Evaluation of reverse transcription-polymerase chain reaction and simultaneous amplification and testing for quantitative detection of serum hepatitis B virus RNA by Xiaohan Hu, Liwei Zhao, Mingrong Ou, Yuxin Chen, Hongxia Wei, Yanyan Xia, Hongpan Xu, Miao Li, Jun Wang

Subjects: “…Reverse transcription quantitative polymerase chain reaction…”
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Identifikasi Carmovirus pada Tanaman Anyelir melalui Teknik Reverse Transcription-Polymerase Chain Reaction dan Analisis Sikuen Nukleotida by Rizki Haerunisa, Erniawati Diningsih, Gede Suastika

“…This study aimed to identify the virus causing leaf mottling on carnation using reverse transcription-polymerase chain reaction (RT-PCR) method and nucleotide sequence analysis. …”
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Plasmid-based controls to detect rpoB mutations in Mycobacterium tuberculosis by quantitative polymerase chain reaction-high-resolution melting by Joas Lucas da Silva, Gabriela Guimaraes Sousa Leite, Gisele Medeiros Bastos, Beatriz Cacciacarro Lucas, Daniel Keniti Shinohara, Joice Sayuri Takinami, Marcelo Miyata, Cristina Moreno Fajardo, André Ducati Luchessi, Clarice Queico Fujimura Leite, Rosilene Fressatti Cardoso, Rosario Dominguez Crespo Hirata, Mario Hiroyuki Hirata

“…Quantitative polymerase chain reaction-high-resolution melting (qPCR-HRM) analysis was used to screen for mutations related to drug resistance in Mycobacterium tuberculosis. …”
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Prevalence Determination of m. Hominis and m. Genitalium in the Semen Samples in the Northeast of Iran Using Culture and Multiplex Polymerase Chain Reaction by Kh Moridi, K Ghazvini, M Hemmati, H Hoseiniun, M Torkaman, M. H Fallah Mehrabadi

“…The presence of M. hominis and M. genitalium was detected by cultivation, polymerase chain reaction (PCR), and Multiplex PCR assays. …”
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Seasonal Stability Assessment of Reference Genes for Quantitative Real-Time Polymerase Chain Reaction Normalization in <i>Bombus terrestris</i> by Kathannan Sankar, Kyeong-Yong Lee, Kyu-Won Kwak, Su-Jin Lee, Young-Bo Lee

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Investigation on the applicability of a long-range reverse-transcription quantitative polymerase chain reaction assay for the rapid detection of active viruses by Masato Yasuura, Yuki Nakaya, Hiroki Ashiba, Takashi Fukuda

“…Abstract Background Although conventional polymerase chain reaction (PCR) methods are widely used in diagnosis, the titer of the pathogenic virus is difficult to determine based on the PCR. …”
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Chip-based digital Polymerase Chain Reaction as quantitative technique for the detection of PIK3CA mutations in breast cancer patients by Stefano Giannoni-Luza, Oscar Acosta, Alexis Germán Murillo Carrasco, Pierina Danos, José Manuel Cotrina Concha, Henry Guerra Miller, Joseph A. Pinto, Alfredo Aguilar, Jhajaira M. Araujo, Ricardo Fujita, Jose Buleje

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Detection of Mycobacterium leprae DNA by polymerase chain reaction in the blood of individuals, eight years after completion of anti-leprosy therapy by Adalberto Rezende Santos, Vivian Balassiano, Maria Leide W Oliveira, Marcia Aparecida da Silva Pereira, Patricia Barros Santos, Wim Maurits Degrave, Philip Noel Suffys

“…Thirty eight patients with indeterminate leprosy (HI), at least 4 to 6 years after discharge from multibacillary (MB) or paucibacillary (PB) schemes of anti leprosy multidrug therapy (MDT), were submitted to traditional diagnostic procedures for leprosy and to polymerase chain reaction (PCR) analysis of different clinical samples for detection of Mycobacterium leprae DNA. …”
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Detection of Respiratory Syncytial Virus and Human Metapneumovirus in Children with Respiratory Tract Infection by Use Real Time Polymerase Chain Reaction by Hala Luay Abduljabbar, Areej Atiyah Hussein, Iman M Aufi, Qasim Sharhan Al-Mayah

“…Nasopharyngeal swabs were collected from each participant and stored as frozen at -70 °C to use for RNA extraction and real time- polymerase chain reaction. Results: Out of all these samples, 54 samples were positive for respiratory syncytial virus (36%) and human metapneumovirus (1.33%). …”
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Genetic variability in Brazilian populations of Biomphalaria straminea complex detected by simple sequence repeat anchored polymerase chain reaction amplification by Roberta L Caldeira, Teofânia HDA Vidigal, Andrew JG Simpson, Omar S Carvalho

