The <i>Verticillium dahliae</i> Small Cysteine-Rich Protein VdSCP23 Manipulates Host Immunity by Jie Wang, Dan Wang, Xiaobin Ji, Jun Wang, Steven J. Klosterman, Xiaofeng Dai, Jieyin Chen, Krishna V. Subbarao, Xiaojuan Hao, Dandan Zhang

“…In this study, we show that the small cysteine-rich protein VdSCP23 inhibits cell necrosis in <i>Nicotiana benthamiana</i> leaves, as well as the reactive oxygen species (ROS) burst, electrolyte leakage and the expression of defense-related genes. …”
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Functional Characterization of BrF3'H, Which Determines the Typical Flavonoid Profile of Purple Chinese Cabbage by Sangkyu Park, Hyo Lee, Myung Ki Min, Jihee Ha, Jaeeun Song, Chan Ju Lim, Jinpyo Oh, Saet Buyl Lee, Jong-Yeol Lee, Beom-Gi Kim

“…Transient expression of BrF3′H in Nicotiana benthamiana followed by infiltration of naringenin and DHK as substrates resulted in eriodictyol and quercetin production in the infiltrated leaves, demonstrating the functionality of BrF3′H in planta. …”
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Efficacy of a plant-produced clade 2.3.4.4 H5 influenza virus-like particle vaccine in layer hens by Celia Abolnik, Martha M. O'Kennedy, Mark-Anthony Murphy, Daniel B.R. Wandrag

“…Here, a recombinant virus-like particle (VLP) displaying the hemagglutinin protein of a clade 2.3.4.4b H5N8 HPAI strain was produced in tobacco plants (Nicotiana benthamiana) and its immunogenicity with four commercial adjuvants was compared in layer hens. …”
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Uso y Abuso de Substancias Psicoactivas en Colombia. XIII Exposición Temporal. by Hugo Sotomayor Tribín

“….&#13; &#13; El tabaco (Nicotiana spp) como estimulante y curativo, se fuma, se aspira en forma de rapé, se usa como enema -en algunas sociedades indígenas del Amazonas-, o se chupa, en una mezcla con sales de origen vegetal, en forma de ambil, en la Amazonia, de chimú, en la Orinoquia y de yua, en la Sierra Nevada de Santa Marta.&#13; &#13; El hayo o ayo o exs (coca en quechua, que a su vez viene del término aimará kkoka) (Erythroxylum coca y E. novogranatense var. trixillense) se mastica sólo o mezclado con cales de las piedras o de macerado de conchas, y a veces con ambil, chimú o yua para potenciar su efecto estimulante en las largas jornadas diurnas de trabajo y nocturnas de conversaciones o de pensamiento. …”
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Serological Cross-Reactions between Expressed VP2 Proteins from Different Bluetongue Virus Serotypes by Petra C. Fay, Fauziah Mohd Jaafar, Carrie Batten, Houssam Attoui, Keith Saunders, George P. Lomonossoff, Elizabeth Reid, Daniel Horton, Sushila Maan, David Haig, Janet M. Daly, Peter P. C. Mertens

“…Recombinant BTV VP2 proteins (rVP2) were expressed in <i>Nicotiana benthamiana</i>, based on sequence data for isolates of thirteen BTV serotypes (primarily from Europe), including three ‘novel’ serotypes (BTV-25, -26 and -27) and alternative topotypes of four serotypes. …”
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Genome-wide investigation of defensin genes in peanut (Arachis hypogaea L.) reveals AhDef2.2 conferring resistance to bacterial wilt by Kai Zhao, Rui Ren, Xingli Ma, Kunkun Zhao, Chengxin Qu, Di Cao, Qian Ma, Yingying Ma, Fangping Gong, Zhongfeng Li, Xingguo Zhang, Dongmei Yin

“…Overexpression of protein fusion AhDef2.2-YFP in Nicotiana benthamiana and peanut leaves increased resistance to R. solanacearum, suggesting its role in response to BW infection. …”
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The C4 Protein of TbLCYnV Promotes SnRK1 β2 Degradation Via the Autophagy Pathway to Enhance Viral Infection in <i>N. benthamiana</i> by Xinquan Li, Min Zhao, Wanyi Yang, Xueping Zhou, Yan Xie

