Systems-Based Analysis of the <italic toggle="yes">Sarcocystis neurona</italic> Genome Identifies Pathways That Contribute to a Heteroxenous Life Cycle by Tomasz Blazejewski, Nirvana Nursimulu, Viviana Pszenny, Sriveny Dangoudoubiyam, Sivaranjani Namasivayam, Melissa A. Chiasson, Kyle Chessman, Michelle Tonkin, Lakshmipuram S. Swapna, Stacy S. Hung, Joshua Bridgers, Stacy M. Ricklefs, Martin J. Boulanger, Jitender P. Dubey, Stephen F. Porcella, Jessica C. Kissinger, Daniel K. Howe, Michael E. Grigg, John Parkinson

“…IMPORTANCE Sarcocystis neurona is a member of the coccidia, a clade of single-celled apicomplexan parasites responsible for major economic and health care burdens worldwide. A cousin of Plasmodium, Cryptosporidium, Theileria, and Eimeria, Sarcocystis is one of the most successful parasite genera; it is capable of infecting all vertebrates (fish, reptiles, birds, and mammals—including humans). …”
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Antiparasitic Meroterpenoids Isolated from <i>Memnoniella dichroa</i> CF-080171 by Frederick Boye Annang, Guiomar Pérez-Moreno, Cristina Bosch-Navarrete, Victor González-Menéndez, Jesús Martín, Thomas A. Mackenzie, Maria C. Ramos, Luis M. Ruiz-Pérez, Olga Genilloud, Dolores González-Pacanowska, Francisca Vicente, Fernando Reyes

“…A <i>Memnoniella dichroa</i> CF-080171 extract was identified to exhibit potent activity against <i>Plasmodium falciparum</i> 3D7 and <i>Trypanosoma cruzi</i> Tulahuen whole parasites in a high-throughput screening (HTS) campaign of microbial extracts from the Fundación MEDINA’s collection. …”
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Molecular Drivers of Multiple and Elevated Resistance to Insecticides in a Population of the Malaria Vector <i>Anopheles gambiae</i> in Agriculture Hotspot of West Cameroon by Arnaud Tepa, Jonas A. Kengne-Ouafo, Valdi S. Djova, Magellan Tchouakui, Leon M. J. Mugenzi, Rousseau Djouaka, Constant A. Pieme, Charles S. Wondji

“…Characterization of the breeding water and soil from Mangoum was achieved using the HPLC technique; (3) Results: <i>An. gambiae</i> s.s. was the only species in Mangoum with 4.10% infection with <i>Plasmodium.</i> These mosquitoes were resistant to all the four classes of insecticides with mortality rates <7% for pyrethroids and DDT and <54% for carbamates and organophophates. …”
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TNFR1 signaling converging on FGF14 controls neuronal hyperactivity and sickness behavior in experimental cerebral malaria by Nolan M. Dvorak, Nadia D. Domingo, Cynthia M. Tapia, Paul A. Wadsworth, Mate Marosi, Yosef Avchalumov, Chanida Fongsaran, Leandra Koff, Jessica Di Re, Catherine M. Sampson, Timothy J. Baumgartner, Pingyuan Wang, Paula P. Villarreal, Olivia D. Solomon, Sonja J. Stutz, Aditi, Jacob Porter, Komi Gbedande, Brendan Prideaux, Thomas A. Green, Erin H. Seeley, Parimal Samir, Kelley T. Dineley, Gracie Vargas, Jia Zhou, Irma Cisneros, Robin Stephens, Fernanda Laezza

“…On account of the dependency of hyperinflammatory experimental cerebral malaria (eCM) on TNF, we performed patch-clamp studies in slices from eCM mice and showed that Plasmodium chabaudi infection augments Nav1.6 channel conductance of CA1 pyramidal neurons through the TNFR1–JAK2–FGF14–Nav1.6 signaling network, which leads to hyperexcitability. …”
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Semi-Synthesis of <i>N</i>-Aryl Amide Analogs of Piperine from <i>Piper nigrum</i> and Evaluation of Their Antitrypanosomal, Antimalarial, and Anti-SARS-CoV-2 Main Protease Activit... by Rattanaporn Wansri, Aye Chan Khine Lin, Jutharat Pengon, Sumalee Kamchonwongpaisan, Nitipol Srimongkolpithak, Roonglawan Rattanajak, Patcharin Wilasluck, Peerapon Deetanya, Kittikhun Wangkanont, Kowit Hengphasatporn, Yasuteru Shigeta, Jatupol Liangsakul, Aphinya Suroengrit, Siwaporn Boonyasuppayakorn, Taksina Chuanasa, Wanchai De-eknamkul, Supot Hannongbua, Thanyada Rungrotmongkol, Supakarn Chamni

