Achog1 is required for the asexual sporulation, stress responses and pigmentation of Aspergillus cristatus by Lei Shao, Yumei Tan, Yumei Tan, Shiying Song, Shiying Song, Yuchen Wang, Yuchen Wang, Yongxiang Liu, Yongxiang Liu, Yonghui Huang, Yonghui Huang, Xiyi Ren, Xiyi Ren, Zuoyi Liu, Zuoyi Liu, Zuoyi Liu

“…Deletion mutants of Achog1 were obtained by homologous recombination. Phenotypic observations showed that the time required to produce conidia was delayed, and the number of conidia produced was significantly reduced in the deletion mutants of Achog1 in hypertonic media, indicating that Achog1 plays a positive role in asexual development. …”
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A novel CRISPR/Cas9 system with high genomic editing efficiency and recyclable auxotrophic selective marker for multiple-step metabolic rewriting in Pichia pastoris by Xiang Wang, Yi Li, Zhehao Jin, Xiangjian Liu, Xiang Gao, Shuyuan Guo, Tao Yu

“…However, it is difficult to use P. pastoris as the chassis cell factory for high-valuable metabolite biosynthesis due to the low homologous recombination (HR) efficiency and the limitation of handy selective markers, especially in the condition of multistep biosynthetic pathways. …”
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Molecular Features and Clinical Management of Hereditary Pancreatic Cancer Syndromes and Familial Pancreatic Cancer by Akiyoshi Kasuga, Takeshi Okamoto, Shohei Udagawa, Chinatsu Mori, Takafumi Mie, Takaaki Furukawa, Yuto Yamada, Tsuyoshi Takeda, Masato Matsuyama, Takashi Sasaki, Masato Ozaka, Arisa Ueki, Naoki Sasahira

“…Signature 3 identified by whole genome sequencing is also associated with homologous recombination deficiency and sensitivity to targeted therapies. …”
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A Strategy for the Rapid Development of a Safe <i>Vibrio cholerae</i> Candidate Vaccine Strain by Dmitry S. Karpov, Anna V. Goncharenko, Evgenii V. Usachev, Daria V. Vasina, Elizaveta V. Divisenko, Yaroslava M. Chalenko, Andrei A. Pochtovyi, Roman S. Ovchinnikov, Valentin V. Makarov, Sergei M. Yudin, Artem P. Tkachuk, Vladimir A. Gushchin

“…However, the genomic engineering of wild-type <i>V. cholerae</i> strains is difficult, and these strains are genetically unstable due to their high homologous recombination activity. We comprehensively characterized two <i>V. cholerae</i> isolates using genome sequencing, bioinformatic analysis, and microscopic, physiological, and biochemical tests. …”
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The nitric oxide synthase gene negatively regulates biofilm formation in Staphylococcus epidermidis by Jiaxue Wang, Jiaxue Wang, Jiaxue Wang, Jiaxue Wang, Lulin Rao, Zhuoan Huang, Lili Ma, Tian Yang, Zhongqi Yu, Aihua Sun, Yumei Ge, Yumei Ge, Yumei Ge, Yumei Ge

“…Bioinformatics analysis of the NOS gene was performed, and homologous recombination was subsequently employed to delete this gene. …”
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Comprehensive analyses of genomic features and mutational signatures in adenosquamous carcinoma of the lung by Hongbiao Wang, Jun Liu, Sujuan Zhu, Kun Miao, Zhifeng Li, Xiaofang Qi, Lujia Huang, Lijie Guo, Yan Wang, Yuyin Cai, Yingcheng Lin

“…We found that mutations ARID2, BRCA1, and KEAP1 were associated with high TMB. The homologous recombination repair (HRR) pathway-related gene mutation displayed a slightly higher TMB than those without mutations. …”
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The efficacy and safety of niraparib for ovarian cancer: a single-center observational study from China by Jing Ni, Xianzhong Cheng, Qian Zhao, Zhiqin Dai, Xia Xu, Wenwen Guo, Hongyuan Gu, Rui Zhou, Yan Wang, Xiaoxiang Chen

