PEGylated Serp-1 Markedly Reduces Pristane-Induced Experimental Diffuse Alveolar Hemorrhage, Altering uPAR Distribution, and Macrophage Invasion by Qiuyun Guo, Qiuyun Guo, Jordan R. Yaron, John W. Wallen, Kyle F. Browder, Ryan Boyd, Tien L. Olson, Michelle Burgin, Peaches Ulrich, Emily Aliskevich, Lauren N. Schutz, Petra Fromme, Liqiang Zhang, Alexandra R. Lucas

“…Mice were treated with 100 ng/g bodyweight of either Serp-1 as native 55 kDa secreted glycoprotein, or as Serp-1m5, or saline controls after inducing DAH. …”
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Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme by Zhening Zhang, Wenguang G Liang, Lucas J Bailey, Yong Zi Tan, Hui Wei, Andrew Wang, Mara Farcasanu, Virgil A Woods, Lauren A McCord, David Lee, Weifeng Shang, Rebecca Deprez-Poulain, Benoit Deprez, David R Liu, Akiko Koide, Shohei Koide, Anthony A Kossiakoff, Sheng Li, Bridget Carragher, Clinton S Potter, Wei-Jen Tang

“…We used cryoEM to understand both the apo- and insulin-bound dimeric IDE states, revealing that IDE displays a large opening between the homologous ~55 kDa N- and C-terminal halves to allow selective substrate capture based on size and charge complementarity. …”
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Neonatal mice lacking functional Fas death receptors are resistant to hypoxic–ischemic brain injury by Ernest M. Graham, R.Ann Sheldon, Debra L. Flock, Donna M. Ferriero, Lee J. Martin, Declan P. O'Riordan, Frances J. Northington

“…The selective vulnerability of the hippocampus to injury correlates with (1) lower basal expression of [Fas-associated death-domain-like IL-1β-converting enzyme]-inhibitory protein (FLIP), (2) increased degradation of spectrin to its 145 or 150 kDa breakdown product, and (3) a higher percentage of non-apoptotic cell death following neonatal HI. …”
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Molecular Cloning and Temporal Expression Pattern of hsp70 Gene During the Early Life Stages of Seriola aureovittata by Lu FANG, Yongjiang XU, Aijun CUI, Yan JIANG, Bin WANG, Heting ZHOU, Xuezhou LIU

“…A 639 amino acids protein with a molecular weight of 70.1 kDa is encoded by hsp70. The hsp70 spatial expression exhibited a sex dimorphism pattern, with significantly high expression levels in the gill, heart, spleen, and ovary in females, whereas in males, the significantly high expression levels were found in the pituitary, gill, head kidney, and testis. …”
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Nardilysin-regulated scission mechanism activates polo-like kinase 3 to suppress the development of pancreatic cancer by Jie Fu, Jianhua Ling, Ching-Fei Li, Chi-Lin Tsai, Wenjuan Yin, Junwei Hou, Ping Chen, Yu Cao, Ya’an Kang, Yichen Sun, Xianghou Xia, Zhou Jiang, Kenei Furukawa, Yu Lu, Min Wu, Qian Huang, Jun Yao, David H. Hawke, Bih-Fang Pan, Jun Zhao, Jiaxing Huang, Huamin Wang, E. I. Mustapha Bahassi, Peter J. Stambrook, Peng Huang, Jason B. Fleming, Anirban Maitra, John A. Tainer, Mien-Chie Hung, Chunru Lin, Paul J. Chiao

