Measuring in vivo protein turnover and exchange in yeast macromolecular assemblies by Zhanna Hakhverdyan, Kelly R. Molloy, Roman I. Subbotin, Javier Fernandez-Martinez, Brian T. Chait, Michael P. Rout

Subjects: “…Protein Biochemistry…”
Get full text

Protocol for evaluating drug-protein interactions based on fluorescence spectroscopy by Elaheh Jalali, Javad Sargolzaei

Subjects: Get full text

Protocol to reveal the binding partner of secreted housekeeping enzymes in Listeria monocytogenes via in silico prediction to in vivo validation by Dongqi Liu, Harrison D.B. Helmick, Jozef L. Kokini, Arun K. Bhunia

Subjects: Get full text

Characterization of antibody-antigen interactions using biolayer interferometry by Tal Noy-Porat, Ron Alcalay, Adva Mechaly, Eldar Peretz, Efi Makdasi, Ronit Rosenfeld, Ohad Mazor

Subjects: Get full text

A transcription-based approach to purify R-loop-containing plasmid DNA templates in vitro by Charanya Kumar, Dirk Remus

Subjects: Get full text

Protocol for in vitro phosphorylation of the MUS81-binding region of SLX4 using CDK1-cyclin B by Brandon J. Payliss, Haley D.M. Wyatt

Subjects: Get full text

Analyzing protein intermediate interactions in living E. coli cells using site-specific photo-crosslinking combined with chemical crosslinking by Ryoji Miyazaki, Yoshinori Akiyama

Subjects: Get full text

Protocol to measure protein-RNA binding using double filter-binding assays followed by phosphorimaging or high-throughput sequencing by Joel Vega-Badillo, Phillip D. Zamore, Karina Jouravleva

Subjects: Get full text

Protocol for real-time imaging of membrane fission by mitofissin by Tatsuro Maruyama, Nobuo N. Noda.

Subjects: Get full text

Protocol for using organic persulfides to measure the chemical reactivity of persulfide sensors by Joseph N. Fakhoury, Daiana A. Capdevila, David P. Giedroc

Subjects: “…Protein Biochemistry…”
Get full text

Protocol for quantifying N-Myc and global protein translation in neuroblastoma cells using click chemistry on polyvinylidene fluoride membranes by Pamorn Chittavanich, Duangporn Saengwimol, Ariestya Indah Permata Sari, Atthapol Srimongkol, Rossukon Kaewkhaw

Subjects: Get full text

Import mechanisms of pyocins G and AP41 by Atanaskovic, I

Subjects:

Protocol for determining protein dynamics using FT-IR spectroscopy by Hao Shen, Cuiping Fu, Junting Zhang, Bin Feng, Shaoning Yu

Subjects: Get full text

Two-color in vitro assay to visualize and quantify microtubule shaft dynamics by Mireia Andreu-Carbó, Simon Fernandes, Charlotte Aumeier

Subjects: Get full text

Identification of homologous protein models via 3D comparisons using predicted structures by Anyu Pan, Jieyi Shentu, Yangfan Zeng, Rong Guo, Yang Yu

Subjects: Get full text

In vitro and in cellulo ApoE particle formation, isolation, and characterization by Karina Lindner, Larissa C. van Ek, Khader Awwad, Franziska Hanke, Alla V. Korepanova, Vladimir Rybin, Katharina Beckenbauer, Anne-Claude Gavin

Subjects: Get full text

Protocol for hit-to-lead optimization of compounds by auto in silico ligand directing evolution (AILDE) approach by Longcan Mei, Fengxu Wu, Gefei Hao, Guangfu Yang

Subjects: Get full text

Protocol for preparing Thiopropyl Sepharose resin used for capturing S-nitrosylated proteins by Puneet Seth, Alfred Hausladen, Richard T. Premont, Jonathan S. Stamler

Subjects: Get full text

Protocol for reconstituting peptides/peptidomimetics from DMSO to aqueous buffers for circular dichroism analyses by William H. Deni, Tong Gao, Jinhua Wu

Subjects: Get full text

Protocol for a semi-quantitative approach to identify protein S-palmitoylation in cultured cells by acyl biotin exchange assay by Stuart Leishman, Najd M. Aljadeed, Paras K. Anand

Subjects: Get full text