Targeted erythropoietin selectively stimulates red blood cell expansion in vivo by Burrill, Devin R., Vernet, Andyna, Collins, James J., Silver, Pamela A., Way, Jeffrey C.

“…Here we applied this approach in vivo to the anemia drug erythropoietin (EPO), to direct its activity to EPO receptors (EPO-Rs) on red blood cell (RBC) precursors and prevent interaction with EPO-Rs on nonerythroid cells, such as platelets. Our engineered EPO molecule was mutated to weaken its affinity for EPO-R, but its avidity for RBC precursors was rescued via tethering to an antibody fragment that specifically binds the human RBC marker glycophorin A (huGYPA). …”
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Estimation of local concentration from measurements of stochastic adsorption dynamics using carbon nanotube-based sensors by Jang, Hong, Lee, Jay H., Braatz, Richard D

“…This paper proposes a maximum likelihood estimation (MLE) method for estimating time varying local concentration of the target molecule proximate to the sensor from the time profile of monomolecular adsorption and desorption on the surface of the sensor at nanoscale. …”
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A role for the bacterial GATC methylome in antibiotic stress survival by Ross, Christian A, Belenky, Peter, Li, Hu, Cohen, Nadia, Jain, Saloni R., Gutierrez, Arnaud, Collins, James J., Shapiro, Rebecca

“…We explore the role of the bacterial epigenome in antibiotic stress survival using classical genetic tools and single-molecule real-time sequencing to characterize genomic methylation kinetics. …”
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Disruption of Sphingolipid Biosynthesis Blocks Phagocytosis of Candida albicans by Tafesse, Fikadu G., Rashidfarrokhi, Ali, Freinkman, Elizaveta, Dougan, Stephanie, Dougan, Michael, Esteban, Alexandre, Maruyama, Takeshi, Strijbis, Karin, Schmidt, Florian Ingo, Ploegh, Hidde

“…The role of signaling lipid molecules such as phosphoinositides is well established, but the role of membrane sphingolipids in phagocytosis is largely unknown. …”
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Super-resolution imaging of fluorescently labeled, endogenous RNA Polymerase II in living cells with CRISPR/Cas9-mediated gene editing by Cho, Won-ki, Jayanth, Namrata, Mullen, Susan, Tan, Tzer Han, Jung, Yoon, Cisse, Ibrahim I

“…Here we label the endogenous Pol II in mouse embryonic fibroblast (MEF) cells using the CRISPR/Cas9 gene editing system. Using single-molecule based super-resolution imaging in the living cells, we captured endogenous Pol II clusters. …”
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A distant trophoblast-specific enhancer controls HLA-G expression at the maternal–fetal interface by Ferreira, Leonardo M. R., Meissner, Torsten B., Mikkelsen, Tarjei S., Mallard, William, O’Donnell, Charles W., Tilburgs, Tamara, Gomes, Hannah A. B., Camahort, Raymond, Sherwood, Richard I., Rinn, John L., Cowan, Chad A., Strominger, Jack L., Gifford, David K

“…HLA-G, a nonclassical HLA molecule uniquely expressed in the placenta, is a central component of fetus-induced immune tolerance during pregnancy. …”
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Highly Dynamic Interactions Maintain Kinetic Stability of the ClpXP Protease During the ATP-Fueled Mechanical Cycle by Sello, Jason K., Sauer, Robert T., Amor, Alvaro Jorge, Baker, Tania, Schmitz, Karl R.

“…ATP binding to high-affinity subunits of the ClpX hexamer prevents rapid dissociation, but additional subunits must be occupied to promote assembly. Small-molecule acyldepsipeptides, which compete with the IGF loops of ClpX for ClpP-cleft binding, cause exceptionally rapid dissociation of otherwise stable ClpXP complexes, suggesting that the IGF-loop interactions with ClpP must be highly dynamic. …”
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Amino Acid Copolymers That Alleviate Experimental Autoimmune Encephalomyelitis In Vivo Interact with Heparan Sulfates and Glycoprotein 96 in APCs by Koenig, Paul-Albert, Spooner, Eric, Kawamoto, Norio, Strominger, Jack L., Ploegh, Hidde

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Stimuli-responsive self-assembling materials comprising amphiphilic copolymers for localized remotely triggered therapeutic delivery by Collins, Samantha Caitlin

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Clonal Expansion of Lgr5-Positive Cells from Mammalian Cochlea and High-Purity Generation of Sensory Hair Cells by Lenz, Danielle R., McLean, Dalton, Karp, Jeffrey M., Edge, Albert S.B., McLean, Will J., Yin, Xiaolei, Lu, Lin, Langer, Robert S

“…Here, using a small-molecule approach, we show significant expansion (>2,000-fold) of cochlear supporting cells expressing and maintaining Lgr5, an epithelial stem cell marker, in response to stimulation of Wnt signaling by a GSK3β inhibitor and transcriptional activation by a histone deacetylase inhibitor. …”
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IRE1α Induces Thioredoxin-Interacting Protein to Activate the NLRP3 Inflammasome and Promote Programmed Cell Death under Irremediable ER Stress by Lerner, Alana G., Upton, John-Paul, Praveen, P.V.K., Ghosh, Rajarshi, Nakagawa, Yoshimi, Igbaria, Aeid, Shen, Sarah, Nguyen, Vinh, Backes, Bradley J., Heintz, Nathaniel, Greengard, Paul, Hui, Simon, Tang, Qizhi, Trusina, Ala, Oakes, Scott A., Papa, Feroz R., Heiman, Myriam

