Investigation of Molecular Features Involved in Clinical Responses and Survival in Advanced Endometrial Carcinoma Treated by Hormone Therapy by Mathias Neron, Arnaud Guille, Lucie Allegre, Pierre-Emmanuel Colombo, Cristina Leaha, José Adelaide, Nadine Carbuccia, Frédéric Courtier, Florence Boissiere, Evelyne Crapez, Michel Fabbro, Sébastien Gouy, Emilie Mamessier, Éric Lambaudie, Daniel Birnbaum, François Bertucci, Max Chaffanet

“…The genomic profiles of 34 tumors showed an average altered genome of 3.26%, DNA repair homologous recombination deficiency in five tumors (14.7%), and 17 regions significantly targeted by amplification/deletion. …”
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Development and utility of SSR markers based on Brassica sp. whole-genome in triangle of U by Nairan Sun, Nairan Sun, Nairan Sun, Jisuan Chen, Yuqi Wang, Yuqi Wang, Yuqi Wang, Iqbal Hussain, Iqbal Hussain, Na Lei, Xinyan Ma, Xinyan Ma, Weiqiang Li, Weiqiang Li, Weiqiang Li, Kaiwen Liu, Kaiwen Liu, Hongrui Yu, Hongrui Yu, Kun Zhao, Kun Zhao, Tong Zhao, Tong Zhao, Yi Zhang, Yi Zhang, Xiaolin Yu, Xiaolin Yu, Xiaolin Yu

“…The reasons may be partial gene dominated chromosomal homologous recombination and rearrangement during the evolution of basic diploid species into allotetraploids. …”
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27-hydroxycholesterol and DNA damage repair: implication in prostate cancer by Gloria Cecilia Galvan, Nadine A. Friedrich, Sanjay Das, Sanjay Das, Sanjay Das, James P. Daniels, Sara Pollan, Shweta Dambal, Ryusuke Suzuki, Sergio E. Sanders, Sungyong You, Hisashi Tanaka, Hisashi Tanaka, Yeon-Joo Lee, Wei Yuan, Johann S. de Bono, Johann S. de Bono, Irina Vasilevskaya, Karen E. Knudsen, Michael R. Freeman, Stephen J. Freedland, Stephen J. Freedland

“…DNA damage was assessed via comet assays.ResultsTranscriptome analysis revealed 27HC treatment downregulated Hallmark pathways related to DNA damage repair, decreased expression of FEN1 and RAD51, and induced “BRCAness” by downregulating genes involved in homologous recombination regulation in LNCaP cells. Consistently, we found a correlation between higher CYP27A1 expression (i.e., higher intracellular 27HC) and decreased expression of DNA repair gene signatures in castration-sensitive PC (CSPC) in human PC datasets. …”
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Differential DNA damage repair and PARP inhibitor vulnerability of the mammary epithelial lineages by Hyeyeon Kim, Kazeera Aliar, Pirashaanthy Tharmapalan, Curtis W. McCloskey, Abhijith Kuttanamkuzhi, Barbara T. Grünwald, Luis Palomero, Mathepan J. Mahendralingam, Matthew Waas, Arvind S. Mer, Mitchell J. Elliott, Bowen Zhang, Khalid N. Al-Zahrani, Ellen R. Langille, Michael Parsons, Swami Narala, Stefan Hofer, Paul D. Waterhouse, Razqallah Hakem, Benjamin Haibe-Kains, Thomas Kislinger, Daniel Schramek, David W. Cescon, Miquel A. Pujana, Hal K. Berman, Rama Khokha

“…Summary: It is widely assumed that all normal somatic cells can equally perform homologous recombination (HR) and non-homologous end joining in the DNA damage response (DDR). …”
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Efficacy of recombinant Newcastle disease virus expressing HA protein of H9N2 Avian influenza virus in respiratory and intestinal tract by Guanming Shao, Zi Xie, Ming Liang, Yaxin Liu, Chaoyi Song, Keyu Feng, Xinheng Zhang, Wencheng Lin, Jun Fu, Qingmei Xie

“…In this study, based on Red/ET homologous recombination technology, H9N2 AIV HA gene was embedded into the genome of NDV LaSota vaccine strain to obtain the recombinant virus rNDV-H9. …”
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A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models by Ding, Qiurong, Lee, Youn-Kyoung, Schaefer, Esperance A.K., Peters, Derek T., Veres, Adrian, Kim, Kevin, Kuperwasser, Nicolas, Motola, Daniel L., Meissner, Torsten B., Hendriks, William T., Trevisan, Marta, Gupta, Rajat M., Moisan, Annie, Banks, Eric, Friesen, Max, Schinzel, Robert T., Xia, Fang, Tang, Alexander, Xia, Yulei, Figueroa, Emmanuel, Wann, Amy, Ahfeldt, Tim, Daheron, Laurence, Zhang, Feng, Rubin, Lee L., Peng, Lee F., Chung, Raymond T., Musunuru, Kiran, Cowan, Chad A.

