Protein post-translational modification mimicry for cargo sorting to extracellular vesicles by Martin, C

“…To achieve functional delivery, PTTG1IP-cargo fusion constructs were designed to include self-cleaving sequences (i.e., an intein from Mycobacterium tuberculosis or an intrinsic cleavage sequence derived from PTTG1IP itself) to facilitate cargo release from the EV scaffold. …”

Development and evaluation of mucosal tuberculosis vaccine utilizing in-trans surface display system of Lactobacillus plantarum Pa21 in mice by Mustafa, Anhar Danial

“…The severity of tuberculosis is significant since it has been estimated to have latently affected almost a third of the world’s population with Mycobacterium tuberculosis, the causative agent for tuberculosis. …”
Get full text

Enhancing urinary tract infection diagnosis for negative culture patients with metagenomic next-generation sequencing (mNGS) by Kaipeng Jia, Kaipeng Jia, Shiwang Huang, Shiwang Huang, Chong Shen, Chong Shen, Hongjun Li, Zhe Zhang, Zhe Zhang, Lei Wang, Gangjian Zhao, Gangjian Zhao, Zhouliang Wu, Zhouliang Wu, Yuda Lin, Yuda Lin, Han Xia, Mingze Tang, Huifen Yang, Hailong Hu, Hailong Hu

“…All anaerobic bacteria, Mycobacterium Tuberculosis and all mixed pathogens were detected by mNGS. …”
Get full text

A stool based qPCR for the diagnosis of TB in children and people living with HIV in Uganda, Eswatini and Mozambique (Stool4TB): a protocol for a multicenter diagnostic evaluation by Lucia Carratala-Castro, Willy Ssengooba, Alex Kay, Sozinho Acácio, Joanna Ehrlich, Andrew R DiNardo, Nosisa Shiba, Joachim K Nsubuga, Shilzia Munguambe, Belén Saavedra-Cervera, Patricia Manjate, Durbbin Mulengwa, Busizwe Sibandze, Mangaliso Ziyane, George Kasule, Edson Mambuque, Moorine Penninah Sekadde, Eric Wobudeya, Moses L Joloba, Jan Heyckendorf, Christoph Lange, Sabine Hermans, Anna Mandalakas, Alberto L. García-Basteiro, Elisa Lopez-Varela, on behalf of Stool4TB Global Partnership

“…To obtain bacteriological confirmation, participants will provide respiratory samples (1 for adults, 2 in children) and 1 stool sample for Xpert Ultra MTB/RIF (Cepheid, Sunnyvale, CA, USA). Mycobacterium tuberculosis (M.tb) liquid culture will only be performed in respiratory samples and lateral flow lipoarabinomannan (LF-LAM) in urine following WHO recommendations. …”
Get full text

New Phocoenamicin and Maklamicin Analogues from Cultures of Three Marine-Derived <i>Micromonospora</i> Strains by Maria Kokkini, Daniel Oves-Costales, Pilar Sánchez, Ángeles Melguizo, Thomas A. Mackenzie, Mercedes Pérez-Bonilla, Jesús Martín, Arianna Giusti, Peter de Witte, Francisca Vicente, Olga Genilloud, Fernando Reyes

“…All the isolated compounds were tested against various human pathogens and revealed diverse strong to negligible activity against methicillin-resistant <i>Staphylococcus aureus</i>, <i>Mycobacterium tuberculosis</i> H37Ra, <i>Enterococcus faecium</i> and <i>Enterococcus faecalis</i>. …”
Get full text

Pathogen Identification in Suspected Cases of Pyogenic Spondylodiscitis by Ahmad Farajzadeh Sheikh, Ahmad Farajzadeh Sheikh, Azar D. Khosravi, Azar D. Khosravi, Hamed Goodarzi, Hamed Goodarzi, Hamed Goodarzi, Roohangiz Nashibi, Roohangiz Nashibi, Alireaza Teimouri, Alireaza Teimouri, Azim Motamedfar, Reza Ranjbar, Sara Afzalzadeh, Mehrandokht Cyrus, Mohammad Hashemzadeh, Mohammad Hashemzadeh

