An improved collagen zymography approach for evaluating the collagenases MMP-1, MMP-8, and MMP-13 by Seniz Inanc, Didem Keles, Gulgun Oktay

“…To validate our improved method, we investigated MMP-1 activity levels in human thyroid cancer (8505C) and normal thyroid (Nthy-ori-3–1) cell lines, finding that the proenzyme and active MMP-1 levels were greater in 8505C cells than in Nthy-ori-3–1 cells. …”
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miR-197-3p inhibits proliferation, migration and invasion of thyroid cancer cell line through regulating DMBT1 by CAO Deng-han, CHEN Kui-sheng

“…Results Compared with Nthy-ori 3-1 cells, the relative expression of miR-197-3p was increased in SW579 and CGTHW-1 cells (P＜0.05). …”
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Virologic and immunologic evidence supporting an association between HHV-6 and Hashimoto's thyroiditis. by Elisabetta Caselli, Maria Chiara Zatelli, Roberta Rizzo, Sabrina Benedetti, Debora Martorelli, Giorgio Trasforini, Enzo Cassai, Ettore C degli Uberti, Dario Di Luca, Riccardo Dolcetti

“…Furthermore, HHV-6-infected Nthy-ori3-1 cells become targets for NK-mediated killing, NK cells from HT patients show a significantly more efficient killing of HHV-6 infected thyroid cells than healthy controls, and HT patients have increased T-cell responses to HHV-6 U94 protein, associated to viral latency. …”
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Deciphering an isolated lung phenotype of NKX2-1 frameshift pathogenic variant by Céline Delestrain, Céline Delestrain, Abdel Aissat, Abdel Aissat, Elodie Nattes, Elodie Nattes, Elodie Nattes, Isabelle Gibertini, Valérie Lacroze, Stéphanie Simon, Xavier Decrouy, Alix de Becdelièvre, Alix de Becdelièvre, Pascale Fanen, Pascale Fanen, Ralph Epaud, Ralph Epaud

“…Subcellular localization was assessed by confocal microscopy in A549 and Nthy cells. Transactivation of SFTPA, SFTPB, SFTPC, and ABCA3 promoters was assessed in A549 cells. …”
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Pediatric suppurative parotitis in Cambodia between 2007 and 2011. by Stoesser, N, Pocock, J, Moore, C, Soeng, S, Chhat, H, Sar, P, Limmathurotsakul, D, Day, N, Thy, V, Sar, V, Parry, C

AXL, along with PROS1, is overexpressed in papillary thyroid carcinoma and regulates its biological behaviour by Mingze Wei, Yizeng Wang, Yuanchao Liu, Dongyang Li, Xianghui He

“…Results PROS1 and AXL were expressed in TPC-1, B-CPAP, and Nthy-Ori 3–1 cells at different levels. Expression was significantly higher in PTC cell lines (TPC-1 and B-CPAP) than in the normal thyroid cell line (Nthy-Ori 3–1) (p < 0.05). …”
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miR-205 inhibits cell proliferation of papillary thyroid carcinoma by regulating Wnt-5a by LIU Ya-qi, ZHENG Cheng-hong

“…Expression of miR-205 in NTHY-OR-3-1 was significantly lower than that of K1 and BCPAP. miR-205 inhibited the proliferation of PTC cells. …”
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Pediatric suppurative parotitis in cambodia between 2007 and 2011 by Stoesser, N, Pocock, J, Moore, C, Soeng, S, Chhat, H, Sar, P, Limmathurotsakul, D, Day, N, Thy, V, Sar, V, Parry, C

miR-135b-3p regulates XAF1 expression and affects migration, invasion and radio sensitization of thyroid cancer cells by CAO Deng-han, CHEN Kui-sheng

“…Methods Normal thyroid cells Nthy-ori 3-1, thyroid cancer cells K1 and TPC-1 were cultured. …”
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Intracellular cGMP increase is not involved in thyroid cancer cell death. by Sara D' Alessandro, Elia Paradiso, Clara Lazzaretti, Samantha Sperduti, Carmela Perri, Francesco Antoniani, Sara Righi, Manuela Simoni, Giulia Brigante, Livio Casarini

“…<h4>Materials and methods</h4>We used malignant (K1) and benign (Nthy-ori 3-1) thyroid cell lines, as well as the COS7 cells as a reference model. …”
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Downregulation of LPAR1 Promotes Invasive Behavior in Papillary Thyroid Carcinoma Cells by Zahra Bokaii Hosseini, Fatemeh Rajabi, Reza Morovatshoar, Mahshad Ashrafpour, Panteha Behboodi, Dorsa Zareie, Mohammad Natami

“…To assess the expression of LPAR1, we extracted total RNA from both the BCPAP cell line and the normal human thyroid epithelial cell line Nthy-ori 3-1. The levels of LPAR1 expression were then measured using quantitative real-time polymerase chain reaction (qRT-PCR) in the BCPAP cell line, with a comparison to the Nthy-ori 3-1 cell line. …”
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Intracellular cGMP increase is not involved in thyroid cancer cell death by Sara D’ Alessandro, Elia Paradiso, Clara Lazzaretti, Samantha Sperduti, Carmela Perri, Francesco Antoniani, Sara Righi, Manuela Simoni, Giulia Brigante, Livio Casarini

