Restoration of surfactant activity by polymyxin B in lipopolysaccharide-potentiated injury of immature rabbit lungs by Andrea Calkovska, Marie Haegerstrand-Björkman, Tore Curstedt

“…We evaluated the effect of surfactant therapy in combination with antibiotic polymyxin B (PxB) in double-hit model of neonatal lung injury. Surfactant (poractant alfa, Curosurf) was exposed to smooth (S) LPS without/with PxB and tested in captive bubble surfactometer. …”
Get full text

Physiological, Biochemical, and Biophysical Characterization of the Lung-Lavaged Spontaneously-Breathing Rabbit as a Model for Respiratory Distress Syndrome. by Francesca Ricci, Chiara Catozzi, Xabier Murgia, Brenda Rosa, Davide Amidani, Luca Lorenzini, Federico Bianco, Claudio Rivetti, Silvia Catinella, Gino Villetti, Maurizio Civelli, Barbara Pioselli, Carlo Dani, Fabrizio Salomone

“…Animals were randomized into two groups: Control group (nCPAP only) and InSurE group, consisting of a bolus of surfactant (Poractant alfa, 200 mg/kg) followed by nCPAP. Arterial blood gases were monitored until animal sacrifice, 3 hours post treatment. …”
Get full text

Comparison of LISA and INSURE Methods in Surfactant Application in Preterm Babies with Respiratory Distress Syndrome by Neşet Aydın, İbrahim Deger, Murat Başaranoğlu, Nihat Demir, Oğuz Tuncer

“…Sixty patients who needed surfactant replacement for respiratory distress syndrome were included in the study. Poractant alfa (200 mg/kg/dose) was given to all cases. …”
Get full text

Respiratory distress after ductus arteriosus ligation – Question by Vanessa Gorito, Gustavo Rocha, Elson Salgueiro, Paulo Éden Santos, Hercília Guimarães

“…The Apgar score was 4/8/8, needing positive pressure followed by nasal continuous airway positive pressure (NCPAP). One dose of poractant alfa was administered in the first hour of life. …”
Get full text

Lung Deposition of Surfactant Delivered via a Dedicated Laryngeal Mask Airway in Piglets by Anders Nord, Doris Cunha-Goncalves, Rikard Linnér, Federico Bianco, Fabrizio Salomone, Francesca Ricci, Marta Lombardini, Massimo Micaglio, Daniele Trevisanuto, Valeria Perez-de-Sa

“…All animals received 100 mg·kg⁻¹ of poractant alfa mixed with ^99mTechnetium-nanocolloid. …”
Get full text

Tracing exogenous surfactant in vivo in rabbits by the natural variation of 13C by Sonia Giambelluca, Francesca Ricci, Manuela Simonato, Luca Vedovelli, Umberto Traldi, Alessio Correani, Costanza Casiraghi, Matteo Storti, Arianna Mersanne, Paola Cogo, Fabrizio Salomone, Virgilio P. Carnielli

“…Rabbits were in nasal continuous positive airway pressure and treated with 0, 25, 50, 100 or 200 mg/kg of poractant alfa by InSurE. After 90 min, rabbits were depleted again and a new pool (BAL end experiment) was collected. …”
Get full text

Impact of the New Generation Reconstituted Surfactant CHF5633 on Human CD4+ Lymphocytes. by Markus Fehrholz, Kirsten Glaser, Silvia Seidenspinner, Barbara Ottensmeier, Tore Curstedt, Christian P Speer, Steffen Kunzmann

“…METHODS:Purified human CD4+ T cells were activated using anti CD3/CD28 antibodies and exposed to CHF5633, its components, or to the well-known animal-derived surfactant Poractant alfa (Curosurf®). Proliferative response and cell viability were assessed using flow cytometry and a methylthiazolyldiphenyltetrazolium bromide colorimetric assay. …”
Get full text