“…Classical gene-targeting technology via homologous recombination has proven to be an invaluable tool of experimental biology through its use in mouse embryonic stem cells for generating germline knockout and knockin mice; however, its use in mammalian systems has been limited primarily to studies in mice. …”
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Nivolumab plus rucaparib for metastatic castration-resistant prostate cancer: results from the phase 2 CheckMate 9KD trial by Jun Li, Stefanie Zschäbitz, Daniel P Petrylak, Margitta Retz, Prabhu Bhagavatheeswaran, Marc-Oliver Grimm, Jose Perez-Gracia, Gwenaelle Gravis, Karim Fizazi, Jeffrey C Goh, Mauricio Burotto, Daniel Castellano, Fred Saad, Andrew J Armstrong, HAKIM MAHAMMEDI, Russell K Pachynski, Louis Lacombe, Diogo Assed Bastos, Steven L McCune, Juan Carlos Vázquez Limón, Edmond M Kwan, Aude Fléchon, David R Shaffer, Neha P Amin, Keziban Ünsal-Kaçmaz, Xuya Wang, Andrea Loehr

“…Coprimary endpoints were objective response rate (ORR) per Prostate Cancer Clinical Trials Working Group 3 and prostate-specific antigen response rate (PSA50-RR; ≥50% PSA reduction) in all-treated patients and patients with homologous recombination deficiency (HRD)-positive tumors, determined before enrollment. …”
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PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage responseResearch in context by Wei Yue, Xinyu Li, Xiaolu Zhan, Lei Wang, Jihong Ma, Meiyu Bi, Qilong Wang, Xiaoyang Gu, Bingteng Xie, Tong Liu, Hongyan Guo, Xin Zhu, Chen Song, Jie Qiao, Mo Li

“…However, recent clinical trials reported patients’ survival benefits from PARP inhibitor treatments, irrelevant to homologous recombination deficiency. Therefore, revealing the therapeutic mechanism of PARP inhibitors beyond DNA damage repair is urgently needed, which can facilitate precision medicine. …”
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Multicenter Real-World Data of Subsequent Chemotherapy after Progression to PARP Inhibitors in a Maintenance Relapse Setting by Margarita Romeo, Marta Gil-Martín, Lydia Gaba, Iris Teruel, Álvaro Taus, Claudia Fina, Maria Masvidal, Paola Murata, Julen Fernández-Plana, Alejandro Martínez, Cristina Pérez, Yolanda García, Valerie Rodriguez, Sara Cros, Marta Parera, Montserrat Zanui, Silvia Catot, Beatriz Pardo, Andrea Plaja, Anna Esteve, Maria Pilar Barretina-Ginesta

“…Results: 111 patients were included [46 (41.4%) presented pathological BRCA1/2 mutations, 8 (7.5%) in other homologous recombination-related genes]. Sixty-four patients (57.7%) had received two prior chemotherapy lines, including the one immediately prior to PARPi. …”
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Genome stability : DNA repair and recombination / by Haber, James E., author

“…An overview of the four major pathways of homologous recombinational repair is followed by a description of the several mechanisms of nonhomologous end-joining. …”

A novel GC-rich dispersed repeat sequence in Drosophila melanogaster. by Flavell, A, Dyson, J, Ish-Horowicz, D

“…Southern blotting shows that these repeats are not transposable elements. Twenty four homologous recombinants have been localised by in situ hybridization to seven sites in the Drosophila genome. …”

Crossover promotion and prevention. by Lorenz, A, Whitby, M

“…Homologous recombination is an important mechanism for the repair of double-strand breaks in DNA. …”

Making crossovers during meiosis. by Whitby, M

“…Homologous recombination (HR) is required to promote both correct chromosome segregation and genetic variation during meiosis. …”

Stabilization of RAD51 nucleoprotein filaments by the C-terminal region of BRCA2. by Esashi, F, Galkin, V, Yu, X, Egelman, E, West, S

“…The human breast cancer susceptibility gene BRCA2 is required for the regulation of RAD51-mediated homologous recombinational repair. BRCA2 interacts with RAD51 monomers, as well as nucleoprotein filaments, primarily though the conserved BRC motifs. …”

Yeast recombinational cloning for heterologous biosynthesis of polyketides: a molecular microbiology laboratory module for undergraduate students by Mihaela Bejenari, Line Nielsen, Eva Mie Lang Spedtsberg, Mikkel Rank Nielsen, Tobias Bruun Pedersen, Jens Laurids Sørensen

“…Among the multitude of available plasmid assembly strategies, in vivo homologous recombinational cloning in yeast has emerged as a cost-effective and relatively simple method. …”
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Genetic Susceptibility to Endometrial Cancer: Risk Factors and Clinical Management by Thilo Dörk, Peter Hillemanns, Clemens Tempfer, Julius Breu, Markus C. Fleisch

“…Germline variants and somatic events may act in concert to modulate the molecular evolution of the tumor, where mismatch-repair deficiency is common in endometrioid endometrial tumors whereas homologous recombinational repair deficiency has been described for non-endometrioid endometrial tumors. …”
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Development Of Gene Deleted Recombinant Pseudorabies Virus by Alauddin, Zeenathul Nazariah

“…A gE gene-deleted pseudorabies virus (TK¯gE¯PrV) was constructed by homologous recombinational techniques. The TK¯gE+PrV, regarded as the parental strain in the study, originated from a virulent local PrV isolate (TK+gE+PrV). …”
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Functional Markers for Precision Plant Breeding by Romesh K. Salgotra, C. Neal Stewart

“…Functional genomics approaches such as transcriptomics, targeting induced local lesions in genomes (TILLING), homologous recombinant (HR), association mapping, and allele mining are all strategies to identify FMs for breeding goals, such as agronomic traits and biotic and abiotic stress resistance. …”
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Requirement for Schizosaccharomyces pombe Top3 in the maintenance of chromosome integrity. by Win, T, Goodwin, A, Hickson, I, Norbury, C, Wang, S

“…Our data are consistent with the notion that Top3 has a key function in homologous recombinational repair during S phase that is essential for ensuring subsequent fidelity of chromosome segregation.…”

Plk1 and CK2 act in concert to regulate Rad51 during DNA double strand break repair. by Yata, K, Lloyd, J, Maslen, S, Bleuyard, J, Skehel, M, Smerdon, S, Esashi, F

“…Homologous recombination (HR) plays an important role in the maintenance of genome integrity. …”