Efficient manipulation of gene expression using Natronobacterium gregoryi Argonaute in zebrafish by Zhangji Dong, Xu Chen, Run Zhuo, Yuanyuan Li, Zhihao Zhou, Ying Sun, Yan Liu, Mei Liu

“…Abstract Background Natronobacterium gregoryi Argonaute (NgAgo) was found to reduce mRNA without generating detectable DNA double-strand breaks in a couple of endogenous genes in zebrafish, suggesting its potential as a tool for gene knockdown. …”
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No evidence of genome editing activity from Natronobacterium gregoryi Argonaute (NgAgo) in human cells. by Parisa Javidi-Parsijani, Guoguang Niu, Meghan Davis, Pin Lu, Anthony Atala, Baisong Lu

“…A recent work reported that the argonaute protein from Natronobacterium gregoryi (NgAgo) had DNA-guided genome editing activity in mammalian cells. …”
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Prokaryotic Argonaute Protein from Natronobacterium gregoryi Requires RNAs To Activate for DNA Interference In Vivo by Jiani Xing, Lixia Ma, Xinzhen Cheng, Jinrong Ma, Ruyu Wang, Kun Xu, Joe S. Mymryk, Zhiying Zhang

“…Here, we investigate the mechanism by which pAgo from Natronobacterium gregoryi (NgAgo) targets plasmid and bacteriophage T7 DNA using a heterologous Escherichia coli-based model system. …”
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No evidence for genome editing in mouse zygotes and HEK293T human cell line using the DNA-guided Natronobacterium gregoryi Argonaute (NgAgo). by Nay Chi Khin, Jenna L Lowe, Lora M Jensen, Gaetan Burgio

“…A recently published research article reported that the extreme halophile archaebacterium Natronobacterium gregoryi Argonaute enzyme (NgAgo) could cleave the cellular DNA under physiological temperature conditions in cell line and be implemented as an alternative to CRISPR/Cas9 genome editing technology. …”
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Unexpected binding behaviors of bacterial Argonautes in human cells cast doubts on their use as targetable gene regulators. by Henriette O'Geen, Chonghua Ren, Nicole B Coggins, Sofie L Bates, David J Segal

“…Although Argonaute from Natronobacterium gregoryi (NgAgo) was recently shown unable to cleave genomic DNA in mammalian cells, the utility of NgAgo or other pAgos as a targetable DNA-binding platform for epigenetic editing has not been explored. …”
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Long-term monitoring of ultratrace nucleic acids using tetrahedral nanostructure-based NgAgo on wearable microneedles by Bin Yang, Haonan Wang, Jilie Kong, Xueen Fang

“…Here, we report a tetrahedral nanostructure-based Natronobacterium gregoryi Argonaute (NgAgo) for long-term stable monitoring of ultratrace unamplified nucleic acids (cell-free DNAs and RNAs) in vivo for sepsis on wearable device. …”
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Inhibition of KU70 and KU80 by CRISPR interference, not NgAgo interference, increases the efficiency of homologous recombination in pig fetal fibroblasts by Guo-ling LI, Rong QUAN, Hao-qiang WANG, Xiao-fang RUAN, Jian-xin MO, Cui-li ZHONG, Hua-qiang YANG, Zi-cong LI, Ting GU, De-wu LIU, Zhen-fang WU, Geng-yuan CAI, Xian-wei ZHANG

“…To improve the efficiency of HR, multiple CRISPR interference (CRISPRi) and Natronobacterium gregoryi Argonaute (NgAgo) interference (NgAgoi) systems have been designed for the knockdown of NHEJ key molecules, KU70, KU80, polynucleotide kinase/phosphatase (PNKP), DNA ligase IV (LIG4), and NHEJ1. …”
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