“…Here, we find that development of apoptotic resistance and metastasis of Kras G12D -driven PDAC in mice is accelerated by deleting Plk3, explaining the often-reduced Plk3 expression in human PDAC. Importantly, a 41-kDa Plk3 (p41Plk3) that contains the entire kinase domain at the N-terminus (1-353 aa) is activated by scission of the precursor p72Plk3 at Arg354 by metalloendopeptidase nardilysin (NRDC), and the resulting p32Plk3 C-terminal Polo-box domain (PBD) is removed by proteasome degradation, preventing the inhibition of p41Plk3 by PBD. …”
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APOE ε4 gene dose effect on imaging and blood biomarkers of neuroinflammation and beta-amyloid in cognitively unimpaired elderly by Anniina Snellman, Laura L. Ekblad, Jouni Tuisku, Mikko Koivumäki, Nicholas J. Ashton, Juan Lantero-Rodriguez, Thomas K. Karikari, Semi Helin, Marco Bucci, Eliisa Löyttyniemi, Riitta Parkkola, Mira Karrasch, Michael Schöll, Henrik Zetterberg, Kaj Blennow, Juha O. Rinne

“…The participants underwent 11C-PK11195 PET (targeting 18-kDa translocator protein, TSPO), 11C-PiB PET (targeting Aβ), brain MRI, and neuropsychological testing including a preclinical cognitive composite (APCC). 11C-PK11195 distribution volume ratios and 11C-PiB standardized uptake value ratios (SUVRs) were calculated for regions typical for early Aβ accumulation in AD. …”
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Purification, Biochemical and Kinetic Characterization of a Novel Alkaline <i>sn</i>-1,3-Regioselective Triacylglycerol Lipase from <i>Penicillium</i><i>crustosum</i> Thom Strain P... by Ismail Hasnaoui, Ahlem Dab, Sondes Mechri, Houssam Abouloifa, Ennouamane Saalaoui, Bassem Jaouadi, Alexandre Noiriel, Abdeslam Asehraou, Abdelkarim Abousalham

“…The purified PCrL had a molecular mass of 28 kDa, estimated by SDS-PAGE. The 20 NH₂-terminal amino-acid residues showed a high degree of homology with those of other <i>Penicillium</i> lipases. …”
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Molecular Architecture of the Antiophidic Protein DM64 and its Binding Specificity to Myotoxin II From Bothrops asper Venom by Barbara S. Soares, Surza Lucia G. Rocha, Viviane A. Bastos, Diogo B. Lima, Paulo C. Carvalho, Fabio C. Gozzo, Borries Demeler, Borries Demeler, Borries Demeler, Tayler L. Williams, Janelle Arnold, Amy Henrickson, Thomas J. D. Jørgensen, Tatiana A. C. B. Souza, Jonas Perales, Richard H. Valente, Bruno Lomonte, Francisco Gomes-Neto, Ana Gisele C. Neves-Ferreira

“…DM64 is a toxin-neutralizing serum glycoprotein isolated from Didelphis aurita, an ophiophagous marsupial naturally resistant to snake envenomation. This 64 kDa antitoxin targets myotoxic phospholipases A2, which account for most local tissue damage of viperid snakebites. …”
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Galactosylceramidase deficiency and pathological abnormalities in cerebral white matter of Krabbe disease by Diego Iacono, Shunsuke Koga, Hui Peng, Arulmani Manavalan, Jessica Daiker, Monica Castanedes-Casey, Nicholas B. Martin, Aimee R. Herdt, Michael H. Gelb, Dennis W. Dickson, Chris W. Lee

“…Mature GALC could also be quantitatively detected as a 26 kDa band by western blotting and correlated to enzyme activity in brain tissues. …”
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Sulfated Polysaccharide Extracted from the Green Algae <i>Codium bernabei</i>: Physicochemical Characterization and Antioxidant, Anticoagulant and Antitumor Activity by Fabian A. Figueroa, Roberto T. Abdala-Díaz, Claudia Pérez, Virginia Casas-Arrojo, Aleksandra Nesic, Cecilia Tapia, Carla Durán, Oscar Valdes, Carolina Parra, Gastón Bravo-Arrepol, Luis Soto, José Becerra, Gustavo Cabrera-Barjas

