Development of antifungal biopharmaceutical dectin1-FC fusion by Goh, Melody

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Altering Tetrapyrrole Biosynthesis by Overexpressing <i>Ferrochelatases</i> (<i>Fc1</i> and <i>Fc2</i>) Improves Photosynthetic Efficiency in Transgenic Barley by Dilrukshi S. K. Nagahatenna, Jingwen Tiong, Everard J. Edwards, Peter Langridge, Ryan Whitford

“…In photosynthetic organisms studied so far, there is evidence for two FC isoforms, which are encoded by two genes (<i>FC1</i> and <i>FC2</i>). Previous studies suggest that these two genes are required for the production of two physiologically distinct heme pools with only <i>FC2</i>-derived heme involved in photosynthesis. …”
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Immunoglobulin G1 Fc glycosylation as an early hallmark of severe COVID-19 by Tamas Pongracz, Jan Nouta, Wenjun Wang, Krista E. van Meijgaarden, Federica Linty, Gestur Vidarsson, Simone A. Joosten, Tom H.M. Ottenhoff, Cornelis H. Hokke, Jutte J.C. de Vries, Sesmu M. Arbous, Anna H.E. Roukens, Manfred Wuhrer

“…Methods: In this prospective, observational single center cohort study, IgG1 Fc glycosylation was analyzed by liquid chromatography-mass spectrometry following affinity capturing from serial plasma samples of 159 SARS-CoV-2 infected hospitalized patients. …”
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Agonist redirected checkpoint, PD1-Fc-OX40L, for cancer immunotherapy by George Fromm, Suresh de Silva, Kellsey Johannes, Arpita Patel, Josiah C. Hornblower, Taylor H. Schreiber

“…Importantly, all agonist functions of PD1-Fc-OX40L are independent of Fc receptor cross-linking. …”
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Lipopolysaccharide Impedes Bone Repair in <i>FcγRIIB</i>-Deficient Mice by Sirikanda Jantaboon, Nithidol Sakunrangsit, Parichart Toejing, Asada Leelahavanichkul, Prapaporn Pisitkun, Matthew B. Greenblatt, Sutada Lotinun

“…An osteoblast-associated gene (<i>Col1a1</i>) was attenuated in <i>FcγRIIB</i>-deficient mice, whereas <i>Bglap, Hhip,</i> and <i>Creb5</i> were further downregulated with LPS treatment in <i>FcγRIIB^−/−</i> mice compared to <i>FcγRIIB^−/−</i> mice. …”
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CX3CL1-Fc treatment prevents atherosclerosis in Ldlr KO mice by Matthew Riopel, Melanie Vassallo, Erik Ehinger, Jennifer Pattison, Karen Bowden, Holger Winkels, Maria Wilson, Ron de Jong, Sanjay Patel, Deepika Balakrishna, James Bilakovics, Andrea Fanjul, Artur Plonowski, Christopher J. Larson, Klaus Ley, Pedro Cabrales, Joseph L. Witztum, Jerrold M. Olefsky, Yun Sok Lee

“…Moreover, CX3CL1-Fc administration reduced diet-induced atherosclerosis after switching from an atherogenic to a normal chow diet. …”
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The Impact of Immunoglobulin G1 Fc Sialylation on Backbone Amide H/D Exchange by Felix Kuhne, Lea Bonnington, Sebastian Malik, Marco Thomann, Cecile Avenal, Florian Cymer, Harald Wegele, Dietmar Reusch, Michael Mormann, Patrick Bulau

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Improving the Pharmacodynamics and In Vivo Activity of ENPP1‐Fc Through Protein and Glycosylation Engineering by Paul R. Stabach, Kristin Zimmerman, Aaron Adame, Dillon Kavanagh, Christopher T. Saeui, Christian Agatemor, Shawn Gray, Wenxiang Cao, Enrique M. De La Cruz, Kevin J. Yarema, Demetrios T. Braddock

“…First, we added new N‐glycans to ENPP1; second, we optimized pH‐dependent cellular recycling by protein engineering of the Fc neonatal receptor; finally, we used a two‐step process to improve sialylation by first producing ENPP1‐Fc in cells stably transfected with human α‐2,6‐sialyltransferase (ST6) and further enhanced terminal sialylation by supplementing production with 1,3,4‐O‐Bu3ManNAc. …”
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Profiling the Biophysical Developability Properties of Common IgG1 Fc Effector Silencing Variants by Robert Pejchal, Anthony B. Cooper, Michael E. Brown, Maximiliano Vásquez, Eric M. Krauland

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Functional humanization of immunoglobulin heavy constant gamma 1 Fc domain human FCGRT transgenic mice by Benjamin E. Low, Gregory J. Christianson, Emily Lowell, Wenning Qin, Michael V. Wiles