“…Txnip gene deletion reduces pancreatic β cell death during ER stress and suppresses diabetes caused by proinsulin misfolding in the Akita mouse. Finally, small molecule IRE1α RNase inhibitors suppress TXNIP production to block IL-1β secretion. …”
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Kinetically selective inhibitors of histone deacetylase 2 (HDAC2) as cognition enhancers by Wagner, F. F., Zhang, Y.-L., Fass, D. M., Gale, J. P., Weïwer, M., McCarren, P., Fisher, S. L., Kaya, T., Zhao, W.-N., Reis, S. A., Hennig, K. M., Thomas, M., Lemercier, B. C., Lewis, M. C., Moyer, M. P., Scolnick, E., Haggarty, S. J., Holson, E. B., Joseph, Nadine, Guan, Jisong, Tsai, Li-Huei

“…Aiming towards the development of novel nootropic therapeutics to address the cognitive impairment common to a range of brain disorders, we set out to develop highly selective small molecule inhibitors of HDAC2, a chromatin modifying histone deacetylase implicated in memory formation and synaptic plasticity. …”
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Characterizing the Altered Cellular Proteome Induced by the Stress-Independent Activation of Heat Shock Factor 1 by Ryno, Lisa M., Genereux, Joseph C., Naito, Tadasuke, Morimoto, Richard I., Powers, Evan T., Shoulders, Matthew D., Wiseman, R. Luke

“…Here, we employ a highly controllable, ligand-regulated HSF1 that activates HSF1 to levels compatible with those that could be achieved using selective small molecule HSF1 activators. Using a combination of RNAseq and quantitative proteomics, we define the impact of stress-independent HSF1 activation on the composition of the cellular proteome. …”
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A Caenorhabditis elegans protein with a PRDM9-like SET domain localizes to chromatin-associated foci and promotes spermatocyte gene expression, sperm production and fertility by Engert, Christoph, Droste, Rita, van Oudenaarden, Alexander, Horvitz, Howard Robert

“…To better understand the tissue-specific regulation of chromatin state in cell-fate determination and animal development, we defined the tissue-specific expression of all 36 C. elegans presumptive lysine methyltransferase (KMT) genes using single-molecule fluorescence in situ hybridization (smFISH). …”
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Rapid Optimization of Mcl-1 Inhibitors using Stapled Peptide Libraries Including Non-Natural Side Chains by Letai, Anthony, Rezaei Araghi, Raheleh, Ryan, Jeremy Adam, Keating, Amy E.

“…Library-identified peptide M3d is the most potent molecule yet tested for selectively triggering mitochondrial permeabilization in Mcl-1 dependent cell lines. …”
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A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors by Park, Ryan J, Koundakjian, Dylan, Hultquist, Judd F, Lamothe-Molina, Pedro, Monel, Blandine, Schumann, Kathrin, Yu, Haiyan, Krupzcak, Kevin M, Garcia-Beltran, Wilfredo, Piechocka-Trocha, Alicja, Krogan, Nevan J, Marson, Alexander, Hacohen, Nir, Walker, Bruce D, Wang, Tim, Sabatini, David, Lander, Eric Steven

“…Activated leukocyte cell adhesion molecule (ALCAM) mediates cell aggregation, which is required for cell-to-cell HIV transmission. …”
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Pathobiology of Human Uterine Leiomyosarcoma for Development of Novel Diagnosis and Clinical Therapy by Hayashi, Takuma, Ichimura, Tomoyuki, Kasai, Mari, Gur, Gal, Yaish, Pnina, Zharhary, Dorit, Kanai, Yae, Yaegashi, Nobuo, Konishi, Ikuo, Tonegawa, Susumu

“…PSMB9/β1i is a potential diagnostic-biomarker for human U-LMS, and may be targeted-molecule for a new therapeutic approach. Keywords: PSMB9/β1i; Diagnosis; Mesenchymal tumour; Leiomyosarcoma; Leiomyoma…”
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Highly Stereoselective Heterogeneous Diene Polymerization by Co-MFU-4 by Wu, Zhenwei, Zhang, Guanghui, Miller, Jeffrey T., Dubey, Romain, Comito, Robert J, Rieth, Adam Joseph, Hendon, Christopher H, Dinca, Mircea

“…This work underscores the capacity for small molecule-like tunability and mechanistic tractability available to transition metal catalysis in metal–organic frameworks.…”
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Machine Learning To Predict Cell-Penetrating Peptides for Antisense Delivery by Yao, Monica, Hanson, Gunnar J., Wolfe, Justin, Fadzen, Colin M., Choo, Zi-Ning, Holden, Rebecca Lynn, Pentelute, Bradley L.

“…Cell-penetrating peptides (CPPs) can facilitate the intracellular delivery of large therapeutically relevant molecules, including proteins and oligonucleotides. …”
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Combining NGN2 Programming with Developmental Patterning Generates Human Excitatory Neurons with NMDAR-Mediated Synaptic Transmission by Nehme, Ralda, Zuccaro, Emanuela, Ghosh, Sulagna Dia, Li, Chenchen, Sherwood, John L., Pietilainen, Olli, Barrett, Lindy E., Limone, Francesco, Worringer, Kathleen A., Kommineni, Sravya, Zang, Ying, Cacchiarelli, Davide, Meissner, Alex, Adolfsson, Rolf, Haggarty, Stephen, Madison, Jon, Muller, Matthias, Arlotta, Paola, Fu, Zhanyan, Feng, Guoping, Eggan, Kevin

“…Nehme et al. combine two strong neuralizing factors (transcription factor programming and small molecule patterning) to generate human excitatory neurons from stem cells. …”
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