“…The molecular weight of the polysaccharide samples was lower than 20 kDa. Both polysaccharides were thermally stable (Tonset > 190 °C) and showed antioxidant activity according to the ABTS^•+ and DPPH tests, where TPs fractions had higher scavenging activity (35%) compared to the APs fractions. …”
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Detection of Proximal Tubule Involvement by BK Polyomavirus in Kidney Transplant Recipients With Urinary Sediment Double-Immunostaining by Yang Huang, Xu-Tao Chen, Shi-Cong Yang, Hui-Fei Yang, Xiao-Tao Hou, Wen-Fang Chen, Jun Li, Rong-Hai Deng, Jin-Quan Luo, Jin-Yuan Wang, Xue Shen, Li-Zhong Chen, Chang-Xi Wang, Jiang Qiu, Gang Huang, Gang Huang, Gang Huang

“…This study was designed to evaluate the value of urinary sediment double-immunostaining for predicting BKPyV infection in proximal tubular epithelium.Materials and methodsA total of 76 urine sediment cell blocks, as well as the corresponding transplanted kidney tissues with BK polyomavirus associated-nephropathy (BKPyVAN), were evaluated by automatic double-immunostaining with anti-58-kDa Golgi protein (58K, a proximal renal tubular marker) + anti-SV40-T and anti-homogentisate 1, 2-dioxygenase (HGD, a renal tubular marker) + anti-SV40-T.ResultsImmunohistochemical staining demonstrated that 58K was expressed in proximal tubular epithelium but not in distal tubular epithelium or transitional epithelium. …”
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Optimization of Antioxidant Peptides Production from Tryptic Hydrolysis of Pomegranate Seed Protein by Maryam Rahimipanah, Alireza Sadeghi Mahoonak, Mohammad Ghorbani, Hoda Shahiritabarestani, Mohsen Nabimeybodi

“…As long as the protein structure is intact, its amino acid sequence is inactive; however, during proteolysis, fermentation, and gastrointestinal digestion, these amino acids are released as oligopeptides ordinally with less than 20 amino acids and below 10 kDa in molecular weight. These peptides are more digestible and can exhibit specific bioactive properties such antioxidant properties. …”
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Comparative Transcriptomic and Proteomic Analysis of Exopalaemon carinicauda in Response to Alkalinity Stress by Zhen Qin, Zhen Qin, Qianqian Ge, Jiajia Wang, Jiajia Wang, Mingdong Li, Ping Liu, Ping Liu, Jian Li, Jian Li, Jitao Li, Jitao Li

“…There were significantly up-regulated expressions of ion transport related genes including aquaporin, carbonic anhydrase, ammonium transporter Rh type A-like, Na+/H+-exchanger, etc., as well as ion transport proteins including V-type proton ATPase 116 kDa subunit a-like isoform X1, sodium-potassium ATPase beta, vesicle associated membrane protein, etc. after alkalinity exposure, which indicating their important roles in response to alkalinity stress. …”
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Central Ceramide-Induced Hypothalamic Lipotoxicity and ER Stress Regulate Energy Balance by Cristina Contreras, Ismael González-García, Noelia Martínez-Sánchez, Patricia Seoane-Collazo, Jordi Jacas, Donald A. Morgan, Dolors Serra, Rosalía Gallego, Francisco Gonzalez, Núria Casals, Rubén Nogueiras, Kamal Rahmouni, Carlos Diéguez, Miguel López

“…Genetic overexpression of the chaperone GRP78/BiP (glucose-regulated protein 78 kDa/binding immunoglobulin protein) in the ventromedial nucleus of the hypothalamus (VMH) abolishes ceramide action by reducing hypothalamic ER stress and increasing BAT thermogenesis, which leads to weight loss and improved glucose homeostasis. …”
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Full-length recombinant antibodies from Escherichia coli: production, characterization, effector function (Fc) engineering, and clinical evaluation by Md Harunur Rashid

