Effects of ionic strength on gating and permeation of TREK-2 K2P channels by Conrad, LJ, Proks, P, Tucker, SJ

“…Recently, we found that this action can be modulated by small molecule agonists (e.g. BL1249) which appear to have an electrostatic influence on K+ binding within the inner cavity and produce an increase in the single-channel conductance of TREK-2 channels. …”

Stereodynamics in NO(X) + Ar inelastic collisions. by Brouard, M, Chadwick, H, Gordon, S, Hornung, B, Nichols, B, Aoiz, F, Stolte, S

“…However, classical effects cannot account quantitatively for the differential steric asymmetry observed in NO(X) + Ar collisions, which reflects quantum interference from scattering at either end of the molecule. This quantum interference effect is dominated by the repulsive region of the potential.…”

The effect of tumour necrosis factor-alpha (TNF-alpha) muteins on human neutrophils in vitro. by Tchorzewski, H, Zeman, K, Kantorski, J, Paleolog, E, Kahan, M, Feldmann, M, Kwinkowski, M, Guga, P, Szymanska, B, Parniewski, P, Wilk, A, Jarosz, J

“…To verify the suggestion that the receptor binding domain includes the region close to the N-terminus of the TNF-alpha molecule, four TNF-alpha derivatives termed muteins were constructed, using a synthetic cDNA fragment substituting the N-terminal 3-7 selected hydrophilic or hydrophobic amino acids in the original TNF-alpha genomic DNA. …”

Statistical correlation and projection methods for improved information recovery from diffusion-edited NMR spectra of biological samples. by Smith, L, Maher, A, Cloarec, O, Rantalainen, M, Tang, H, Elliott, P, Stamler, J, Lindon, J, Holmes, E, Nicholson, J

“…This method relies on calculation and display of the covariance of signal intensities from the various nuclei on the same molecule across a series of spectra collected under different pulsed field gradient conditions that differentially attenuate the signal intensities according to translational molecular diffusion rates. …”

DmWRNexo is a 3'-5' exonuclease: phenotypic and biochemical characterization of mutants of the Drosophila orthologue of human WRN exonuclease. by Boubriak, I, Mason, P, Clancy, D, Dockray, J, Saunders, R, Cox, L

“…Molecular modelling suggests that D229 lies towards the outside of the molecule distant from the nuclease active site. We have produced recombinant protein of the D229V mutant, assayed its nuclease activity in vitro, and compared activity with that of wild type DmWRNexo and a D162A E164A double active site mutant we have created. …”

Neutron reflection study of phenol adsorbed at the surface of its aqueous solutions: An unusual adsorbed layer by Li, Z, Thomas, R, Rennie, A, Penfold, J

“…It is suggested that in such a diffuse layer the phenol molecules are unlikely to be inclined at a single angle to the surface normal, as suggested from the interpretation of second harmonic generation measurements. …”

Product state and speed distributions in photochemical triple fragmentations. by de Wit, G, Heazlewood, B, Quinn, MS, Maccarone, A, Nauta, K, Reid, SA, Jordan, M, Kable, S

“…The PST model starts with a calculation of two-body fragmentation (2F) of a generic molecule, ABC into AB + C. Every AB fragment with sufficient energy to undergo subsequence spontaneous dissociation is allowed to dissociate and the PST distribution of energy into A + B products is calculated for every initial AB state. …”

An electrochemical thermometer: voltammetric measurement of temperature and its application to amperometric gas sensing. by Xiong, L, Fletcher, A, Ernst, S, Davies, S, Compton, R

“…In the first part of this work, we introduce temperature sensing systems based upon voltammetric responses of both single molecule (1,2-diferrocenylethylene in 1-propyl-3-methylimidazolium bistrifluoromethylsulfonylimide) and two independent molecules (decamethylferrocene and N,N,N',N'-tetramethyl-p-phenylenediamine in 1-ethyl-3-methylimidazolium tetracyanoborate) respectively. …”

Molecular photobleaching kinetics of Rhodamine 6G by one- and two-photon induced confocal fluorescence microscopy. by Eggeling, C, Volkmer, A, Seidel, C

“…Under high-excitation irradiance conditions in one- and two-photon induced fluorescence microscopy, the photostability of fluorescent dyes is of crucial importance for the detection sensitivity of single molecules and for the contrast in fluorescence imaging. …”

Preventing acute gut wall damage in infectious diarrhoeas with glycosylated dendrimers. by Teo, I, Toms, S, Marteyn, B, Barata, T, Simpson, P, Johnston, K, Schnupf, P, Puhar, A, Bell, T, Tang, C, Zloh, M, Matthews, S, Rendle, P, Sansonetti, P, Shaunak, S

“…Computational modelling studies identified a 3.3 kDa polypropyletherimine (PETIM)-DG as the smallest likely bioactive molecule. In human monocytes, high purity PETIM-DG potently inhibited Shigella Lipid A-induced IL-6 expression. …”

