Limitations of rapid diagnostic tests in malaria surveys in areas with varied transmission intensity in Uganda 2017-2019: Implications for selection and use of HRP2 RDTs. by Agaba B Bosco, Joaniter I Nankabirwa, Adoke Yeka, Sam Nsobya, Karryn Gresty, Karen Anderson, Paul Mbaka, Christiane Prosser, David Smith, Jimmy Opigo, Rhoda Namubiru, Emmanuel Arinaitwe, John Kissa, Samuel Gonahasa, Sungho Won, Bora Lee, Chae Seung Lim, Charles Karamagi, Qin Cheng, Joan K Nakayaga, Moses R Kamya

“…<h4>Background</h4>Plasmodium falciparum histidine-rich protein 2 (HRP2)-based rapid diagnostic tests (RDTs) are exclusively recommended for malaria diagnosis in Uganda; however, their functionality can be affected by parasite-related factors that have not been investigated in field settings.…”
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Acute Uncomplicated Febrile Illness in Children Aged 2-59 months in Zanzibar - Aetiologies, Antibiotic Treatment and Outcome. by Kristina Elfving, Deler Shakely, Maria Andersson, Kimberly Baltzell, Abdullah S Ali, Marc Bachelard, Kerstin I Falk, Annika Ljung, Mwinyi I Msellem, Rahila S Omar, Philippe Parola, Weiping Xu, Max Petzold, Birger Trollfors, Anders Björkman, Magnus Lindh, Andreas Mårtensson

“…Two patients were positive for Plasmodium falciparum. Respiratory syncytial virus (24.5%), influenza A/B (22.3%), rhinovirus (10.5%) and group-A streptococci (6.4%), CXR-confirmed pneumonia (6.2%), Shigella (4.3%) were the most common viral and bacterial fever diagnoses, respectively. …”
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Profiling of myristoylation in Toxoplasma gondii reveals an N-myristoylated protein important for host cell penetration by Malgorzata Broncel, Caia Dominicus, Luis Vigetti, Stephanie D Nofal, Edward J Bartlett, Bastien Touquet, Alex Hunt, Bethan A Wallbank, Stefania Federico, Stephen Matthews, Joanna C Young, Edward W Tate, Isabelle Tardieux, Moritz Treeck

“…Here we describe the myristoylated proteome of Toxoplasma gondii using chemoproteomic methods and show that a small-molecule NMT inhibitor developed against related Plasmodium spp. is also functional in Toxoplasma. We identify myristoylation on a transmembrane protein, the microneme protein 7 (MIC7), which enters the secretory pathway in an unconventional fashion with the myristoylated N-terminus facing the lumen of the micronemes. …”
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Malaria Parasite Stress Tolerance Is Regulated by DNMT2-Mediated tRNA Cytosine Methylation by Elie Hammam, Ameya Sinha, Sebastian Baumgarten, Flore Nardella, Jiaqi Liang, Samia Miled, Frédéric Bonhomme, Diane Erdmann, Benoit Arcangioli, Paola B. Arimondo, Peter Dedon, Peter Preiser, Artur Scherf

“…The molecular mechanisms that protect Plasmodium falciparum homeostasis during the complex life cycle remain unknown. …”
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Validation of a Mobile Health Technology Platform (FeverTracker) for Malaria Surveillance in India: Development and Usability Study by Ipsita Pal Bhowmick, Dibyajyoti Chutia, Avinash Chouhan, Nilay Nishant, P L N Raju, Kanwar Narain, Harpreet Kaur, Rocky Pebam, Jayanta Debnath, Rabindra Tripura, Kongkona Gogoi, Suman Ch Nag, Aatreyee Nath, Debabrata Tripathy, Jotish Debbarma, Nirapada Das, Ujjwal Sarkar, Rislyn Debbarma, Rajashree Roy, Bishal Debnath, Dipanjan Dasgupta, Suraj Debbarma, Kamal Joy Tripura, Guneram Reang, Amit Sharma, Manju Rahi, Jyoti Chhibber-Goel

“…The use of the app in 19 tribal villages of the Dhalai district in Tripura, India, assisted in the surveillance of 1880 suspected malaria patients and confirmed malaria infection in 93.4% (114/122; Plasmodium falciparum), 4.9% (6/122; P vivax), and 1.6% (2/122; P falciparum/P vivax mixed infection) of cases. …”
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Preclinical immunogenicity and safety of the cGMP-grade placental malaria vaccine PRIMVACResearch in context by Arnaud Chêne, Stéphane Gangnard, Anna Guadall, Hervé Ginisty, Odile Leroy, Nicolas Havelange, Nicola K. Viebig, Benoît Gamain

