HopE and HopD Porin-Mediated Drug Influx Contributes to Intrinsic Antimicrobial Susceptibility and Inhibits Streptomycin Resistance Acquisition by Natural Transformation in Helicob... by Yixin Liu, Feng Yang, Su Wang, Wenjing Chi, Li Ding, Tao Liu, Feng Zhu, Danian Ji, Jun Zhou, Yi Fang, Jinghao Zhang, Ping Xiang, Yanmei Zhang, Hu Zhao

“…IMPORTANCE Helicobacter pylori is constitutively competent for natural transformation (NT) and possesses an efficient system for homologous recombination, which could be utilized to study the NT-mediated horizontal gene transfer induced antibiotic resistance acquisition. …”
Get full text

Mathematical modeling of the early modeled CA-125 longitudinal kinetics (KELIM-PARP) as a pragmatic indicator of rucaparib efficacy in patients with recurrent ovarian carcinoma in... by Olivier Colomban, Elizabeth M. Swisher, Rebecca Kristeleit, Iain McNeish, Ronnie Shapira-Frommer, Sandra Goble, Kevin K. Lin, Lara Maloney, Gilles Freyer, Benoit You

“…The prognostic value of KELIM-PARP regarding treatment efficacy (radiological response, and progression-free survival (PFS)) was assessed using univariable/multivariable analyses, with respect to platinum-sensitivity and homologous recombination deficiency (HRD) status. Findings: The data from 476 patients were assessed. …”
Get full text

IS1294 Reorganizes Plasmids in a Multidrug-Resistant Escherichia coli Strain by Yushan Pan, Tengli Zhang, Lijie Yu, Zhiyong Zong, Shiyu Zhao, Ruichao Li, Qianqian Wang, Li Yuan, Gongzheng Hu, Dandan He

“…IS26, present in many antibiotic-resistant plasmids from Gram-negative bacteria, plays a critical role in the spread, clustering, and reorganization of resistance determinant-encoding plasmids and in plasmid reorganization through replicative transposition mechanisms and homologous recombination. However, the role of IS1294, present in many MDR plasmids, in the formation of cointegrates remains unclear. …”
Get full text

Pharmacological inhibition of BAP1 recruits HERC2 to competitively dissociate BRCA1–BARD1, suppresses DNA repair and sensitizes CRC to radiotherapy by Xin Yue, Tingyu Liu, Xuecen Wang, Weijian Wu, Gesi Wen, Yang Yi, Jiaxin Wu, Ziyang Wang, Weixiang Zhan, Ruirui Wu, Yuan Meng, Zhirui Cao, Liyuan Le, Wenyan Qiu, Xiaoyue Zhang, Zhenyu Li, Yong Chen, Guohui Wan, Xianzhang Bu, Zhenwei Peng, Ran-yi Liu

“…The BRCA1–BARD1 complex has been demonstrated to play a critical role in homologous recombination (HR) DSB repair, and its functions may be affected by HERC2 or BAP1. …”
Get full text

Hereditary Gastric Cancer: Single-Gene or Multigene Panel Testing? A Mono-Institutional Experience by Mariarosaria Calvello, Monica Marabelli, Sara Gandini, Elena Marino, Loris Bernard, Matteo Dal Molin, Giulia Di Cola, Cristina Zanzottera, Giovanni Corso, Nicola Fazio, Lorenzo Gervaso, Uberto Fumagalli Romario, Massimo Barberis, Aliana Guerrieri-Gonzaga, Lucio Bertario, Davide Serrano, Bernardo Bonanni

“…With the spread of multigene panel testing (MGPT), a debate arose on the involvement of other genes, particularly those pertaining to homologous recombination (HR) repair. We report our mono-institutional experience in genetic counseling and SGT for 54 GC patients, with the detection of nine pathogenic variants (PVs) (9/54:16.7%). …”
Get full text

The disruption of the CCDC6 – PP4 axis induces a BRCAness like phenotype and sensitivity to PARP inhibitors in high-grade serous ovarian carcinoma by Francesco Morra, Francesco Merolla, Giovanna Damia, Francesca Ricci, Silvia Varricchio, Gennaro Ilardi, Laura Arenare, Daniela Califano, Virginia Napolitano, Robert Fruscio, Rosa Marina Melillo, Luca Palazzo, Angela Celetti

“…Abstract Background Treatment with PARP inhibitors (PARPi) is primarily effective against high-grade serous ovarian cancers (HGSOC) with BRCA1/2 mutations or other deficiencies in homologous recombination (HR) repair mechanisms. However, resistance to PARPi frequently develops, mostly as a result of BRCA1/2 reversion mutations. …”
Get full text

