Change in Lipofectamine Carrier as a Tool to Fine-Tune Immunostimulation of Nucleic Acid Nanoparticles by Hannah S. Newton, Yasmine Radwan, Jie Xu, Jeffrey D. Clogston, Marina A. Dobrovolskaia, Kirill A. Afonin

“…Nucleic acid nanoparticles (NANPs) require a carrier to allow for their intracellular delivery to immune cells. …”
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Anti-sporozoite antibodies and immunity to malaria in a rural Gambian population. by Marsh, K, Hayes, R, Carson, D, Otoo, L, Shenton, F, Byass, P, Zavala, F, Greenwood, B

“…A conserved repeated epitope, (NANP)3, of the circumsporozoite protein of Plasmodium falciparum has been identified previously as a putative target for artificially induced immunity to malaria. …”

Natural parasite exposure induces protective human anti-malarial antibodies. by Triller, G, Scally, S, Costa, G, Pissarev, M, Kreschel, C, Bosch, A, Marois, E, Sack, B, Murugan, R, Salman, A, Janse, C, Khan, S, Kappe, S, Adegnika, A, Mordmüller, B, Levashina, E, Julien, J, Wardemann, H

“…Antibodies against the NANP repeat of circumsporozoite protein (CSP), the major surface antigen of Plasmodium falciparum (Pf) sporozoites, can protect from malaria in animal models but protective humoral immunity is difficult to induce in humans. …”

Opportunities, Barriers, and a Strategy for Overcoming Translational Challenges to Therapeutic Nucleic Acid Nanotechnology by Afonin, Kirill A, Dobrovolskaia, Marina A, Church, George, Bathe, Mark

“…Significant progress in the development, production, and characterization of nucleic acid nanomaterials and nucleic acid nanoparticles (NANPs), as well as abundant proof-of-concept data, are paving the way toward biomedical applications of these materials. …”
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Opportunities, Barriers, and a Strategy for Overcoming Translational Challenges to Therapeutic Nucleic Acid Nanotechnology by Afonin, Kirill A, Dobrovolskaia, Marina A, Church, George, Bathe, Mark

“…Significant progress in the development, production, and characterization of nucleic acid nanomaterials and nucleic acid nanoparticles (NANPs), as well as abundant proof-of-concept data, are paving the way toward biomedical applications of these materials. …”
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High sporozoite antibody titers in conjunction with microscopically detectable blood infection display signatures of protection from clinical malaria by Offeddu, V, Olotu, A, Osier, F, Marsh, K, Matuschewski, K, Thathy, V

“…Although naturally acquired humoral responses against Pfspz and (NANP)5 were strongly correlated (p < 0.0001), 37% of Pfspz responders did not recognize (NANP)5. …”

High-density binding to Plasmodium falciparum circumsporozoite protein repeats by inhibitory antibody elicited in mouse with human immunoglobulin repertoire. by Iga Kucharska, Špela Binter, Rajagopal Murugan, Stephen W Scally, Julia Ludwig, Katherine Prieto, Elaine Thai, Giulia Costa, Kan Li, Gillian Q Horn, Yevel Flores-Garcia, Alexandre Bosch, Taylor Sicard, John L Rubinstein, Fidel Zavala, S Moses Dennison, Georgia D Tomaras, Elena A Levashina, Paul Kellam, Hedda Wardemann, Jean-Philippe Julien

“…Like other IGHV3-33/IGKV1-5-encoded anti-NANP antibodies, mAb 850 primarily utilizes its HCDR3 and germline-encoded aromatic residues to recognize its core NANP motif. …”
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Subclass and avidity of circumsporozoite protein specific antibodies associate with protection status against malaria infection by Kelly E. Seaton, Rachel L. Spreng, Milite Abraha, Matthew Reichartz, Michelle Rojas, Frederick Feely, Richard H. C. Huntwork, Sheetij Dutta, Sarah V. Mudrak, S. Munir Alam, Scott Gregory, Erik Jongert, Margherita Coccia, Fernando Ulloa-Montoya, Ulrike Wille-Reece, Georgia D. Tomaras, S. Moses Dennison

“…NANP6 IgG3 magnitude, CSP IgG1 magnitude, and total serum antibody dissociation phase area-under-the-curve for NANP6, CSP, NPNA3, and N-interface binding were also associated with protection status in the regimen adjusted univariate analysis. …”
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Safety, immunogenicity and efficacy of a pre-erythrocytic malaria candidate vaccine, ICC-1132 formulated in Seppic ISA 720. by Walther, M, Dunachie, S, Keating, S, Vuola, J, Berthoud, T, Schmidt, A, Maier, C, Andrews, L, Andersen, R, Gilbert, S, Poulton, I, Webster, D, Dubovsky, F, Tierney, E, Sarpotdar, P, Correa, S, Huntcooke, A, Butcher, G, Williams, J, Sinden, R, Thornton, G, Hill, A

“…This regimen induced anti-NANP antibodies in 10/11 and modest T cell responses. …”

Development of antimalarial human monoclonal antibodies against Plasmodium falciparum by Wang, LT

“…CSP has three domains: an N-terminus, a central region composed of repeating NPDP/NVDP/NANP tetrapeptides, and a C-terminus. A truncated form of CSP containing 19 NANP repeats and the C-terminus is the antigenic target of the world’s only approved malaria vaccine (RTS,S). …”

