Identification of New Potential Prognostic and Predictive Markers in High-Grade Osteosarcoma Using Whole Exome Sequencing by Raffaele Gaeta, Mariangela Morelli, Francesca Lessi, Chiara Maria Mazzanti, Michele Menicagli, Rodolfo Capanna, Lorenzo Andreani, Luca Coccoli, Paolo Aretini, Alessandro Franchi

“…In particular, BRCA2 and RAD50 are involved in homologous recombination repair, and could thus be used as specific therapy targets of inhibitors of the enzyme Poly ADP Ribose Polymerase (PARP). …”
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Widespread genomic/molecular alterations of DNA helicases and their clinical/therapeutic implications across human cancer by Xin Qin, Jing Wang, Xing Wang, Tao Huang, Zhiqing Fang, Lei Yan, Yidong Fan, Dawei Xu

“…High DHSs were also associated with homologous recombination deficiency (HRD), BRCA1/2 mutations and sensitivity to PARP inhibitors. …”
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Platinum-Resistant Ovarian Cancer Is Vulnerable to the cJUN-XRCC4 Pathway Inhibition by Manman Xu, Xi Huang, Cuimiao Zheng, Junming Long, Qingyuan Dai, Yangyang Chen, Jingyi Lu, Chaoyun Pan, Shuzhong Yao, Jie Li

“…Repair of DSB by homologous recombination (HR) and nonhomologous end joining (NHEJ) is frequently associated with platinum resistance in ovarian cancer. …”
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Overexpression of tripartite motif-containing 47 (TRIM47) confers sensitivity to PARP inhibition via ubiquitylation of BRCA1 in triple negative breast cancer cells by Fengen Liu, Binhui Xie, Rong Ye, Yuankang Xie, Baiyin Zhong, Jinrong Zhu, Yao Tang, Zelong Lin, Huiru Tang, Ziqing Wu, Heping Li

“…Abstract Triple-negative breast cancers (TNBC) frequently harbor defects in DNA double-strand break repair through homologous recombination (HR), such as BRCA1 dysfunction. However, less than 15% of TNBC patients were found to carry BRCA1 mutation, indicating that there are other mechanisms regulating BRCA1-deficient in TNBC. …”
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The prognostic significance of BRCA1 gene expression in patients with breast cancer by M. M. Tsyganov, M. K. Ibragimova, A. M. Pevzner, E. Yu. Garbukov, E. M. Slonimskaya, N. V. Litviakov

“…Of particular interest is the assessment of the deficit of homologous recombination, which may include various dysfunctions of the BRCA1 gene. …”
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Construction of a DDR-related signature for predicting of prognosis in metastatic colorectal carcinoma by Maohua Wei, Junyan Su, Jiali Zhang, Siyao Liu, Jia Ma, Xiang peng Meng

“…GSEA functional analysis revealed that the most significantly enriched pathways focused on nucleotide excision repair, base excision repair, homologous recombination, cytokine receptor interaction, chemokine signal pathway, cell adhesion molecules cams, ECM-receptor interaction, and focal adhesion.ConclusionThe DDRscore was identified as an independent prognostic and therapy response predictor, and the DDR-related genes may be potential diagnosis or prognosis biomarkers for mCRC patients.…”
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Sequence Motif Analysis of PRDM9 and Short Inverted Repeats Suggests Their Contribution to Human Microdeletion and Microduplication Syndromes by Paris Ladias, Georgios S. Markopoulos, Charilaos Kostoulas, Ioanna Bouba, Agis Georgiou, Sofia Markoula, Ioannis Georgiou

“…Their prime physiological role is meiotic homologous recombination, resulting in rearrangements of the genetic material. …”
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The C-Terminal Repeat Units of SpaA Mediate Adhesion of <i>Erysipelothrix rhusiopathiae</i> to Host Cells and Regulate Its Virulence by Chao Wu, Zhewen Zhang, Chao Kang, Qiang Zhang, Weifeng Zhu, Yadong Zhang, Hao Zhang, Jingfa Xiao, Meilin Jin

“…To examine whether these deletions or splits influence <i>E. rhusiopathiae</i> virulence, these 120 bp were successfully deleted from the <i>spaA</i> gene in strain SE38 by homologous recombination. The mutant strain <i>ΔspaA</i> displayed attenuated virulence in mice and decreased adhesion to porcine iliac artery endothelial cells, which was also observed using the corresponding mutant protein SpaA’. …”
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A genetic tool to express long fungal biosynthetic genes by Leo Kirchgaessner, Jacob M. Wurlitzer, Paula S. Seibold, Malik Rakhmanov, Markus Gressler

“…The akuB gene was deleted in the expression host A. niger ATNT∆pyrG, resulting in a deficient nonhomologous end-joining repair mechanism which in turn facilitates the targeted gene deletion via homologous recombination. The ∆akuB mutant tLK01 served as a platform to integrate overlapping DNA fragments of long SM genes into the fwnA locus required for the black pigmentation of conidia. …”
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Olaparib-Resistant <i>BRCA2</i>^MUT Ovarian Cancer Cells with Restored BRCA2 Abrogate Olaparib-Induced DNA Damage and G2/M Arrest Controlled by the ATR/CHK1 Pathway for S... by Łukasz Biegała, Arkadiusz Gajek, Agnieszka Marczak, Aneta Rogalska

