NRK1 controls nicotinamide mononucleotide and nicotinamide riboside metabolism in mammalian cells by Joanna Ratajczak, Magali Joffraud, Samuel A. J. Trammell, Rosa Ras, Núria Canela, Marie Boutant, Sameer S. Kulkarni, Marcelo Rodrigues, Philip Redpath, Marie E. Migaud, Johan Auwerx, Oscar Yanes, Charles Brenner, Carles Cantó

“…Here, Ratajczak et al. show that cellular levels of NAD+ depend on the extracellular catalytic activity of NRK1, which processes two NAD+precursors, nicotinamide mononucleotide and nicotinamide riboside, in mice.…”
Get full text

Nicotinamide riboside kinase 1 protects against diet and age-induced pancreatic β-cell failure by Angelique Cercillieux, Joanna Ratajczak, Magali Joffraud, José Luis Sanchez-Garcia, Guillaume Jacot, Alix Zollinger, Sylviane Métairon, Judith Giroud-Gerbetant, Marie Rumpler, Eleonora Ciarlo, Miriam Valera-Alberni, Audrey Sambeat, Carles Canto

Subjects: “…Nicotinamide riboside kinase 1 (NRK1)…”
Get full text

A Dual-Bacterial Coupled Fermentation Strategy for Nicotinamide Mononucleotide Synthesis by SUN Ting, ZHANG Hongtao, YANG Feng, CHAI Wengang, XUE Haoyang, TAN Shuyin

“…First, engineering strains expressing NRK1 and NRK2 were constructed, and the highly active Escherichia coli BL21 (DE3)-pET28a-NRK1 was selected, with NMN yield and productivity of 5.17 g/L and 77.4%, respectively. …”
Get full text

Heterologous Expression and Enzymatic Properties of Nicotinamide Nucleoside Kinase from Saccharomyces cerevisiae by HE Jianju, LIU Xinxin, SHI Hongling, RAN Luni, WANG Xian, ZHANG Yingjun, TANG Cunduo

“…In this study, the nicotinamide riboside kinase 1 gene (ScNRK1) was cloned from Saccharomyces cerevisiae, and its soluble expression in Escherichia coli was achieved using pET28a plasmid. …”
Get full text

Nicotinamide riboside kinases display redundancy in mediating nicotinamide mononucleotide and nicotinamide riboside metabolism in skeletal muscle cells by Rachel S. Fletcher, Joanna Ratajczak, Craig L. Doig, Lucy A. Oakey, Rebecca Callingham, Gabriella Da Silva Xavier, Antje Garten, Yasir S. Elhassan, Philip Redpath, Marie E. Migaud, Andrew Philp, Charles Brenner, Carles Canto, Gareth G. Lavery

“…NRK1, NRK2, and double KO myotubes revealed redundancy in the NRK dependent metabolism of NR to NAD+. …”
Get full text

High level expression of nicotinamide nucleoside kinase from Saccharomyces cerevisiae and its purification and immobilization by one-step method by Jian-Ju He, Jian-Ju He, Xin-Xin Liu, Xin-Xin Liu, Ying Li, Ying Li, Zhe Wang, Zhe Wang, Hong-Ling Shi, Hong-Ling Shi, Yun-Chao Kan, Lun-Guang Yao, Lun-Guang Yao, Cun-Duo Tang, Cun-Duo Tang

“…Then, the reScNRK1 was immobilized by metal affinity label to optimize the enzyme performance. …”
Get full text

Engler i salig blanding by Knut Lundby

“…Allmennkanalen NRK1 knytter programmer om religion og religiøsitet til den kristne kalenderen, og til kirken og dens etablerte plass i det norske samfunnet. …”
Get full text

Endogenous nicotinamide riboside metabolism protects against diet-induced liver damage by Audrey Sambeat, Joanna Ratajczak, Magali Joffraud, José L. Sanchez-Garcia, Maria P. Giner, Armand Valsesia, Judith Giroud-Gerbetant, Miriam Valera-Alberni, Angelique Cercillieux, Marie Boutant, Sameer S. Kulkarni, Sofia Moco, Carles Canto

“…Here the authors provide insight into the role of endogenous NAD+ metabolism by studying nicotinamide riboside kinase 1 (NRK1) deficient mice.…”
Get full text

Nicotinamide riboside kinases regulate skeletal muscle fiber-type specification and are rate-limiting for metabolic adaptations during regeneration by Tanja Sonntag, Tanja Sonntag, Sara Ancel, Sara Ancel, Sonia Karaz, Paulina Cichosz, Guillaume Jacot, Maria Pilar Giner, José Luis Sanchez-Garcia, Alice Pannérec, Sofia Moco, Vincenzo Sorrentino, Carles Cantó, Carles Cantó, Jérôme N. Feige, Jérôme N. Feige

“…Altogether, we demonstrate that NAD+ synthesis controls mitochondrial metabolism and fiber type composition via NRK1/2 and is rate-limiting for myogenic commitment and mitochondrial maturation during skeletal muscle repair.…”
Get full text

A reduced form of nicotinamide riboside defines a new path for NAD+ biosynthesis and acts as an orally bioavailable NAD+ precursor by Judith Giroud-Gerbetant, Magali Joffraud, Maria Pilar Giner, Angelique Cercillieux, Simona Bartova, Mikhail V. Makarov, Rubén Zapata-Pérez, José L. Sánchez-García, Riekelt H. Houtkooper, Marie E. Migaud, Sofia Moco, Carles Canto

“…Despite the minor structural difference, we found that NRH uses different steps and enzymes to synthesize NAD+, thus revealing a new NRK1-independent pathway for NAD+ synthesis. Finally, we provide evidence that NRH is orally bioavailable in mice and prevents cisplatin-induced acute kidney injury. …”
Get full text