Novel homozygous variants in TTC12 cause male infertility with asthenoteratozoospermia owing to dynein arm complex and mitochondrial sheath defects in flagella by Lanlan Meng, Lanlan Meng, Qiang Liu, Qiang Liu, Chen Tan, Xilin Xu, Xilin Xu, Wenbin He, Wenbin He, Tongyao Hu, Chaofeng Tu, Chaofeng Tu, Yong Li, Juan Du, Juan Du, Qianjun Zhang, Qianjun Zhang, Qianjun Zhang, Guangxiu Lu, Guangxiu Lu, Guangxiu Lu, Li-Qing Fan, Li-Qing Fan, Li-Qing Fan, Ge Lin, Ge Lin, Ge Lin, Hongchuan Nie, Huan Zhang, Huan Zhang, Yue-Qiu Tan, Yue-Qiu Tan, Yue-Qiu Tan

“…The effects of the identified variants were evaluated by in silico analysis, and confirmed by in vitro experiments. Intracytoplasmic sperm injection (ICSI) was used to evaluate the efficiency of assisted reproduction technique therapy.Results and Discussion: Novel homozygous TTC12 variants (c.1467_1467delG (p.Asp490Thrfs*14), c.1139_1139delA (p.His380Profs*4), and c.1117G>A (p.Gly373Arg)) were identified in three (0.96%) of the 314 cases. …”
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Novel HYDIN variants associated with male infertility in two Chinese families by Hui Yu, Hui Yu, Xiao Shi, Zhongmei Shao, Zhongmei Shao, Hao Geng, Hao Geng, Hao Geng, Senzhao Guo, Senzhao Guo, Senzhao Guo, Kuokuo Li, Kuokuo Li, Kuokuo Li, Meng Gu, Meng Gu, Meng Gu, Chuan Xu, Chuan Xu, Chuan Xu, Yang Gao, Yang Gao, Yang Gao, Qing Tan, Qing Tan, Zongliu Duan, Zongliu Duan, Zongliu Duan, Huan Wu, Huan Wu, Huan Wu, Rong Hua, Rong Hua, Rui Guo, Rui Guo, Zhaolian Wei, Zhaolian Wei, Zhaolian Wei, Ping Zhou, Ping Zhou, Ping Zhou, Yunxia Cao, Yunxia Cao, Yunxia Cao, Xiaojin He, Xiaojin He, Xiaojin He, Liang Li, Xiaoping Zhang, Mingrong Lv, Mingrong Lv, Mingrong Lv, Mingrong Lv

“…Compared to the fertile control, HYDIN levels, acrosome and centrosome markers (ACTL7A, ACROSIN, PLCζ1, and Centrin1), and flagella components (TOMM20, SEPT4, SPEF2, SPAG6, and RSPHs) were significantly reduced in HYDIN-deficient patients. Using intracytoplasmic sperm injection (ICSI), the patient successfully achieved clinical pregnancy. …”
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Genetics of Azoospermia: Current Knowledge, Clinical Implications, and Future Directions. Part II: Y Chromosome Microdeletions by Farhat Farrokhi, Hossein Sadeghi-Nejad

“…However, they will transmit the deletions to their male offspring by intracytoplasmic sperm injection.</p>…”
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Trends in Global Assisted Reproductive Technologies Research: a Scientometrics study by Maryam Okhovati, Morteza Zare, Fatemeh Zare, Maliheh Sadat Bazrafshan, Azam Bazrafshan

“…Methods: In March 2015, the MEDLINE database was searched for research publications indexed under ‘reproductive techniques, assisted’ (including the following MeSH headings: in vitro fertilization [IVF]; intracytoplasmic sperm injections; cryopreservation; and ovulation induction), with the following expressions in the fields of title or abstract: intrauterine insemination; sperm donation; embryo/egg donation and surrogate mothers. …”
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Microfluidic Systems for Isolation of Spermatozoa from Testicular Specimens of Non-Obstructive Azoospermic Men: Does/Can It Improve Sperm Yield? by Gary D. Smith, Clementina Cantatore, Dana A. Ohl

“…Intracytoplasmic sperm injection (ICSI) has allowed reproduction options through assisted reproductive technologies (ARTs) for men with no spermatozoa within the ejaculate (azoospermia). …”
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The Association between Embryo Development and Chromosomal Results from PGT-A in Women of Advanced Age: A Prospective Cohort Study by Pornchanit Santamonkunrot, Sonsiri Samutchinda, Pornsri Niransuk, Chonthicha Satirapod, Matchuporn Sukprasert

“…Embryos from women aged 35 years and above who underwent intracytoplasmic sperm injections and PGT-A were studied. Day-3 cleavage-stage embryos were evaluated for their cell number, and day-5 blastocyst-stage embryos were evaluated for their morphological grade. …”
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Core Histones Are Constituents of the Perinuclear Theca of Murid Spermatozoa: An Assessment of Their Synthesis and Assembly during Spermiogenesis and Function after Gametic Fusion by Lauren E. Hamilton, Morgan Lion, Luis Aguila, João Suzuki, Genevieve Acteau, Nicole Protopapas, Wei Xu, Peter Sutovsky, Mark Baker, Richard Oko

“…Employing cell fractionation, immunodetection, mass spectrometry, qPCR, and intracytoplasmic sperm injections (ICSI), we examined the localization, developmental origin, and functional potential of histones from the murid PT. …”
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Cryopreservation of fluorescence activated cell sorted boar spermatozoa based on extracellular ubiquitination by A. Petelák, V. Krylov

“…The sorting rate was sufficient for the preparation of up to 20 spermatozoa aliquots for intracytoplasmic sperm injections (ICSI). Two significantly different groups with good freezability were prepared and stored in LN (0.73% contamination of spermatozoa with high eUb level in non-ubiquitinated group and reversely 6.65% spermatozoa without eUb in highly ubiquitinated group). …”
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EIF4G1 is a novel candidate gene associated with severe asthenozoospermia by Yanwei Sha, Wensheng Liu, Xianjing Huang, Yang Li, Zhiyong Ji, Libin Mei, Shaobin Lin, Shuangbo Kong, Jinhua Lu, Lingyuan Kong, Xingshen Zhu, Zhongxian Lu, Lu Ding

“…This infertility associated with this condition was overcome by intracytoplasmic sperm injections, as his wife became pregnant successfully. …”
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