Effects of the New Generation Synthetic Reconstituted Surfactant CHF5633 on Pro- and Anti-Inflammatory Cytokine Expression in Native and LPS-Stimulated Adult CD14+ Monocytes. by Kirsten Glaser, Markus Fehrholz, Tore Curstedt, Steffen Kunzmann, Christian P Speer

“…METHODS:Highly purified adult CD14+ cells, either native or simultaneously stimulated with LPS, were exposed to CHF5633, its components, or poractant alfa (Curosurf®). Subsequent expression of TNF-α, IL-1β, IL-8 and IL-10 mRNA was quantified by real-time quantitative PCR, corresponding intracellular cytokine synthesis was analyzed by flow cytometry. …”
Get full text

Cerebral oxygenation and bioelectrical activity in preterm infants during surfactant replacement therapy with porcine and bovine preparations by Tomasz Szczapa, Łukasz Karpiński, Hanna Szczapa-Krenz, Beata Witosław, Aleksandra Adamczak, Jerzy Moczko, Izabela Miechowicz, Paweł Niedbalski, Marta Szymankiewicz-Bręborowicz, Jan Mazela

“…Material and methods Neonates born at 26–30 weeks of gestation with RDS requiring intubation and mechanical ventilation were randomized to SRT either with poractant alfa (A) or beractant (B). Saturation (SpO2), heart rate (HR), cerebral tissue oxygenation (StO2) and amplitude-integrated electroencephalography (aEEG) were simultaneously recorded prior to and up to 4 h after SRT. …”
Get full text

The Effect of Modified Porcine Surfactant Alone or in Combination with Polymyxin B on Lung Homeostasis in LPS-Challenged and Mechanically Ventilated Adult Rats by Maros Kolomaznik, Jana Kopincova, Zuzana Nova, Juliana Topercerova, Ivan Zila, Pavol Mikolka, Petra Kosutova, Katarina Matasova, Henrieta Skovierova, Marian Grendar, Daniela Mokra, Andrea Calkovska

“…Animals with LPS 500 μg/kg have been treated with exogenous surfactant (poractant alfa, Curosurf^®, 50 mg PL/kg b.w.) or surfactant with PxB 1% w.w. …”
Get full text

Comparison of the Efficacy of Three Natural Surfactants in Preterm Turkish Newborns with Respiratory Distress Syndrome by Fatma Hilal Yılmaz, Nuriye Tarakçı, Nazlı Dilay Gültekin, Mehmet Yücel, Ramazan Keçeci, Elif Nur Yıldırım Öztürk, Hüseyin Altunhan

“…The patients were divided into three groups, each of which received one of three surfactants: Group 1; beractant (100 mg/kg); group 2; poractant alfa (first dose of 200 mg/kg, recurrent doses of 100 mg/kg); group 3; calfactant (100 mg/kg). …”
Get full text

Outcomes at 7 Years of Age of Former Very Preterm Neonates with Repeated Surfactant Treatment for Prolonged Respiratory Distress in the Neonatal Period by Jean-Michel Hascoet, Hélène Deforge, Silvia Demoulin, Jean-Charles Picaud, Veronique Zupan, Isabelle Ligi, François Moreau, Aurelie Labarre, Patrick Daoud, Laurent Storme, Claude Bonabel, Isabelle Hamon

“…A repeated instillation of 200 mg/kg poractant alfa (SURF) did not improve early bronchopulmonary dysplasia, but the SURF infants needed less re-hospitalization than the controls for respiratory problems at 1- and 2-year PNA. …”
Get full text

Selected Abstracts of the 2nd Congress of joint European Neonatal Societies (jENS 2017); Venice (Italy); October 31-November 4, 2017; Session "Neonatal Pulmonology, Neonatal Respir... by --- Various Authors

“…CHARACTERIZATION OF PORACTANT ALFA AND BUDESONIDE EXTEMPORANEOUS COM­BINATION FOR SAFE AND EFFECTIVE INTRA­TRACHEAL ADMINISTRATION • F. …”
Get full text