“…The PARP inhibitor (PARPi) olaparib is currently the drug of choice for serous ovarian cancer (OC), especially in patients with homologous recombination (HR) repair deficiency associated with deleterious <i>BRCA1/2</i> mutations. …”
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Triplet maintenance therapy of olaparib, pembrolizumab and bevacizumab in women with BRCA wild-type, platinum-sensitive recurrent ovarian cancer: the multicenter, single-arm phase... by Yoo-Na Kim, Boram Park, Jae Weon Kim, Byoung Gie Kim, Sang Wun Kim, Hee Seung Kim, Chel Hun Choi, Myong Cheol Lim, Natalie YL Ngoi, David SP Tan, Jung-Yun Lee

“…Exploratory analysis shows that patients who were homologous recombination deficiency-positive or had a programmed death-ligand 1 combined positive score ≥1 showed a favorable response (P = 0.043 and P < 0.001, respectively). …”
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Targeting HR Repair as a Synthetic Lethal Approach to Increase DNA Damage Sensitivity by a RAD52 Inhibitor in BRCA2-Deficient Cancer Cells by Wei-Che Tseng, Chi-Yuan Chen, Ching-Yuh Chern, Chu-An Wang, Wen-Chih Lee, Ying-Chih Chi, Shu-Fang Cheng, Yi-Tsen Kuo, Ya-Chen Chiu, Shih-Ting Tseng, Pei-Ya Lin, Shou-Jhen Liou, Yi-Chen Li, Chin-Chuan Chen

“…More specifically, in MCF7-siBRCA2 cells, curcumin reduced homologous recombination, resulting in tumor growth suppression. …”
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Photonic Crystal Surface Mode Real-Time Imaging of RAD51 DNA Repair Protein Interaction with the ssDNA Substrate by Galina Nifontova, Cathy Charlier, Nizar Ayadi, Fabrice Fleury, Alexander Karaulov, Alyona Sukhanova, Igor Nabiev

“…The RAD51 protein plays a central role in DNA repair via the homologous recombination pathway. This recombinase is essential for the genome stability and its overexpression is often correlated with aggressive cancer. …”
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<i>Trichosporon asahii PLA2</i> Gene Enhances Drug Resistance to Azoles by Improving Drug Efflux and Biofilm Formation by Xiaoping Ma, Hong Liu, Zhen Liu, Ya Wang, Zhijun Zhong, Guangneng Peng, Yu Gu

“…TaPLA2^OE was generated by homologous recombination of the recombinant vector pEGFP-N1-TaPLA2, induced by the CMV promoter, with <i>Agrobacterium tumefaciens</i>. …”
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A recombinant capripoxvirus expressing the F protein of peste des petits ruminants virus and the P12A3C of foot-and-mouth disease virus by Jidong Li, Jianlin Wang, Yanan Guo, Zhenxing Gong, Xuepeng Cai

“…Results The IRES, FMDV P12A3C and PPRV F genes into the multi-cloning site of the universal transfer plasmid pTKfpgigp to construct a recombinant transfer plasmid pTKfpgigpFiP12A3C, and transfected GPV-infected lamb testis (LT) cells with liposomes and produced by homologous recombination Recombinant GPV (rGPV/PPRVF-FMDVP12A3C, rGPV). …”
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Rearrangement and domestication as drivers of Rosaceae mitogenome plasticity by Manyi Sun, Mingyue Zhang, Xuening Chen, Yueyuan Liu, Binbin Liu, Jiaming Li, Runze Wang, Kejiao Zhao, Jun Wu

“…After the DEL-D sequence fragments originally arose, they may have experienced a subsequent doubling event via homologous recombination and sequence transfer in the Amygdaloideae; afterwards, this variant sequence may have significantly expanded to cultivated groups, thereby improving adaptation during the domestication process. …”
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Fanconi Anemia Pathway in Colorectal Cancer: A Novel Opportunity for Diagnosis, Prognosis and Therapy by Fatemeh Ghorbani Parsa, Stefania Nobili, Mina Karimpour, Hamid Asadzadeh Aghdaei, Ehsan Nazemalhosseini-Mojarad, Enrico Mini

“…The FA pathway is a substantial part of the DNA damage response system that participates in the repair of DNA inter-strand crosslinks through homologous recombination (HR) and protects genome stability via replication fork stabilization, respectively. …”
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Secretion of IFN-γ by Transgenic Mammary Epithelial Cells in vitro Reduced Mastitis Infection Risk in Goats by Ying Liu, Hongyan Zhang, Shasha Dong, Boyu Li, Weiming Ma, Lijiang Ge, Zhiyong Hu, Feng Su

“…In this study, we initially constructed targeting and homologous recombination vectors (containing the IFN-γ gene) and then transferred the vectors into goat mammary gland epithelial cells (GMECs). …”
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Inhibition of BAD-Ser99 phosphorylation synergizes with PARP inhibition to ablate PTEN-deficient endometrial carcinoma by Xi Zhang, Peng Huang, Liqiong Wang, Shu Chen, Basappa Basappa, Tao Zhu, Peter E. Lobie, Vijay Pandey

“…Combined NPB-PARPi treatment of PTEN-deficient EC cells stimulated apoptosis and promoted DNA damage by impairment of homologous recombination. Using the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 endonuclease system it was demonstrated that deletion of PTEN in PTEN replete EC cells enhanced the efficacy of combined NPB-PARPi treatment. …”
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Integration Host Factor Binds DNA Holliday Junctions by Shawn H. Lin, Dacheng Zhao, Vivian Deng, Veronica K. Birdsall, Suzanne Ho, Olga Buzovetsky, Candice M. Etson, Ishita Mukerji

“…Junctions, important intermediates in DNA repair and homologous recombination, are dynamic and can adopt either an open or stacked conformation, where the open conformation facilitates branch migration and strand exchange. …”
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