“…This review summarizes recent research, preclinical advancements, and clinical development of E. coli-produced aglycosylated therapeutic antibodies as monoclonal, bispecific, and antibody-drug conjugates for use in autoimmune, oncology, and immuno-oncology areas.Abbreviations: ADA Anti-drug antibody; ADCC Antibody-dependent cellular cytotoxicity; ADCP Antibody-dependent cellular phagocytosis; ADC Antibody-drug conjugate; aFc Aglycosylated Fc; AMD Age-related macular degeneration aTTP Acquired thrombotic thrombocytopenic purpura; BCMA B-cell maturation antigen; BLA Biologics license application; BsAb Bispecific antibody; C1q Complement protein C1q; CDC Complement-dependent cytotoxicity; CDCC Complement-dependent cellular cytotoxicity; CDCP Complement-dependent cellular phagocytosis; CEX Cation exchange chromatography; CFPS Cell-free protein expression; CHO Chinese Hamster Ovary; CH1-3 Constant heavy chain 1-3; CL Constant light chain; DLBCL Diffuse large B-cell lymphoma; DAR Drug antibody ratio; DC Dendritic cell; dsFv Disulfide-stabilized Fv; EU European Union; EGFR Epidermal growth factor receptor; E. coli Escherichia coli; EpCAM Epithelial cell adhesion molecule; Fab Fragment antigen binding; FACS Fluorescence activated cell sorting; Fc Fragment crystallizable; FcRn Neonatal Fc receptor; FcɣRs Fc gamma receptors; FDA Food and Drug Administration; FL-IgG Full-length immunoglobulin; Fv Fragment variable; FolRαa Folate receptor alpha; gFc Glycosylated Fc; GM-CSF Granulocyte macrophage-colony stimulating factor; GPx7 Human peroxidase 7; HCL Hairy cell leukemia; HIV Human immunodeficiency virusl; HER2 Human epidermal growth factor receptor 2; HGF Hepatocyte growth factor; HIC Hydrophobic interaction chromatography; HLA Human leukocyte antigen; IBs Inclusion bodies; IgG1-4 Immunoglobulin 1-4; IP Intraperitoneal; ITC Isothermal titration calorimetry; ITP Immune thrombocytopenia; IV Intravenous; kDa Kilodalton; KiH Knob-into-Hole; mAb Monoclonal antibody; MAC Membrane-attack complex; mCRC Metastatic colorectal cancer; MM Multipl myeloma; MOA Mechanism of action; MS Mass spectrometry; MUC1 Mucin 1; MG Myasthenia gravis; NB Nanobody; NK Natural killer; nsAA Nonstandard amino acid; NSCLC Non-small cell lung cancer; P. aeruginosa Pseudomonas aeruginosa; PD-1 Programmed cell death 1; PD-L1 Programmed cell death-ligand 1; PDI Protein disulfide isomerase; PECS Periplasmic expression cytometric screening; PK Pharmacokinetics; P. pastoris Pichia pastoris; PTM Post-translational modification; Rg Radius of gyration; RA Rheumatoid arthritis; RT-PCR Reverse transcription polymerase chain reaction; SAXS Small angle X-ray scattering; scF Single chain Fv; SCLC Small cell lung cancer; SDS-PAGE Sodium dodecyl sulfate–polyacrylamide gel electrophoresis; SEC Size exclusion chromatography; SEED Strand-exchange engineered domain; sRNA Small regulatory RNA; SRP Signal recognition particle; T1/2 Half-life; Tagg Aggregation temperature; TCR T cell receptor; TDB T cell-dependent bispecific; TF Tissue factor; TIR Translation initiation region; Tm Melting temperature; TNBC Triple-negative breast cancer; TNF Tumor necrosis factor; TPO Thrombopoietin; VEGF Vascular endothelial growth factor; vH Variable heavy chain; vL Variable light chain; vWF von Willebrand factor; WT Wild type…”
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Gene Cloning, Recombinant Expression, Characterization, and Molecular Modeling of the Glycolytic Enzyme Triosephosphate Isomerase from <i>Fusarium oxysporum</i> by Beatriz Hernández-Ochoa, Saúl Gómez-Manzo, Erick Alcaraz-Carmona, Hugo Serrano-Posada, Sara Centeno-Leija, Roberto Arreguin-Espinosa, Miguel Cuevas-Cruz, Abigail González-Valdez, José Alberto Mendoza-Espinoza, Marcelo Acosta Ramos, Leyda Cortés-Maldonado, Alba Mónica Montiel-González, Verónica Pérez de la Cruz, Luz María Rocha-Ramírez, Jaime Marcial-Quino, Edgar Sierra-Palacios