“…Its half-maximal inhibitory concentration (IC₅₀) for antitrypanosomal activity against <i>Trypanosoma brucei rhodesiense</i> was 15.46 ± 3.09 μM, and its antimalarial activity against the 3D7 strain of <i>Plasmodium falciparum</i> was 24.55 ± 1.91 μM, which were fourfold and fivefold more potent, respectively, than the activities of piperine. …”
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DIAGNÓSTICO CITOMORFOLÓGICO DE CAPNOCYTOPHAGA CANIMORSUS EM INFECÇÕES HUMANAS by CAC Franchini, PC Daher, IY Takihi, CF Matias, LAM Oliveira, MV Gonçalves, MCA Silva, ADSB Perazzio, MLLF Chauffaille, AF Sandes

“…Os microrganismos mais comumente encontrados no sangue periférico incluem Plasmodium spp., Trypanosoma cruzi, Babesia spp., Leishmania spp. e Histoplasma capsulatum. …”
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RELATO DE CASO: LOMBALGIA DECORRENTE DE CRISE FALCÊMICA by GE Dresch, BR Campos, MA Souza, DL Queiroz, MAF Chaves, MF Barros

“…O maior número de afetados do mundo encontra-se localizado na população africana por conta do traço genético da doença, que por sua vez protege os indivíduos da infecção Plasmodium (malária) o que é muito presente no país. …”
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Indoor and outdoor malaria transmission in two ecological settings in rural Mali: implications for vector control by Moussa Keïta, Sidy Doumbia, Ibrahim Sissoko, Mahamoudou Touré, Sory Ibrahim Diawara, Drissa Konaté, Ambièlè Bernard Sodio, Sekou F. Traoré, Mahamadou Diakité, Seydou O. Doumbia, Nafomon Sogoba, Donald J. Krogstad, Jeffrey G. Shaffer, Mamadou B. Coulibaly

“…Enzyme-Linked -Immuno-Sorbent Assay (ELISA) were used to determine the origin of mosquito blood meals and presence of Plasmodium falciparum sporozoite infections. Results A total of 11,237 An. gambiae sensu lato (s.l.) were collected during the study period (5239 and 5998 from the Dangassa and Koïla Bamanan sites, respectively). …”
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The Multistage Antimalarial Compound Calxinin Perturbates <i>P. falciparum</i> Ca²⁺ Homeostasis by Targeting a Unique Ion Channel by Yash Gupta, Neha Sharma, Snigdha Singh, Jesus G. Romero, Vinoth Rajendran, Reagan M. Mogire, Mohammad Kashif, Jordan Beach, Walter Jeske, Poonam, Bernhards R. Ogutu, Stefan M. Kanzok, Hoseah M. Akala, Jennifer Legac, Philip J. Rosenthal, David J. Rademacher, Ravi Durvasula, Agam P. Singh, Brijesh Rathi, Prakasha Kempaiah

“…Calxinin exhibits potent inhibitory activity in the nanomolar range against the asexual blood stages of drug-sensitive (3D7), multidrug-resistant (Dd2), artemisinin-resistant (IPC4912), and fresh Kenyan field isolated <i>Plasmodium falciparum</i> strains. Calxinin treatment resulted in diminished maturation of parasite sexual precursor cells (gametocytes) accompanied by distorted parasite morphology. …”
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Targeted amplicon deep sequencing of ama1 and mdr1 to track within-host P. falciparum diversity throughout treatment in a clinical drug trial [version 4; peer review: 2 approved, 2... by Mainga Hamaluba, Juliana Wambua, Arjen M. Dondorp, Jennifer Musyoki, Caroline Ngetsa, Oksana Kharabora, Leonard Ndwiga, Zaydah de Laurent, Kevin Wamae, Kelvin Kimenyi, Gabriel Mwambingu, Peter Kalume, Philip Bejon, Jeffrey Bailey, Rob van der Pluijm, Lynette Ochola-Oyier, Jonathan Juliano, Victor Osoti

