Site-specific integration and expression of an anti-malarial gene in transgenic Anopheles gambiae significantly reduces Plasmodium infections. by Janet M Meredith, Sanjay Basu, Derric D Nimmo, Isabelle Larget-Thiery, Emma L Warr, Ann Underhill, Clare C McArthur, Victoria Carter, Hilary Hurd, Catherine Bourgouin, Paul Eggleston

“…Expression of Vida3, specifically in the midgut of bloodfed females, offered consistent and significant protection against Plasmodium yoelii nigeriensis, reducing average parasite intensity by 85%. …”
Get full text

Apoptosis of non-parasitized red blood cells in malaria: a putative mechanism involved in the pathogenesis of anaemia by Daniel-Ribeiro Cláudio T, Banic Dalma M, Silva Luciene A, Magalhães Aline D, Totino Paulo RR, Ferreira-da-Cruz Maria

“…</p> <p>Results</p> <p>As expected, experimental infection of Balb/c mice with <it>Plasmodium yoelii </it>17XL parasites was characterized by progressive increase of parasitaemia and acute anaemia, leading to death. …”
Get full text

A rapid and scalable density gradient purification method for <it>Plasmodium</it> sporozoites by Kennedy Mark, Fishbaugher Matthew E, Vaughan Ashley M, Patrapuvich Rapatbhorn, Boonhok Rachasak, Yimamnuaychok Narathatai, Rezakhani Nastaran, Metzger Peter, Ponpuak Marisa, Sattabongkot Jetsumon, Kappe Stefan H, Hume Jen CC, Lindner Scott E

“…Moreover, it can be effectively applied to both human (<it>Plasmodium falciparum</it>, <it>Plasmodium vivax</it>) and rodent (<it>Plasmodium yoelii</it>) infective species with excellent recovery rates.…”
Get full text

Effects of autacoid inhibitors and of an antagonist on malaria infection in mice by E.O. Iwalewa, E.O. Agbani

“…The effects of p-chlorophenylalanine, an inhibitor of serotonin synthesis, indomethacin, an inhibitor of prostaglandin synthesis, cyproheptadine, a serotonin, bradykinin and histamine antagonist, were assessed separately and in combination with chloroquine (CQ) in Vom strains of Swiss albino mice (18-22 g) of either sex infected intraperitoneally with 1 x 10(7) Plasmodium yoelii nigeriensis-induced malaria. As prophylactic, these agents reduced from 31.9 ± 4.5 to 16.1 ± 8.1% the level of parasitemia relative to control but had no appreciable activity as curative agents when administered subcutaneously once daily for 4 days after 72 h of parasites innoculum in vivo. …”
Get full text

Alpha-tocopherol transfer protein disruption confers resistance to malarial infection in mice by Takeya Motohiro, Yokoyama Naoaki, Fukumoto Shinya, Shichiri Mototada, Ishibashi Kana, Chiba Mayumi, Ichikawa Chie, Ueta Yoshiko Y, Herbas Maria S, Xuan Xuenan, Arai Hiroyuki, Suzuki Hiroshi

“…</p> <p>Methods</p> <p>α-TTP knockout mice were infected with <it>Plasmodium berghei </it>NK65 or <it>Plasmodium yoelii </it>XL-17, parasitaemia, survival rate were monitored. …”
Get full text

Human T cell recognition of the blood stage antigen Plasmodium hypoxanthine guanine xanthine phosphoribosyl transferase (HGXPRT) in acute malaria. by Woodberry, T, Pinzon-Charry, A, Piera, K, Panpisutchai, Y, Engwerda, C, Doolan, D, Salwati, E, Kenangalem, E, Tjitra, E, Price, R, Good, M, Anstey, N

“… BACKGROUND: The Plasmodium purine salvage enzyme, hypoxanthine guanine xanthine phosphoribosyl transferase (HGXPRT) can protect mice against Plasmodium yoelii pRBC challenge in a T cell-dependent manner and has, therefore, been proposed as a novel vaccine candidate. …”

The requirement for potent adjuvants to enhance the immunogenicity and protective efficacy of protein vaccines can be overcome by prior immunization with a recombinant adenovirus. by de Cassan, S, Forbes, E, Douglas, A, Milicic, A, Singh, B, Gupta, P, Chauhan, V, Chitnis, C, Gilbert, S, Hill, A, Draper, S