“…Sanger sequencing of the PCR products identified the following bacteriological agents: Mycobacterium tuberculosis (n = 9; 42.9%), Staphylococcus aureus (n = 6; 28.5%), Mycobacterium abscessus (n = 5; 23.8%), and Mycobacterium chelonae (n = 1; 4.8%). 36 samples displayed no visible 16S rRNA PCR signal, which suggested that non-bacterial infectious agents (e.g., fungi) or non-infectious processes (e.g., inflammatory, or neoplastic) may be responsible for some of these cases. …”
Get full text

Anti-PD-1 immunotherapy leads to tuberculosis reactivation via dysregulation of TNF-α by Liku B Tezera, Magdalena K Bielecka, Paul Ogongo, Naomi F Walker, Matthew Ellis, Diana J Garay-Baquero, Kristian Thomas, Michaela T Reichmann, David A Johnston, Katalin Andrea Wilkinson, Mohamed Ahmed, Sanjay Jogai, Suwan N Jayasinghe, Robert J Wilkinson, Salah Mansour, Gareth J Thomas, Christian H Ottensmeier, Alasdair Leslie, Paul T Elkington

“…In patients, PD-1 is expressed in Mycobacterium tuberculosis (Mtb)-infected lung tissue but is absent in areas of immunopathology. …”
Get full text

RECIDIVA DE TUBERCULOSE PULMONAR EM ADULTOS EM UM ESTADO DO SUL DO BRASIL by Ana Beatriz Floriano de Souza, Maria de Fátima Oliveira Hirth Ruiz, Rejane Kiyomi Furuya, Vanessa Cristina Luquini, Camila dos Santos Peres, Erick Souza Neri, Luana Graziely Parra da Silva, Giovanna Yamashita Tomita, Natalia Marciano de Araujo Ferreira, Andressa Midori Sakai, Tissiane Soares Seixas de Mattos, Ana Caroline Carvalho, Flávia Meneguetti Pieri

“…Introdução/objetivo: A forma pulmonar é manifestação mais comum e de maior repercussão de saúde pública da tuberculose, causada pelo Mycobacterium tuberculosis. A recidiva de tuberculose ocorre quando uma pessoa, que já teve a doença ativa previamente e recebeu alta após comprovação de cura ou conclusão do tratamento, volta a manifestá-la, seja por reativação endógena do bacilo ou por reinfecção exógena. …”
Get full text

Comprehensive assessment of invalid and indeterminate results in Truenat MTB-RIF testing across sites under the national TB elimination program of India by Radha Gopalaswamy, Nishant Kumar, Himanshu Vashistha, Priya Rajendran, Jyoti Kayesth, Carel Joseph Peravali, Satabdi Kashyap, Shreeparna Ghosh, Habakkuk Yumo, Moe Moore, Sridhar Anand, Ranjani Ramachandran, Umesh Alavadi, Sanjeev Saini, Sivakumar Shanmugam

“…IntroductionTruenat MTB-RIF assay (Truenat), a nucleic acid amplification test (NAAT), is a real-time polymerase chain reaction (RT-PCR) chip-based assay that can detect Mycobacterium tuberculosis (Mtb) and rifampicin (RIF) drug resistance using portable, battery-operated devices. …”
Get full text

Annual short-burst mass anthelmintic administration reduces tuberculosis severity but not prevalence in a wildlife reservoir by Joanne Lello, Diana Gassó, Pilar Gonçalves, David Risco, David Risco, Waldo L. García, Joaquim Segalés, Joaquim Segalés, Cristina Garrido-Amaro, Gregorio Mentaberre, Irene Torres-Blas, Roser Velarde, Jorge R. López-Olvera, Pedro Fernández-Llario, Emmanuel Serrano

“…IntroductionTuberculosis (TB), caused by the Mycobacterium tuberculosis complex (MTC), is an important disease in both human and animal systems. …”
Get full text

Performance of two interferon-gamma release assays for tuberculosis infection screening in Kawasaki children before immunosuppressive therapy by Hao Chen, Huiwen Zheng, Lang Cui, Jing Xiao, Feina Li, Yonghong Wang, Yajie Guo, Yuying Chen, Yue Yuan, Chen Shen

“…ObjectiveWe aimed to compare QuantiFERON-TB Gold In-Tube (QFT-GIT) and X.DOT-TB for screening latent tuberculosis infection (LTBI) in kawasaki patients, and to identify the risk factors associated with indeterminate IGRA results.MethodsWe conducted a retrospective study on children with KD, who were screened for mycobacterium tuberculosis (Mtb) infection by either ELISA-based QFT-GIT or ELISPOT-based X.DOT-TB tests, admitted in Department of Cardiology, Beijing Children's Hospital from July 2019 to April 2022.ResultsA total of 1327 cases were included. …”
Get full text