“…In this study, AmpSeq of Plasmodium falciparum apical membrane antigen 1 (ama1), and multidrug resistance gene 1 (mdr1), were used to characterise the complexity of infection (COI) and drug-resistance genotypes, respectively. …”
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17β-Estradiol Is Involved in the Sexual Dimorphism of the Immune Response to Malaria by Luis Antonio Cervantes-Candelas, Jesús Aguilar-Castro, Fidel Orlando Buendía-González, Omar Fernández-Rivera, Teresita de Jesús Nolasco-Pérez, Monserrat Sofía López-Padilla, David Roberto Chavira-Ramírez, Martha Legorreta-Herrera

“…In this study, we decreased in vivo the levels of 17β-estradiol by gonadectomy or administered 17β-estradiol to intact or gonadectomized male and female CBA/Ca mice infected with Plasmodium berghei ANKA. Subsequently, we assessed the effects of 17β-estradiol on parasite load; the percentages of different immune cells in the spleen; the plasma levels of antibodies and pro- and anti-inflammatory cytokines; and the mRNA expression levels of cytokine-encoding genes in the brain. …”
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Intermittent screening and treatment with artemisinin-combination therapy versus intermittent preventive treatment with sulphadoxine-pyrimethamine for malaria in pregnancy: a syste... by Julie R Gutman, Carole Khairallah, Kasia Stepniewska, Harry Tagbor, Mwayiwawo Madanitsa, Matthew Cairns, Anne Joan L'lanziva, Linda Kalilani, Kephas Otieno, Victor Mwapasa, Steve Meshnick, Simon Kariuki, Daniel Chandramohan, Meghna Desai, Steve M. Taylor, Brian Greenwood, Feiko O. ter Kuile

“…Generalised linear regression was used to compare adverse pregnancy outcomes (composite of small-for-gestational-age, low birthweight (LBW), or preterm delivery) and peripheral or placental Plasmodium falciparum at delivery. The effects of subpatent (PCR-positive, RDT/microscopy-negative) infections were assessed in both arms pooled using multi-variable fixed-effect models adjusting for the number of patent infections. …”
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Hemoglobinopathies, merozoite surface protein-2 gene polymorphisms, and acquisition of Epstein Barr virus among infants in Western Kenya by Perez K. Olewe, Shehu Shagari Awandu, Elly O. Munde, Samuel B. Anyona, Evans Raballah, Asito S. Amolo, Sidney Ogola, Erick Ndenga, Clinton O. Onyango, Rosemary Rochford, Douglas J. Perkins, Collins Ouma

“…Abstract Background Epstein Barr virus (EBV)-associated endemic Burkitt’s Lymphoma pediatric cancer is associated with morbidity and mortality among children resident in holoendemic Plasmodium falciparum regions in western Kenya. P. falciparum exerts strong selection pressure on sickle cell trait (SCT), alpha thalassemia (-α3.7/αα), glucose-6-phosphate dehydrogenase (G6PD), and merozoite surface protein 2 (MSP-2) variants (FC27, 3D7) that confer reduced malarial disease severity. …”
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Targeted amplicon deep sequencing of ama1 and mdr1 to track within-host P. falciparum diversity throughout treatment in a clinical drug trial [version 2; peer review: 2 approved, 1... by Mainga Hamaluba, Juliana Wambua, Arjen M. Dondorp, Jennifer Musyoki, Caroline Ngetsa, Oksana Kharabora, Leonard Ndwiga, Zaydah de Laurent, Kevin Wamae, Kelvin Kimenyi, Gabriel Mwambingu, Peter Kalume, Philip Bejon, Jeffrey Bailey, Rob van der Pluijm, Lynette Ochola-Oyier, Jonathan Juliano, Victor Osoti

“…In this study, AmpSeq of Plasmodium falciparum apical membrane antigen 1 (ama1), and multidrug resistance gene 1 (mdr1), were used to characterise the complexity of infection (COI) and drug-resistance genotypes, respectively. …”
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Composition of the gut microbiota transcends genetic determinants of malaria infection severity and influences pregnancy outcomeResearch in context by Catherine D. Morffy Smith, Minghao Gong, Alicer K. Andrew, Brittany N. Russ, Yong Ge, Mojgan Zadeh, Caitlin A. Cooper, Mansour Mohamadzadeh, Julie M. Moore

