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Autochthonous visceral leishmaniasis in the Russian Federation and the role of polymerase chain reaction as an alternative diagnostic method
Published 2022-11-01“…On the territory of the Russian Federation there are foci of autochthonous visceral leishmaniasis in the Crimea and the North Caucasus. Polymerase chain reaction is an alternative to bone marrow aspirate microscopy for the diagnosis of visceral leishmaniasis.…”
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Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection
Published 2017-02-01Subjects: “…convective polymerase chain reaction…”
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POSSIBILITY OF USING NESTED POLYMERASE CHAIN REACTION FOR DIAGNOSTICS OF DISEASES CAUSED BY VARICELLA ZOSTER VIRUS
Published 2015-06-01“…Monocyte fraction of venous blood cells, pretreated with heparin, was isolated by centrifugation in ficoll-verografin density gradient, total DNA was then isolated from cells by phenol-chloroform extraction with subsequent precipitation with alcohol. Polymerase chain reaction was carried out in thermocyclers Tercyc and TProfessional Gradient (Biometra), amplified DNA was analyzed by electrophoresis on 1.6% agarose gel in the presence of ethidium bromide. …”
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Assessment of Cost-Efficient Thermocycler Prototype for Polymerase Chain Reaction and Loop-Mediated Isothermal Amplification
Published 2021-12-01Subjects: “…dna amplification-based diagnostic, thermocycler prototype, polymerase chain reaction (pcr), and loop-mediated isothermal amplification (lamp)…”
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227
Sex Identification of the Sun Conure (Aratinga solstitialis) Using Calamus Based on Polymerase Chain Reaction
Published 2024-01-01“…Therefore, Determination between male and female carried out by their morphology examination. The Polymerase Chain Reaction (PCR) method, utilizing molecular-based technology, was employed to determine the sex of Aratinga solstitialis in this study. …”
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Kit for diagnosis of BK virus infection by the real-time Polymerase Chain Reaction method (PCR)
Published 2018-02-01“…The presented work comprises information about developed by Republic Scientific and Practical Center for Epidemiology and Microbiology the diagnostic kit for detection the BK virus DNA in clinical samples by the real-time Polymerase Chain Reaction method. Accomplished studies showed high level its diagnostic sensitivity and specificity. …”
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Fabrication of Polymerase Chain Reaction Plastic Lab-on-a-Chip Device for Rapid Molecular Diagnoses
Published 2016-05-01Subjects: Get full text
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False negative result of polymerase chain reaction in very early stages of acute retinal necrosis
Published 2023-11-01Subjects: Get full text
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Polymerase chain reaction-based detection of coinfecting DNA viruses in Vietnamese pigs in 2017 and 2021
Published 2022-10-01“…Materials and Methods: A total of 174 porcine circovirus 2-positive samples from pigs (n = 86 for 2017 and n = 88 for 2021), including from the sera and internal organs, across 11 provinces were examined by polymerase chain reaction. Results: This study demonstrated the wide distribution of DNA viruses among pig farms in Vietnam in 2021, with the detection rate for PPV ranging from 3.4% to 27.3% among PPV1-PPV4. …”
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Open Thermal Control System for Stable Polymerase Chain Reaction on a Digital Microfluidic Chip
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Molecular identification of Giardia duodenalis in Ecuador by polymerase chain reaction-restriction fragment length polymorphism
Published 2013-06-01“…All Giardia positive samples (based on an ELISA assay) were analysed using a semi-nested polymerase chain reaction-restriction fragment length polymorphism assay that targets the glutamate dehydrogenase (gdh) gene; those amplified were subsequently genotyped using NlaIV and RsaI enzymes. …”
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Detection of respiratory viruses by real-time polymerase chain reaction in outpatients with acute respiratory infection
Published 2014-09-01“…Twenty-three pathogenic respiratory agents, 18 respiratory viruses and five bacteria were investigated using multiplex real-time reverse transcriptase polymerase chain reaction (RT-PCR) and indirect immunofluorescence assay (IIF). …”
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Development of ISO Standard for Real-Time Polymerase Chain Reaction Detection of Turkey-Derived Material
Published 2024-01-01Subjects: “…turkey; real-time polymerase chain reaction; detection; international organization for standardization…”
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Detection of 5 kinds of common foodborne pathogens by GeXP multiplex polymerase chain reaction
Published 2020-07-01Subjects: “…gexp multiplex polymerase chain reaction…”
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Polymerase chain reaction with two molecular targets in mucosal leishmaniasis' diagnosis: a validation study
Published 2007-08-01Subjects: Get full text
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Simplified Method for Preservation and Polymerase Chain Reaction-amplification of Trypanosoma cruzi DNA in Human Blood
Published 1997-03-01Subjects: Get full text
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Molecular bird sexing of sulphur‐crested cockatoo (Cacatua galerita) by polymerase chain reaction method
Published 2021-03-01“…The purpose of this study was to determine the sex of Sulphur‐crested cockatoo (Cacatua galerita) by detecting differences in the intron size of the chromodomain helicase DNA‐binding 1 (CHD1) gene on the Z and W chromosomes by polymerase chain reaction (PCR) method and to compare of plucked feathers and blood samples as DNA sources for molecular bird sexing. …”
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Low-Cost, Real-Time Polymerase Chain Reaction System for Point-of-Care Medical Diagnosis
Published 2022-03-01Subjects: “…polymerase chain reaction (PCR)…”
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