Lipoprotein complex of equine lysozyme with oleic acid (ELOA) interactions with the plasma membrane of live cells. by Vukojević, V, Bowen, A, Wilhelm, K, Ming, Y, Ce, Z, Schleucher, J, Hore, P, Terenius, L, Morozova-Roche, L

“…We have used methods with single-molecule sensitivity, fluorescence correlation spectroscopy (FCS), fluorescence cross-correlation spectroscopy (FCCS), and confocal laser scanning microscopy (CLSM) imaging by avalanche photodiodes (APD), so-called APD imaging, to study ELOA interactions with the plasma membrane in live PC12 cells. …”

Inhibition of tryptophan-dioxygenase activity increases the antitumor efficacy of immune checkpoint inhibitors by Schramme, F, Crosignani, S, Frederix, K, Hoffmann, D, Pilotte, L, Stroobant, V, Preillon, J, Driessens, G, Van Den Eynde, B

“…Here we described a new small molecule inhibitor of TDO that can modulate kynurenine and tryptophan in plasma, liver and tumor tissue upon oral administration. …”

The HLA-DRB1*15:01-restricted Goodpasture's T cell epitope induces GN. by Ooi, J, Chang, J, O'Sullivan, K, Pedchenko, V, Hudson, BG, Vandenbark, A, Fugger, L, Holdsworth, SR, Kitching, A

“…The target autoantigen in this disease is the noncollagenous domain of the α3 chain of type IV collagen, α3(IV)NC1, but critical early T cell epitopes presented by this human MHC class II molecule are unknown. Here, by immunizing HLA-DRB1*15:01 transgenic mice with whole recombinant α3(IV)NC1 and with overlapping α3(IV)NC1 peptides, we defined a HLA-DRB1*15:01-restricted α3(IV)NC1 T cell epitope (α3136-146) with four critical residues. …”

A study of the structure of biological macromolecules by Bradshaw, J, Bradshaw, Jeremy Peter

“…<p xmlns:etd="http://www.ouls.ox.ac.uk/ora/modsextensions">This thesis describes a study of the structure of the Type I collagen molecule and fibril as they occur in a variety of tissues and, in particular, the role that this protein plays in the molecular basis of connective tissue disease.…”

Inhibition of O-GlcNAc transferase activates tumor-suppressor gene expression in tamoxifen-resistant breast cancer cells by Barkovskaya, A, Seip, K, Prasmickaite, L, Mills, IG, Moestue, SA, Itkonen, HM

“…We show that in vitro development of tamoxifen-resistance is associated with increased sensitivity to the OGT small molecule inhibitor OSMI-1. Global transcriptome profiling revealed that TamS cells adapt to OSMI-1 treatment by increasing the expression of histone genes. …”

Refined crystal structures of Escherichia coli and chicken liver dihydrofolate reductase containing bound trimethoprim. by Matthews, D, Bolin, J, Burridge, J, Filman, D, Volz, K, Kaufman, B, Beddell, C, Champness, J, Stammers, D, Kraut, J

“…Finally, when TMP binds to the chicken holoenzyme, the Tyr-31 side chain undergoes a large conformational change (average movement is 5.4 A for all atoms beyond C beta), rotating down into a new position where it hydrogen bonds via an intervening water molecule to the backbone carbonyl oxygen of Trp-24.…”

Tetrahydrobiopterin supplementation reduces atherosclerosis and vascular inflammation in apolipoprotein E-knockout mice. by Schmidt, T, McNeill, E, Douglas, G, Crabtree, M, Hale, AB, Khoo, J, O'Neill, C, Cheng, A, Channon, K, Alp, N

“…BH4 supplementation significantly reduced VCAM-1 (vascular cell adhesion molecule 1) mRNA levels in aortic endothelial cells, markedly reduced the infiltration of T-cells, macrophages and monocytes into plaques, and reduced T-cell infiltration in the adjacent adventitia, but importantly had no effect on circulating leucocytes. …”

Plasma membrane calcium pump (PMCA4)-neuronal nitric-oxide synthase complex regulates cardiac contractility through modulation of a compartmentalized cyclic nucleotide microdomain. by Mohamed, T, Oceandy, D, Zi, M, Prehar, S, Alatwi, N, Wang, Y, Shaheen, M, Abou-Leisa, R, Schelcher, C, Hegab, Z, Baudoin, F, Emerson, M, Mamas, M, Di Benedetto, G, Zaccolo, M, Lei, M, Cartwright, E, Neyses, L

“…In the heart, in addition to subsarcolemmal calcium transport, PMCA4 acts as a structural molecule that maintains the spatial and functional integrity of the nNOS signaling complex in a defined microdomain. …”

Pathogenic immune responses in Spondyloarthritis by Al-Mossawi, M

“…</p> <p>My data shows that RORγt, the master transcription factor of Th17 cells, can be therapeutically targeted using small molecule inhibitors in-vitro. These inhibitors lead to a specific suppression of both polyfunctional and IL-17A single positive Th17 cells. …”

Effect of oxygen, moisture and illumination on the stability and reliability of dinaphtho[2,3-b:2',3'-f]thieno[3,2-b]thiophene (DNTT) OTFTs during operation and storage. by Ding, Z, Abbas, G, Assender, H, Morrison, J, Yeates, S, Patchett, E, Taylor, D