“…Interpretation: The composition of the gut microbiota in Plasmodium chabaudi chabaudi AS-infected pregnant Swiss Webster mice transcends the outbred genetics of the Swiss Webster mouse stock as a determinant of malaria infection severity, subsequently influencing pregnancy outcomes in malaria-exposed progeny. …”
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Targeted amplicon deep sequencing of ama1 and mdr1 to track within-host P. falciparum diversity throughout treatment in a clinical drug trial [version 3; peer review: 2 approved, 2... by Mainga Hamaluba, Juliana Wambua, Arjen M. Dondorp, Jennifer Musyoki, Caroline Ngetsa, Oksana Kharabora, Leonard Ndwiga, Zaydah de Laurent, Kevin Wamae, Kelvin Kimenyi, Gabriel Mwambingu, Peter Kalume, Philip Bejon, Jeffrey Bailey, Rob van der Pluijm, Lynette Ochola-Oyier, Jonathan Juliano, Victor Osoti

“…In this study, AmpSeq of Plasmodium falciparum apical membrane antigen 1 (ama1), and multidrug resistance gene 1 (mdr1), were used to characterise the complexity of infection (COI) and drug-resistance genotypes, respectively. …”
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Comparative metabolism and tolerability of racemic primaquine and its enantiomers in human volunteers during 7-day administration by Washim Khan, Yan-Hong Wang, Narayan D. Chaurasiya, N. P. Dhammika Nanayakkara, H. M. Bandara Herath, Kerri A. Harrison, Gray Dale, Donald A. Stanford, Eric P. Dahl, James D. McChesney, Waseem Gul, Mahmoud A. ElSohly, Mahmoud A. ElSohly, Mahmoud A. ElSohly, David Jollow, Babu L. Tekwani, Larry A. Walker

“…Primaquine (PQ) is an 8-aminoquinoline antimalarial, active against dormant Plasmodium vivax hypnozoites and P. falciparum mature gametocytes. …”
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Understanding and maximising the community impact of seasonal malaria chemoprevention in Burkina Faso (INDIE-SMC): study protocol for a cluster randomised evaluation trial by John Bradley, Alphonse Ouedraogo, Issiaka Soulama, Chris Drakeley, Teun Bousema, Alfred B Tiono, Denise Hien, Sodiomon Bienvenu Sirima, Issa N Ouedraogo, Jean Baptist Yaro, Aïssata Barry, Seyi Soremekun, Marta Moreno, Jean Sawadogo, Markus Gmeiner, Samuel S Sermé, Isabel Byrne, Jordache Ramjith, Lynn Grignard, Simon Koele, Rob ter Heine, Amidou Z Ouedraogo, Edith Sanogo

“…While highly impactful in reducing Plasmodium falciparum malaria burden in controlled research settings, the impact of SMC on infection prevalence is moderate in real-life settings. …”
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Structure-guided insights into potential function of novel genetic variants in the malaria vaccine candidate PfRh5 by Khadidiatou Mangou, Adam J. Moore, Laty Gaye Thiam, Aboubacar Ba, Alessandra Orfanó, Ife Desamours, Duncan Ndungu Ndegwa, Justin Goodwin, Yicheng Guo, Zizhang Sheng, Saurabh D. Patel, Fatoumata Diallo, Seynabou D. Sene, Mariama N. Pouye, Awa Thioub Faye, Alassane Thiam, Vanessa Nunez, Cheikh Tidiane Diagne, Bacary Djilocalisse Sadio, Lawrence Shapiro, Ousmane Faye, Alassane Mbengue, Amy K. Bei

“…A major challenge to developing an efficacious vaccine is the extensive diversity of Plasmodium falciparum antigens. While genetic diversity plays a major role in immune evasion and is a barrier to the development of both natural and vaccine-induced protective immunity, it has been under-prioritized in the evaluation of malaria vaccine candidates. …”
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Iron Promotes Dihydroartemisinin Cytotoxicity via ROS Production and Blockade of Autophagic Flux via Lysosomal Damage in Osteosarcoma by Ying Shen, Ying Shen, Ying Shen, Bin Zhang, Bin Zhang, Bin Zhang, Yanwei Su, Yanwei Su, Yanwei Su, Shaikh Atik Badshah, Shaikh Atik Badshah, Xiaofei Wang, Xin Li, Xin Li, Xin Li, Yanru Xue, Yanru Xue, Yanru Xue, Li Xie, Li Xie, Zhe Wang, Zhe Wang, Zhouqi Yang, Zhouqi Yang, Ge Zhang, Peng Shang, Peng Shang

“…Dihydroartemisinin (DHA), a classic anti-malarial drug, kills plasmodium through iron-dependent ROS generation. In this research, we observed the anti-osteosarcoma effects and mechanisms of DHA. …”
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