The reaction mechanism of the Ideonella sakaiensis PETase enzyme by Tucker Burgin, Benjamin C. Pollard, Brandon C. Knott, Heather B. Mayes, Michael F. Crowley, John E. McGeehan, Gregg T. Beckham, H. Lee Woodcock

“…Abstract Polyethylene terephthalate (PET), the most abundantly produced polyester plastic, can be depolymerized by the Ideonella sakaiensis PETase enzyme. Based on multiple PETase crystal structures, the reaction has been proposed to proceed via a two-step serine hydrolase mechanism mediated by a serine-histidine-aspartate catalytic triad. …”
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Degradation of PET Bottles by an Engineered <i>Ideonella sakaiensis</i> PETase by Maria Eduarda Sevilla, Mario D. Garcia, Yunierkis Perez-Castillo, Vinicio Armijos-Jaramillo, Santiago Casado, Karla Vizuete, Alexis Debut, Liliana Cerda-Mejía

“…In recent years, enzymatic degradation of PET by <i>Ideonella sakaiensis</i> PETase (<i>Is</i>PETase), a cutinase-like enzyme, has emerged as a promising strategy to completely depolymerize this polymer into its building blocks. …”
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Plastic Filtration and Decomposition According to Ricochet Filtering Mechanism Using Ideonella sakaiensis by Prakhar Sankrityayan, Sumit Biswas

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<i>Ideonella sakaiensis</i> Can Metabolize Bisphenol A as a Carbon Source by Cristian-Emilian Pop, György Deák, Cristina Maria, Gina Ghiță, Alexandru Anton Ivanov, Sergiu Fendrihan, Dan Florin Mihăilescu, Maria Mernea

Subjects: “…<i>Ideonella sakaiensis</i>…”
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Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification by Binyang Deng, Yu Yue, Jun Yang, Mingjun Yang, Qiong Xing, Hang Peng, Fei Wang, Ming Li, Lixin Ma, Chao Zhai

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Analysis of the plastic-degrading bacteria utilization by Choi, Donghyun

“…It was demonstrated that the whole microbial population could convert 75% of the decomposed polyethylene terephthalate (PET) into carbon dioxide after it had been initially broken down and absorbed by Ideonella sakaiensis. This report will use Ideonella sakaiensis as a control and compare other bacteria growing on PET in a condition where nutrients are fed sufficiently. …”
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Plastics Biodegradation: The Situation Now and Its Potential Effects on Environmental Safety by F. A. Igiebor, E. M. Jonathan, O. Haruna, B. I. Alenkhe

“…In the absence of appropriate waste management and litter control techniques, the use of biodegradable plastics for specialised applications is a promising idea. Ideonella sakaiensis 201-F6 is a brand-new bacterial strain that has been discovered to be capable of breaking down PET. …”
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Cationic and Non-ionic Surfactant Pre-treatment of Pet to Enhance the Bacterial Biodegradation of Pet by Seyedehazita Ahmaditabatabaei, Hafiz M.N. Iqbal, Zeynab Moinipoor, Godfrey Kyazze, Tajalli Keshavarz

“…DTAB treatment in the presence of the bacterial culture Ideonella sakaiensis (I. sakaiensis) in Yeast Extract-Sodium Carbonate and Vitamins (YSV) medium depicted notable potential for enhancement of the degradation of PET. …”
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A minireview on the bioremediative potential of microbial enzymes as solution to emerging microplastic pollution by Rener De Jesus, Ruwaya Alkendi

“…Identified microbial enzymes, such as IsPETase and IsMHETase from Ideonella sakaiensis 201-F6 and Thermobifida fusca cutinase (Tfc), are able to depolymerize plastic polymer chains producing ecologically harmless molecules like carbon dioxide and water. …”
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Rational Protein Engineering to Increase the Activity and Stability of <i>Is</i>PETase Using the PROSS Algorithm by Andrew Rennison, Jakob R. Winther, Cristiano Varrone

“…Here, <i>Is</i>PETase, derived from the bacterium <i>Ideonella sakaiensis</i>, is considered to be the most active enzyme for PET degradation under mild conditions, and although several studies have demonstrated improvements to both the stability and activity of this enzyme, stability at even moderate temperatures is still an issue. …”
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An absorbance method for analysis of enzymatic degradation kinetics of poly(ethylene terephthalate) films by Zhong-Johnson, En Ze Linda, Voigt, Christopher A, Sinskey, Anthony J

“…A thermostable variant of PETase from Ideonella sakaiensis was engineered (R280A S121E D186H N233C S282C) with a denaturation temperature of 69.4 ± 0.3 °C. …”
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A PETase enzyme synthesised in the chloroplast of the microalga Chlamydomonas reinhardtii is active against post-consumer plastics by Giulia Di Rocco, Henry N. Taunt, Marcello Berto, Harry O. Jackson, Daniele Piccinini, Alan Carletti, Giulia Scurani, Niccolò Braidi, Saul Purton

