Rapid Detection of Escherichia coli in Water Samples by Polymerase Chain Reaction using 16E1 and 16E2 Primers by Atiek Soemiati, Anglia Puspaningrum, Maksum R

“…The detection of Escherichia coli in various water samples by Polymerase Chain Reaction method using 16E1 and 16E2 primers has been carried out. …”
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Severe acute respiratory syndrome coronavirus 2 detection by real time polymerase chain reaction using pooling strategy of nasal samples by Annamaria Pratelli, Francesco Pellegrini, Luigi Ceci, Daniela Tatò, Maria Stella Lucente, Loredana Capozzi, Michele Camero, Alessio Buonavoglia

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Inter-laboratory ring trial to compare four quantitative polymerase chain reaction assays employed for detection of Mycobacterium avium subspecies paratuberculosis by L. Worsley, P. L. Davies

“…In Project 1, an independent performance comparison ring trail was conducted between three different commercial MAP quantitative polymerase chain reaction (qPCR) assay services (B, C, and D) currently marketed in Great Britain by three separate laboratories against each other and against a fourth assay (A) not available commercially in Great Britain. …”
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The Use of Multiplex Phosphorescence Analysis (PHOSPHAN^TM) and Polymerase Chain Reaction for Laboratory Diagnosis of Ixodid Tick-borne Borrelioses by T. I. Kuznetsova, V. G. Pomelova, E. I. Korenberg, N. S. Osin

“…In this report, we evaluated the performance of C6 peptide based multiplex Phosphorescence Analysis (PHOSPHANTM) and Polymerase Chain Reaction (nested PCR) for laboratory diagnosis of Ixodid Tick-borne Borrelioses (ITBB). …”
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A multiplex reverse transcription-nested polymerase chain reaction for detection and differentiation of wild-type and vaccine strains of canine distemper virus by Cui Shang-jin, Wang Zhao, Zhou Shun, Si Wei

“…<p>Abstract</p> <p>A multiplex reverse transcription-nested polymerase chain reaction (RT-nPCR) method was developed for the detection and differentiation of wild-type and vaccine strains of canine distemper virus (CDV). …”
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Multiplex Polymerase Chain Reaction/Pooled Antibiotic Susceptibility Testing Was Not Associated with Increased Antibiotic Resistance in Management of Complicated Urinary Tract Infections by Korman HJ, Mathur M, Luke N, Wang D, Zhao X, Levin M, Wenzler DL, Baunoch D

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Simple and rapid detection of common fetal aneuploidies using peptide nucleic acid probe-based real-time polymerase chain reaction by Subeen Hong, Seung Mi Lee, Sohee Oh, So Yeon Kim, Young Mi Jung, Sun Min Kim, Chan-Wook Park, Jong Kwan Jun, Byoung Jae Kim, Joong Shin Park

“…Abstract To examine the detection performance of a peptide nucleic acid (PNA) probe-based real-time time polymerase chain reaction (PCR) assay to detect common aneuploidies. …”
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METHOD FOR FMD VIRUS 146S COMPONENT CONCENTRATION DETERMINATION WITH REAL-TIME REVERSE TRANSCRIPTION – POLYMERASE CHAIN REACTION IN VACCINE RAW MATERIALS by M. I. Doronin, A. M. Timina, D. A. Lozovoy, V. A. Starikov, D. V. Mikhalishin, N. N. Medvedeva, A. V. Borisov

“…Method for determination of foot-and-mouth disease (FMD) virus 146S component concentration with real-time reverse transcription – polymerase chain reaction (rtRT-PCR) in vaccine raw materials is developed. …”
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Development of a one-step reverse transcription-quantitative polymerase chain reaction assay for the detection of porcine reproductive and respiratory syndrome virus. by Hansong Chae, Hyun Soo Roh, Young Mi Jo, Won Gyeong Kim, Jeong Byoung Chae, Seung-Uk Shin, Jung Won Kang

“…Despite the progress in developing diagnostic tools, including polymerase chain reaction (PCR)-based methods such as reverse transcription quantitative PCR (RT-qPCR) to diagnose PRRSV infection, its diagnosis at the genetic level is challenging because of its high genetic variability. …”
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Validation of Endogenous Control Genes by Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction for Acute Leukemia Gene Expression Studies by Flávia Melo Cunha de Pinho Pessoa, Vitória Beatriz de Jesus Viana, Marcelo Braga de Oliveira, Beatriz Maria Dias Nogueira, Rodrigo Monteiro Ribeiro, Deivide de Sousa Oliveira, Germison Silva Lopes, Ricardo Parente Garcia Vieira, Manoel Odorico de Moraes Filho, Maria Elisabete Amaral de Moraes, André Salim Khayat, Fabiano Cordeiro Moreira, Caroline Aquino Moreira-Nunes

