological Analysis of γH2AX and MRE11 Clusters Detected by Localization Microscopy during X-ray-Induced DNA Double-Strand Break Repair by Hannes Hahn, Charlotte Neitzel, Olga Kopečná, Dieter W. Heermann, Martin Falk, Michael Hausmann

“…MRE11 is part of the MRE11–RAD50–NBS1 (MRN) complex, which is known as a DNA strand resection and broken-end bridging component in homologous recombination repair (HRR) and alternative non-homologous end joining (a-NHEJ). …”
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The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci by Joshua C D'Aeth, Mark PG van der Linden, Lesley McGee, Herminia de Lencastre, Paul Turner, Jae-Hoon Song, Stephanie W Lo, Rebecca A Gladstone, Raquel Sá-Leão, Kwan Soo Ko, William P Hanage, Robert F Breiman, Bernard Beall, Stephen D Bentley, Nicholas J Croucher, The GPS Consortium

“…Multidrug-resistant Streptococcus pneumoniae emerge through the modification of core genome loci by interspecies homologous recombinations, and acquisition of gene cassettes. …”
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