Investigating protective mechanisms of anti-sporozoite vaccines by Bellamy, DG

“…R21 is a virus-like particle-based (VLP-based) vaccine displaying the immunodominant NANP repeat region of the circumsporozoite protein (CSP) of <em>Plasmodium falciparum</em>. …”

Vaksin malaria dan perkembangannya by Perpustakaan UGM, i-lib

“…Later it was found that the sequence of NANP in the CS gene predominates with minor repeats of NVDP. …”

A multivalent Plasmodium falciparum circumsporozoite protein‐based nanoparticle malaria vaccine elicits a robust and durable antibody response against the junctional epitope and th... by Geetanjali Pendyala, J. Mauricio Calvo‐Calle, Alberto Moreno, Ravi S. Kane

“…We report the design of a SpyCatcher‐mi3‐nanoparticle‐based vaccine presenting multiple copies of a chimeric PfCSP (cPfCSP) antigen that incorporates these important “T1/junctional” epitopes as well as a reduced number of (NANP)n repeats. cPfCSP‐SpyCatcher‐mi3 was immunogenic in mice eliciting high and durable IgG antibody levels as well as a balanced antibody response against the T1/junctional region and the (NANP)n repeats. …”
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Epitope-coated polymer particles elicit neutralising antibodies against Plasmodium falciparum sporozoites by Benjamin J. Evert, Shuxiong Chen, Robyn McConville, Ryan W. J. Steel, Julie Healer, Justin A. Boddey, Lucas Huntimer, Bernd H. A. Rehm

“…Abstract The current Malaria RTS,S vaccine is based on virus-like particles (VLPs) comprising the NANP repetitive epitopes from the cicumsporozoite protein (CSP) of Plasmodium falciparum. …”
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Identification of a neutralizing epitope within minor repeat region of Plasmodium falciparum CS protein by J. Mauricio Calvo-Calle, Robert Mitchell, Rita Altszuler, Caroline Othoro, Elizabeth Nardin

“…In contrast, murine monoclonal antibodies specific for the major CS repeats (NANP)n could be isolated from peptide-immunized mice that had limited or no sporozoite-neutralizing activity. …”
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A VLP for validation of the Plasmodium falciparum circumsporozoite protein junctional epitope for vaccine development by Erwan Atcheson, Adrian V. S. Hill, Arturo Reyes-Sandoval

“…Peptides from PfCSP, particularly from outside the normal target of neutralizing antibodies, the central NANP repeat region, are screened for evidence of protective efficacy. …”
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Recenzja książki Wasyla G. Kremienia – prezydenta Narodowej Akademii Nauk Pedagogicznych Ukrainy – pt. "Filozofia antropocentryzmu w edukacyjnej przestrzeni", przetłumaczonej i opu... by Zygmunt Wiatrowski

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Combining antimalarial drugs and vaccine for malaria elimination campaigns: a randomized safety and immunogenicity trial of RTS,S/AS01 administered with dihydroartemisinin, piperaq... by Lorenz von Seidlein, Borimas Hanboonkunupakarn, Podjanee Jittamala, Pongphaya Pongsuwan, Kesinee Chotivanich, Joel Tarning, Richard M. Hoglund, Markus Winterberg, Mavuto Mukaka, Pimnara Peerawaranun, Pasathorn Sirithiranont, Zoe Doran, Christian F. Ockenhouse, Karen Ivinson, Cynthia Lee, Ashley J. Birkett, David C. Kaslow, Pratap Singhasivanon, Nicholas P.J. Day, Arjen M. Dondorp, Nicholas J. White, Sasithon Pukrittayakamee

“…One month after the last vaccination all study participants had seroconverted to the PfCSP (NANP)6, and the PfCSP Full Length (N + C-Terminal). …”
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Combining antimalarial drugs and vaccine for malaria elimination campaigns: a randomized safety and immunogenicity trial of RTS,S/AS01 administered with dihydroartemisinin, piperaq... by Von Seidlein, L, Hanboonkunupakarn, B, Jittamala, P, Pongsuwan, P, Chotivanich, K, Tarning, J, Hoglund, R, Winterberg, M, Mukaka, M, Peerawaranun, P, Sirithiranont, P, Doran, Z, Ockenhouse, C, Ivinson, K, Lee, C, Birkett, A, Kaslow, D, Singhasivanon, P, Day, N, Dondorp, A, White, N, Pukrittayakamee, S

“…One month after the last vaccination all study participants had seroconverted to the PfCSP (NANP)6, and the PfCSP Full Length (N + C-Terminal). …”

Protective effects of combining monoclonal antibodies and vaccines against the Plasmodium falciparum circumsporozoite protein. by Lawrence T Wang, Lais S Pereira, Patience K Kiyuka, Arne Schön, Neville K Kisalu, Rachel Vistein, Marlon Dillon, Brian G Bonilla, Alvaro Molina-Cruz, Carolina Barillas-Mury, Joshua Tan, Azza H Idris, Joseph R Francica, Robert A Seder

“…These C-CSP-specific mAbs had limited binding to sporozoites in vitro that was increased by combination with neutralizing human "repeat" mAbs against the NPDP/NVDP/NANP tetrapeptides in the central repeat region of PfCSP. …”
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