Identification and mechanistic basis of non-ACE2 blocking neutralizing antibodies from COVID-19 patients with deep RNA sequencing and molecular dynamics simulations by Alger M. Fredericks, Kyle W. East, Yuanjun Shi, Jinchan Liu, Federica Maschietto, Alfred Ayala, William G. Cioffi, Maya Cohen, William G. Fairbrother, Craig T. Lefort, Gerard J. Nau, Mitchell M. Levy, Jimin Wang, Victor S. Batista, George P. Lisi, Sean F. Monaghan

“…Here, we identified initially elicited NAbs (Ig Heavy, Ig lambda, Ig kappa) in response to COVID-19 infection in patients admitted to the intensive care unit at a single center with deep RNA sequencing (>100 million reads) of peripheral blood as a diagnostic tool for predicting the severity of the disease and as a means to pinpoint specific compensatory NAb treatments. …”
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CARDIOVACULAR PATHOLOGY AND FRACTURE PREDICTION IN MEN OVER 45 by Ekaterina Yuryevna Orlova, Natalia Anatolyevna Khramtsova

“…The canonical correlation coefficient R was 0.841. Wilkson's lambda statistics values of 0.292 indicate good discrimination (with χ² - 223.7 p<0.01). …”
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Black carbon aerosol in winter northeastern Qinghai–Tibetan Plateau, China: the source, mixing state and optical property by Q. Y. Wang, R.-J. Huang, J. J. Cao, X. X. Tie, H. Y. Ni, Y. Q. Zhou, Y. M. Han, T. F. Hu, C. S. Zhu, T. Feng, N. Li, J. D. Li

“…The rBC mass absorption cross section (MAC_rBC) at λ = 532 nm was slightly larger in clean days (14.9 m² g^−1) than during the pollution episode (9.3 m² g^−1), likely due to the effects of brown carbon and the uncertainty of the MAC_rBC calculation. …”
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LINFOMA PRIMÁRIO DE TESTÍCULO, RELATO DE UM CASO by BP Vasconcelos, AM Peixinho, JVL Amaral, BN Nascimento, MA Gonçalves, MG Cliquet

“…Apresentou os marcadores CD20, BCL2, KI67 80%, PAX5 positivo, lambda monoclonal positivo e kappa monoclonal negativo. …”
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APLASIA PURA DE CÉLULA VERMELHA (APCV) ASSOCIADA A GAMOPATIA MONOCLONAL DE SIGNIFICADO INDETERMINADO (MGUS): UM RELATO DE CASO by TDS Baldanzi, CBS Sola, AGL Mohr, MC Guedes, AVCDS Gasparine, R Galli, LF Soares, LMC Borges

“…Solicitado então eletroforese de proteínas (EP) que foi positiva para proteínas monoclonais na concentração de 25,7% e imunofixação sérica (IFS) demonstrou presença de 2 componentes monoclonais: Igg/LAMBDA. Paciente iniciou, então, tratamento com Bortezomibe 1,3 mg/m3 semanal. …”
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Design and experimental validation of OPERA_MET-A panel for deep methylation analysis by next generation sequencing by Federico Pio Fabrizio, Stefano Castellana, Flavia Centra, Angelo Sparaneo, Mario Mastroianno, Tommaso Mazza, Michelina Coco, Domenico Trombetta, Nicola Cingolani, Antonella Centonza, Paolo Graziano, Evaristo Maiello, Vito Michele Fazio, Vito Michele Fazio, Vito Michele Fazio, Lucia Anna Muscarella

“…The conversion efficiency, determined by spiking-in unmethylated Lambda DNA into each sample before the bisulfite conversion process, was >97% for all samples. …”
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Many-to-Many Disjoint Paths in Augmented Cubes With Exponential Fault Edges by Mingzu Zhang, Wenhuan Ma, Tianlong Ma

“…Motivated by the well-known Menger theorem, we find that the maximum cardinality of edge disjoint paths connecting any two disjoint connected subgraphs with <inline-formula> <tex-math notation="LaTeX">$g$ </tex-math></inline-formula> vertices in <inline-formula> <tex-math notation="LaTeX">$G$ </tex-math></inline-formula> can also define by the minimum modified edge-cut, called the <inline-formula> <tex-math notation="LaTeX">$g$ </tex-math></inline-formula>-extra edge-connectivity of <inline-formula> <tex-math notation="LaTeX">$G~(\lambda _{g}(G))$ </tex-math></inline-formula>. It is the cardinality of the minimum set of edges in <inline-formula> <tex-math notation="LaTeX">$G$ </tex-math></inline-formula>, if such a set exists, whose deletion disconnects <inline-formula> <tex-math notation="LaTeX">$G$ </tex-math></inline-formula> and leaves every remaining component with at least <inline-formula> <tex-math notation="LaTeX">$g$ </tex-math></inline-formula> vertices. …”
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CITOMETRIA DE FLUXO COM MARCAÇÃO EPCAM EM LÍQUIDO CEFALORRAQUIDIANO EM NEOPLASIAS NÃO HEMATOLÓGICAS by LGF Cortes, GBP Fernandes, ACV Lima, EX Souto, AC Vaz, LC Bento, FA Sousa, NM Milan, MS Passaro, NS Bacal

