RNAi Screening Uncovers a Synthetic Sick Interaction between CtIP and the BARD1 Tumor Suppressor by Hella A. Bolck, Sara Przetocka, Roger Meier, Christine von Aesch, Christina Zurfluh, Kay Hänggi, Vincent Spegg, Matthias Altmeyer, Michael Stebler, Simon F. Nørrelykke, Peter Horvath, Alessandro A. Sartori, Antonio Porro

“…Human CtIP is best known for its role in DNA end resection to initiate DNA double-strand break repair by homologous recombination. Recently, CtIP has also been shown to protect reversed replication forks from nucleolytic degradation upon DNA replication stress. …”
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Case report: A germline CHEK1 c.613 + 2T>C leads to a splicing error in a family with multiple cancer patients by Jun Qian, Min Peng, Yanan Li, Wei Liu, Xinwei Zou, Huafei Chen, Sujuan Zhou, Sheng Xiao, Jinhua Zhou

“…Despite its well-established role in homologous recombination, germline mutations in CHEK1 are rarely reported.Case presentationIn this report, we present a patient diagnosed with ovarian clear cell carcinoma who has a family history of cancer. …”
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DNA Damage Clustering after Ionizing Radiation and Consequences in the Processing of Chromatin Breaks by Veronika Mladenova, Emil Mladenov, Martin Stuschke, George Iliakis

“…Furthermore, we summarize evidence that after exposure to high-LET IR, error-prone processes outcompete high fidelity homologous recombination (HR) through mechanisms that remain to be elucidated. …”
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Contribution of the Tyrosinase (MoTyr) to Melanin Synthesis, Conidiogenesis, Appressorium Development, and Pathogenicity in <i>Magnaporthe oryzae</i> by Xiaoning Fan, Penghui Zhang, Wajjiha Batool, Chang Liu, Yan Hu, Yi Wei, Zhengquan He, Shi-Hong Zhang

“…The deletion mutant of MoTyr (Δ<i>MoTyr</i>) was obtained by using a homologous recombination approach. Phenotypic analysis showed that conidiophore stalks and conidia formation was significantly reduced in Δ<i>MoTyr</i>. …”
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Complete Genome Sequencing of <i>Leptospira interrogans</i> Isolates from Malaysia Reveals Massive Genome Rearrangement but High Conservation of Virulence-Associated Genes by Siti Roszilawati Ramli, Boyke Bunk, Cathrin Spröer, Robert Geffers, Michael Jarek, Sabin Bhuju, Marga Goris, Sahlawati Mustakim, Frank Pessler

“…Even though there was some shared synteny and collinearity across the six genomes, there was evidence of major genome rearrangement, likely driven by horizontal gene transfer and homologous recombination. Mobile genetic elements were identified in all strains in highly varying numbers, including in the <i>rfb</i> locus, which defines serogroups and contributes to immune escape and pathogenesis. …”
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CRISPR-Cas9 assisted non-homologous end joining genome editing system of Halomonas bluephagenesis for large DNA fragment deletion by Chunyan Liu, Yaxin Yue, Yanfen Xue, Cheng Zhou, Yanhe Ma

“…However, the efficiency of the established CRISPR-Cas-homologous recombination (HR) gene editing tool for large DNA fragments was still relatively low. …”
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Human Fallopian Tube-Derived Organoids with TP53 and RAD51D Mutations Recapitulate an Early Stage High-Grade Serous Ovarian Cancer Phenotype In Vitro by Yilin Dai, Jing Xu, Xiaofeng Gong, Jinsong Wei, Yi Gao, Ranran Chai, Chong Lu, Bing Zhao, Yu Kang

“…RAD51D deletion impaired the homologous recombination (HR) function and induced G2/M phase arrest, whereas concurrent TP53 deletion mitigated G0/G1 phase arrest and boosted DNA replication when combined with RAD51D mutation. …”
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ERCC1 is a potential biomarker for predicting prognosis, immunotherapy, chemotherapy efficacy, and expression validation in HER2 over-expressing breast cancer by Yilun Li, Xiaomei Liao, Li Ma

“…The data was used to perform differential expression analyses between tumor and normal tissues in pan-cancers, immune-related analysis, homologous recombination deficiency (HRD), tumor mutation burden, and microsatellite instability. …”
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Dual PARP and RAD51 Inhibitory Drug Conjugates Show Synergistic and Selective Effects on Breast Cancer Cells by Matthews M. Malka, Julia Eberle, Kathrin Niedermayer, Darius P. Zlotos, Lisa Wiesmüller

