T-Cell Receptor Repertoire Characteristics Associated with Prognostic Significance in High-Grade Serous Ovarian Carcinoma by Ju-Won Kim, Sewha Kim, So-Yun Yang, Je-Gun Joung, Sohyun Hwang

“…The patient’s clinical characteristics, gene expression pattern, TCR clonotypes, and degree of tumor-infiltrating leukocytes (TILs) were analyzed, and the patients were divided into groups depending on their recurrence pattern, tumor-infiltrating leukocyte (TIL) score, and homologous recombinant repair pathway deficiency (HRD)-associated mutations. …”
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CRISPR Screens in Synthetic Lethality and Combinatorial Therapies for Cancer by Laia Castells-Roca, Eudald Tejero, Benjamín Rodríguez-Santiago, Jordi Surrallés

“…More recently, prompted by the clinical success of the synthetic lethality (SL)-based therapy of the PARP inhibitors in homologous recombinant deficient tumors, scientists have centered their novel research on SL interactions (SLI). …”
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Replication fork blockage by RTS1 at an ectopic site promotes recombination in fission yeast. by Ahn, J, Osman, F, Whitby, M

“…Homologous recombination is believed to play important roles in processing stalled/blocked replication forks in eukaryotes. …”

The transcriptional basis of chromosome pairing. by Cook, P

“…Pairing between homologous chromosomes is essential for successful meiosis; generally only paired homologs recombine and segregate correctly into haploid germ cells. …”

Sequence Analysis of the Complete Mitochondrial Genome of a Medicinal Plant, <i>Vitex rotundifolia</i> Linnaeus f. (Lamiales: Lamiaceae) by Xiaoli Yu, Zhonggang Duan, Yanjun Wang, Qingxin Zhang, Wei Li

“…A multipartite structure mediated by the homologous recombinations of the three direct repeats was found in the <i>V. rotundifolia</i> mitochondrial genome. …”
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Divergence, recombination and retention of functionality during protein evolution by Xu Yanlong O, Hall Randall W, Goldstein Richard A, Pollock David D

“…Here, we present results on the retention of functionality in homologous recombinants following population divergence. A central result is that protein structure characteristics can strongly influence recombinant functionality. …”
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The interstitial response to renal injury: fibroblast-like cells show phenotypic changes and have reduced potential for erythropoietin gene expression. by Maxwell, P, Ferguson, D, Nicholls, L, Johnson, M, Ratcliffe, P

“…To define the potential for erythropoietin gene expression in injured kidneys, marker gene expression was examined in transgenic mice bearing a homologously recombined erythropoietin--simian virus 40 T antigen (Epo-TAg) transgene. …”

Genetic alterations of KRAS and TP53 in intrahepatic cholangiocarcinoma associated with poor prognosis by Peng Jianbo, Fang Shuo, Li Meisheng, Liu Yuxin, Liang Xiaolu, Li Zuobiao, Chen Gaohui, Peng Lijiao, Chen Nianping, Liu Lei, Xu Xiaohong, Dai Wei

“…DNA damage repair and homologs recombinant repair deficiencies were significantly associated with high TMB in ICCA cases. …”
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A Streamlined Approach for Fluorescence Labelling of Low-Copy-Number Plasmids for Determination of Conjugation Frequency by Flow Cytometry by Qin Qi, Muhammad Kamruzzaman, Jonathan R. Iredell

“…A blue fluorescent protein gene is inserted into a conjugative plasmid of interest using a simple homologous recombineering procedure. A small non-conjugative plasmid, which carries a red fluorescent protein gene with a toxin–antitoxin system that functions as a plasmid stability module, is used to label the recipient bacterial strain. …”
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Visualization of direct and diffusion-assisted RAD51 nucleation by full-length human BRCA2 protein by Belan, O, Greenhough, L, Kuhlen, L, Anand, R, Kaczmarczyk, A, Gruszka, DT, Yardimci, H, Zhang, X, Rueda, DS, West, SC, Boulton, SJ

“…Homologous recombination (HR) is essential for error-free repair of DNA double-strand breaks, perturbed replication forks (RFs), and post-replicative single-stranded DNA (ssDNA) gaps. …”

Conformational flexibility revealed by the crystal structure of a crenarchaeal RadA by Ariza, A, Richard, D, White, M, Bond, C

“…Homologous recombinational repair is an essential mechanism for repair of double-strand breaks in DNA. …”

Wheat, Rye, and Barley Genomes Can Associate during Meiosis in Newly Synthesized Trigeneric Hybrids by María-Dolores Rey, Carmen Ramírez, Azahara C. Martín

“…One of the biggest challenges faced by a new polyploid is meiosis, in particular, discriminating between multiple related chromosomes so that only homologs recombine to ensure regular chromosome segregation and fertility. …”
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Mutagenesis and functional studies with succinate dehydrogenase inhibitors in the wheat pathogen Mycosphaerella graminicola. by Gabriel Scalliet, Judith Bowler, Torsten Luksch, Lucy Kirchhofer-Allan, Diana Steinhauer, Keith Ward, Michael Niklaus, Andreas Verras, Michael Csukai, Antoine Daina, Raymonde Fonné-Pfister