“…Painel utilizado: CD36, ROR1, CD38, CD10, CD56, CD326, CD71, CD81, CD45, CD138, CD8+Lambda, CD56+Kappa, CD4, CD19, CD3+CD14, CD38, CD20, CD45. …”
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AMILOIDOSE NODULAR PULMONAR: UMA ABORDAGEM CUIDADOSA. by LF Soares, TDS Baldanzi, AVCDS Gasparine, AC Ronconi, MC Guedes, LMC Borges, R Galli, BFB Spinelli

“…Pela Hematologia, em exames de investigação revelou-se presença de elevação de imunoglobulina G – 3.457 e de B2microglobulina – 3.593, pico de base ampla na região das gamaglobulinas (3,16 g/dl) em eletroforese de proteínas sérica, presença de banda monoclonal IgG/lambda à imunoeletroforese e ausência de proteinúria. …”
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LEUCEMIA DE CÉLULAS PLASMÁTICAS: RELATO DE CASO by NL Silva, RDS Souza, IO Silva, APS Silva, VVS Lemos, JLJ Santos, GS Cruz, J Brunow, DM Schimieguel

“…Diagnóstico de LCP confirmado em outubro/2021 pela análise morfológica do sangue periférico, mielograma e imunofenotipagem por citometria de fluxo multiparamétrica com presença de 78,1% de plasmócitos monoclonais com fenótipo CD45-/CD38/CD138 secretores de cadeia leve lambda. O tratamento inicial foi baseado no protocolo quimioterápico DV-PACE (4 ciclos) até remissão completa (RC). …”
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IMPORTÂNCIA DA EXPRESSÃO DO CD200 POR CITOMETRIA DE FLUXO NO DIAGNOSTICO DIFERENCIAL DE DOENÇAS LINFOPROLIFERATIVAS CRÔNICAS NO RIO GRANDE DO NORTE by RE Martins, IAF Bahia, FCM Theodoro, GHM Oliveira, MDGP Araújo, RDA Soares, RD Lima, DGB Albuquerque, AFA Dantas, GBC Junior

“…No qual, avaliamos a expressão do CD200 por citometria de fluxo (CF) em linfócitos de sangue periférico em 604 pacientes com DLPC-B com um painel de anticorpos monoclonais (AcMo) que detectam antígenos Pam-T (CD3, CD5, CD7, CD2, TCD4, CD8, CD1a, CD28, TCL1, TCR alfa/beta e TCR gama/delta), Pam- B (CD19, CD20, CD22, CD23, IgM, anti-Kappa, anti-Lambda, CD200, CD79b, FMC7 e CD27, além do CD38, HLADr, CD10, CD81, CD138, CD16-56 e CD45. …”
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A Hybrid Mutual Coupling Reduction Technique in a Dual-Band MIMO Textile Antenna for WBAN and 5G Applications by Hamza A. Mashagba, Hasliza A Rahim, Ismahayati Adam, Mohd Haizal Jamaluddin, Mohd Najib Mohd Yasin, Muzammil Jusoh, Thennarasan Sabapathy, Mohamedfareq Abdulmalek, Azremi Abdullah Al-Hadi, Arif Mawardi Ismail, Ping Jack Soh

“…This hybrid technique results in a simple structure, while enabling significant reduction of mutual coupling (MC) between the closely spaced patches (up to <inline-formula> <tex-math notation="LaTeX">$0.1\lambda$ </tex-math></inline-formula>). This technique combines a line patch and a patch rotation technique, explained as follows. …”
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INFLUÊNCIA DOS ANTICORPOS ANTI-HNA-3 NA REJEIÇÃO AGUDA DO TRANSPLANTE RENAL by JO Martins, HTS Junior, E Moritz, AJ Salum, R Marco, RF Machado, HMS Proença, MG Lima, JOM Pestana, JO Bordin

“…As amostras testadas foram coletadas antes da realização do transplante renal e, a pesquisa de anticorpos anti-HNA-3 foi realizada através das seguintes técnicas: Teste de Aglutinação de Granulócitos (GAT), teste de Imunofluorescência de Granulócitos (Flow-GIFT) e técnica baseada em microesferas (kit LABSCreen Multi, One Lambda). A genotipagem HNA-3 dos receptores e dos doadores do transplante renal foi realizada pela técnica de polimorfismo de fragmentos de restrição utilizando a reação em cadeia da polimerase (PCR–RFLP). …”
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LINFOMA DE ZONA MARGINA EXTRANODAL LOCALIZADO EM HIPÓFISE E LEPTOMENINGE: UM RELATO DE CASO by FGB Chaves, AL Oliveira, ACLD Nascimento, RS Kojima, BFC Galvão, LFBH Junior, AID Ennes, GA Vieira, ACA Silveira, N Hamerschlak