“…Keywords: Malaria, Plasmodium, Vaccine, VAR2CSA, Placenta…”
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Molecular surveillance of anti-malarial drug resistance in Democratic Republic of Congo: high variability of chloroquinoresistance and lack of amodiaquinoresistance by Doudou M. Yobi, Nadine K. Kayiba, Dieudonné M. Mvumbi, Raphael Boreux, Pius Z. Kabututu, Hippolyte N. T. Situakibanza, Joris L. Likwela, Patrick De Mol, Emile W. Okitolonda, Niko Speybroeck, Georges L. Mvumbi, Marie-Pierre Hayette

“…The purpose of this study was to determine the prevalence of K76T mutations in circulating strains of Plasmodium falciparum, 16 years after CQ withdrawal in the DRC and to investigate the presence of the SVMNT haplotype. …”
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Indoor residual spraying with micro-encapsulated pirimiphos-methyl (Actellic® 300CS) against malaria vectors in the Lake Victoria basin, Tanzania. by Fabian M Mashauri, Alphaxard Manjurano, Safari Kinung'hi, Jackline Martine, Eric Lyimo, Coleman Kishamawe, Chacha Ndege, Mahdi M Ramsan, Adeline Chan, Charles D Mwalimu, John Changalucha, Stephen Magesa

“…Ofthe 34 An. funestus group indentified 91.2% (n = 31) were An. parensis and 8.8% (n = 3) were An. rivulorum. The overall Plasmodium falciparum sporozoite rate was 0.7% (n = 2,098). …”
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Neuregulin-1 attenuates experimental cerebral malaria (ECM) pathogenesis by regulating ErbB4/AKT/STAT3 signaling by Mingli Liu, Wesley Solomon, Juan Carlos Cespedes, Nana O. Wilson, Byron Ford, Jonathan K. Stiles

“…Utilizing an experimental cerebral malaria (ECM) model (Plasmodium berghei ANKA in C57BL/6), we reported that NRG-1 played a cytoprotective role in ECM and that circulating levels were inversely correlated with ECM severity. …”
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Safe and effective delivery of supplemental iron to healthy older adults: The double-blind, randomized, placebo-controlled trial protocol of the Safe Iron Study [version 2; peer re... by Erin D. Lewis, Dayong Wu, Joel B. Mason, Athar H. Chishti, John M. Leong, Kathryn Barger, Simin N. Meydani, Gerald F. Combs

“…The primary outcomes of this study include ex vivo malaria (Plasmodium falciparum) infectivity of host erythrocytes, ex vivo bacterial proliferation (of selected species) in presence of host plasma and intestinal inflammation assessed by fecal calprotectin. …”
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Deep Immune Phenotyping and Single-Cell Transcriptomics Allow Identification of Circulating TRM-Like Cells Which Correlate With Liver-Stage Immunity and Vaccine-Induced Protection... by Andrés Noé, Mehreen S. Datoo, Amy Flaxman, Mohammad Ali Husainy, Daniel Jenkin, Duncan Bellamy, Rebecca A. Makinson, Richard Morter, Fernando Ramos Lopez, Jonathan Sheridan, Dimitrios Voukantsis, Naveen Prasad, Adrian V. S. Hill, Katie J. Ewer, Alexandra J. Spencer

“…Protection from liver-stage malaria requires high numbers of CD8+ T cells to find and kill Plasmodium-infected cells. A new malaria vaccine strategy, prime-target vaccination, involves sequential viral-vectored vaccination by intramuscular and intravenous routes to target cellular immunity to the liver. …”
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Changes in Serological Immunology Measures in UK and Kenyan Adults Post-Controlled Human Malaria Infection by Susanne Helena Hodgson, David Llewellyn, Sarah Silk, Kathryn H Milne, Sean C Elias, Kazutoyo Miura, Gathoni Kamuyu, Elizabeth Juma, Charles Magiri, Alfred Musau Muia, Jing Jin, Alexandra J Spencer, Rhea Jessica Longley, Thomas Mercier, Laurent Decosterd, Carole A Long, Faith HA Osier, Stephen Hoffman, Bernhards Ragama Ogutu, Adrian V.S. Hill, Kevin Marsh, Simon J Draper

“…This study used aseptic, cryopreserved, attenuated Plasmodium falciparum sporozoites administered by needle and syringe and was the first to evaluate parasite dynamics post-CHMI in individuals with varying degrees of prior exposure to malaria. …”
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Mortality risk among children admitted in a large-scale nutritional program in Niger, 2006. by Nael Lapidus, Andrea Minetti, Ali Djibo, Philippe J Guerin, Sarah Hustache, Valérie Gaboulaud, Rebecca F Grais

“…Additional data included results of a malaria rapid diagnostic test due to Plasmodium falciparum (Paracheck) and whether the child was a resident of the region of Maradi or came from bordering Nigeria to seek treatment. …”
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Plasma cytokine profiles associated with rhodesiense sleeping sickness and falciparum malaria co-infection in North Eastern Uganda by Julius Nsubuga, Charles Drago Kato, Ann Nanteza, Enock Matovu, Vincent Pius Alibu

