Recombinant mycobacterial DNA-binding protein 1 with post-translational modifications boosts IFN-gamma production from BCG-vaccinated individuals’ blood cells in combination with C... by Yuriko Ozeki, Akira Yokoyama, Akihito Nishiyama, Yutaka Yoshida, Yukiko Ohara, Tsukasa Mashima, Chikako Tomiyama, Amina K. Shaban, Atsuki Takeishi, Mayuko Osada-Oka, Takehiro Yamaguchi, Yoshitaka Tateishi, Jun-ichi Maeyama, Mariko Hakamata, Hiroshi Moro, Toshiaki Kikuchi, Daisuke Hayashi, Fumiko Suzuki, Toshiko Yamamoto, Sumiko Iho, Masato Katahira, Saburo Yamamoto, Sohkichi Matsumoto

“…Mycobacterial DNA-binding protein 1 (MDP1, namely Rv2986c, hupB or HU) is a major Mycobacterium tuberculosis protein that induces vaccine-efficacy by co-administration with CpG DNA. …”
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High-parameter phenotypic characterization reveals a subset of human Th17 cells that preferentially produce IL-17 against M. tuberculosis antigen by Paul Ogongo, Paul Ogongo, Anthony Tran, Florence Marzan, David Gingrich, Melissa Krone, Francesca Aweeka, Cecilia S. Lindestam Arlehamn, Jeffrey N. Martin, Steven G. Deeks, Peter W. Hunt, Joel D. Ernst

“…BackgroundInterleukin-17–producing CD4 T cells contribute to the control of Mycobacterium tuberculosis (Mtb) infection in humans; whether infection with human immunodeficiency virus (HIV) disproportionately affects distinct Th17-cell subsets that respond to Mtb is incompletely defined.MethodsWe performed high-definition characterization of circulating Mtb-specific Th17 cells by spectral flow cytometry in people with latent TB and treated HIV (HIV-ART). …”
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Trends of Drug-Resistant Tuberculosis in an Urban and a Rural Area in China: A 10-Year Population-Based Molecular Epidemiological Study by Xu P, Li M, Jiang Q, Yang C, Liu X, Takiff H, Gao Q

“…We aimed to investigate and compare trends in TB drug resistance in the urban Songjiang District of Shanghai from 2011 to 2020, and the rural Wusheng County of Sichuan Province from 2009 to 2020, to assess the effectiveness of local TB control and treatment programs.Methods: Whole-genome sequencing data of Mycobacterium tuberculosis were used to predict drug-resistance profiles and identify genomic clusters. …”
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Phase I Trial Evaluating the Safety and Immunogenicity of Candidate TB Vaccine MVA85A, Delivered by Aerosol to Healthy <em>M.tb</em>-Infected Adults by Michael Riste, Julia L. Marshall, Iman Satti, Stephanie A. Harris, Morven Wilkie, Raquel Lopez Ramon, Danny Wright, Rachel E. Wittenberg, Samantha Vermaak, Rebecca Powell Doherty, Alison Lawrie, Christopher P. Conlon, Catherine Cosgrove, Fergus Gleeson, Marc Lipman, Paul Moss, Felicity Perrin, Martin Dedicoat, Henry Bettinson, Helen McShane

“…The trial did not show any safety concerns in the aerosol delivery of a candidate viral-vectored TB vaccine to two UK adults with <i>Mycobacterium tuberculosis</i> (<i>M.tb)</i> infection. It also systemically and mucosally demonstrated inducible immune responses following aerosol vaccination. …”
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Human leukocyte antigen-DQ risk heterodimeric haplotypes of left ventricular dysfunction in cardiac sarcoidosis: an autoimmune view of its role by Hironori Yamamoto, Yohei Miyashita, Hitoshi Minamiguchi, Kazuyoshi Hosomichi, Shohei Yoshida, Hidetaka Kioka, Haruki Shinomiya, Haruno Nagata, Kenji Onoue, Masato Kawasaki, Yuki Kuramoto, Akihiro Nomura, Yuichiro Toma, Tetsuya Watanabe, Takahisa Yamada, Yasuki Ishihara, Miho Nagata, Hisakazu Kato, Hideyuki Hakui, Yoshihiko Saito, Yoshihiro Asano, Yasushi Sakata

“…Within the peptide epitopes form the SLC25A4 and DSG2, high-homology sequences were found in Cutibacterium acnes and Mycobacterium tuberculosis, respectively. In this study, we detected the risk heterodimeric haplotypes of ventricular dysfunction in CS by searching for high-affinity HLA-class II and antigenic epitopes from candidate cardiac proteins.…”
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PatA Regulates Isoniazid Resistance by Mediating Mycolic Acid Synthesis and Controls Biofilm Formation by Affecting Lipid Synthesis in Mycobacteria by Kun Wang, Yimin Deng, Xujie Cui, Mengli Chen, Yanzhe Ou, Danting Li, Minhao Guo, Weihui Li