“…In the current work, we have standardized the simple sequence repeat anchored polymerase chain reaction (SSR-PCR) technique, using the primers (CA)8RY and K7, to study the genetic variability of these species. …”
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Comparison of polymerase chain reaction on fresh tissue samples and fecal drops on filter paper for detection of Trypanosoma cruzi in Rhodnius prolixus by PL Dorn, J Flores, B Brahney, A Gutierrez, R Rosales, A Rodas, C Monroy

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Absolute quantitative detection of genetically modified soybean MON87708×MON89788 with stacked traits by digital polymerase chain reaction by Junyi Xu, Xin Li, Jinglian Bai, Ying Liu, Shaojie Wang, Yueting Liu, Chunguang Yang

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Validation of a real-time polymerase chain reaction for the detection and quantification of the nucleic acid of Histoplasma from equine clinical samples by Lewis W. S. Fisher, Abdou Ceesay, Demba Jallow, Sophie F. Hawkes, Alicia Showering, Yaghouba Kane, Amadou Doumbia, Andrew P. Stringer, Claire E. Scantlebury

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Analysis of A Nosocomial Methicillin-Resistant Staphylococcus aureus Outbreak by Using Arbitrarily-Primed Polymerase Chain Reaction (AP-PCR) by Yeşim ÇETİNKAYA, Sesin KOCAGÖZ, Murat HAYRAN, Güler GÜRSU, Serhat ÜNAL

Subjects: “…Arbitrarily-primed polymerase chain reaction…”
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QPOLE: A Quick, Simple, and Cheap Alternative for POLE Sequencing in Endometrial Cancer by Multiplex Genotyping Quantitative Polymerase Chain Reaction by Anne Sophie V.M. Van den Heerik, Natalja T. Ter Haar, Lisa Vermij, Jan J. Jobsen, Mariel Brinkhuis, Suzan M. Roothaan, Alicia Leon-Castillo, Gitte Ortoft, Estrid Hogdall, Claus Hogdall, Tom Van Wezel, Ludy C.H.W. Lutgens, Marie A.D. Haverkort, Jas Khattra, Jessica N. McAlpine, Carien L. Creutzberg, Vincent T.H.B.M. Smit, C. Blake Gilks, Nanda Horeweg, Tjalling Bosse

“…To overcome this, we developed and validated a rapid, low-cost POLE hotspot test by a quantitative polymerase chain reaction (qPCR) assay, QPOLE.MATERIALS AND METHODSPrimer and fluorescence-labeled 5′-nuclease probe sequences of the 11 established pathogenic POLE mutations were designed. …”
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Detection of Respiratory Syncytial Virus and Human Metapneumovirus in Children with Respiratory Tract Infection by Use Real Time Polymerase Chain Reaction by Hala Luay Abduljabbar, Areej Atiyah Hussein, Iman M Aufi, Qasim Sharhan Al-Mayah

“…Nasopharyngeal swabs were collected from each participant and stored as frozen at -70 °C to use for RNA extraction and real time- polymerase chain reaction. Results: Out of all these samples, 54 samples were positive for respiratory syncytial virus (36%) and human metapneumovirus (1.33%). …”
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APLIKASI TEKNIK Polymerase Chain Reaction (PCR) UNTUK MENDETEKSI PEMALSUAN BAKSO SAPI YANG DICAMPUR DENGAN DAGING TIKUS by , ASTRI NUR FAIZAH, , Dr. drh. Yatri Drastini, M. Sc.

“…Results of PCR products were visualized by electrophoresis. Polymerase Chain Reaction product size for rat primer is 493 bp. …”

APLIKASI MULTIPLEX POLYMERASE CHAIN REACTION (m-PCR) UNTUK DETEKSI Mycobacterium tuberculosis DAN Mycobacterium bovis PADA DOMBA by , Yohanes Timbun Raja Mangihut Ronael Simarmata, , Prof. Dr. drh. Ida Tjahajati, MP.

Molecular Bird Sexing of Small Yellow-crested Cockatoo (Cacatua sulphurea, Gmelin 1788) Using Polymerase Chain Reaction Method by Ratri, Inggit Nindika Dianing, Rahmawati, Irhamna Putri, Nugrahani, Warih Pulung, Haryanto, Aris

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The Application of Polymerase Chain Reaction – Restriction Fragment Polymorphisms (PCR-RFLP) to Determine Genetic Diversity of Madura Cattle in Sapudi Island by Tety, Hartatik, Slamet, Diah volkandari, Sumadi, Sumadi, Widodo, Widodo

“…The aim of this study was to determine genetic diversity of Madura cattle using Polymerase Chain Reaction – Restriction Fragment Length Polymorphisms (PCR-RFLP) analysis of the cytochrome b (cytb) gene. …”
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