“…In this study, we validated the interaction between the C4 protein encoded by tobacco leaf curl Yunnan virus (TbLCYnV) and the regulatory subunit β of <i>Nicotiana benthamiana</i> SnRK1, i.e., NbSnRK1 β2, and identified that the Asp22 of C4 is critical for TbLCYnV C4–NbSnRK1 β2 interactions. …”
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Conserved RXLR Effector Genes of Phytophthora infestans Expressed at the Early Stage of Potato Infection Are Suppressive to Host Defense by Junliang Yin, Junliang Yin, Biao Gu, Biao Gu, Guiyan Huang, Guiyan Huang, Yuee Tian, Junli Quan, Junli Quan, Hannele Lindqvist-Kreuze, Weixing Shan, Weixing Shan

“…Agrobacterium tumefaciens-mediated transient expression of the selected effector genes in Nicotiana benthamiana and potato demonstrated their potential virulence function, as shown by suppression of PAMP-triggered immunity (PTI) or/and effector-triggered immunity (ETI). …”
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Overexpression of BcERF3 increases the biosynthesis of saikosaponins in Bupleurum chinense by Wenjing Han, Jiao Xu, Hefang Wan, Lei Zhou, Bin Wu, Jianping Gao, Xinwei Guo, Chun Sui, Jianhe Wei

“…Transient expression of BcERF3 in the leaves of Nicotiana benthamiana resulted in intracellular localization of the protein in the nucleus. …”
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Identification of Chalcone Isomerase Family Genes and Roles of <i>CnCHI4</i> in Flavonoid Metabolism in <i>Camellia nitidissima</i> by Suhang Yu, Jiyuan Li, Ting Peng, Sui Ni, Yi Feng, Qiushi Wang, Minyan Wang, Xian Chu, Zhengqi Fan, Xinlei Li, Hengfu Yin, Wanchuan Ge, Weixin Liu

“…<i>CnCHI4</i> overexpression significantly increased flavonoid production in <i>Nicotiana tabacum</i> and <i>C. nitidissima</i>. The results of the dual-luciferase reporter assay and yeast one-hybrid system revealed that CnMYB7 was the key transcription factor that governed the transcription of <i>CnCHI4</i>. …”
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Identification of the Novel Effector RsIA_NP8 in Rhizoctonia solani AG1 IA That Induces Cell Death and Triggers Defense Responses in Non-Host Plants by Miaomiao Wei, Miaomiao Wei, Miaomiao Wei, Aijun Wang, Aijun Wang, Aijun Wang, Yao Liu, Yao Liu, Li Ma, Li Ma, Li Ma, Xianyu Niu, Xianyu Niu, Xianyu Niu, Aiping Zheng, Aiping Zheng, Aiping Zheng

“…We performed functional studies on effectors in R. solani AG1 IA and found that, of 11 putative effectors tested, only RsIA_NP8 caused necrosis in the leaves of Nicotiana benthamiana. The predicted signal peptide of this protein was required to induce cell death, whereas predicted N-glycosylation sites were not required. …”
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Phyllospheric Microbial Composition and Diversity of the Tobacco Leaves Infected by Didymella segeticola by Yu Huang, Yu Huang, Han-Cheng Wang, Liu-Ti Cai, Wenhong Li, Daiwei Pan, Ligang Xiang, Ligang Xiang, Xiankun Su, Zhong Li, Muhammad Faheem Adil, Imran Haider Shamsi

“…A Myriad of biotic and abiotic factors inevitably affects the growth and production of tobacco (Nicotiana tabacum L.), which is a model crop and sought-after worldwide for its foliage. …”
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Panax notoginseng WRKY Transcription Factor 9 Is a Positive Regulator in Responding to Root Rot Pathogen Fusarium solani by Lilei Zheng, Lilei Zheng, Bingling Qiu, Bingling Qiu, Linlin Su, Linlin Su, Hanlin Wang, Hanlin Wang, Xiuming Cui, Xiuming Cui, Feng Ge, Feng Ge, Diqiu Liu, Diqiu Liu