Predominance of Escherichia-Shigella in Gut Microbiome and Its Potential Correlation with Elevated Level of Plasma Tumor Necrosis Factor Alpha in Patients with Tuberculous Meningit... by Shanshan Li, Jidong Guo, Rongmei Liu, Fuzhen Zhang, Shu’an Wen, Yi Liu, Weicong Ren, Xuxia Zhang, Yuanyuan Shang, Mengqiu Gao, Jie Lu, Yu Pang

“…Thereafter, cells of commensal Escherichia coli (E. coli) were isolated and administered to model mice by gavage and then mice were inoculated with Mycobacterium tuberculosis (M. tuberculosis). Subsequently, these mice exhibited increased blood TNF-α levels accompanied by downregulated expression of tight junction protein claudin-5, increased brain tissue bacterial burden, and elevated central nervous system inflammation relative to corresponding indicators in controls administered PBS by gavage. …”
Get full text

Host-directed therapies in pulmonary tuberculosis: Updates on anti-inflammatory drugs by Juan M. Cubillos-Angulo, Juan M. Cubillos-Angulo, Juan M. Cubillos-Angulo, Betânia M. F. Nogueira, Betânia M. F. Nogueira, María B. Arriaga, María B. Arriaga, María B. Arriaga, Beatriz Barreto-Duarte, Beatriz Barreto-Duarte, Beatriz Barreto-Duarte, Beatriz Barreto-Duarte, Mariana Araújo-Pereira, Mariana Araújo-Pereira, Mariana Araújo-Pereira, Catarina D. Fernandes, Caian L. Vinhaes, Caian L. Vinhaes, Caian L. Vinhaes, Klauss Villalva-Serra, Klauss Villalva-Serra, Klauss Villalva-Serra, Klauss Villalva-Serra, Vanessa M. Nunes, João P. Miguez-Pinto, Eduardo P. Amaral, Bruno B. Andrade, Bruno B. Andrade, Bruno B. Andrade, Bruno B. Andrade, Bruno B. Andrade

“…Adjunct host-directed therapy (HDT) is an emerging strategy in TB treatment that aims to target the host immune response to Mycobacterium tuberculosis in addition to antimycobacterial drugs. …”
Get full text

BCG-Based Vaccines Elicit Antigen-Specific Adaptive and Trained Immunity against SARS-CoV-2 and <i>Andes orthohantavirus</i> by Jorge A. Soto, Fabián E. Díaz, Angello Retamal-Díaz, Nicolás M. S. Gálvez, Felipe Melo-González, Alejandro Piña-Iturbe, Mario A. Ramírez, Karen Bohmwald, Pablo A. González, Susan M. Bueno, Alexis M. Kalergis

“…<b>Background:</b><i>Mycobacterium bovis</i> Bacillus Calmette-Guérin (BCG) is a live attenuated vaccine mainly administered to newborns and used for over 100 years to prevent the disease caused by <i>Mycobacterium tuberculosis</i> (<i>M. tb</i>). This vaccine can induce immune response polarization towards a Th1 profile, which is desired for counteracting <i>M. tb</i>, other mycobacteria, and unrelated intracellular pathogens. …”
Get full text

Comparing Current and Next-Generation Humanized Mouse Models for Advancing HIV and HIV/<i>Mtb</i> Co-Infection Studies by Madeleine Lepard, Jack X. Yang, Sam Afkhami, Aisha Nazli, Anna Zganiacz, Shangguo Tang, Margaret Wa Yan Choi, Fatemah Vahedi, Alexandre Deshiere, Michel J. Tremblay, Zhou Xing, Charu Kaushic, Amy Gillgrass

“…In people living with HIV, <i>Mycobacterium tuberculosis</i> (<i>Mtb</i>) is the major cause of death. …”
Get full text

Tuberculosis alters immune-metabolic pathways resulting in perturbed IL-1 responses by Alba Llibre, Nikaïa Smith, Vincent Rouilly, Munyaradzi Musvosvi, Elisa Nemes, Céline Posseme, Simbarashe Mabwe, Bruno Charbit, Stanley Kimbung Mbandi, Elizabeth Filander, Hadn Africa, Violaine Saint-André, Violaine Saint-André, Vincent Bondet, Pierre Bost, Pierre Bost, Humphrey Mulenga, Nicole Bilek, Matthew L. Albert, Thomas J. Scriba, Darragh Duffy, Darragh Duffy

