Enhanced biocatalytic esterification with lipase-immobilized chitosan/graphene oxide beads by Lau, Siaw Cheng, Lim, Hong Ngee, Basri, Mahiran, Masoumi, Hamid Reza Fard, Ahmad Tajudin, Asilah, Huang, Nay Ming, Pandikumar, Alagarsamy, Chia, Chi Hua, Andou, Yoshito

“…The lipase immobilization on the beads involved the functionalization with coupling agents, N-hydroxysulfosuccinimide sodium (NHS) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide (EDC), and it possessed a high enzyme activity of 64 U. …”
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Preparation Of Conjugated Thermo-Sensitive Poly (Nipam) For Affinity Precipitation Of Enzyme by Syaubari, Syaubari

“…The effect of molecular weight, reaction time, ligand PABA load, pH condition and addition of NHS on conjugated PABA-poly (NIPAM) were examined including the amount of ligand conjugated and dried weight precipitate of polymer. …”
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Electrochemical immunosensor for detection of aflatoxin B1 based on indirect competitive ELISA by Azri, Farah Asilah, Sukor, Rashidah, Selamat, Jinap, Abu Bakar, Fatimah, Yusof, Nor Azah, Hajian, Reza

“…The optimized assay was transferred onto the multi-walled carbon nanotubes/chitosan/screen-printed carbon electrode (MWCNTs/CS/SPCE) by covalent attachment with the aid of 1-Ethyl-3-(3-dimetylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS). Competition occurred between aflatoxin B1-bovine serum albumin (AFB1–BSA) and free AFB1 (in peanut sample and standard) for the binding site of a fixed amount of anti-AFB1 antibody. …”
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Synthesis and in vitro visualization of fluorescein isothiocyanate-labeled chitosan nanoparticles (fitc-cnp) in human kidney cancer cells by Tay, Andrina Chu Huey

“…CNPs were prepared using a modified ionic gelation method to yield spherical nanoparticles, and fluorescent labeling of CNP was performed using N-hydroxy-succinimidyl (NHS)-FITC. Particle size distribution and polydispersity index of nanoparticles were analyzed using dynamic light scattering. …”
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Improving National and Homeland Security through a proposed Laboratory for Information Globalization and Harmonization Technologies (LIGHT) by Choucri, Nazli, Madnick, Stuart E., Siegel, Michael, Wang, Richard

“…Improved access and use of information are essential to better identify and anticipate threats, protect against and respond to threats, and enhance national and homeland security (NHS), as well as other national priority areas, such as Economic Prosperity and a Vibrant Civil Society (ECS) and Advances in Science and Engineering (ASE). …”
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TDSF: Two-phase tamper detection in semi-fragile watermarking using two-level integer wavelet transform by Amrullah, Agit, Ernawan, Ferda, Anis Farihan, Mat Raffei, Liew, Siau-Chuin

“…This study also used Normalized Hamming Similarity (NHS) to classify malicious and incidental attacks. …”
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Altered Aortic Hemodynamics and Relative Pressure in Patients with Dilated Cardiomyopathy by Marlevi, David

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Genetically Engineered Alginate Lyase-PEG Conjugates Exhibit Enhanced Catalytic Function and Reduced Immunoreactivity by Lamppa, John W., Ackerman, Margaret E., Lai, Jennifer I., Scanlon, Thomas C., Griswold, Karl E.

“…In contrast to random PEGylation of the enzyme by NHS-ester mediated chemistry, controlled mono-PEGylation of A1-III alginate lyase produced a conjugate that maintained wild type levels of activity towards a model substrate. …”
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Immobilized peptide asparaginyl ligases enhance stability and facilitate macrocyclization and site-specific ligation by Hemu, Xinya, To, Janet, Zhang, Xiaohong, Tam, James P.

“…We compared immobilization indirectly via noncovalent affinity capture using NeutrAvidin or concanavalin A agarose beads or directly via covalent coupling of free amines on the enzyme surface with the N-hydroxysuccinimide (NHS) ester attached on agarose beads. The catalytic efficiency of immobilized PALs correlated with the distance between the biocatalysts and the solid supports, and in turn, the mobility of enzymes and the accessibility of substrates. …”
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Gold nanorods surface modification and bioconjugation for label-free biosensor by Ng, Shu Rui.