Improving Ergometrine Production by <i>easO</i> and <i>easP</i> Knockout in <i>Claviceps paspali</i> by Yun-Ming Qiao, Yan-Hua Wen, Ting Gong, Jing-Jing Chen, Tian-Jiao Chen, Jin-Ling Yang, Ping Zhu

“…In this study, gene knockout of <i>C. paspali</i> by homologous recombination yielded two mutants ∆<i>easO_hetero</i>-1 and ∆<i>easP_hetero</i>-34 with ergometrine titers of 1559.36 mg∙L⁻¹ and 837.57 mg∙L⁻¹, which were four and two times higher than that of the wild-type control, respectively. …”
Get full text

TRAIL Receptor Targeting Agents Potentiate PARP Inhibitor Efficacy in Pancreatic Cancer Independently of <i>BRCA2</i> Mutation Status by Najib Ben Khaled, Katharina Hammer, Liangtao Ye, Ahmed Alnatsha, Sebastian A. Widholz, Ignazio Piseddu, Simon Sirtl, Julia Schneider, Stefan Munker, Ujjwal Mukund Mahajan, Juan José Montero, Joscha Griger, Julia Mayerle, Florian P. Reiter, Enrico N. De Toni

“…Investigations of the genetic features of PDAC have demonstrated a frequent occurrence of mutations in genes involved in homologous recombination (HR), especially in the breast cancer susceptibility gene 2 (<i>BRCA2</i>). …”
Get full text

Deleting the C84L Gene from the Virulent African Swine Fever Virus SY18 Does Not Affect Its Replication in Porcine Primary Macrophages but Reduces Its Virulence in Swine by Jinjin Yang, Rongnian Zhu, Yanyan Zhang, Xintao Zhou, Huixian Yue, Qixuan Li, Junnan Ke, Yu Wang, Faming Miao, Teng Chen, Fei Zhang, Shoufeng Zhang, Aidong Qian, Rongliang Hu

“…In this study, we identified one of the unknown functional genes, C84L. (2) Methods: A gene deficient strain was obtained through homologous recombination and several rounds of purification, and its replication characteristics and virulence were studied through in vitro and in vivo experiments, respectively. (3) Results: Deleting this gene from the wild-type virulent strain SY18 did not affect its replication in porcine primary macrophages but reduced its virulence in pigs. …”
Get full text

The Rice AAA-ATPase OsFIGNL1 Is Essential for Male Meiosis by Peipei Zhang, Peipei Zhang, Yingxin Zhang, Lianping Sun, Sittipun Sinumporn, Zhengfu Yang, Bin Sun, Dandan Xuan, Zihe Li, Ping Yu, Weixun Wu, Kejian Wang, Liyong Cao, Shihua Cheng

“…Although several genes involved in homologous recombination and DNA repair have been reported, their functions in rice (Oryza sativa) male meiosis remain poorly understood. …”
Get full text

Pesticin-Like Effector VgrG3cp Targeting Peptidoglycan Delivered by the Type VI Secretion System Contributes to Vibrio cholerae Interbacterial Competition by Ming Liu, Meng-Yu Zhao, Heng Wang, Zeng-Hang Wang, Zhao Wang, Ying Liu, Yin-Peng Li, Tao Dong, Yang Fu

“…Bioinformatic analysis showed that genes encoding VgrG3cp-like homologs are distributed in Vibrio species, are linked with T6SS structural genes and auxiliary genes, and the vgrG3cp-tsiV3cp gene pair of V. cholerae probably evolved from Vibrio anguillarum and Vibrio fluvialis via homologous recombination. Through a time-lapse microscopy assay, we directly determined that cells accumulating VgrG3cp disrupted bacterial division, while the cells continued to increase in size until the loss of membrane potential and cell wall breakage and finally burst. …”
Get full text

5-Fluorouracil/L-Leucovorin Plus Oxaliplatin (FOLFOX) Regimen as Salvage Chemotherapy for Patients with Unresectable Pancreatic Cancer Receiving Gemcitabine and Nab-Paclitaxel and... by Takuo Yamai, Kenji Ikezawa, Yasuharu Kawamoto, Takeru Hirao, Sena Higashi, Kazuma Daiku, Shingo Maeda, Yutaro Abe, Makiko Urabe, Yugo Kai, Ryoji Takada, Tasuku Nakabori, Hiroyuki Uehara, Kazuyoshi Ohkawa

“…Two patients with controlled disease had homologous recombination deficiency (HRD)-associated gene mutations in cancer panel testing. …”
Get full text

FAM135B sustains the reservoir of Tip60‐ATM assembly to promote DNA damage response by Kai Zhang, Qingnan Wu, Wenzhong Liu, Yan Wang, Lianmei Zhao, Jie Chen, Haoyu Liu, Siqi Liu, Jinting Li, Weimin Zhang, Qimin Zhan

“…Consistently, our findings revealed that FAM135B could promote homologous recombination and non‐homologous end‐joining repairs. …”
Get full text