“…This human adenovirus serotype 5-protein regimen also induced a more cytophilic Ab response and demonstrated improved efficacy of merozoite surface protein-1 protein vaccines against a Plasmodium yoelii blood-stage challenge. This indicates that the differential immunogenicity of protein vaccine adjuvants may be largely overcome by prior immunization with recombinant adenovirus, especially for adjuvants that are traditionally considered poorly immunogenic in the context of subunit vaccination and may circumvent the need for more potent chemical adjuvants.…”

Memory CD8 T cell responses exceeding a large but definable threshold provide long-term immunity to malaria. by Schmidt, N, Podyminogin, R, Butler, N, Badovinac, V, Tucker, B, Bahjat, K, Lauer, P, Reyes-Sandoval, A, Hutchings, C, Moore, A, Gilbert, S, Hill, A, Bartholomay, L, Harty, J

“…Infection of mice with sporozoites of Plasmodium berghei or Plasmodium yoelii has been used extensively to evaluate liver-stage protection by candidate preerythrocytic malaria vaccines. …”

Evaluation of approaches to identify the targets of cellular immunity on a proteome-wide scale. by Fernanda C Cardoso, Joanne S Roddick, Penny Groves, Denise L Doolan

“…In vitro and in vivo studies in mice immunized with the Plasmodium yoelii circumsporozoite DNA vaccine with or without IVTT protein boost showed antigen-specific cytokine production using purified IVTT antigens only. …”
Get full text

Analysis of innate defences against <it>Plasmodium falciparum </it>in immunodeficient mice by Van Rooijen Nico, Mejia Pedro, Tyagi Rajeev, Arnold Ludovic, Pérignon Jean-Louis, Druilhe Pierre

“…The innate responses towards the murine parasite <it>Plasmodium yoelii </it>were used as a control.</p> <p>Results</p> <p>Results show that 1) <it>P. falciparum </it>induces a strong inflammation characterized by an increase in circulating leukocytes and the release of inflammatory cytokines; 2) in contrast, the rodent parasite <it>P. yoelii</it>, induces a far more moderate inflammation; 3) human red blood cells and the anti-inflammatory agents employed induce low-grade inflammation; and 4) macrophages seem to bear the most critical function in controlling <it>P. falciparum </it>survival in those mice, whereas polymorphonuclear and NK cells have only a minor role.…”
Get full text

Transstadial immune activation in a mosquito: Adults that emerge from infected larvae have stronger antibacterial activity in their hemocoel yet increased susceptibility to malaria... by Lisa D. Brown, Lillian L. M. Shapiro, Grayson A. Thompson, Tania Y. Estévez‐Lao, Julián F. Hillyer

“…A larval infection, however, does not have a meaningful effect on the probability that female adults will survive a systemic bacterial infection, and increases the susceptibility of females to Plasmodium yoelii, as measured by oocyst prevalence and intensity in the midgut. …”
Get full text

Co-expression of Interleukin-15 Enhances the Protective Immune Responses Induced by Immunization with a Murine Malaria MVA-Based Vaccine Encoding the Circumsporozoite Protein. by Marcela Parra, Xia Liu, Steven C Derrick, Amy Yang, Alvaro Molina-Cruz, Carolina Barillas-Mury, Hong Zheng, Phuong Thao Pham, Martha Sedegah, Arnel Belmonte, Dianne D Litilit, Thomas A Waldmann, Sanjai Kumar, Sheldon L Morris, Liyanage P Perera

“…Here we describe the development and characterization of a novel modified vaccinia virus Ankara (MVA)-based malaria vaccine which co-expresses the Plasmodium yoelii circumsporozoite protein (CSP) and IL-15. …”
Get full text

A structural annotation resource for the selection of putative target proteins in the malaria parasite by Joubert Fourie, Joubert Yolandi

“…</p> <p>Methods</p> <p>An integrated structural annotation system was developed and applied to <it>P. falciparum</it>, <it>Plasmodium vivax </it>and <it>Plasmodium yoelii</it>. The annotation included searches for sequence similarity, patterns and domains in addition to the following predictions: secondary structure, transmembrane helices, protein disorder, low complexity, coiled-coils and small molecule interactions. …”
Get full text

