Budding yeast RNA polymerases I and II employ parallel mechanisms of transcriptional termination. by Kawauchi, J, Mischo, H, Braglia, P, Rondon, A, Proudfoot, N

“…Both RNA polymerase I and II (Pol I and Pol II) in budding yeast employ a functionally homologous "torpedo-like" mechanism to promote transcriptional termination. …”

Mass spectrometry reveals stable modules in holo and apo RNA polymerases I and III. by Lane, L, Fernández-Tornero, C, Zhou, M, Morgner, N, Ptchelkine, D, Steuerwald, U, Politis, A, Lindner, D, Gvozdenovic, J, Gavin, A, Müller, C, Robinson, C

“…RNA polymerases are essential enzymes which transcribe DNA into RNA. …”

The SARS-CoV-2 RNA polymerase is a viral RNA capping enzyme by Walker, AP, Fan, H, Keown, JR, Knight, ML, Grimes, JM, Fodor, E

Transcription reinitiation by recycling RNA polymerase that diffuses on DNA after releasing terminated RNA by Kang, W, Ha, KS, Uhm, H, Park, K, Lee, JY, Hohng, S, Kang, C

“…Our single-molecule fluorescence study unveils that RNA transcript release precedes RNA polymerase (RNAP) dissociation from the DNA template much more often than their concurrent dissociations in intrinsic termination of bacterial transcription. …”

Regional specialization in human nuclei: visualization of discrete sites of transcription by RNA polymerase III. by Pombo, A, Jackson, D, Hollinshead, M, Wang, Z, Roeder, R, Cook, P

“…Mammalian nuclei contain three different RNA polymerases defined by their characteristic locations and drug sensitivities; polymerase I is found in nucleoli, and polymerases II and III in the nucleoplasm. …”

The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase. by Salgado, P, Makeyev, E, Butcher, S, Bamford, D, Stuart, D, Grimes, J

“…The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. …”

Structures of influenza A virus RNA polymerase offer insight into viral genome replication by Fan, H, Walker, A, Carrique, L, Keown, J, Serna Martin, I, Karia, D, Sharps, J, Hengrung, N, Pardon, E, Steyaert, J, Grimes, J, Fodor, E

“…Influenza A viruses contain a segmented negative-sense RNA genome, which is transcribed and replicated by the viral-RNA-dependent RNA polymerase (FluPolA) composed of PB1, PB2 and PA subunits3,4,5. …”

Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei. by Jackson, D, Iborra, F, Manders, E, Cook, P

“…Using HeLa cells, we have developed methods to determine 1) the number of RNA polymerases that are active at any moment, 2) the number of transcription sites, and 3) the number of polymerases associated with one transcription unit. …”

Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei by Jackson, D, Iborra, F, Manders, E, Cook, P

“…Using HeLa cells, we have developed methods to determine 1) the number of RNA polymerases that are active at any moment, 2) the number of transcription sites, and 3) the number of polymerases associated with one transcription unit. …”

Performance comparison between bootstrap and multiscale bootstrap for assessing phylogenetic tree for RNA polymerase by Safinah Sharuddin, Nora Muda

“…The aim of this study was to compare the performance between the AU value and BP value for assessing phylogenetic tree of RNA polymerase. The results have shown that multiscale bootstrap analysis can detect high sampling errors but not in bootstrap analysis. …”
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Ubiquitin proteomics identifies RNA polymerase I as a target of the Smc5/6 complex by Eva Ibars, Joan Codina-Fabra, Gemma Bellí, Celia Casas, Marc Tarrés, Roger Solé-Soler, Neus P. Lorite, Pilar Ximénez-Embún, Javier Muñoz, Neus Colomina, Jordi Torres-Rosell

“…In addition, our analysis suggests a connection between Nse1 and RNA polymerase I (RNA Pol I) ubiquitination. Specifically, Nse1 and the Smc5/6 complex promote ubiquitination of K408 and K410 in the clamp domain of Rpa190, a modification that induces its degradation in response to blocks in transcriptional elongation. …”
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Integrative analysis reveals histone demethylase LSD1 promotes RNA polymerase II pausing by Hani Jieun Kim, Pishun Li, Taiyun Kim, Andrew J. Oldfield, Xiaofeng Zheng, Pengyi Yang

“…Here, we report that LSD1 promotes RNA polymerase II (RNAPII) pausing, a rate-limiting step in transcription regulation, in ESCs. …”
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Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase by Juntaek Oh, Michiko Kimoto, Haoqing Xu, Jenny Chong, Ichiro Hirao, Dong Wang

“…T7 RNA polymerase (RNAP) is widely used for synthesizing RNA molecules with synthetic modifications and unnatural base pairs (UBPs). …”
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A New Insight into MYC Action: Control of RNA Polymerase II Methylation and Transcription Termination by Fiorella Scagnoli, Alessandro Palma, Annarita Favia, Claudio Scuoppo, Barbara Illi, Sergio Nasi

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Characterizing single-molecule dynamics of viral RNA-dependent RNA polymerases with multiplexed magnetic tweezers by Louis Kuijpers, Theo van Laar, Richard Janissen, Nynke H. Dekker

“…Summary: Multiplexed single-molecule magnetic tweezers (MT) have recently been employed to probe the RNA synthesis dynamics of RNA-dependent RNA polymerases (RdRp). Here, we present a protocol for simultaneously probing the RNA synthesis dynamics of hundreds of single polymerases with MT. …”
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MAF1, a repressor of RNA polymerase III-dependent transcription, regulates bone mass by Ellen Phillips, Naseer Ahmad, Li Sun, James Iben, Christopher J Walkey, Aleksandra Rusin, Tony Yuen, Clifford J Rosen, Ian M Willis, Mone Zaidi, Deborah L Johnson

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Virtual Screening and Molecular Docking Studies for Discovery of Potential RNA-Dependent RNA Polymerase Inhibitors by Mohammed Y. Ghazwani, Ahmed H. Bakheit, Abdulrahim R. Hakami, Hamad M. Alkahtani, Abdulrahman A. Almehizia

“…Since SARS-CoV-2 is a positive-strand RNA virus, its replication requires the viral RNA-dependent RNA polymerase (RdRp) enzyme. In this study, we report the discovery of new potential RdRp enzyme inhibitors based on computer modeling and simulation methodologies. …”
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Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules by Shodai Komatsu, Hirohisa Ohno, Hirohide Saito

“…Here to address these issues, we present a Target-dependent RNA polymerase (TdRNAP) that can induce RNA transcription in response to the intracellular target specifically recognized by single antibody. …”
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Structural bases of norovirus RNA dependent RNA polymerase inhibition by novel suramin-related compounds. by Romina Croci, Margherita Pezzullo, Delia Tarantino, Mario Milani, Shwu-Chen Tsay, Radhakrishnan Sureshbabu, Yi-Jin Tsai, Eloise Mastrangelo, Jacques Rohayem, Martino Bolognesi, Jih Ru Hwu

“…We previously identified Suramin (9) as a potent inhibitor of NV-RNA dependent RNA polymerase (NV-RdRp). Despite significant in vitro activities versus several pharmacological targets, Suramin clinical use is hampered by pharmacokinetics/toxicity problems. …”
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Cytoplasmic mRNA decay represses RNA polymerase II transcription during early apoptosis by Christopher Duncan-Lewis, Ella Hartenian, Valeria King, Britt A Glaunsinger

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