“…The overexpression of PnWRKY9 in tobacco (Nicotiana tabacum) considerably increased the resistance to F. solani, whereas an RNAi-mediated decrease in the PnWRKY9 expression level in P. notoginseng leaves increased the susceptibility to F. solani. …”
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Glucose Supply Induces PsMYB2-Mediated Anthocyanin Accumulation in Paeonia suffruticosa ‘Tai Yang’ Cut Flower by Lili Zhang, Li Yan, Li Yan, Chao Zhang, Xin Kong, Yiqing Zheng, Li Dong

“…Ectopic overexpression of PsMYB2 in Nicotiana tabacum resulted in a strong pigmentation in petals and stamens of tobacco flowers accompanied with multiple anthocyanin biosynthetic genes upregulated. …”
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Cloning and functional analysis of the FAD2 gene family from desert shrub Artemisia sphaerocephala by Xiumei Miao, Lijing Zhang, Xiaowei Hu, Shuzhen Nan, Xiaolong Chen, Hua Fu

“…Transient expression of AsFAD2 genes in the Nicotiana benthamiana leaves revealed that the encoded proteins were all located in the endoplasmic reticulum. …”
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Analyses of MiRNA Functions in Maize Using a Newly Developed ZMBJ-CMV-2bN81-STTM Vector by Xuedong Liu, Sijia Liu, Rong Wang, Xi Chen, Zaifeng Fan, Boming Wu, Tao Zhou

“…Our results showed that ZMBJ-CMV-2bN81-STTM can be used to investigate miRNA function in Nicotiana benthamiana and maize seedlings. The ZMBJ-CMV-2bN81-STTM-based downregulation of Nbe-miR165/166 or Nbe-miR159 induced specific and strong miRNA-sequestering phenotypes, and increased the expressions of their predicted target genes. …”
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Synergism Among the Four Tobacco Bushy Top Disease Casual Agents in Symptom Induction and Aphid Transmission by Xiaojiao Chen, Hengming Luo, Jingyi Zhang, Yan Ma, Kehua Li, Feng Xiong, Yahui Yang, Jiazhen Yang, Pingxiu Lan, Taiyun Wei, Yi Xu, Hairu Chen, Fan Li

“…In addition, inoculation of tobacco K326 plants with TBTV alone caused necrosis in systemic leaves by 7 dpi. Tobacco K326 and Nicotiana benthamiana plants infected by single virus or multiple viruses showed very different disease symptoms at various dpi. …”
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Mutations in PpAGO3 Lead to Enhanced Virulence of Phytophthora parasitica by Activation of 25–26 nt sRNA-Associated Effector Genes by Junjie Xu, Yilin Li, Jinbu Jia, Wenjing Xiong, Chengcheng Zhong, Guiyan Huang, Xiuhong Gou, Yuling Meng, Weixing Shan

“…These mutants showed increased pathogenicity on both Nicotiana benthamiana and Arabidopsis thaliana plants. …”
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Nuclear Localization of HopA1_Pss61 Is Required for Effector-Triggered Immunity by Hobin Kang, Quang-Minh Nguyen, Arya Bagus Boedi Iswanto, Jong Chan Hong, Saikat Bhattacharjee, Walter Gassmann, Sang Hee Kim

“…Interestingly, nuclear localization of <i>HopA1_Pss61</i> is required to induce cell death as NES-<i>HopA1_Pss61</i> suppresses the level of cell death in <i>Nicotiana benthamiana</i>. In addition, in planta expression of <i>hopA1_Pss61</i> led to defense responses, such as a dwarf morphology, a cell death response, inhibition of bacterial growth, and increased accumulation of defense marker proteins in transgenic Arabidopsis. …”
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Fusarium graminearum Effector FgNls1 Targets Plant Nuclei to Induce Wheat Head Blight by Guixia Hao, Todd A. Naumann, Hui Chen, Guihua Bai, Susan McCormick, Hye-Seon Kim, Bin Tian, Harold N. Trick, Michael J. Naldrett, Robert Proctor

“…A fusion protein of green fluorescent protein and FgNls1 accumulated in plant cell nuclei when transiently expressed in Nicotiana benthamiana. FgNls1 suppressed Bax-induced cell death when co-expressed in N. benthamiana. …”
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