Comparison of Real-Time Polymerase Chain Reaction and pp65 Antigen Assay for Monitoring the Development of Cytomegalovirus Disease in Recipients of Kidney Transplant by Majid Komeijani, Mohammad Taghi Kardi, Seyyed Morteza Javadirad, Niloofar Naghshineh, Marzieh Rezaei, Simin Hemmati, Azadeh Hamzei Sichani

“…Keywords: Human cytomegalovirus, Kidney transplantation, pp65 protein, Real-time polymerase chain reaction…”
Get full text

Sodium Hypochlorite and Diode Laser in Non-Surgical Treatment of Periodontitis: Clinical and Bacteriological Study with Real Time Polymerase Chain Reaction (PCR) by Marwan El Mobadder, Samir Nammour, Jacek Matys, Kinga Grzech-Leśniak

“…A biological molecular test and real time polymerase chain reaction (RT-PCR) were performed before (T0) and after intervention (T1). …”
Get full text

Use of polymerase chain reaction and enzymatic cleavage in the identification of Helicobacter spp. in gastric mucosa of human beings from North Paraná, Brazil by PL Camargo, AA Alfieri, APFRL Bracarense, R Menoli, SR Spinosa, MK Hagiwara

Subjects: Get full text

Putative Internal Control Genes in Bovine Milk Small Extracellular Vesicles Suitable for Normalization in Quantitative Real Time-Polymerase Chain Reaction by Md. Matiur Rahman, Shigeo Takashima, Yuji O. Kamatari, Yassien Badr, Kaori Shimizu, Ayaka Okada, Yasuo Inoshima

“…Quantitative real-time polymerase chain reaction (qRT-PCR) is a widely used method for quantifying mRNA in tissues and cells. …”
Get full text

Infection of Infectious Spleen and Kidney Necrosis Virus and Viral Nervouse Necrosis in Seawater Fish Identifed Using Multiplex Polymerase Chain Reaction by Hidayati Kumalasari, Putu Eka Sudaryatma, Artanti Tri Lestari, Wahyu Nurlita, Wahyu Andy Nugraha, Nur Hasanah, Ida Ayu Mirah Meliana Dewi, Ni Putu Arya Shintya Anggraeni

Get full text

Comparison between culture and a multiplex quantitative real-time polymerase chain reaction assay detecting Ureaplasma urealyticum and U. parvum. by Maria Frølund, Eva Björnelius, Peter Lidbrink, Peter Ahrens, Jørgen Skov Jensen

“…A novel multiplex quantitative real-time polymerase chain reaction (qPCR) for simultaneous detection of U. urealyticum and U. parvum was developed and compared with quantitative culture in Shepard's 10 C medium for ureaplasmas in urethral swabs from 129 men and 66 women, and cervical swabs from 61 women. …”
Get full text

Antiviral Therapeutic Monitoring: Role of Hepatitis C Virus Core Antigen versus Hepatitis C Virus RNA by Polymerase Chain Reaction by Hajra Farooq, Muhammad Zaheer Iqbal, Waheed U Zamaan Tariq, Omar Rasheed Chughtai, Asad Ali Choudhry, Saadiya Mushtaq

Subjects: Get full text

Molecular diagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) by polymerase chain reaction-DNA sequencing of cerebrospinal fluids of patients by Praphathip Eamsobhana, Darawan Wanachiwanawin, Naowarat Dechkum, Anchana Parsartvit, Hoi Sen Yong

“…Cerebrospinal fluid (CSF) samples from clinically diagnosed patients with detectable Angiostrongylus canto-nensis-specific antibodies (n = 10), patients with clinically suspected cases that tested negative for A. cantonensis-an-tibodies (n = 5) and patients with cerebral gnathostomiasis (n = 2) and neurocysticercosis (n = 2) were examined by a single-step polymerase chain reaction (PCR) method using the AC primers for the 66-kDa native protein gene. …”
Get full text

Partial validation of a TaqMan quantitative polymerase chain reaction for the detection of the three genotypes of Infectious spleen and kidney necrosis virus by Samantha A. Koda, Kuttichantran Subramaniam, Paul M. Hick, Evelyn Hall, Thomas B. Waltzek, Joy A. Becker

Get full text

CapitalBio Mycobacterium real-time polymerase chain reaction detection test: Rapid diagnosis of Mycobacterium tuberculosis and nontuberculous mycobacterial infection by Yanqin Shen, Likui Fang, Xudong Xu, Bo Ye, Guocan Yu