“…The genetic principle of synthetic lethality has most successfully been exploited in therapies engaging Poly-ADP-ribose-polymerase (PARP) inhibitors to treat patients with homologous recombination (HR)-defective tumors. In this work, we went a step further following the idea of a local molecular cooperation and designed hybrid compounds <b>M1–M3</b>. …”
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GIP1 and GIP2 Contribute to the Maintenance of Genome Stability at the Nuclear Periphery by Gaurav Singh, Morgane Batzenschlager, Denisa Tomkova, Etienne Herzog, Elise Hoffmann, Guy Houlné, Anne-Catherine Schmit, Alexandre Berr, Marie-Edith Chabouté

“…This was linked to a constitutive activation of the DDR in the gip1gip2 mutant, with more emphasis on the homologous recombination (HR) repair pathway. In addition, we noticed the presence of numerous RAD51 foci which did not colocalize with γ-H2AX foci. …”
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E7449: A dual inhibitor of PARP1/2 and tankyrase1/2 inhibits growth of DNA repair deficient tumors and antagonizes Wnt signaling by Chang, Paul

“…Cells deficient in DNA repair pathways beyond homologous recombination were sensitive to E7449 treatment. …”
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DNA repair capacity in multiple pathways predicts chemoresistance in glioblastoma multiforme by Kitange, Gaspar J., Gupta, Shiv K., Sarkaria, Jann N., Nagel, Zachary D., Joughin, Brian Alan, Chaim, Isaac Alexander, Mazzucato, Patrizia, Lauffenburger, Douglas A, Samson, Leona D

“…This work showed that, in addition to changes in O6-methylguanine DNA methyltransferase (MGMT) activity, small changes in mismatch repair (MMR), nucleotide excision repair (NER), and homologous recombination (HR) capacity contributed to acquired TMZ resistance in PDX models, and lead to reduced relative survival prolongation following TMZ treatment of orthotopic mouse models in vivo. …”
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Scarless Gene Tagging with One-Step Transformation and Two-Step Selection in Saccharomyces cerevisiae and Schizosaccharomyces pombe by Landgraf, Dirk, Huh, Dann, Hallacli, Erinc, Lindquist, Susan

“…The method relies on sequential positive and negative selection and uses an integration cassette with long flanking regions, which is assembled by two-step PCR, to increase the homologous recombination frequency. The method also enables scarless tagging of essential genes with no need for a complementing plasmid. …”
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Multiple mechanisms contribute to double-strand break repair at rereplication forks in Drosophila follicle cells by Beagan, Kelly, McVey, Mitch, Alexander, Jessica Lynne, Orr-Weaver, Terry

“…Conversely, we show that fork progression is enhanced in the absence of both Drosophila Rad51 homologs, spindle-A and spindle-B, revealing homologous recombination is active and actually impairs fork movement during follicle cell rereplication. …”
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Investigation into the role of DNA damage and repair during influenza infection and inflammation by Parrish, Marcus Curtis

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Cdk12 regulates DNA repair Genes by suppressing intronic polyadenylation by Dubbury, Sara Jane

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REV7 Monomer Is Unable to Participate in Double Strand Break Repair and Translesion Synthesis but Suppresses Mitotic Errors by Vassel, Faye M., Laverty, Daniel J., Bian, Ke, Piett, Cortt G., Hemann, Michael T., Walker, Graham C., Nagel, Zachary D.

“…The Rev7 dimerization interface is also required for shieldin function, as both Rev7^{&minus;/&minus;} cells and Rev7^{&minus;/&minus;} cells expressing Rev7^{K44A/R124A/A135D} exhibit decreased proficiency in rejoining some types of double strand breaks, as well as increased homologous recombination. Interestingly, Rev7^{K44A/R124A/A135D} retains some function in cell cycle regulation, as it maintains an interaction with Ras-related nuclear protein (Ran) and partially rescues the formation of micronuclei. …”
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Methyltransferases mediate cell memory of a genotoxic insult by Engelward, Bevin P., Rugo, Rebecca, Mutamba, James Tendai, Yee, Tiffany L., Chaillet, J. R., Greenberger, Joel S., Mohan, K. N.

“…Specifically, conditioned media from the progeny of exposed cells can induce DNA damage and homologous recombination in naive cells. Notably, cells exposed to conditioned media also elicit a genome-destabilizing effect on their neighbouring cells, thus demonstrating transmission of genomic instability. …”
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Irradiated Esophageal Cells are Protected from Radiation-Induced Recombination by MnSOD Gene Therapy by Niu, Yunyun, Wang, Hong, Wiktor-Brown, Dominika, Rugo, Rebecca, Shen, Hongmei, Huq, Saiful, Engelward, Bevin P., Epperley, Michael, Greenberger, Joel S.

“…Specifically, we measured the frequency of homologous recombination (HR) at an integrated transgene in the Fluorescent Yellow Direct Repeat (FYDR) mice, in which an HR event can give rise to a fluorescent signal. …”
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Response of DNA repair and replication systems to exocyclic nucleic acid base damage by Śrīvāstava, Nidhi

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