“…Next, MPA containing the carboxylic group was introduced mainly onto the ends of the GNRs, giving PEG/MPA-GNRs. Following EDC/NHS activation, anti-rabbit IgG was bioconjugated onto the MPA molecules present on the PEG/MPA-GNRs. …”
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Fabrication and characterization of targeting liposomes for nanomedicine applications by Yeo, Edmund Wei Liang

“…Conjugation of targeting peptide to liposome was first performed using EDC, Sulfo-NHS activation methodology. Conjugated DPPC-COOH-Peptide 1 liposome with a size of 152.7nm, stable zeta potential of -37.7mV and conjugation efficiency of 70.2% (HPLC analysis) was identified as the lead candidate for atherosclerosis drug delivery. …”
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Peptide-induced formation of a tethered lipid bilayer membrane on mesoporous silica by Wallin, Maria, Choi, Jae‑Hyeok, Kim, Seong Oh, Cho, Nam‑Joon, Andersson, Martin

“…The purpose of this work was to use these properties to fabricate and characterize a zwitterionic 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine lipid tBLM containing 2 mol % 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-maleimide(poly(ethylene glycol))-2000 (DSPE-PEG2000-NHS) lipid tethers on a 3-aminopropyltrimethoxysilane-modified mesoporous silica substrate. …”
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An exploration of adult male experiences of having irritable bowel syndrome (IBS) : a qualitative study by Campbell, Nigel

“…IBS places much demand on the NHS to fund and treat. It is twice as common for IBS to be found in women compared to men. …”
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Evaluation of phosphate knowledge and the effectiveness of a phosphate management protocol to achieve optimum serum phosphate levels in haemodialysis patients by Yokum, Dawn

“…A randomised, controlled trial was carried out at haemodialysis units at Barts and the London NHS Trusts and satellite units. The project followed thirty-four clinically stable adults undergoing regular haemodialysis with a serum phosphate level > 1.8mmol/l on at least one occasion within 4 months of starting the study. …”
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Non-invasive control of protein-surface interactions for repeated electrochemical immunosensor use by Khoo, M.M., Rahim, Z., Darwish, N.T., Alias, Y., Khor, S.M.

“…A reusable electrochemical immunosensor was developed on which a glassy carbon plate was passivated with a mixed layer of 4-nitroaniline/p-phenylenediamine, attached with gold nanoparticles, followed by surface-bound epitope (sulfo-NHS-biotin) to which the monoclonal anti-biotin IgG antibody was complexed. …”
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PNA biosensor based on reduced graphene oxide/water soluble quantum dots for the detection of Mycobacterium tuberculosis by Mat Zaid, Mohd Hazani, Abdullah, Jaafar, Yusof, Nor Azah, Sulaiman, Yusran, Wasoh, Helmi, Md Noh, Mohd Fairulnizal, Issa, Rahizan

“…Firstly, NH2-GO/QDs was applied onto screen-printed carbon electrode (SPCE) surface by electro-deposition method, then the PNA probe was immobilized onto the surface of NH2-GO/QDs modified SPCE via EDC/NHS coupling technique. Subsequently, the developed PNA biosensor was used to hybridize with target DNA. …”
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Development of sandwich and label-free electrochemical immunosensor for detection of HER2 breast cancer biomarker by Lah, Zur Mira Azizah @ Nor Haiza

“…The fabrication of label-free immunosensor involved several steps; (1) pre-treatment of screen printed carbon electrode (SPCE) (2) the binding of primary anti-HER2 antibody to pre-treated SPCE via EDC/NHS (3) bovine serum albumin (BSA) blocking (4) the binding of HER2 antigen (5) electroanalytical performance of the label-free immunosensor. …”
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DNA electrochemical biosensor based on iron oxide/nanocellulose crystalline composite modified screen-printed carbon electrode for detection of Mycobacterium tuberculosis by Mat Zaid, Mohd Hazani, Che-Engku-Chik, Che Engku Noramalina, Yusof, Nor Azah, Abdullah, Jaafar, Othman, Siti Sarah, Issa, Rahizan, Md Noh, Mohd Fairulnizal, Wasoh, Helmi

“…Then, a specific sequence of MTB DNA probe was immobilized onto a modified SPCE surface by using the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling mechanism. For better signal amplification and electrochemical response, ruthenium bipyridyl Ru(bpy)32+ was assigned as labels of hybridization followed by the characteristic test using differential pulse voltammetry (DPV). …”
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Fabrication and Cell Responsive Behavior of Macroporous PLLA/Gelatin Composite Scaffold with Hierarchical Micro-Nano Pore Structure by Song, Kedong, Ji, Lili, Zhang, Jingying, Wang, Hai, Jiao, Zeren, Mayasari, Lim, Fu, Xiaoyan, Liu, Tianqing

“…The scaffolds with mild hydrolysis by NaOH were modified by 1-ethyl-3-(3-dimethyl ami-nopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS). Gelatin was performed onto PLLA scaffold via TIPS aiming at enhancement cell-material interaction. …”
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Biomimetic photonic coatings using squid reflectin protein by Loke, Jun Jie

“…This thesis describes a systematic approach to self-assemble SlRF-B1 into discrete nanoparticle sizes when conjugated with click-chemistry ligand dibenzocyclooctyne (DBCO)-sulfo-NHS ester. This was achieved by varying the solvent conditions during the self-assembly process. …”
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