Human T cell recognition of the blood stage antigen <it>Plasmodium </it>hypoxanthine guanine xanthine phosphoribosyl transferase (HGXPRT) in acute malaria by Woodberry Tonia, Pinzon-Charry Alberto, Piera Kim A, Panpisutchai Yawalak, Engwerda Christian R, Doolan Denise L, Salwati Ervi, Kenangalem Enny, Tjitra Emiliana, Price Ric N, Good Michael F, Anstey Nicholas M

“…<p>Abstract</p> <p>Background</p> <p>The <it>Plasmodium </it>purine salvage enzyme, hypoxanthine guanine xanthine phosphoribosyl transferase (HGXPRT) can protect mice against <it>Plasmodium yoelii </it>pRBC challenge in a T cell-dependent manner and has, therefore, been proposed as a novel vaccine candidate. …”
Get full text

MalVac: Database of malarial vaccine candidates by Singh Harinder, Ansari Faraz, Ahmed Shakil, Chaudhuri Rupanjali, Ramachandran Srinivasan

“…These predicted vaccine candidates are the adhesins and adhesin-like proteins from Plasmodium species, <it>Plasmodium falciparum</it>, <it>Plasmodium vivax </it>and <it>Plasmodium yoelii</it>. Subsequently, these protein sequences were analysed through 20 publicly available algorithms to obtain Orthologs, Paralogs, BetaWraps, TargetP, TMHMM, SignalP, CDDSearch, BLAST with Human Ref. …”
Get full text

An Attenuated HSV-1-Derived Malaria Vaccine Expressing Liver-Stage Exported Proteins Induces Sterilizing Protection against Infectious Sporozoite Challenge by Paul J. F. Rider, Mohd Kamil, Ilknur Yilmaz, Habibe N. Atmaca, Merve Kalkan-Yazici, Mehmet Ziya Doymaz, Konstantin G. Kousoulas, Ahmed S. I. Aly

“…Intramuscular and subcutaneous immunizations of mice with a pooled vaccine, composed of the three attenuated virus strains expressing each LS antigen, induced sterile protection against the intravenous challenge of <i>Plasmodium yoelii</i> 17X-NL salivary gland sporozoites. …”
Get full text

Genome comparison of human and non-human malaria parasites reveals species subset-specific genes potentially linked to human disease. by Christian Frech, Nansheng Chen

“…We combined sequence similarity searches with synteny block analyses to identify species subset-specific genes in chromosome-internal regions of six published Plasmodium genomes, including Plasmodium falciparum, Plasmodium vivax, Plasmodium knowlesi, Plasmodium yoelii, Plasmodium berghei, and Plasmodium chabaudi. …”
Get full text

Molecular analysis of non-specific protection against murine malaria induced by BCG vaccination. by Marcela Parra, Xia Liu, Steven C Derrick, Amy Yang, Jinhua Tian, Kristopher Kolibab, Sanjai Kumar, Sheldon L Morris

“…To further evaluate whether BCG immunization protects against malarial parasitemia and to define molecular correlates of this non-specific immunity, mice were vaccinated with BCG and then challenged 2 months later with asexual blood stage Plasmodium yoelii 17XNL (PyNL) parasites. Following challenge with PyNL, significant decreases in parasitemia were observed in BCG vaccinated mice relative to naïve controls. …”
Get full text

Suppression of Host p53 Is Critical for Plasmodium Liver-Stage Infection by Alexis Kaushansky, Albert S. Ye, Laura S. Austin, Sebastian A. Mikolajczak, Ashley M. Vaughan, Nelly Camargo, Peter G. Metzger, Alyse N. Douglass, Gavin MacBeath, Stefan H.I. Kappe

“…Using protein lysate microarrays, we found that Plasmodium yoelii rodent malaria parasites perturb hepatocyte regulatory pathways involved in cell survival, proliferation, and autophagy. …”
Get full text

Comparison of male reproductive success in malaria-refractory and susceptible strains of <it>Anopheles gambiae</it> by Voordouw Maarten J, Koella Jacob C, Hurd Hilary

“…</p> <p>Methods</p> <p>Aspects of the male cost of carrying <it>Plasmodium</it>-refractory genes were estimated by comparing the males' immune response and reproductive success among strains of <it>Anopheles gambiae </it>that had been selected for refractoriness or extreme susceptibility to the rodent malaria parasite, <it>Plasmodium yoelii nigeriensis</it>. The refractory males had a stronger melanization response than males from the susceptible line. …”
Get full text