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Protein mass spectrometry extends temporal blood meal detection over polymerase chain reaction in mouse-fed Chagas disease vectors by Judith I Keller, Justin O Schmidt, Anna M Schmoker, Bryan A Ballif, Lori Stevens

“…OBJECTIVES The purpose of this study was to determine blood meal detection ability as a function of method [polymerase chain reaction (PCR) vs. LC-MS/MS], time since feeding, and the effect of molting in mouse-fed triatomine insect vectors targeting hemoglobin and albumin proteins with LC-MS/MS and short interspersed nuclear elements (SINE)-based PCR. …”
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Identification of matB used as an endogenous reference gene for the qualitative and real‐time quantitative polymerase chain reaction detection of Lentinus edodes by Ling Li, Yuanmiao Wei, Yao Liu, Shuna Xiang, Hanyue Zhang, Ying Shang

“…The matB genes of L. edodes and 18 non‐L. edodes species were determined by qualitative polymerase chain reaction (PCR), but no amplification was found in non‐L. edodes species. …”
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Acute promyelocytic leukemia: the study of t(15;17) translocation by fluorescent in situ hybridization, reverse transcriptase-polymerase chain reaction and cytogenetic techniques by M.L.L.F. Chauffaille, M.S. Figueiredo, R. Beltrani, S.V. Antunes, M. Yamamoto, J. Kerbauy

“…The objective of the present study was to compare fluorescent in situ hybridization (FISH), reverse transcriptase-polymerase chain reaction (RT-PCR) and karyotyping in 18 samples (12 at diagnosis and 6 after treatment) from 13 AML M3 patients. …”
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Sexing of cattle embryos using RNA-sequencing data or polymerase chain reaction based on a complete sequence of cattle chromosome Y by Jada Lindsay Nix, Gustavo Pimenta Schettini, Fernando Henrique Biase

“…When necessary, RNA-sequencing data or polymerase chain reaction (PCR) assays can be used to determine the presence of the chromosome Y (ChrY) in samples. …”
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PPIA, HPRT1, and YWHAZ are suitable reference genes for quantitative polymerase chain reaction assay of the hypothalamic–pituitary–gonadal axis in sows by Hwan-Deuk Kim, Chan-Hee Jo, Yong-Ho Choe, Hyeon-Jeong Lee, Min Jang, Seul-Gi Bae, Sung-Ho Yun, Sung-Lim Lee, Gyu-Jin Rho, Seung-Joon Kim, Won-Jae Lee

Subjects: “…quantitative reverse transcription polymerase chain reaction…”
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Validation of Quantitative and Digital Polymerase Chain Reaction Assays Targeting the Mating Types of Phyllosticta citricarpa, the Causal Agent of Citrus Black Spot by Hector Urbina, Taylor Smith, Callie Jones, Xiaoan Sun, John McVay, Caroline Walker, Leroy Whilby, Greg Hodges, Trevor Smith

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Determination of helicobacter infections with 14c urea breath test (14c ubt) and polymerase chain reaction (pcr) in dogs and treatment by MERAL Y, GÖKALP G, UÇAK SEMİRGİN S, ÜNLÜ SÖĞÜT M

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Clinical evaluation of polymerase chain reaction coupled with quantum dot fluorescence analysis in the identification of bacteria and yeasts in patients with suspected bloodstream infections by Jie Li, Wenjia Fan, Xuehan Zou, Xuan Dai, Yueyue Zhao, Hongying Pan, Shijin Wu, Xi Li, Haijun Huang

“…Abstract Bloodstream infections are serious and complex infectious diseases that often require a rapid diagnosis. Polymerase chain reaction coupled with quantum dot fluorescence analysis (PCR‐QDFA) is a novel diagnostic technique. …”
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COMPLEX RESEARCH OF THE SPUTUM IN NEWLY DIAGNOSED PATIENTS WITH PULMONARY TUBERCULOSIS USING POLYMERASE CHAIN REACTION AND MYCROBIOLOGICAL CONTROL OF THE VIABILITY OF MYCOBACTERIUM TUBERCULOSIS by E.V. DUDENKO, S. SYDYKOVA

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GENETIC RELATEDNESS OF LOCAL VARIETIES OF RICE SOUTH SUMATRA BASED ON POLYMERASE CHAIN REACTION – RANDOM AMPLIFIED POLYMORPHIC DNA (PCR-RAPD) by Laila Hanum, Yuanita Windusari, Muharni, Fikri Adriansyah

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