“…Here, we used CRISPR/Cas9-mediated homology-directed repair to equip the hFCGRT Tg32 strain with a human IGHG1 Fc domain. This replacement now results in mice that produce human IgG1 Fc-mouse IgG Fab2 chimeric antibodies at physiologically relevant levels, which can be further heightened by immunization. …”
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ENPP1-Fc prevents neointima formation in generalized arterial calcification of infancy through the generation of AMP by Yvonne Nitschke, Yan Yan, Insa Buers, Kristina Kintziger, Kim Askew, Frank Rutsch

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Crystal structure of the HSV-1 Fc receptor bound to Fc reveals a mechanism for antibody bipolar bridging. by Elizabeth R Sprague, Chu Wang, David Baker, Pamela J Bjorkman

“…The ternary organization of the gE-gI/Fc complex is compatible with antibody bipolar bridging, which can interfere with the antiviral immune response.…”
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Therapeutic benefits of recombinant alpha1-antitrypsin IgG1 Fc-fusion protein in experimental emphysema by Katsuyuki Takeda, Soo-Hyun Kim, Anthony Joetham, Irina Petrache, Erwin W. Gelfand

“…Using mouse models of emphysema, we compared the effects of a recombinant AAT-IgG1 Fc-fusion protein (AAT-Fc), which is expected to have a longer half-life following infusion, to those of pAAT. …”
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Long-Term Hypermethylation of <i>FcγR2B</i> in Leukocytes of Patients with Kawasaki Disease by Ling-Sai Chang, Hong-Ren Yu, Chiao-Lun Chu, Kuang-Den Chen, Ying-Hsien Huang, Mindy Ming-Huey Guo, Ken-Pen Weng, Ho-Chang Kuo

Subjects: “…<i>FcγR2B</i> methylation…”
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Recombinant pregnancy-specific glycoprotein-1-Fc reduces functional deficit in a mouse model of permanent brain ischaemia by Kyle Malone, Jennifer A. Shearer, John M. Williams, Anne C. Moore, Tom Moore, Christian Waeber

“…This establishes proof-of-concept of rPSG1-Fc as a potential stroke immunotherapy.…”
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Suppression of allograft rejection by Tim-1-Fc through cross-linking with a novel Tim-1 binding partner on T cells. by Liang Xiao, Zhi-ren Fu, Fang Liu, Lu-ding Zhang, Xiao-min Shi, Xiao-yun Shen, Zhi-jia Ni, Hong Fu, Rui-dong Li, Xue-tao Cao, Guo-shan Ding, Quan-xing Wang

“…Here we investigated the role of Tim-1 in T-cell responses and allograft rejection using recombinant human Tim-1 extracellular domain and IgG1-Fc fusion proteins (Tim-1-Fc). In vitro assays confirmed that Tim-1-Fc selectively binds to CD4(+) effector T cells, but not dendritic cells or natural regulatory T cells (nTregs). …”
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Biophysical differences in IgG1 Fc-based therapeutics relate to their cellular handling, interaction with FcRn and plasma half-life by Torleif Tollefsrud Gjølberg, Rahel Frick, Simone Mester, Stian Foss, Algirdas Grevys, Lene Støkken Høydahl, Øystein Kalsnes Jørstad, Tilman Schlothauer, Inger Sandlie, Morten C. Moe, Jan Terje Andersen

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Engineered IgG1-Fc Molecules Define Valency Control of Cell Surface Fcγ Receptor Inhibition and Activation in Endosomes by Elizabeth M. Bailey, Elizabeth M. Bailey, Amit Choudhury, Harika Vuppula, Harika Vuppula, Daniel F. Ortiz, John Schaeck, Anthony M. Manning, Carlos J. Bosques, Adam D. Hoppe, Adam D. Hoppe

“…Previously, we demonstrated that an engineered tri-valent arrangement of IgG1 Fc domains (SIF1) potently inhibited FcγR activation by IC, whereas a penta-valent Fc molecule (PentX) activated FcγR, potentially mimicking ICs and leading to Syk phosphorylation. …”
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FcMgv1, FcStuA AND FcVeA BASED GENETIC CHARACTERIZATION IN Fusarium culmorum (W.G. Smith) by Emre Yörük, Özlem Sefer

“…In this study, in vitro growth capacity of 33 F. culmorum isolates originating from Turkey and the relationship between phenotypic and genetic characteristics obtained based on similarities of FcMgv1, FcStuA and FcVeA genes were investigated. Linear growth rate values were recorded at 4th and 7th days of incubation. …”
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Natural killer cells are crucial for the efficacy of Icon (factor VII/human IgG1 Fc) immunotherapy in human tongue cancer by Hu Zhiwei, Li Jing

“…</p> <p>Results</p> <p>We showed that Icon, as a chimeric factor VII and human IgG1 Fc immunoconjugate, could separately induce murine natural killer (NK) cells and activate complement to kill TCA8113 cancer cells <it>in vitro </it>via antibody dependent cell-mediated cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). …”
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