“…O exame de líquor evidenciou 88,4% de linfócitos B maduros com restrição de cadeia leve lambda, compatíveis com infiltração leptomenígea do linfoma. …”
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SARS-CoV-2-specific CD4+ T cells are associated with long-term persistence of neutralizing antibodies by Zhongfang Wang, Xiaoyun Yang, Xinyue Mei, Yumin Zhou, Zhiqiang Tang, Guichang Li, Jiaying Zhong, Mengqiu Yu, Mingzhu Huang, Xiaoling Su, Bijia Lin, Pengxing Cao, Ji Yang, Pixin Ran

“…Interestingly, the level of neutralizing antibodies at 12 months after disease onset was significantly lower than that at 2 months but more broadly neutralized SARS-CoV-2 variants, including Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2), and Lambda (C.37). Significant immune escape by the Omicron variant (B.1.1.529) was also observed 2 months post-recovery. …”
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RELATO DE CASO: SÍNDROME DE BING–NEEL by ET Calvache, TL Secchi, MC Augustin, ADT Calvache, DH Catelli

“…Exames: Hb 11.0 g/dL, leucócitos 3.410/uL, plaquetas 57.000/uL, LDH 355 U/L, eletroforese de proteínas sérica e imunofixação banda monoclonal IgM Kappa de 0.55 g/dL, IgA < 5 mg/dL, IgG < 108 mg/dL, IgM 1.050 mg/dL, relação Kappa/Lambda 1.24. Demais exames sem alterações significativas. …”
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An ultrapotent RBD-targeted biparatopic nanobody neutralizes broad SARS-CoV-2 variants by Xiaojing Chi, Xinhui Zhang, Shengnan Pan, Yanying Yu, Yujin Shi, Tianli Lin, Huarui Duan, Xiuying Liu, Wenfang Chen, Xuehua Yang, Lan Chen, Xiaoqian Dong, Lili Ren, Qiang Ding, Jianwei Wang, Wei Yang

“…Using pseudovirion-based and trans-complementation SARS-CoV-2 tools, we determine that the Nb1–Nb2 broadly neutralizes multiple SARS-CoV-2 variants at sub-nanomolar levels, including Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Delta (B.1.617.2), Lambda (C.37), Kappa (B.1.617.1), and Mu (B.1.621). …”
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Une collection authentiquée d'actes du couvent de Patmos et ses notices dorsales (XIe s.). Notes de diplomatique byzantine by Era L. VRANOUSSI

“…</p><p>Suit l'&eacute;dition des documents ci-dessous: un registre de Stylos, compos&eacute; en 1506, comprenant la description des limites du m&eacute;toque et de sa propri&eacute;t&eacute; terrienne (&sigma;ώ&chi;&omega;&rho;&alpha;, &mu;&omicron;ῖ&rho;&alpha;&iota;); la lettre du doge Michael Steno (1401), qui r&eacute;voque la d&eacute;cision d'accorder le m&eacute;toque de Stylos &agrave; l'&eacute;v&ecirc;que de Calamon (ἐ&pi;ί&sigma;&kappa;&omicron;&pi;&omicron;&sigmaf; &Kappa;&alpha;&lambda;&alpha;&mu;ῶ&nu;&omicron;&sigmaf;) Antonio de Bellan&ccedil;inis; la lettre de l'higoum&egrave;ne Jean du monast&egrave;re de Patmos au doge de Venise Marino Grimani, le f&eacute;licitant pour son &eacute;lection (1595); le document de l'an 1577, conservant les privil&egrave;ges de 1481 et 1486 accord&eacute;s &agrave; Patmos par Venise.…”
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Development and characterization of type I interferon receptor knockout sheep: A model for viral immunology and reproductive signaling by Christopher J. Davies, Christopher J. Davies, Zhiqiang Fan, Kira P. Morgado, Kira P. Morgado, Ying Liu, Misha Regouski, Qinggang Meng, Aaron J. Thomas, Aaron J. Thomas, Sang-Im Yun, Byung-Hak Song, Jordan C. Frank, Iuri V. Perisse, Arnaud Van Wettere, Young-Min Lee, Irina A. Polejaeva

“…Presumably, these sheep compensated for a lack of type I IFN signaling using the type II, IFN-gamma and type III, IFN-lambda pathways. These sheep constitute a unique model for studying the pathogenesis of viral infection. …”
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Development and application of an efficient recombineering system for Burkholderia glumae and Burkholderia plantarii by Ruijuan Li, Hongbo Shi, Xiaoyu Zhao, Xianqi Liu, Qiong Duan, Chaoyi Song, Hanna Chen, Wentao Zheng, Qiyao Shen, Maoqin Wang, Xue Wang, Kai Gong, Jia Yin, Youming Zhang, Aiying Li, Jun Fu

“…Summary The lambda phage Red proteins Redα/Redβ/Redγ and Rac prophage RecE/RecT proteins are powerful tools for precise and efficient genetic manipulation but have been limited to only a few prokaryotes. …”
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