Implementing HRD Testing in Routine Clinical Practice on Patients with Primary High-Grade Advanced Ovarian Cancer by Florian Heitz, Beyhan Ataseven, Claudia Staniczok, Carsten Denkert, Kerstin Rhiem, Eric Hahnen, Sebastian Heikaus, Malak Moubarak, Julia Welz, Timoleon Dagres, Vasilios Vrentas, Mareike Bommert, Stephanie Schneider, Nicole Concin, Philipp Harter

“…The chemotherapy backbone for patients with high-grade advanced epithelial ovarian cancer (HG-AOC) is carboplatin and paclitaxel followed by a maintenance therapy either with bevacizumab, with a PARP inhibitor, or with a combination of both, which is defined by the presence of a homologous recombination deficiency (HRD) and by the <i>BRCA1/2</i> status. …”
Get full text

RAD51 Is Implicated in DNA Damage, Chemoresistance and Immune Dysregulation in Solid Tumors by Chengcheng Liao, Srikanth Talluri, Jiangning Zhao, Shidai Mu, Subodh Kumar, Jialan Shi, Leutz Buon, Nikhil C. Munshi, Masood A. Shammas

“…Background: In normal cells, homologous recombination (HR) is tightly regulated and plays an important role in the maintenance of genomic integrity and stability through precise repair of DNA damage. …”
Get full text

<i>CUL4A</i>, <i>ERCC5</i>, and <i>ERCC1</i> as Predictive Factors for Trabectedin Efficacy in Advanced Soft Tissue Sarcomas (STS): A Spanish Group for Sarcoma Research (GEIS) Stud... by David S. Moura, Paloma Sanchez-Bustos, Antonio Fernandez-Serra, María Lopez-Alvarez, José L. Mondaza-Hernandez, Elena Blanco-Alcaina, Angela Gavilan-Naranjo, Paula Martinez-Delgado, Serena Lacerenza, Paloma Santos-Fernandez, Irene Carrasco-Garcia, Samuel Hidalgo-Rios, Antonio Gutierrez, Rafael Ramos, Nadia Hindi, Miguel Taron, Jose Antonio Lopez-Guerrero, Javier Martin-Broto

“…A translational study was designed to analyze the expression of nucleotide excision repair (NER) and homologous recombination (HR) genes as potential predictive biomarkers for trabectedin in soft-tissue sarcoma (STS). …”
Get full text

A novel DNA damage quantification platform enables high throughput screening for genes that impact DNA double strand breaks by Tay, Ian Jun Jie.

Get full text

Mutational topography reflects clinical neuroblastoma heterogeneity by Elias Rodriguez-Fos, Mercè Planas-Fèlix, Martin Burkert, Montserrat Puiggròs, Joern Toedling, Nina Thiessen, Eric Blanc, Annabell Szymansky, Falk Hertwig, Naveed Ishaque, Dieter Beule, David Torrents, Angelika Eggert, Richard P. Koche, Roland F. Schwarz, Kerstin Haase, Johannes H. Schulte, Anton G. Henssen

“…Whereas high-risk MYCN-amplified neuroblastomas were characterized by signs of replication slippage and stress, homologous recombination-associated signatures defined high-risk non-MYCN-amplified patients. …”
Get full text

Set2 family regulates mycotoxin metabolism and virulence via H3K36 methylation in pathogenic fungus Aspergillus flavus by Zhenhong Zhuang, Xiaohua Pan, Mengjuan Zhang, Yaju Liu, Chuanzhong Huang, Yu Li, Ling Hao, Shihua Wang

“…By construction of domain deletion A. flavus strain and point mutation strains by homologous recombination, the study revealed that SET domain is indispensable in mycotoxin anabolism and virulence of A. flavus, and N455 and V457 in it are the key amino acid residues. …”
Get full text

A novel ferroptosis-related gene prognostic index for prognosis and response to immunotherapy in patients with prostate cancer by Yuliang Wang, Yuliang Wang, Jiaqi Fan, Jiaqi Fan, Tao Chen, Lele Xu, Pengyu Liu, Lijia Xiao, Tao Wu, Qingchun Zhou, Qingyou Zheng, Chunxiao Liu, Franky Leung Chan, Dinglan Wu, Dinglan Wu

“…In addition, comprehensive results demonstrated that high FRGPI scores showed a significantly positive correlation with worse clinicopathological features, higher mutation counts, increased frequency of copy number variations (CNVs), higher homologous recombination deficiency (HRD) and immune scores, higher mRNAsi, and more importantly, enhanced sensitivity to immunotherapy.ConclusionsFRGPI is not only a promising and robust prognostic biomarker, but also a potential indicator of immunotherapeutic outcomes for PCa patients after radical prostatectomy.…”
Get full text