Graphene-based composites for biomedical applications by Selsabil Rokia Laraba, Wei Luo, Amine Rezzoug, Qurat ul ain Zahra, Shihao Zhang, Bozhen Wu, Wen Chen, Lan Xiao, Yuhao Yang, Jie Wei, Yulin Li

“…Over the last decade, the superior properties of graphene have contributed to intensive studies on the fabrication and applications of graphene nanocomposites. Ex-situ homologous recombination and recombination techniques were listed. …”
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Characterizing PALB2 intragenic duplication breakpoints in a triple-negative breast cancer case using long-read sequencing by Iulian O. Ban, Alice Chabert, Thomas Guignard, Thomas Guignard, Jacques Puechberty, Jacques Puechberty, Simon Cabello-Aguilar, Simon Cabello-Aguilar, Pascal Pujol, Julie A. Vendrell, Jérôme Solassol, Jérôme Solassol

“…Long-read sequencing enabled the localization of breakpoints within Alu elements, providing insights into the mechanism of duplication via non-allelic homologous recombination.ConclusionUsing our combined methodology, we reclassified the PALB2 duplication as a pathogenic variant. …”
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Cell Cycle Stage and DNA Repair Pathway Influence CRISPR/Cas9 Gene Editing Efficiency in Porcine Embryos by Karina Gutierrez, Werner G. Glanzner, Mariana P. de Macedo, Vitor B. Rissi, Naomi Dicks, Rodrigo C. Bohrer, Hernan Baldassarre, Luis B. Agellon, Vilceu Bordignon

“…This study evaluated the importance of zygote cell cycle stage between early-injection (within 6 h post activation/fertilization) versus late-injection (14–16 h post activation/fertilization) when the CRISPR/Cas9 components were injected and the inhibition of the homologous recombination (HR) pathway of DNA repair on gene editing, embryo survival and development on embryos produced by fertilization, sperm injection, somatic cell nuclear transfer, and parthenogenetic activation technologies. …”
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Horizontal Gene Transfer to a Defensive Symbiont with a Reduced Genome in a Multipartite Beetle Microbiome by Samantha C. Waterworth, Laura V. Flórez, Evan R. Rees, Christian Hertweck, Martin Kaltenpoth, Jason C. Kwan

“…Unexpectedly, we uncovered evidence of additional horizontal transfers to the symbiont’s genome while genome reduction was occurring and despite a current lack of genes needed for homologous recombination. These gene gains may have given the genome-reduced symbiont a selective advantage in the microbiome, especially given the maintenance of the large lga gene cluster despite ongoing genome reduction. …”
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Selective optogenetic activation of NaV1.7-expressing afferents in NaV1.7-ChR2 mice induces nocifensive behavior without affecting responses to mechanical and thermal stimuli. by Toyoaki Maruta, Kotaro Hidaka, Satoshi Kouroki, Tomohiro Koshida, Mio Kurogi, Yohko Kage, Seiya Mizuno, Tetsuro Shirasaka, Toshihiko Yanagita, Satoru Takahashi, Ryu Takeya, Isao Tsuneyoshi

“…We used CRISPR/Cas9-mediated homologous recombination to generate bicistronic NaV1.7-iCre knock-in mice, which express iCre recombinase under the endogenous NaV1.7 gene promoter without disrupting NaV1.7. …”
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Chemical Inhibition of RPA by HAMNO Alters Cell Cycle Dynamics by Impeding DNA Replication and G2-to-M Transition but Has Little Effect on the Radiation-Induced DNA Damage Response by Rositsa Dueva, Lisa Marie Krieger, Fanghua Li, Daxian Luo, Huaping Xiao, Martin Stuschke, Eric Metzen, George Iliakis

“…Furthermore, HAMNO increases the fraction of chromatin-bound RPA in S-phase but not in G2-phase cells and suppresses DSB repair by homologous recombination. Despite these marked effects on the cell cycle and the DNA damage response, radiosensitization could neither be detected in exponentially growing cultures, nor in cultures enriched in G2-phase cells. …”
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Knockout of Targeted Plasmid-Borne β-Lactamase Genes in an Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strain: Impact on Resistance and Proteomic Profile by Daniel Jaén-Luchoro, Roger Karlsson, Antonio Busquets, Beatriz Piñeiro-Iglesias, Nahid Karami, Nachiket P. Marathe, Edward R. B. Moore