“…<h4>Materials and methods</h4> We used malignant (K1) and benign (Nthy-ori 3–1) thyroid cell lines, as well as the COS7 cells as a reference model. …”
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High concentration of estradiol has a negative correlation with free thyroxine during the second trimester of pregnancy by Leqi He, Xiaoying Li, Zaoping Chen, Wei Wang, Kai Wang, Xinmei Huang, Qian Yang, Wencai Ke, Jun Liu, Bingbing Zha

“…Multiple linear regression analysis was performed between E2 and thyroid function. The human thyroid Nthy-ori3-1 cells were cultured in different E2 concentrations, and the mRNA levels of NIS, estrogen receptor (ER)-α, and ER-β were measured by quantitative real-time PCR. …”
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Resveratrol enhances anticancer effects of paclitaxel in HepG2 human liver cancer cells by Qin Jiang, Manyi Yang, Zhan Qu, Jixiang Zhou, Qi Zhang

“…Results The 10 μg/mL of Res had no growth inhibitory effect on Nthy-ori 3–1 normal cells or HepG2 cancer cells meanwhile the 5 or 10 μg/mL of PA also had no growth inhibitory effect on Nthy-ori 3–1 normal cells. …”
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PES1上调ERα/ERβ蛋白比率促进甲状腺乳头状癌细胞增殖和侵袭迁移 by 程龙, 邱伊波, 许琳婉, 刘智敏

“…方法用免疫组化法检测PES1、ERα和ERβ在正常甲状腺组织以及PTC组织中的表达情况；用Western blot法分别检测正常甲状腺细胞株Nthy-ori3-1和PTC细胞株BCPAP和K1中PES1、ERα 和 ERβ的表达情况，以及PES1过表达、PES1下调时对Nthy-ori3-1细胞和BCPAP细胞中ERα、ERβ蛋白表达比率的影响；用MTT以及Transwell小室法检测PES1、ERα和ERβ对PTC细胞增殖、侵袭和迁移的影响。…”
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ALKBH5, an m6A demethylase, attenuates tumor growth and inhibits metastasis in papillary thyroid carcinoma by Yong Zhuang, Qingyan Cai, Xin Hu, Huibin Huang

“…The expression of ALKBH5 in PTC cell lines BCPAP, IHH4, and TPC1 was significantly lower than Nthy-ori3-1 (P < 0.05). IHH4 and TPC1 cell lines were selected for subsequent experiments. …”
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甲状腺乳头状癌雌激素受体β表达及其 5′非翻译区甲基化 by 苟茜, 许琳婉, 刘智敏

“…【方法】应用 Western blot 和免疫组织化学染色法检测正常甲状腺细胞Nthy-ori3-1、PTC 细胞 BCPAP 以及正常甲状腺组织、甲状腺结节性增生组织和 PTC 组织ERβ蛋白表达；应用 qRT-PCR 检测各细胞和组织中 ERβ mRNA（0K-1）、ERβ mRNA（0N-1）和总 ERβ mRNA 表达水平；亚硫酸氢盐测序分析各细胞和组织中 ERβ 基因启动子 0K、启动子 0N 和外显子 0N 的 CpG 岛甲基化状态；DNA 甲基化转移酶抑制剂5-aza-2′-deoxycytidine（5-aza-dC）处理 Nthy-ori3-1 细胞和 BCPAP 细胞后，qRT-PCR 检测 ERβ mRNA（0K-1）、ERβ mRNA（0N-1）和总 ERβ mRNA 表达水平。…”
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Silencing NEAT1 suppresses thyroid carcinoma via miR-126/NEAT1/VEGFA axis by Weiwei Zeng, Yan Lin, Hai Lin, Xuemei Wu

“…We show that NEAT1 is highly expressed in Papillary Thyroid Carcinoma cell line (PTC-1) and anaplastic thyroid cancer cell line (SW1736) as compared with the human thyroid follicular epithelial cell line (Nthy-ori 3-1). shRNA knockdown of NEAT1 led to the inhibition of cell growth, invasion, migration and Epithelial to Mesenchymal Transition (EMT) of thyroid cancer cells. …”
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Anti-cancer effect of midazolam via downregulating YWHAH in papillary thyroid cancer cells by Yang Li, Ai-Ping Tan, Yu-Shan Zhong

“…Midazolam dose-dependently decreased the viability of thyroid cancer cells and demonstrated no cytotoxicity on Nthy-ori-3-1 cells. In addition, increasing concentrations of midazolam or silencing of YWHAH significantly inhibited thyroid cancer cell proliferation, migration and invasion and induced cell apoptosis. …”
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The Contribution of IgG Glycosylation to Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) and Complement-Dependent Cytotoxicity (CDC) in Hashimoto’s Thyroiditis: An in Vitro Mo... by Marta Ząbczyńska, Katarzyna Polak, Kamila Kozłowska, Grzegorz Sokołowski, Ewa Pocheć

“…The aim of the present study was to assess the impact of HT-specific IgG glycosylation in ADCC and CDC, using in vitro models. The normal thyroid Nthy-ori 3-1 cell line and thyroid carcinoma FTC-133 cells were used as the target cells. …”
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