Pax6 is required for the normal development of the forebrain axonal connections

“…The aberrant development of axonal connections in absence of Pax6 function appear to be related to ultrastructural defects of cells along the PSPB, as well as to a failure of axonal guidance molecule expression, including Sema3c and Sema5a. …”

Induction and abrogation of LACK reactive cells in the evolution of human leishmaniasis. by Maasho, K, Wolday, D, Edjigu, M, Söderström, K, Britton, S, Akuffo, H

“…Since cure from leishmaniasis often results in life-long protection, and active but not cured patients showed in vitro responses to LACK stimulation, questions arose as to how this highly immunodominant molecule functions during human leishmanisasis. Some possible mechanisms are discussed.…”

Experimentally determining the exchange parameters of quasi-two-dimensional Heisenberg magnets by Goddard, P, Singleton, J, Sengupta, P, McDonald, R, Lancaster, T, Blundell, S, Pratt, F, Cox, S, Harrison, N, Manson, J, Southerland, H, Schlueter, J

“…Our results suggest the superexchange efficiency of the [Cu(HF2) (pyz) 2]X family of compounds (where X can be ClO4, BF 4, PF6, SbF6 and AsF6) might be controlled by the tilting of the pyz molecule with respect to the 2D planes. © IOP Publishing Ltd and Deutsche Physikalische Gesellschaft.…”

TNFR2 ligation in human T regulatory cells enhances IL2-induced cell proliferation through the non-canonical NF-κB pathway by Wang, J, Ferreira, R, Lu, W, Farrow, S, Downes, K, Jermutus, L, Minter, R, Al-Lamki, RS, Pober, JS, Bradley, JR

“…Ligation of TNFR2 with TNF, which can recognise both TNFR1 and TNFR2, or with a TNFR2-selective binding molecule, DARPin 18 (D18) activates canonical NF-κB signalling, assessed by IκBα degradation, and the magnitude of the response correlates with the level of TNFR2 expression. …”

Detection of ubiquitin-proteasome enzymatic activities in cells: application of activity-based probes to inhibitor development. by Kramer, H, Nicholson, B, Kessler, B, Altun, M

“…This method enables cellular validation of the specificity of small molecule DUB inhibitors. GENERAL SIGNIFICANCE: Molecular probes combined with mass spectrometry-based proteomics and cellular assays represent a powerful approach for discovery and functional validation, a concept that can be expanded to other enzyme classes. …”

Stereospecific targeting of MTH1 by (S)-crizotinib as an anticancer strategy. by Huber, K, Salah, E, Radic, B, Gridling, M, Elkins, J, Stukalov, A, Jemth, A, Göktürk, C, Sanjiv, K, Strömberg, K, Pham, T, Berglund, U, Colinge, J, Bennett, K, Loizou, J, Helleday, T, Knapp, S, Superti-Furga, G

“…Cellular models of RAS-dependent cancers have been used to identify experimental small molecules, such as SCH51344, but their molecular mechanism of action remains generally unknown. …”

High level transcription of the complement regulatory protein CD59 requires an enhancer located in intron 1. by Tone, M, Diamond, L, Walsh, L, Tone, Y, Thompson, SA, Shanahan, E, Logan, J, Waldmann, H

“…CD59 is a complement regulatory protein and may also act as a signal-transducing molecule. CD59 transgenic mice have been generated using a CD59 minigene (CD59 minigene-1). …”

Human invariant NKT cells display alloreactivity instructed by invariant TCR-CD1d interaction and killer Ig receptors. by Patterson, S, Chaidos, A, Neville, D, Poggi, A, Butters, T, Roberts, I, Karadimitris, A

“…Central to their function is the interaction of the invariant TCR with glycosphingolipid (GSL) ligands presented by the nonpolymorphic MHC class I molecule CD1d and their ability to secrete rapidly large amounts of immunomodulatory cytokines when activated. …”

The adsorption behavior of ionic surfactants and their mixtures with nonionic polymers and with polyelectrolytes of opposite charge at the air-water interface. by Bahramian, A, Thomas, R, Penfold, J

“…The surface phase approach of Butler has been used to derive a model of the surface tension (ST) of surfactant solutions in terms of the ST of the surfactant in the absence of water, an area parameter corresponding approximately to the limiting area per molecule, and the critical micelle concentration (CMC). …”

Haplotypic diversity in human CEACAM genes: effects on susceptibility to meningococcal disease by Callaghan, M, Rockett, K, Banner, C, Haralambous, E, Betts, H, Faust, S, Maiden, M, Kroll, J, Levin, M, Kwiatkowski, D, Pollard, A

“…Adhesion between the opacity-associated adhesin (Opa) proteins of Neisseria meningitidis and human carcino-embryonic antigen cell adhesion molecule (CEACAM) proteins is an important stage in the pathogenesis of meningococcal disease, a globally important bacterial infection. …”