“…IMPORTANCE Tuberculosis, caused by Mycobacterium tuberculosis, leads to a large number of human deaths every year. …”
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Ultrasound-Assisted Synthesis of 4-Alkoxy-2-methylquinolines: An Efficient Method toward Antitubercular Drug Candidates by Ana Flávia Borsoi, Josiane Delgado Paz, Kenia Pissinate, Raoní Scheibler Rambo, Víctor Zajaczkowski Pestana, Cristiano Valim Bizarro, Luiz Augusto Basso, Pablo Machado

“…Tuberculosis (TB) has been described as a global health crisis since the second half of the 1990s. <i>Mycobacterium tuberculosis</i> (Mtb), the etiologic agent of TB in humans, is a very successful pathogen, being the main cause of death in the population among infectious agents. …”
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Methods for handling missing data in serially sampled sputum specimens for mycobacterial culture conversion calculation by Samantha Malatesta, Isabelle R. Weir, Sarah E. Weber, Tara C. Bouton, Tara Carney, Danie Theron, Bronwyn Myers, C. Robert Horsburgh, Robin M. Warren, Karen R. Jacobson, Laura F. White

“…Our primary outcome was time to culture conversion, defined as two consecutive weeks with no Mycobacterium tuberculosis growth. Methods used to address missing data included: 1) available case analysis, 2) last observation carried forward, and 3) multiple imputation by fully conditional specification. …”
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Bladder cancer-associated microbiota: Recent advances and future perspectives by Wentao Zhang, Fuhan Yang, Shiyu Mao, Ruiliang Wang, Haotian Chen, Yuefei Ran, Shenghua Liu, Pengfei Wu, Yang Yan, Wei Li, Junfeng Zhang, Xudong Yao

“…Clinicians widely recognize the role of Bacille Calmette Guérin (BCG), an attenuated Mycobacterium tuberculosis vaccine, in the management of intermediate- and high-risk NMIBC. …”
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Etiology and the challenge of diagnostic testing of community-acquired pneumonia in children and adolescents by Zulma Vanessa Rueda, Yudy Aguilar, María Angélica Maya, Lucelly López, Andrea Restrepo, Carlos Garcés, Olga Morales, Claudia Roya-Pabón, Mónica Trujillo, Catalina Arango, Ángela Rocio Copete, Cristian Vera, Margarita Rosa Giraldo, Mariana Herrera, Lázaro A. Vélez

“…Induced sputum (IS) was submitted for stains and cultures for pyogenic bacteria and Mycobacterium tuberculosis, and multiplex PCR (mPCR) for bacteria and viruses; urinary antigens for pneumococcus and Legionella pneumophila; nasopharyngeal swabs for viruses, and paired serology for atypical bacteria and viruses. …”
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An essential periplasmic protein coordinates lipid trafficking and is required for asymmetric polar growth in mycobacteria by Kuldeepkumar R Gupta, Celena M Gwin, Kathryn C Rahlwes, Kyle J Biegas, Chunyan Wang, Jin Ho Park, Jun Liu, Benjamin M Swarts, Yasu S Morita, E Hesper Rego

“…Mycobacteria, including the human pathogen Mycobacterium tuberculosis, grow by inserting new cell wall material at their poles. …”
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Elevated pulmonary tuberculosis biomarker miR-423-5p plays critical role in the occurrence of active TB by inhibiting autophagosome-lysosome fusion by Huihui Tu, Su Yang, Tingting Jiang, Liliang Wei, Liying Shi, Changming Liu, Chong Wang, Huai Huang, Yuting Hu, Zhongliang Chen, Jing Chen, Zhongjie Li, Jicheng Li

“…Moreover, although auto-lysosome is critical in clearing Mycobacterium tuberculosis, the pathological significance of microRNAs, as biomarkers of tuberculosis, in autophagosome maturation is unclear. …”
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Synthesis, characterization and anti-tubercular activities of copper(II) complexes of substituted 2,3-isatin bisthiosemicarbazones: An experimental and theoretical approach by Qurat Ul Ain, Amandeep Singh, Iqubal Singh, Raanan Carmieli, Rekha Sharma

“…The minimal binding energies calculated through molecular modeling with mycobacterium tuberculosis enoyl reductase (PDB ID: 2H7M) is −7.6 (2,3 H2bitsc), −7.3 (2,3 H2bitsc-N1-Me), −9.6 (2,3 H2bitsc-N1-Ph), −8.7 (1), −8.9 (2), and −10.7 (3) Kcal/mol. …”
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Long non-coding RNA expression in PBMCs of patients with active pulmonary tuberculosis by Guoli Li, Zhelong Feng, Honghuan Song, Yajing Wang, Limei Zhu, Yan Li, Yan Li