“…The aim of our study was to develop a system for assessing the phenotypic and proteomic responses of bacteria to antibiotic stress as a result of the loss of selected antimicrobial resistance genes. We applied homologous recombination to knock out plasmid-borne β-lactamase genes (blaOXA-1, blaTEM-1, and blaCTX-M15) in Escherichia coli CCUG 73778, generating knockout clone variants lacking the respective deleted β-lactamases. …”
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Genome Editing of Golden SNP-Carrying Lycopene Epsilon-Cyclase (<i>LcyE</i>) Gene Using the CRSPR-Cas9/HDR and Geminiviral Replicon System in Rice by Jong Hee Kim, Jihyeon Yu, Hee Kyoung Kim, Jin Young Kim, Me-Sun Kim, Yong-Gu Cho, Sangsu Bae, Kwon Kyoo Kang, Yu Jin Jung

“…The phenotype of the homologous recombination (HR) mutant obtained through the geminiviral replicon-based template delivery system was tangerine color, and the frequency was 1.32% of the transformed calli. …”
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Carboplatin in metastatic castration-resistant prostate cancer patients with molecular alterations of the DNA damage repair pathway: the PRO-CARBO phase II trial by Elodie Coquan, Nicolas Penel, Justine Lequesne, Raphaël Leman, Pernelle Lavaud, Zoé Neviere, Pierre-Emmanuel Brachet, Emeline Meriaux, Aurélien Carnot, Jérémy Boutrois, Marie Castera, Nicolas Goardon, Etienne Muller, Alexandra Leconte, Antoine Thiery-Vuillemin, Bénédicte Clarisse, Florence Joly

“…Poly-ADP (Adénosine Diphosphate)-ribose polymerase inhibitors (PARPi) showed significant activity for these selected tumors, especially with homologous recombination repair (HRR) deficiency. These alterations could also predict platinum sensitivity. …”
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Consensus on the management of platinum-sensitive high-grade serous epithelial ovarian cancer in Lebanon by Reem Abdallah, David Atallah, Nizar Bitar, Georges Chahine, Hady Ghanem, Marwan Ghosn, Joseph Kattan, Fadi Nasr, Joseph Makdessi, Ali Shamseddine

“…PARP inhibitor use depends on the patient’s genetic signature, mainly the breast cancer gene (BRCA) mutation and the homologous recombination deficiency (HRD) status. Therefore, genetic testing is recommended at diagnosis to inform treatment and prognosis.In line with the evolving standard-of-care for ovarian cancer, a panel of experts in treating advanced ovarian cancer convened to lay down practical recommendations on the management of advanced ovarian cancer in Lebanon; since the currently applicable guidelines by the Lebanese Ministry of Public Health for cancer treatment have not been updated yet to reflect the treatment paradigm shift brought upon by the development and approval of PARP inhibitors. …”
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Repression of the SUMO-conjugating enzyme UBC9 is associated with lowered double minutes and reduced tumor progression by Yusi Wang, Hongyan Zou, Wei Ji, Min Huang, Benhui You, Nan Sun, Yuandong Qiao, Peng Liu, Lidan Xu, Xuelong Zhang, Mengdi Cai, Ye Kuang, Songbin Fu, Wenjing Sun, Xueyuan Jia, Jie Wu

“…Western blot and immunofluorescence staining were then used to determine DNA damage extent, and a reporter plasmid system was constructed to detect changes in homologous recombination (HR) and non-homologous end joining (NHEJ) pathways. …”
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Identifying liver metastasis-related hub genes in breast cancer and characterizing SPARCL1 as a potential prognostic biomarker by Mingkuan Chen, Wenfang Zheng, Lin Fang

“…GSEA results suggested that low SPARCL1 expression in BC was related to the cell cycle, DNA replication, oxidative phosphorylation, and homologous recombination. Lower expression levels of SPARCL1 were detected in BC tissues compared to adjacent tissues. …”
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PARP inhibitors as first-line maintenance therapy in ovarian cancer: recommendations from an expert panel from Brazil by Aknar FC Calabrich, Daniele Xavier Assad, Graziela Dal-Molin, Andreia C Melo, Angelica Nogueira-Rodrigues, Karime Kalil, Andrea PG Guimarães, Michelle Samora Almeida, Carla Rameri de Azevedo, Daniela de Freitas, Alessandra Menezes Morelle, Ana Carolina Leite, Marcela Crosara, João Soares Nunes, Poliana Signorini, Eduardo Cronemberger, Rachel Cossetti, Rodrigo Guindalini, Eduardo Paulino, Fernando C Maluf

