Spatial control of biological ligands on surfaces applied to T cell activation by Cai, H, Depoil, D, Muller, J, Sheetz, M, Dustin, M, Wind, S

“…The reagent concentration in self-assembly can be adjusted to ensure single-molecule occupancy for a given dot size. The surrounding glass is backfilled by a protein-repellent layer to prevent nonspecific adsorption. …”

Biochemical and structural studies of ASPP proteins reveal differential binding to p53, p63, and p73. by Robinson, R, Lu, X, Jones, E, Siebold, C

“…The crystal lattice revealed an interaction between eight sequential residues in one iASPP molecule and the p53-binding site of a neighboring molecule. …”

Cell interactions in the immune response in vitro. VI. Mediation by T cell surface monomeric IgM. by Feldmann, M, Cone, R, Marchalonis, J

“…This monomeric IgM molecule had specificity for the activating antigen, and like the mediator of cell interaction, bound cytophilically to macrophages, but not to T or B lymphocytes. …”

Graphene nanogaps and nanoelectric devices by Cully, JJ

“…Electronic devices based on single-molecules or single-nanoparticles offer the potential for highly energy-efficient devices with chemically tunable properties for applications in computing, sensing and memory storage. …”

Self-assembly of a strapped linear porphyrin oligomer on HOPG by Bellamy-Carter, A, Roche, C, Anderson, HL, Saywell, A

“…Here we show that highly ordered self-assembled structures can be formed via a simple solution deposition protocol; for a strapped linear porphyrin oligomer adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate. Two distinct molecule–molecule interactions are observed to drive the formation of two molecular phases (‘Interdigitated’ and ‘Bridge-stabilised’) characterised by scanning tunnelling microscopy, providing information on the unit cell dimensions and self-assembled structure. …”

Bifunctional ligands for inhibition of tightbinding by Ivan, T, Enkvist, E, Viira, B, Manoharan, G, Raidaru, G, Pflug, A, Alam, K, Zaccolo, M, Engh, R, Uri, A

“…The acknowledged potential of small-molecule therapeutics targeting disease-related protein-protein interactions (PPIs) has promoted active research in this field. …”

Molecular Occupancy of Nanodot Arrays by Cai, H, Wolfenson, H, Depoil, D, Dustin, M, Sheetz, M, Wind, S

“…As an example, the nanoarray platform was used to probe the geometric requirement of T-cell activation at the single-molecule level.…”

Superoxide signaling required for multicellular development of Dictyostelium by Bloomfield, G, Pears, C

“…We report here the generation of superoxide as a signalling molecule in early development of Dictyostelium discoideum. …”

Primary structure of ICAM-1 demonstrates interaction between members of the immunoglobulin and integrin supergene families. by Staunton, D, Marlin, S, Stratowa, C, Dustin, M, Springer, T

“…Intercellular adhesion molecule 1 (ICAM-1) is a 90 kd inducible surface glycoprotein that promotes adhesion in immunological and inflammatory reactions. …”

Transcription initiation at a consensus bacterial promoter proceeds via a 'bind-unwind-load-and-lock' mechanism by Mazumder, A, Ebright, RH, Kapanidis, A

“…Despite extensive study, the mechanism of promoter unwinding has remained unclear, in part due to the transient nature of intermediates on path to RPo. Here, using single-molecule unwinding-induced fluorescence enhancement to monitor promoter unwinding, and single-molecule fluorescence resonance energy transfer to monitor RNAP clamp conformation, we analyze RPo formation at a consensus bacterial core promoter. …”

Towards a structure of the HIV-1 envelope glycoprotein gp120: an immunochemical approach. by Moore, J, Jameson, B, Sattentau, Q, Willey, R, Sodroski, J

“…First, the regions of gp120 that are accessible on the surface of the native molecule, and those that are internal but exposed after denaturation, are identified. …”

Fluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasers. by Fölling, J, Belov, V, Riedel, D, Schönle, A, Egner, A, Eggeling, C, Bossi, M, Hell, S

“…This bright marker enables strict control of on/off switching and provides single-molecule localization precision down to 15 nm in the focal plane. …”

Molecular mechanisms of cytoadherence in malaria. by Ho, M, White, N

“…Microbial pathogens subvert host adhesion molecules to disseminate or to enter host cells to promote their own survival. …”

A neutron reflectivity study of the adsorption of aerosol-OT on self-assembled monolayers on silicon by Fragneto, G, Li, Z, Thomas, R, Rennie, A, Penfold, J

“…At its critical micelle concentration (cmc) AOT forms a monolayer with an area per molecule of 80 ± 5 Å2 and a thickness of 15 ± 2 Å. …”

NMR crystallography of oxybuprocaine hydrochloride, Modification II degrees. by Harris, R, Cadars, S, Emsley, L, Yates, JR, Pickard, C, Jetti, R, Griesser, U

“…It shows crystallographic splittings arising from the fact that there are two molecules, with substantially different conformations, in the asymmetric unit. …”

Making a little affinity go a long way: a topological view of LFA-1 regulation. by Dustin, M

“…Interactions of adhesion molecules are governed by the law of mass action and the less understood rules of apposed biological membranes. …”

Structural characterization of the GSK-3beta active site using selective and non-selective ATP-mimetic inhibitors. by Bertrand, J, Thieffine, S, Vulpetti, A, Cristiani, C, Valsasina, B, Knapp, S, Kalisz, H, Flocco, M

“…In addition, a conserved water molecule near Thr138 is identified that can serve a functional role in inhibitor binding. …”

Simultaneous quantification of the interplay between molecular turnover and cell mechanics by AFM-FRAP by Skamrahl, M, Colin-York, H, Barbieri, L, Fritzsche, M

“…Simultaneous quantification of the relationship between molecule kinetics and cell mechanics may thus open-up unprecedented insights into adaptive mechanobiological mechanisms of cells.…”

Molecular mechanisms of cytoadherence in malaria by May, H, White, N

“…Microbial pathosens subvert host adhesion molecules to dissemi-nate or to enter host cells to promote their own survival. …”

Novel, cross-restricted, conserved, and immunodominant cytotoxic T lymphocyte epitopes in slow progressors in HIV type 1 infection. by Goulder, P, Bunce, M, Krausa, P, McIntyre, K, Crowley, S, Morgan, B, Edwards, A, Giangrande, P, Phillips, R, McMichael, A

“…Long-term nonprogressors (LTNPs) or slow progressors (SPs) in HIV infection may make qualitatively different CTL responses compared to those generated by seropositive individuals who progress to disease at a faster rate. The class I molecule HLA-B*57 has been identified as one restriction element overrepresented in SP groups studied, and, together with the closely related molecule HLA-B*58, occurs commonly in ethnic groups where HIV is most prevalent. …”