Subjects: Get full text

Molecular identification of similar species of the genus Biomphalaria (Mollusca: Planorbidae) determined by a polymerase chain reaction-restriction fragment length polymorphism by Roberta Lima Caldeira, Teofânia HDA Vidigal, Sônia Torquato Paulinelli, Andrew JG Simpson, Omar S Carvalho

Subjects: Get full text

Comparison of polymerase chain reaction and next-generation sequencing with conventional urine culture for the diagnosis of urinary tract infections: A meta-analysis by Zhao Meng, Qi Shuang, Sun Yinuo, Zheng Xue

Subjects: Get full text

Partial validation of a TaqMan quantitative polymerase chain reaction for the detection of the three genotypes of Infectious spleen and kidney necrosis virus. by Samantha A Koda, Kuttichantran Subramaniam, Paul M Hick, Evelyn Hall, Thomas B Waltzek, Joy A Becker

Get full text

Comparative evaluation of the presence of Epstein-Barr virus in common leukoplakia, oral hairy leukoplakia and healthy mucosa by polymerase chain reaction (PCR) by Mohammad Reza Salehi, Faezeh Khozeimeh, Houshang Noshadian

Subjects: Get full text

A Multiplex Quantitative Polymerase Chain Reaction for the Rapid Differential Detection of Subgroups A, B, J, and K Avian Leukosis Viruses by Junfeng Dou, Zui Wang, Li Li, Qin Lu, Xinxin Jin, Xiaochun Ling, Zhengyu Cheng, Tengfei Zhang, Huabin Shao, Xinguo Zhai, Qingping Luo

“…In this study, a novel multiplex quantitative polymerase chain reaction (qPCR) assay was developed to detect ALV-A, ALV-B, ALV-J, and ALV-K with limits of detection of 40, 11, 13.7, and 96 copies/µL, respectively, and no cross-reactivity with other ALV subtypes and avian pathogens. …”
Get full text

Identification of Non-Tuberculosis Mycobacteria Isolates by Polymerase Chain Reaction–Restriction Enzyme Analysis of 360-bp Fragment of rpoB Gene by Shima Hadifar, Sharareh Moghim, Hajieh Gasemian-Safaei, Hosein Fazeli, Fariba Farid, Mohsen Moghoofei, Mansour Sedighi, Bahram Nasr-Esfahani

Subjects: “…Restriction Fragment Length Polymorphism Analysis of Polymerase Chain Reaction-Amplified Fragments (PCR-RFLP)…”
Get full text

Screening of High and Low-risk Human Papillomavirus Variants in Cervical Cancer Patients by Polymerase Chain Reaction: A Cross-sectional Study by Ritu Yadav, Lokesh Kadian, Smiti Nanda, Shalu Ranga, Parul Ahuja, Chetna Yadav, Preeti Chauhan

“…Detection of HPV infection and HPV genotyping (16, 18, 33, 58, 6, and 11) was done by using type-specific Polymerase Chain Reaction (PCR). Fisher’s exact test was applied to determine the association of high-risk HPV infection with other risk factors using GraphPad Prism Version 6 (La Jolla, California, USA). …”
Get full text

A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR by Stewart Don, Rutledge Robert G

Get full text

Rapid Diagnosis of Central Nervous System Scedosporiosis by Specific Quantitative Polymerase Chain Reaction Applied to Formalin-Fixed, Paraffin-Embedded Tissue by Robert J. Lauerer, Emely Rosenow, Rudi Beschorner, Johann-Martin Hempel, Georgios Naros, Anna Hofmann, Katharina Berger, Jennifer Sartor-Pfeiffer, Annerose Mengel, Ulf Ziemann, Volker Rickerts, Katharina Feil

“…In addition, histopathological differentiation from other filamentous fungi, including <i>Aspergillus fumigatus,</i> may not be possible, excluding early specific diagnosis and targeted therapy. Polymerase chain reaction (PCR) on tissue samples can rapidly identify fungi, leading to an earlier adequate treatment. …”
Get full text

Recovery of thermophilic campylobacter spp. in healthy and diarrhoeic pets by three culture methods and identification of the isolates by multiplex polymerase chain reaction (mpcr) by ABAY S, AYDIN F, HIZLISOY H, GÜNEŞ V

Get full text