“…Tuberculosis (TB) remains a major public health problem and we lack a comprehensive understanding of how Mycobacterium tuberculosis (M. tb) infection impacts host immune responses. …”
Get full text

AVALIAÇÃO DA ASSOCIAÇÃO ENTRE VARIANTES GENÉTICAS DO RECEPTOR TOLL-LIKE 4 COM O RISCO DE DESENVOLVIMENTO DA TUBERCULOSE PULMONAR by Romário Martins Araújo, Wlisses Henrique Veloso de Carvalho Silva, Kessia Kelly Batista da Silva, Nathyeli Oliveira do Nascimento, Josefa Nayara dos Santos Nascimento, Bárbara Wanessa Delgado Abrantes, Renata Inglez de Souza Tejo, Rayssa Maria Pastick Jares da Costa, Danielle Martiniano da Silva Rodrigues, Milena Brandão de Lima, Jéssica Lopes Teixeira, Lilian Maria Lapa Montenegro, Haiana Charifker Schindler

“…Introdução/objetivo: A tuberculose (TB) é uma doença causada pelo Mycobacterium tuberculosis (Mtb) que permanece como uma das principais causas de doença e morte na população. …”
Get full text

A Single-Run HPLC–MS Multiplex Assay for Therapeutic Drug Monitoring of Relevant First- and Second-Line Antibiotics in the Treatment of Drug-Resistant Tuberculosis by Niklas Köhler, Hande Karaköse, Hans-Peter Grobbel, Doris Hillemann, Sönke Andres, Christina König, Barbara Kalsdorf, Thomas Theo Brehm, Laura Böttcher, Inna Friesen, Harald Hoffmann, Dražen Strelec, Dagmar Schaub, Charles A. Peloquin, Stefan Schmiedel, Laurent A. Decosterd, Eva Choong, Sebastian G. Wicha, Rob E. Aarnoutse, Christoph Lange, Patricia M. Sánchez Carballo

“…The treatment of drug-resistant <i>Mycobacterium tuberculosis</i> relies on complex antibiotic therapy. …”
Get full text

Mechanochemical Studies on Coupling of Hydrazines and Hydrazine Amides with Phenolic and Furanyl Aldehydes—Hydrazones with Antileishmanial and Antibacterial Activities by Anna Kapusterynska, Christian Bijani, Damian Paliwoda, Laure Vendier, Valérie Bourdon, Nicolas Imbert, Sandrine Cojean, Philippe Marie Loiseau, Deborah Recchia, Viola Camilla Scoffone, Giulia Degiacomi, Abdul Akhir, Deepanshi Saxena, Sidharth Chopra, Vira Lubenets, Michel Baltas

“…Four compounds (<b>1</b>, <b>3</b>, <b>5</b> and <b>8</b>) showed good activity against <i>Mycobacterium tuberculosis</i>, and one (<b>7</b>) was very potent against <i>Staphylococcus aureus</i>. …”
Get full text

Performance of Xpert MTB/RIF and sputum microscopy compared to sputum culture for diagnosis of tuberculosis in seven hospitals in Indonesia by Anis Karuniawati, Erlina Burhan, Eko Budi Koendhori, Desvita Sari, Budi Haryanto, Titik Nuryastuti, A. A. A. Yuli Gayatri, Uleng Bahrun, R. Lia Kusumawati, Retna Indah Sugiyono, Nugroho Harry Susanto, Aly Diana, Aly Diana, Herman Kosasih, Adhella Menur Naysilla, Dewi Lokida, Aaron Neal, Sophia Siddiqui, Chuen-Yen Lau, Muhammad Karyana

“…We evaluated sensitivity and specificity of AFB smear and Xpert MTB/RIF using Mycobacterium tuberculosis (Mtb) culture as the gold standard, factors associated with assay results, and consistency of Xpert MTB/RIF with drug susceptibility test (DST) in detecting rifampicin resistance.ResultsSensitivity of AFB smear was significantly lower than Xpert MTB/RIF (86.2 vs. 97.4%, p-value &lt;0.001), but specificity was significantly better (86.7 vs. 73.3%, p-value &lt;0.001). …”
Get full text