“…A photosynthetic-restoration strategy was used to generate a marker-free transformant line of the green microalga Chlamydomonas reinhardtii in which the PETase from Ideonella sakaiensis was constitutively expressed in the chloroplast. …”
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Impact of Enzymatic Degradation on the Material Properties of Poly(Ethylene Terephthalate) by Teresa Menzel, Sebastian Weigert, Andreas Gagsteiger, Yannik Eich, Sebastian Sittl, Georg Papastavrou, Holger Ruckdäschel, Volker Altstädt, Birte Höcker

“…This study focuses on recycled, bottle-grade poly(ethylene terephthalate) (r-PET) and the degrading enzyme PETase from <i>Ideonella sakaiensis</i>. Compact tension (CT) specimens were incubated in an enzymatic solution and thermally and mechanically characterized. …”
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Surface Interaction of Ionic Liquids: Stabilization of Polyethylene Terephthalate-Degrading Enzymes in Solution by Zeenat Zara, Deepti Mishra, Saurabh Kumar Pandey, Eva Csefalvay, Fatemeh Fadaei, Babak Minofar, David Řeha

“…The effect of aqueous solutions of selected ionic liquids solutions on <i>Ideonella sakaiensis</i> PETase with bis(2-hydroxyethyl) terephthalate (BHET) substrate were studied by means of molecular dynamics simulations in order to identify the possible effect of ionic liquids on the structure and dynamics of enzymatic Polyethylene terephthalate (PET) hydrolysis. …”
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Current advances in the structural biology and molecular engineering of PETase by Fei Liu, Tao Wang, Wentao Yang, Yingkang Zhang, Yuming Gong, Xinxin Fan, Guocheng Wang, Zhenhua Lu, Jianmin Wang

“…Recently, a novel mesophilic PET-degrading enzyme (IsPETase) from Ideonella sakaiensis was found to streamline PET biodegradation at 30°C, albeit with a lower PET-degrading activity than chitinase or chitinase-like PET-degrading enzymes. …”
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Assessment of <i>Is</i>PETase-Assisted Depolymerization of Terephthalate Aromatic Polyesters and the Effect of the Thioredoxin Fusion Domain by Paula Wagner-Egea, Virginia Tosi, Ping Wang, Carl Grey, Baozhong Zhang, Javier A. Linares-Pastén

“…In the present work, we compared the <i>Is</i>PETase (from <i>Ideonella sakaiensis</i> 201-F6) activity on a series of polyesters, including poly(butylene) terephthalate (PBT), poly(hexamethylene) terephthalate (PHT) and Akestra™, with PET. …”
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Biochemical characterization of a polyethylene terephthalate hydrolase and design of high-throughput screening for its directed evolution by Xiaotong Wang, Chaoyi Song, Qingsheng Qi, Youming Zhang, Ruijuan Li, Liujie Huo

“…In this work, PET hydrolase SbPETase from Schlegelella brevitalea sp. nov. was biochemically characterized, and rational design was performed based on its sequence similarity with the previously reported IsPETase from Ideonella sakaiensis, resulting in a triple mutant with increased activity. …”
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Development of a yeast whole-cell biocatalyst for MHET conversion into terephthalic acid and ethylene glycol by Raphael Loll-Krippleber, Victoria A. Sajtovich, Michael W. Ferguson, Brandon Ho, Andrew R. Burns, Brandon J. Payliss, Joseph Bellissimo, Sydney Peters, Peter J. Roy, Haley D. M. Wyatt, Grant W. Brown

“…In particular, the enzymes PETase and MHETase from Ideonella sakaiensis depolymerize PET into the two building blocks used for its synthesis, ethylene glycol (EG) and terephthalic acid (TPA). …”
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Marine PET Hydrolase (PET2): Assessment of Terephthalate- and Indole-Based Polyester Depolymerization by Paula Wagner-Egea, Lucía Aristizábal-Lanza, Cecilia Tullberg, Ping Wang, Katja Bernfur, Carl Grey, Baozhong Zhang, Javier A. Linares-Pastén

“…Compared with <i>Is</i>PETase, an enzyme derived from <i>Ideonella sakaiensis</i>, PET2 showed a lower PET depolymerization yield. …”
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Functional expression of polyethylene terephthalate-degrading enzyme (PETase) in green microalgae by Ji Won Kim, Su-Bin Park, Quynh-Giao Tran, Dae-Hyun Cho, Dong-Yun Choi, Yong Jae Lee, Hee-Sik Kim

“…As a result, a novel plastic degradation enzyme, PETase, which can hydrolyze PET, was discovered in Ideonella sakaiensis 201-F6 in 2016. Results A green algae, Chlamydomonas reinhardtii, which produces PETase, was developed for this study. …”
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