Transcriptomic Analysis of Mineralized Adipose-Derived Stem Cell Tissues for Calcific Valve Disease Modelling by Alyssa Brodeur, Vincent Roy, Lydia Touzel-Deschênes, Stéphanie Bianco, Arnaud Droit, Julie Fradette, Jean Ruel, François Gros-Louis

“…In this study, we describe a comprehensive transcriptomic analysis of tissue-engineered, scaffold-free, ASC-embedded mineralized tissue sheets using bulk RNA sequencing. Bioinformatic and gene set enrichment analyses revealed the up-regulation of genes associated with the organization of the extracellular matrix (ECM), suggesting that the ECM could play a vital role in the enhanced mineralization observed in these tissue-engineered ASC-embedded sheets. …”
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Human Dental Pulp Stem Cells Differentiate into Cementoid-Like-Secreting Cells on Decellularized Teeth Scaffolds by Manuel Mata, Santiago Peydró, José Javier Martín de Llano, María Sancho-Tello, Carmen Carda

“…Periodontitis is a common inflammatory disease that in some cases can cause tooth loss. Cementum is a mineralized tissue that forms part of the insertion periodontium and serves to fix the teeth to the alveolar bone. …”
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Poly(Aspartic Acid) Promotes Odontoblast-like Cell Differentiation in Rat Molars with Exposed Pulp by Fernanda Furuse Ventura dos Santos, Stefan Habelitz, Fábio Dupart Nascimento, Victor Elias Arana-Chavez, Roberto Ruggiero Braga

“…Results: At 7 days, an acute inflammatory infiltrate and the presence of disorganized mineralized tissue were observed in all groups. At 21 days, the quality and thickness of the reparative dentin in treated groups were superior to the control, and bacterial contamination was observed in two MTA-pAsp specimens. …”
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Establishing Criteria for Human Mesenchymal Stem Cell Potency by Samsonraj, Rebekah M., Rai, Bina, Sathiyanathan, Padmapriya, Puan, Kia Joo, Rötzschke, Olaf, Hui, James H., Raghunath, Michael, Stanton, Lawrence Walter, Nurcombe, Victor, Cool, Simon M.

“…However, high-growth capacity MSCs produced approximately double the volume of mineralized tissue compared to low-growth capacity MSCs when assessed for ectopic bone-forming ability. …”
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The role of sclerostin and PGC-1 alpha in the pathogenesis of osteoporosis by Armine V. GRIGORYAN, Tatyana M. BETOVA, Theresa B. DINEVA, Savelina L. POPOVSKA, Radoslav G. TRIFONOV, Aneliya A. DIMITROVA

“…Histomorphological analysis of femur from G1 animals indicated reduced areas of mineralized tissue and bone marrow fatty degeneration. …”
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PLAP-1: A novel molecule regulating homeostasis of periodontal tissues by Satoru Yamada, Masahiro Kitamura, Shinya Murakami

“…PLAP-1 mRNA expression was confirmed in in vitro-maintained PDL cells and was enhanced during the course of the cytodifferentiation of the PDL cells into mineralized tissue-forming cells such as osteoblasts and cementoblasts. …”
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Elongated polyproline motifs facilitate enamel evolution through matrix subunit compaction. by Tianquan Jin, Yoshihiro Ito, Xianghong Luan, Smit Dangaria, Cameron Walker, Michael Allen, Ashok Kulkarni, Carolyn Gibson, Richard Braatz, Xiubei Liao, Thomas G H Diekwisch

“…Together, these findings establish a direct relationship between polyproline tandem repeat fragment assemblies and the evolution and the design of vertebrate mineralized tissue microstructures. Our findings reveal that in the greater context of chordate evolution, the biological control of apatite growth by polyproline-based matrix assemblies provides a molecular basis for the evolution of the vertebrate body plan.…”
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In vitro antibacterial activity of a novel resin-based pulp capping material containing the quaternary ammonium salt MAE-DB and Portland cement. by Yanwei Yang, Li Huang, Yan Dong, Hongchen Zhang, Wei Zhou, Jinghao Ban, Jingjing Wei, Yan Liu, Jing Gao, Jihua Chen