“…Abstract Background Niraparib, a poly (ADP-ribose) polymerase (PARP) inhibitor, is approved for first/second-line maintenance treatment of ovarian cancer patients with complete or partial response to platinum-based chemotherapy, and multi-line monotherapy in BRCAmt patients or platinum-sensitive recurrence patients with homologous recombination deficiency (HRD). We present real-world experience from a single center of China. …”
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High-grade serous ovarian carcinoma organoids as models of chromosomal instability by Maria Vias, Lena Morrill Gavarró, Carolin M Sauer, Deborah A Sanders, Anna M Piskorz, Dominique-Laurent Couturier, Stéphane Ballereau, Bárbara Hernando, Michael P Schneider, James Hall, Filipe Correia-Martins, Florian Markowetz, Geoff Macintyre, James D Brenton

“…We show that HGSOC PDOs comprise communities of different clonal populations and represent models of different causes of chromosomal instability including homologous recombination deficiency, chromothripsis, tandem-duplicator phenotype, and whole genome duplication. …”
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DNA repair pathways-targeted cyclovirobuxine inhibits castration-resistant prostate cancer growth by promoting cell apoptosis and cycle arrest by Ke Gao, Siying Zhu, Qiuju Shao, Yuhong Qi, Chao Zhang, Xiaoshun Li, Jiaheng Guo, Guojun Wu, Hanbing Jiang

“…Mechanistically, it primarily regulated BRCA1-, POLD1-, BLM-, MSH2-, MSH6- and PCNA-mediated mismatch repair, homologous recombination repair, base excision repair, Fanconi anemia repair, and nucleotide excision repair pathways. …”
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Examining the Diagnostic Yield of Tumour Testing and Qualifying Germline Concordance for Hereditary Cancer Variants in Patients with High-Grade Serous Carcinoma by Emily A. Goebel, Jennifer Kerkhof, Oleksandra Dzyubak, C. Meg McLachlin, Jacob McGee, Bekim Sadikovic

“…Genomic alterations in the homologous recombination (HR) pathway are used for cancer risk assessment and render tumours sensitive to platinum-based chemotherapy and poly (ADP-ribose) polymerase inhibitors (PARPi), which can be associated with more favourable outcomes. …”
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Genomic analysis of plasma circulating tumor DNA in patients with heavily pretreated HER2 + metastatic breast cancer by Kyoungmin Lee, Jongwon Lee, Jungmin Choi, Sung Hoon Sim, Jeong Eun Kim, Min Hwan Kim, Yeon Hee Park, Jee Hyun Kim, Su-Jin Koh, Kyong Hwa Park, Myoung Joo Kang, Mi Sun Ahn, Kyoung Eun Lee, Hee-Jun Kim, Hee Kyung Ahn, Han Jo Kim, Keon Uk Park, In Hae Park

“…The frequency of homologous recombination deficiency (HRD)-related gene mutations was higher than that in matched tumor tissues, and these mutations tended to be associated with shorter PFS. …”
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Molecular characterization of coxsackievirus B5 from the sputum of pneumonia children patients of Kunming, Southwest China by Miao Tan, Jiale Suo, Zhilei Zhang, Wenji He, Li Tan, Haiyan Jiang, Ming Li, Juan He, Yue Pan, Bin Xu, Lingmei Yan, Songtao Bin, Zhengyan Gan, Yuxing Sun, Hongchao Jiang, Qiangming Sun, Zhen Zhang

“…Results A total of five CVB5 complete genome sequences were obtained. No obvious homologous recombination signals comparing with other coxsackie B viruses were observed in the five isolates. …”
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Efficient Mutagenesis of Marek’s Disease Virus-Encoded microRNAs Using a CRISPR/Cas9-Based Gene Editing System by Jun Luo, Man Teng, Xusheng Zai, Na Tang, Yaoyao Zhang, Ahmedali Mandviwala, Vishwanatha R. A. P. Reddy, Susan Baigent, Yongxiu Yao, Venugopal Nair

“…Interestingly, mutagenesis of the targeted miRNAs showed changes in the in vitro virus growth kinetics, which is consistent with that of the in vivo proliferation curves of our previously reported GX0101 mutants produced by the bacterial artificial chromosome (BAC) clone and Rec E/T homologous recombination techniques. Our data demonstrate that the CRISPR/Cas9-based gene editing is a simple, efficient and relatively nondisruptive approach for manipulating the small non-coding genes from the genome of herpesvirus and will undoubtedly contribute significantly to the future progress in herpesvirus biology.…”
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301 Inhibition of lysine-specific histone demethylase 1A (KDM1A/LSD1) attenuates DNA double strand break repair and enhances efficacy of temozolomide in glioblastoma by Salvador Alejo, Bridgitte Palacios, Prabhakar Pitta Venkata, Yi He, Wenjing Li, Jessica Johnson, Sridharan Jayamohan, Ratna Vadlamudi, Gangadhara Sareddy