“…The recombinant protein named FoxTPI was purified (97% purity) showing a molecular mass of 27 kDa, with optimal activity at pH 8.0 and and temperature of 37 &#176;C. …”
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Development of a single-chain fragment variable fused-mutant HALT-1 recombinant immunotoxin against G12V mutated KRAS colorectal cancer cells by Michelle Yee Mun Teo, Jeremy Jeack Ceen Ng, Jung Yin Fong, Jung Shan Hwang, Adelene Ai-Lian Song, Renee Lay Hong Lim, Lionel Lian Aun In

“…Anti-mKRAS G12V-34 scFv fragment was fused to mHALT-1 toxin and cloned in pET22b vector with expression as inclusion bodies in E. coli BL21(DE3) (molecular weight of ~46.8 kDa). After successful solubilization and refolding, the mHALT-1-scFv immunotoxin exhibited cytotoxic effects on SW-480 colorectal cancer cells with IC50 of 25.39 μg/mL, with minimal cytotoxicity effect on NHDF cells. …”
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Intestinal segment and vitamin D3 concentration affect gene expression levels of calcium and phosphorus transporters in broiler chickens by Jincheng Han, Lihua Wu, Xianliang Lv, Mengyuan Liu, Yan Zhang, Lei He, Junfang Hao, Li Xi, Hongxia Qu, Chuanxin Shi, Zhiqiang Li, Zhixiang Wang, Fei Tang, Yingying Qiao

“…Results in experiment 1 showed that the mRNA levels of calcium-binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), plasma membrane calcium ATPase 1b (PMCA1b), and IIb sodium-phosphate cotransporter (NaPi-IIb) were the highest in the broiler duodenum. …”
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A New C-Type Lectin Homolog SpCTL6 Exerting Immunoprotective Effect and Regulatory Role in Mud Crab Scylla paramamosain by Wanlei Qiu, Fangyi Chen, Fangyi Chen, Fangyi Chen, Roushi Chen, Shuang Li, Xuewu Zhu, Ming Xiong, Ming Xiong, Ming Xiong, Ke-Jian Wang, Ke-Jian Wang, Ke-Jian Wang

“…SpCTL6 was predicted to have a 17 aa signal peptide and its mature peptide was 144 aa (MW 16.7 kDa) with pI value of 5.22. It had typical CTL structural characteristics, such as a single C-type lectin-like domain, 4 conserved cysteines, similar tertiary structure to that of vertebrate CTLs and a mutated Ca2+ binding motif Gln-Pro-Thr (QPT), clustering into the same branch as the crustacean CTLs. …”
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The Elicitor Protein AsES Induces a Systemic Acquired Resistance Response Accompanied by Systemic Microbursts and Micro–Hypersensitive Responses in Fragaria ananassa by Verónica Hael-Conrad, Silvia Marisa Perato, Marta Eugenia Arias, Martín Gustavo Martínez-Zamora, Pía de los Ángeles Di Peto, Gustavo Gabriel Martos, Atilio Pedro Castagnaro, Juan Carlos Díaz-Ricci, Nadia Regina Chalfoun

“…The elicitor AsES (Acremonium strictum elicitor subtilisin) is a 34-kDa subtilisin-like protein secreted by the opportunistic fungus Acremonium strictum. …”
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