“…In order to explore the impact of mutations on pathogen fitness in vivo and in planta, homologous recombinants were generated for a selection of mutation types. …”
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PARP1 Inhibitor and Trabectedin Combination Does Not Increase Tumor Mutational Burden in Advanced Sarcomas—A Preclinical and Translational Study by Ymera Pignochino, Giovanni Crisafulli, Giorgia Giordano, Alessandra Merlini, Enrico Berrino, Maria Laura Centomo, Giulia Chiabotto, Silvia Brusco, Marco Basiricò, Elena Maldi, Alberto Pisacane, Valeria Leuci, Dario Sangiolo, Lorenzo D’Ambrosio, Massimo Aglietta, Bernd Kasper, Alberto Bardelli, Giovanni Grignani

“…Whole-exome and targeted panel sequencing data analyses revealed that three cycles of trabectedin and olaparib combination neither affected the mutational profiles, DNA repair gene status, or copy number alterations, nor increased TMB both in homologous recombinant-defective and proficient cells or in xenografts. …”
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Olfactory receptor genes and chromosome 11 structural aberrations: Players or spectators? by Serena Redaelli, Francesca Romana Grati, Viviana Tritto, Giuliana Giannuzzi, Maria Paola Recalcati, Elena Sala, Nicoletta Villa, Francesca Crosti, Gaia Roversi, Francesca Malvestiti, Valentina Zanatta, Elena Repetti, Ornella Rodeschini, Chiara Valtorta, Ilaria Catusi, Lorenza Romitti, Emanuela Martinoli, Donatella Conconi, Leda Dalprà, Marialuisa Lavitrano, Paola Riva, Angela Bentivegna

“…We found that OR genes are not involved in chromosome 11 reciprocal translocations, suggesting that different DNA motifs and mechanisms based on homology or non-homology recombination can cause chromosome 11 structural alterations. …”
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F-box DNA Helicase 1 (FBH1) Contributes to the Destabilization of DNA Damage Repair Machinery in Human Cancers by Alizhah J. Watson, Michaela L. Shaffer, Renee A. Bouley, Ruben C. Petreaca

“…Homologous recombination (HR) is the major mechanism of rescue of stalled replication forks or repair of DNA double-strand breaks (DSBs) during S phase or mitosis. …”
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A Novel Recombinant FAdV-4 Virus with Fiber of FAdV-8b Provides Efficient Protection against Both FAdV-4 and FAdV-8b by Hao Lu, Quan Xie, Wei Zhang, Jianjun Zhang, Weikang Wang, Mingjun Lian, Zhehong Zhao, Dan Ren, Songhua Xie, Yun Lin, Tuofan Li, Yaru Mu, Zhimin Wan, Hongxia Shao, Aijian Qin, Jianqiang Ye

“…In this study, recombinant FAdV-4 expressing the fiber of FAdV-8b, designated as FA4-F8b, expressing fiber of FAdV-8b was generated by the CRISPR-Cas9 and homologous recombinant techniques. Infection studies in vitro and in vivo revealed that the FA4-F8b replicated efficiently in LMH cells and was also highly pathogenic to 2-week-old SPF chickens. …”
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Site-directed mutagenesis of Mycobacterium tuberculosis and functional validation to investigate potential bedaquiline resistance-causing mutations by Christian C. Otum, Emmanuel Rivière, Monique Barnard, Johannes Loubser, Monique J. Williams, Elizabeth M. Streicher, Annelies Van Rie, Robin M. Warren, Marisa Klopper

“…We introduced two mutations, atpE Ile66Val and Rv0678 Thr33Ala, in the Mycobacterium tuberculosis H37Rv reference strain using homologous recombineering or recombination to investigate the phenotypic effect of these mutations. …”
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DNA repair deficiency as circulating biomarker in prostate cancer by Martina Catalano, Daniele Generali, Marta Gatti, Barbara Riboli, Leda Paganini, Gabriella Nesi, Giandomenico Roviello

“…Deleterious aberrations in DNA repair genes are actionable in approximately 25% of metastatic castration-resistant prostate cancers (mCRPC) patients. Homology recombination repair (HRR) is the DNA damage repair (DDR) mechanism most frequently altered in prostate cancer; of note BRCA2 is the most frequently altered DDR gene in this tumor. …”
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Whole Genome Resequencing Revealed the Effect of Helicase <i>yqhH</i> Gene on Regulating <i>Bacillus thuringiensis</i> LLP29 against Ultraviolet Radiation Stress by Weibo Ma, Xiong Guan, Ying Miao, Lingling Zhang

“…In order to further verify its function, the <i>yqhH</i> gene was knocked out and complemented by homologous recombinant gene knockout technology. The survival rate of the knockout mutant strain Bt LLP29-Δ<i>yqhH</i> was significantly lower than that of the original strain Bt LLP29 and the back-complemented strain Bt LLP29-Δ<i>yqhH</i>-R after treated with UV. …”
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