“…The OTFTs are based on the semiconducting small molecule dinaphtho[2,3-b:2',3'-f]thieno[3,2-b]thiophene (DNTT) deposited onto the surface of a polystyrene-buffered in situ polymerized diacrylate gate insulator. …”

Modulating the immune system by amino acid depletion - IDO and beyond by Vallius, L

“…</p><p> In this study, we investigated the IDO inhibitory properties and mechanism of action of the tryptophan metabolite 3-hydroxyanthranilic acid (3-HAA) that potentially forms a negative feedback loop in the kynurenine pathway. We studied the molecule in enzymatic assays, in live cells and discovered that it inhibits IDO in an indirect way via the formation of hydrogen peroxide. …”

Crystal structure of Escherichia coli ketopantoate reductase at 1.7 A resolution and insight into the enzyme mechanism. by Matak-Vinković, D, Vinković, M, Saldanha, SA, Ashurst, J, von Delft, F, Inoue, T, Miguel, R, Smith, A, Blundell, T, Abell, C

“…They belonged to the tetragonal space group P4(2)2(1)2, with cell parameters: a = b = 103.7 A and c = 55.7 A, accommodating one enzyme molecule per asymmetric unit. The structure of KPR was determined by the multiwavelength anomalous dispersion method using the SeMet protein, for which data were collected to 2.3 A resolution. …”

Remnant epitopes, autoimmunity and glycosylation. by Opdenakker, G, Dillen, C, Fiten, P, Martens, E, Van Aelst, I, Van den Steen, P, Nelissen, I, Starckx, S, Descamps, F, Hu, J, Piccard, H, Van Damme, J, Wormald, M, Rudd, P, Dwek, R

“…The stability, interactions with inhibitors or receptors, and activities of these molecules are fine-controlled by glycosylation. We studied gelatinase B or matrix metalloproteinase-9 (MMP-9) as a glycosylated enzyme involved in autoimmunity. …”

In vivo fluorescence imaging of E-selectin: quantitative detection of endothelial activation in a mouse model of arthritis. by Gompels, L, Madden, L, Lim, N, Inglis, J, McConnell, E, Vincent, T, Haskard, DO, Paleolog, E

“…E-selectin, a leukocyte adhesion molecule expressed on endothelium, is induced by tumor necrosis factor α (TNFα) and other cytokines involved in the pathogenesis of rheumatoid arthritis (RA). …”

Membrane ultrastructure and T cell activation by Pettmann, J, Santos, A, Dushek, O, Davis, S

“…This includes single-molecule localization microscopy, which has been applied to the question of whether TCRs are pre-clustered on resting T cells, and lattice light-sheet microscopy that has enabled imaging of whole cells interacting with antigen-presenting cells. …”

Low-coordinate rare-earth complexes of the asymmetric 2,4-di-tert-butylphenolate ligand prepared by redox transmetallation/protolysis reactions, and their reactivity towards ring-o... by Clark, L, Deacon, G, Forsyth, C, Junk, P, Mountford, P, Townley, J

“…These dimeric complexes contain two bridging and four terminal phenolates, as well as a single coordinated molecule of thf at each metal. A similar structural motif was observed for the products when the reaction was performed in diethyl ether, and in the absence of a solvent, yielding [Nd(2)(dbp)(6)(Et(2)O)(2)] (9) and [Nd(2)(dbp)(6)(dbpH)(2)] (10) respectively. …”

Tumor hypoxia induces nuclear paraspeckle formation through HIF-2α dependent transcriptional activation of NEAT1 leading to cancer cell survival. by Choudhry, H, Albukhari, A, Morotti, M, Hider, S, Moralli, D, Smythies, J, Schödel, J, Green, C, Camps, C, Buffa, F, Ratcliffe, P, Ragoussis, J, Harris, A, Mole, DR

“…We show that the nuclear retention of one such transcript, F11R (also known as junctional adhesion molecule 1, JAM1), in hypoxia is dependent upon the hypoxic increase in NEAT1, thereby conferring a novel mechanism of HIF-dependent gene regulation. …”

Donor brain death aggravates chronic rejection after lung transplantation in rats. by Zweers, N, Petersen, A, van der Hoeven, J, de Haan, A, Ploeg, R, de Leij, L, Prop, J

“…After 6 hr, donor lungs were assessed for influx of leukocytes, expression of cell adhesion molecules, and cytokine mRNA expression. For assessment of the lung after transplantation, lungs from brain-dead F344 rats were transplanted into WKY rats. …”

Proteomic analysis of the cell-surface membrane in chronic lymphocytic leukemia: identification of two novel proteins, BCNP1 and MIG2B. by Boyd, R, Adam, P, Patel, S, Loader, J, Berry, J, Redpath, N, Poyser, H, Fletcher, G, Burgess, N, Stamps, A, Hudson, L, Smith, P, Griffiths, M, Willis, T, Karran, E, Oscier, D, Catovsky, D, Terrett, J, Dyer, M

“…B-cell-specific plasma-membrane proteins are potential targets for either small molecule or antibody-based therapies. We have sought to annotate proteins expressed at the cell surface membrane in patients with chronic lymphocytic leukemia (CLL) using plasma-membrane-based proteomic analysis to identify previously uncharacterized and potentially B-cell-specific proteins. …”

Direct observation and evolution of electronic coupling between organic semiconductors by Kesava, SV, Riede, MK

“…The electronic wave functions of an atom or molecule are affected by its interactions with its environment. …”