“…This study determined prevalence of Plasmodium falciparum malaria among Trypanosoma brucei rhodesiense HAT and plasma cytokine profile levels associated with HAT and/or malaria infections. …”
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Population Pharmacokinetic Properties of Piperaquine in Falciparum Malaria: An Individual Participant Data Meta-Analysis. by Richard M Hoglund, Lesley Workman, Michael D Edstein, Nguyen Xuan Thanh, Nguyen Ngoc Quang, Issaka Zongo, Jean Bosco Ouedraogo, Steffen Borrmann, Leah Mwai, Christian Nsanzabana, Ric N Price, Prabin Dahal, Nancy C Sambol, Sunil Parikh, Francois Nosten, Elizabeth A Ashley, Aung Pyae Phyo, Khin Maung Lwin, Rose McGready, Nicholas P J Day, Philippe J Guerin, Nicholas J White, Karen I Barnes, Joel Tarning

“…<h4>Background</h4>Artemisinin-based combination therapies (ACTs) are the mainstay of the current treatment of uncomplicated Plasmodium falciparum malaria, but ACT resistance is spreading across Southeast Asia. …”
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Early malaria infection, dysregulation of angiogenesis, metabolism and inflammation across pregnancy, and risk of preterm birth in Malawi: A cohort study. by Robyn E Elphinstone, Andrea M Weckman, Chloe R McDonald, Vanessa Tran, Kathleen Zhong, Mwayiwawo Madanitsa, Linda Kalilani-Phiri, Carole Khairallah, Steve M Taylor, Steven R Meshnick, Victor Mwapasa, Feiko O Ter Kuile, Andrea L Conroy, Kevin C Kain

“…Malaria prevalence was high; 70% (n = 1,138) had PCR-positive Plasmodium falciparum infection at least once over the course of pregnancy and/or positive placental histology. …”
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<i>A</i> New Benzopyranyl Cadenane Sesquiterpene and Other Antiplasmodial and Cytotoxic Metabolites from <i>Cleistochlamys kirkii</i> by Stephen S. Nyandoro, Gasper Maeda, Joan J.E. Munissi, Amra Gruhonjic, Paul A. Fitzpatrick, Sofia Lindblad, Sandra Duffy, Jerry Pelletier, Fangfang Pan, Rakesh Puttreddy, Vicky M. Avery, Máté Erdélyi

“…The ethanolic crude extract of <i>C. kirkii</i> root bark gave 72% inhibition against the chloroquine-sensitive 3D7-strain malaria parasite <i>Plasmodium falciparum</i> at 0.01 &#956;g/mL. The isolated metabolites dichamanetin, (<i>E</i>)-acetylmelodorinol, and cleistenolide showed IC₅₀ = 9.3, 7.6 and 15.2 &#956;M, respectively, against <i>P. falciparum</i> 3D7. …”
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Preclinical Trial of Traditional Plant Remedies for the Treatment of Complications of Gestational Malaria by Peter Uchenna Amadi, Emmanuel Nnabugwu Agomuo, Chinyere Nneka Ukaga, Uche Chinedu Njoku, Joy Adaku Amadi, Chinweuba Godswill Nwaekpe

“…<b>Aim:</b> This study examined the therapeutic efficacies of AI, PS, or a combination of AI and PS in a pregnant rat model for exoerythrocytic stages of <i>Plasmodium falciparum</i> parasite. <b>Method:</b> A predetermined sample size of 30 dams was used (for a power level and confidence interval of 95%), and divided equally into six groups made up of non-malarous dams, untreated malarous dams, and malarous dams either treated exclusively with 1 mL of 3000 mg/kg b.w AI, 1000 mg/kg b.w PS, AI + PS (50% <i>v</i>/<i>v</i>), or 25 mg/kg b.w CQ. …”
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Nouveaux triterpènes à activité antiplasmodiale isolés des feuilles de Neoboutonia macrocalyx L., une plante consommée par les chimpanzés du parc national de Kibale (Ouganda) by Jane Namukobe, Bernard T Kiremire, Robert Byamukama, John M Kasenene, Vincent Dumontet, Françoise Guéritte, Sabrina Krief, Isabelle Florent, John D Kabasa

“…Les composés isolés ont été évalués pour leur activité antiplasmodiale sur la souche chloroquino-résistante de Plasmodium falciparum FcB1 ainsi que pour leur cytotoxicité sur la souche de cellules KB (carcinome de l’épiderme nasopharyngien humain) et sur la souche de cellules MRC5 (fibroblastes embryonnaires humains). …”
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Analysis of the potential for a malaria vaccine to reduce gaps in malaria intervention coverage by H. Juliette T. Unwin, Lazaro Mwandigha, Peter Winskill, Azra C. Ghani, Alexandra B. Hogan

“…Methods Using data from household surveys, the overlap between malaria intervention coverage and childhood vaccination (diphtheria-tetanus-pertussis dose 3, DTP3) uptake in 20 African countries with at least one first administrative level unit with Plasmodium falciparum parasite prevalence greater than 10% was calculated. …”
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