“…PurposeMycobacterium tuberculosis (Mtb) infection is the primary cause of the chronic infectious illness tuberculosis (TB). …”
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Active close contact investigation of tuberculosis through computer-aided detection and stool Xpert MTB/RIF among people living in Oromia Region, Ethiopia (CADOOL Study): protocol... by Francesco Di Gennaro, Annalisa Saracino, Francesco Cavallin, Giovanni Putoto, Alemseged Abdissa, Birhanu Kenate, Ademe Tsegaye, Fabio Manenti, Kidist Bobosha, Francesco Vladimiro Segala, Worku Nigussa, Giacomo Guido, Enzo Facci, Berhanu Gulo, Sergio Cotugno, Azmach Biset Asmare, Melaku Tilahun, Maddalena Miccio

“…In the referral hospital in Southwest Shoa Zone, Oromia Region, Ethiopia, patients with pulmonary TB and a sputum sample positive for Mycobacterium tuberculosis and household contacts of at least 4 years of age will be enrolled, with a target sample size of 231 patients. …”
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PD-1/PD-L1 governed cross-talk of exhausted CD8+ T and memory B cells in systemic lupus erythematosus by Fangfang Sun, Kaiwen Wang, Shuang Ye, Jia Li, Liou Cao, Shuangjun He, Jiangfeng Zhao, Xuemei Feng, Zhangling Xu, Haiting Yang, Jiaer Ye

“…Background Indeterminate readout of the quantitative interferon-γ release test (QFT) for Mycobacterium tuberculosis screening is a specific laboratory finding for systemic lupus erythematosus (SLE), which may be due to T-cell exhaustion and abnormal programmed death receptor 1 (PD-1)/programmed death-ligand 1 (PD-L1) signalling.Methods We enrolled 104 patients with SLE and 225 with other rheumatic musculoskeletal diseases (RMDs) who presented to the outpatient clinic between 2020 and 2023. …”
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Synthesis, Anticancer and Antitubercular Properties of New Chalcones and Their Nitrogen-Containing Five-Membered Heterocyclic Hybrids Bearing Sulfonamide Moiety by Lina Fernanda Castaño, Jairo Quiroga, Rodrigo Abonia, Daniel Insuasty, Oscar M. Vidal, Rosalia Seña, Vivian Rubio, Gloria Puerto, Manuel Nogueras, Justo Cobo, Juan Guzman, Alberto Insuasty, Braulio Insuasty

“…Moreover, compounds <b>20e</b> and <b>20f</b> displayed growth inhibition of <i>Mycobacterium tuberculosis</i> H37Rv at concentrations below 10 μM. …”
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Feasibility and Sensitivity of Saliva GeneXpert MTB/RIF Ultra for Tuberculosis Diagnosis in Adults in Uganda by Patrick Byanyima, Sylvia Kaswabuli, Emmanuel Musisi, Catherine Nabakiibi, Josephine Zawedde, Ingvar Sanyu, Abdul Sessolo, Alfred Andama, William Worodria, Laurence Huang, J. Lucian Davis

“…We calculated sensitivity to determine TB diagnostic performance in comparison to a composite reference standard of Mycobacterium tuberculosis liquid and solid cultures on two spot sputum specimens. …”
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New Quinoline–Urea–Benzothiazole Hybrids as Promising Antitubercular Agents: Synthesis, In Vitro Antitubercular Activity, Cytotoxicity Studies, and In Silico ADME Profiling by Rashmika Moodley, Chakes Mashaba, Goitsemodimo H. Rakodi, Nomagugu B. Ncube, Mabuatsela V. Maphoru, Mohammed O. Balogun, Audrey Jordan, Digby F. Warner, Rene Khan, Matshawandile Tukulula

“…In vitro evaluation of these hybrid compounds for their antitubercular inhibitory activity against the <i>Mycobacterium tuberculosis</i> H₃₇Rv pMSp12::GPF bioreporter strain was undertaken. …”
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COINFECÇÃO POR TUBERCULOSE PERITONEAL E HANSENIASE EM ESCOLAR: ESTUDO DE CASO by Paula Francis Gomes Viana Ribeiro, Thalita Mara de Oliveira, Marcia Hueb, Leticia Rossetto da Silva Calvacante, Maria de Lourdes Francescon

“…Introdução: A Tuberculose (TB) e a Hanseníase são causadas por micobactérias, Mycobacterium tuberculosis e Mycobacterium leprae, respectivamente. …”
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