“…The panel reached consensus (at least 75% of votes for the same recommendation) or majority vote (50% to 74.9%) for the majority of questions that addressed: (1) who and when to test for BRCA mutations or homologous recombination deficiency (2) what test should be used; (3) when should maintenance PARP inhibitor therapy be indicated; (4) which PARP inhibitor should be used; (5) when should bevacizumab be combined; and (6) toxicity management. …”
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Proteomic Investigation of the Role of Nucleostemin in Nucleophosmin-Mutated OCI-AML 3 Cell Line by Ilaria Cela, Maria Concetta Cufaro, Maurine Fucito, Damiana Pieragostino, Paola Lanuti, Michele Sallese, Piero Del Boccio, Adele Di Matteo, Nerino Allocati, Vincenzo De Laurenzi, Luca Federici

“…In particular, we report an effect of down-regulating DNA repair through homologous recombination, and we confirmed a higher DNA damage rate in OCI-AML 3 cells when NS is depleted, which considerably increases upon stress induced by the topoisomerase II inhibitor etoposide. …”
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Emerging and re-emerging KPC-producing hypervirulent Pseudomonas aeruginosa ST697 and ST463 between 2010 and 2021 by Biying Zhang, Xun Xu, Xiaomei Song, Yicheng Wen, Zhichen Zhu, Jingnan Lv, Xiaofang Xie, Liang Chen, Yi-Wei Tang, Hong Du

“…The complete sequencing of ST697 isolate SE5416 showed that the harbouring of blaKPC-2 resulted from complex transposition and homologous recombination of an IncpRBL16 plasmid and other mobile elements. …”
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Stn1-Ten1 and Taz1 independently promote replication of subtelomeric fragile sequences in fission yeast by Mélina Vaurs, Karel Naiman, Chaïnez Bouabboune, Sudhir Rai, Katarzyna Ptasińska, Marion Rives, Samah Matmati, Antony M. Carr, Vincent Géli, Stéphane Coulon

“…We further show that, when ST function is compromised, a homologous recombination (HR)-based fork restart mechanism becomes necessary for STE3-2 stability. …”
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ECPPF (E2F1, CCNA2, POLE, PPP2R1A, FBXW7) stratification: Profiling high-risk subtypes of histomorphologically low-risk and treatment-insensitive endometrioid endometrial cancer. by Jesus Gonzalez-Bosquet, S John Weroha, Jamie N Bakkum-Gamez, Amy L Weaver, Michaela E McGree, Sean C Dowdy, Abimbola O Famuyide, Benjamin R Kipp, Kevin C Halling, Siddhartha Yadav, Fergus J Couch, Karl C Podratz

“…Expression of homologous recombination repair (HR) and cell cycle genes was significantly elevated in CCNA2-H/E2F1-H compared with CCNA2-L/E2F1-L (P<1.0E-10), suggesting that HR deficiencies may underlie the favorable PFS in MLR. …”
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Lysine Acetylome Profiling Reveals Diverse Functions of Acetylation in Deinococcus radiodurans by Yongqian Zhang, Nuomin Li, Qiushi Wei, Rui Min, Feng Liu, Fuli Wang, Yulin Deng

“…Protein-protein interaction networks demonstrate that four clusters are involved in DNA damage repair, including homologous recombination, mismatch repair, nucleotide excision repair, and base excision repair, which suggests that acetylation plays an indispensable role in the extraordinary capacity to survive high levels of ionizing radiation. …”
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Understanding the research advances on lumpy skin disease: A comprehensive literature review of experimental evidence by Zhengji Liang, Kaishen Yao, Shasha Wang, Juanbin Yin, Xiaoqin Ma, Xiangping Yin, Xiangwei Wang, Yuefeng Sun

“…In addition, because LSDV has a huge genome, it is currently also used as a vaccine carrier, forming a new complex with other viral genes through homologous recombination. The vaccine prepared based on this can have a certain preventive effect on many kinds of diseases. …”
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Comprehensive Analysis of Genomic Alterations in Hepatoid Adenocarcinoma of the Stomach and Identification of Clinically Actionable Alterations by Rongjie Zhao, Hongshen Li, Weiting Ge, Xiuming Zhu, Liang Zhu, Xiangbo Wan, Guanglan Wang, Hongming Pan, Jie Lu, Weidong Han

“…Mutagenesis mechanisms in HAS were associated with spontaneous or enzymatic deamination of 5-methylcytosine to thymine and defective homologous recombination-related DNA damage repair. However, LIHC was characteristic of exposure to aflatoxin and aristolochic acid. …”
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