“…Mechanistic studies were conducted using CUT&Tag-seq, RNA-seq, immunofluorescence, comet, Western blotting, RT-qPCR, homologous recombination (HR) or non-homologous end-joining (NHEJ) DNA repair reporter assays. …”
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Functional assessment of missense variants of uncertain significance in the cancer susceptibility gene PALB2 by Shijie Wu, Lina Qi, Huihui Chen, Kun Zhang, Jiapan He, Xianan Guo, Lu Shen, Yunxiang Zhou, Xi Zhong, Shu Zheng, Jiaojiao Zhou, Yiding Chen

“…We showed that three PALB2 VUSs p.K16M [c.47 A > T], p.L24F [c.72 G > C], and p.L35F [c.103 C > T] in the coiled-coil domain impaired PALB2-mediated homologous recombination. The p.L24F and p.L35F variants partially disrupted BRCA1-PALB2 interactions, reduced RAD51 foci formation in response to DNA damage, abrogated ionizing radiation-induced G2/M checkpoint maintenance, and conferred increased sensitivity to olaparib and cisplatin. …”
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STC2 activates PRMT5 to induce radioresistance through DNA damage repair and ferroptosis pathways in esophageal squamous cell carcinoma by Kan Jiang, Xin Yin, Qingyi Zhang, Jie Yin, Qiuying Tang, Mengyou Xu, Lingyun Wu, Yifan Shen, Ziyang Zhou, Hao Yu, Senxiang Yan

“…Mechanistically, STC2 can promote DDR through the homologous recombination and non-homologous end joining pathways by activating PRMT5. …”
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The effects of lotus-fish co-culture on the gut microbiome of Hefang crucian carp (Carassis auratus) by Shengnan Li, Qianhong Gu, Zhanxin Li, Qianqian Zeng, Hui Zhong, Meiqi Liu, Jiaoyu Chen, Yi Zhou, Shaojun Liu, Shengbiao Hu

“…On the contrary, the bacterial functional potentials related to nucleotide metabolism, DNA mismatch repair, as well as homologous recombination were significantly stimulated in the IPC system. …”
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Recombinant Production of MFHR1, A Novel Synthetic Multitarget Complement Inhibitor, in Moss Bioreactors by Oguz Top, Oguz Top, Juliana Parsons, Lennard L. Bohlender, Stefan Michelfelder, Phillipp Kopp, Christian Busch-Steenberg, Sebastian N. W. Hoernstein, Peter F. Zipfel, Karsten Häffner, Ralf Reski, Ralf Reski, Ralf Reski, Eva L. Decker

“…The key features of this system are precise genome engineering via homologous recombination, Good Manufacturing Practice-compliant production in photobioreactors, high batch-to-batch reproducibility, and product stability. …”
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Genome-wide identification and analysis of WD40 proteins in wheat (Triticum aestivum L.) by Rui Hu, Jie Xiao, Ting Gu, Xiaofen Yu, Yang Zhang, Junli Chang, Guangxiao Yang, Guangyuan He

“…As the main driving force of evolution, duplication events were observed, and homologous recombination was another driving force of evolution. …”
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Insertion of a FLAG-tag sequence at the end of exon 9 of the TBX5 gene in three induced pluripotent stem cell lines (DHMi004-A-4, DHMi004-A-5, DHMi004-A-6) by CRISPR/Cas9 technolog... by Harald Lahm, Niraj K. Singh, Irina Gottmann, Stefanie A. Doppler, Elda Dzilic, Heike Preisler, Stephanie Schneider, Rüdiger Lange, Markus Krane, Martina Dreßen

“…Employing CRISPR/Cas9 technology, we have inserted a FLAG-tag sequence at the end of exon 9 of the TBX5 gene prior to the stop codon by homologous recombination. The translated TBX5-FLAG fusion protein of the three iPSC lines can effectively be precipitated by anti-FLAG antibodies and, thus, allow the detection of specific TBX5-binding sites and their associated genes.…”
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