“…Significant biofilm formation was observed on MTA and Dycal.The synthesized material containing HEMA-BisGMA-TEGDMA resin with MAE-DB as the antimicrobial agent and PC to support mineralized tissue formation inhibited S. mutans biofilm formation even after aging in water for 6 months, but had no inhibitory effect on bacteria in solution. …”
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Dentinogenic Specificity in the Preclinical Evaluation of Vital Pulp Treatment Strategies: A Critical Review by Dimitrios Tziafas, Konstantinos Kodonas

“…Data from the present systematic review have shown that only 30.2% of the 152 experimental histological pulp capping studies described the heterogenic nature of the hard tissue bridge formation, including osteotypic and tubular mineralized tissue. Structural characteristics of the new matrix and the associated formative cells were not provided by the remaining 106 studies. …”
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THE FEATURES OF MORPHOLOGICAL CHANGES IN BONE TISSUE AFTER USING THE MATERIAL BASED ON HYDROXIAPATITE AND POLYLACTIDE by O. V. Skochylo, I. R. Mysula

“…In control groups the regeneration with non-mineralized tissue, similar to structure to the organic matrix of the bone was observed. …”
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The role of FGF-2 and BMP-2 in regulation of gene induction, cell proliferation and mineralization by Hughes-Fulford Millie, Li Chai-Fei

“…Photomicroscopy was used to identify newly mineralized tissue and fluorescence was used to quantify mineralization.…”
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Can bone marrow aspirate concentrate change the mineralization pattern of the anterior maxilla treated with xenografts? A preliminary study by André Antonio Pelegrine, Marcelo Lucchesi Teixeira, Marcelo Sperandio, Thiago Sousa Almada, Karl Erik Kahnberg, Paulo José Pasquali, Antonio Carlos Aloise

“…The specimens were fixed and preserved for histomorphometric evaluation, which included the following parameters: Mineralized tissue (MT) and non-MT (NMT). Cone beam computed tomography was performed at 3 time intervals to measure bone thickness: (1) Before grafting, (2) 4 months and (3) 8 months postgrafting, using localized bone gain (mm) as the outcome variable. …”
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Gelatin-Modified Calcium/Strontium Hydrogen Phosphates Stimulate Bone Regeneration in Osteoblast/Osteoclast Co-Culture and in Osteoporotic Rat Femur Defects—In Vitro to In Vivo Tra... by Benjamin Kruppke, Seemun Ray, Volker Alt, Marcus Rohnke, Christine Kern, Marian Kampschulte, Christiane Heinemann, Matthäus Budak, Josephine Adam, Nils Döhner, Lucretia Franz-Forsthoffer, Thaqif El Khassawna, Christian Heiss, Thomas Hanke, Ulrich Thormann

“…The remaining material in the defects and strontium signals, which originate from areas exceeding the defect area, indicate the incorporation of strontium ions into the surrounding mineralized tissue. Thus, the material inherent properties (release of biologically active ions, solubility and degradability, mechanical strength) directly influenced the cellular reaction in vitro and also bone regeneration in vivo. …”
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Use of Platelet-Rich Fibrin Associated with Xenograft in Critical Bone Defects: Histomorphometric Study in Rabbits by Paulo Wilson Maia, Marcelo Lucchesi Teixeira, Luís Guilherme Scavone de Macedo, Antonio Carlos Aloise, Celio Amaral Passos Junior, Juan Manuel Aragoneses, José Luis Calvo-Guirado, André Antonio Pelegrine

“…However, when comparing the groups without membrane coverage, a statistically significant difference could be observed for the vital mineralized tissue (VMT) and nonmineralized tissue (NMT) parameters, with more VMT in the test group and more NMT in the control group. …”
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In vivo fate of bone marrow mesenchymal stem cells implanted into rat pulpotomized molars by Tomoatsu Kaneko, Phyo Pyai Sone, Su Yee Myo Zaw, Yukikio Sueyama, Zar Chi Thein Zaw, Yamato Okada, Hiroki Murano, Bin Gu, Takashi Okiji

“…Our findings indicated that BM-MSCs could differentiate into cells involved in mineralized tissue formation in the functionally relevant region. …”
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VEGF Over-Expression by Engineered BMSC Accelerates Functional Perfusion, Improving Tissue Density and In-Growth in Clinical-Size Osteogenic Grafts by Rene’ D. Largo, Rene’ D. Largo, Maximilian G. Burger, Maximilian G. Burger, Oliver Harschnitz, Oliver Harschnitz, Conny F. Waschkies, Conny F. Waschkies, Andrea Grosso, Andrea Grosso, Celeste Scotti, Alexandre Kaempfen, Sinan Gueven, Gernot Jundt, Arnaud Scherberich, Dirk J. Schaefer, Andrea Banfi, Andrea Banfi, Nunzia Di Maggio

“…Constructs seeded with VEGF-expressing rBMSC showed significantly increased progenitor survivival, depth of tissue ingrowth and amount of mineralized tissue. Contrast-enhanced MRI after 1 week in vivo showed significantly improved tissue perfusion in the inner layer of the grafts compared to controls. …”
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Evaluation of Pulp Repair after Biodentine^TM Full Pulpotomy in a Rat Molar Model of Pulpitis by Sandra Minic, Marion Florimond, Jérémy Sadoine, Anne Valot-Salengro, Catherine Chaussain, Emmanuelle Renard, Tchilalo Boukpessi

“…We showed that, in this model of controlled pulpitis, pulpotomy with Biodentine^TM allowed the synthesis at the injury site of a mineralized bridge formed from mineralized tissue secreted by cells displaying odontoblastic characteristics. …”
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Changes in scaffold porosity during bone tissue engineering in perfusion bioreactors considerably affect cellular mechanical stimulation for mineralization by Feihu Zhao, Damien Lacroix, Keita Ito, Bert van Rietbergen, Sandra Hofmann

“…Therefore, for achieving more mineralized tissue, the conventional manner of loading the perfusion bioreactors (i.e. constant flow rate/velocity) should be changed to a decreasing flow over time in BTE experiments. …”
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Active and Passive Mineralization of Bio-Gide^® Membranes in Rat Calvaria Defects by Karol Ali Apaza Alccayhuaman, Patrick Heimel, Stefan Tangl, Stefan Lettner, Carina Kampleitner, Layla Panahipour, Ulrike Kuchler, Reinhard Gruber

“…The μCT analysis needs to be interpreted carefully, particularly in defects filled with minced membranes, considering that the mineralized tissue may not necessarily be bone but also the result of passive mineralization. …”
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Elastin-derived scaffolding associated or not with bone morphogenetic protein (BMP) or hydroxyapatite (HA) in the repair process of metaphyseal bone defects. by Marcelo de Azevedo E Sousa Munhoz, Karina Torres Pomini, Ana Maria de Guzzi Plepis, Virginia da Conceição Amaro Martins, Eduardo Gomes Machado, Renato de Moraes, Fernando Bento Cunha, Arnaldo Rodrigues Santos Junior, Guinea Brasil Camargo Cardoso, Marco Antonio Hungaro Duarte, Murilo Priori Alcalde, Daniela Vieira Buchaim, Rogerio Leone Buchaim, Marcelo Rodrigues da Cunha

“…Microtomographically, all groups demonstrated partial closure by mineralized tissue except for the E96/37/HA group with hyperdense thin bridge formation interconnecting the edges of the ruptured cortical. …”
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