RNA-Dependent RNA Polymerase from Heterobasidion RNA Virus 6 Is an Active Replicase In Vitro by Alesia A. Levanova, Eeva J. Vainio, Jarkko Hantula, Minna M. Poranen

“…The purpose of the study was to determine the biochemical requirements for RNA synthesis catalyzed by HetRV6 RNA-dependent RNA polymerase (RdRp). HetRV6 RdRp was expressed in <i>Escherichia coli</i> and isolated to near homogeneity using liquid chromatography. …”
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RNA-Dependent RNA Polymerase of the Second Human Pegivirus Exhibits a High-Fidelity Feature by Shuyi Chen, Jiqin Wu, Xiaofeng Yang, Qiuli Sun, Su Liu, Farooq Rashid, Emmanuel Enoch Dzakah, Haiying Wang, Jufang Wang, Peng Gong, Shixing Tang

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Efficient cell-free expression with the endogenous <it>E. Coli </it>RNA polymerase and sigma factor 70 by Noireaux Vincent, Shin Jonghyeon

“…<p>Abstract</p> <p>Background</p> <p><it>Escherichia coli </it>cell-free expression systems use bacteriophage RNA polymerases, such as T7, to synthesize large amounts of recombinant proteins. …”
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Regulation of antimycin biosynthesis by the orphan ECF RNA polymerase sigma factor σAntA by Ryan F. Seipke, Elaine Patrick, Matthew I. Hutchings

“…Unusually for secondary metabolite clusters, the antG and antH promoters are regulated by an extracytoplasmic function (ECF) RNA polymerase sigma factor named σAntA which represents a new sub-family of ECF σ factors that is only found in antimycin producing strains. …”
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Structural basis of RNA polymerase II transcription on the chromatosome containing linker histone H1 by Rina Hirano, Haruhiko Ehara, Tomoya Kujirai, Tamami Uejima, Yoshimasa Takizawa, Shun-ichi Sekine, Hitoshi Kurumizaka

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Inhibition of Viral RNA-Dependent RNA Polymerases by Nucleoside Inhibitors: An Illustration of the Unity and Diversity of Mechanisms by Sailen Barik

“…RNA-dependent RNA polymerase (RdRP) is essential for the replication and expression of RNA viral genomes. …”
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Mutation in the RNA-Dependent RNA Polymerase of a Symbiotic Virus Is Associated With the Adaptability of the Viral Host by Hong Lu, Jing Li, Pengcheng Yang, Fei Jiang, Hongran Liu, Hongran Liu, Feng Cui, Feng Cui

“…One SNP at site 5,990, G5990A, located at the RNA-dependent RNA polymerase (RdRp) domain, demonstrated a predominance from G to A when the host aphids were shifted from V. faba to the low-fitness plants V. villosa or Medicago sativa. …”
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Condensin controls recruitment of RNA polymerase II to achieve nematode X-chromosome dosage compensation by William S Kruesi, Leighton J Core, Colin T Waters, John T Lis, Barbara J Meyer

“…Here we define TSSs and the distribution of transcriptionally engaged RNA polymerase II (Pol II) genome-wide in wild-type and dosage-compensation-defective animals to dissect this regulatory mechanism. …”
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A BIOINFORMATICS CHARACTERIZATION OF THE RNA-DEPENDENT RNA POLYMERASE FROM THE PARASITIC FUNGI, Metarhizium anisopliae by C. N. WEIR, E. EWONG, G. F. KING

“…However, many parasitic fungi posses a RNA-dependent RNA polymerase (RdRp) gene that serves as a defence against transgenes. …”
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Discovery of Potential Inhibitors for RNA-Dependent RNA Polymerase of Norovirus: Virtual Screening, and Molecular Dynamics by Oluwakemi Ebenezer, Maryam A. Jordaan, Nkululeko Damoyi, Michael Shapi

“…ORF1 encodes the nonstructural polyprotein that is cleaved into six nonstructural proteins, which include 3C-like cysteine protease (3CLpro) and a viral RNA-dependent RNA polymerase. ORF2 and ORF3 encode the proteins VP1 and VP2. …”
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A Conserved Deubiquitinating Enzyme Controls Cell Growth by Regulating RNA Polymerase I Stability by Lauren A. Richardson, Benjamin J. Reed, J. Michael Charette, Emily F. Freed, Eric K. Fredrickson, Melissa N. Locke, Susan J. Baserga, Richard G. Gardner

“…In particular, we discovered that the largest subunit of RNA polymerase I (RNAPI) is stabilized via Ubp10-mediated deubiquitination and that this is required in order to achieve optimal levels of ribosomes and cell growth. …”
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In Silico Analysis of Honey Bee Peptides as Potential Inhibitors of Capripoxvirus DNA-Directed RNA Polymerase by Ghulam Mustafa, Hafiza Salaha Mahrosh, Mahwish Salman, Muhammad Ali, Rawaba Arif, Sibtain Ahmed, Hossam Ebaid

“…The multiple sequence alignment using ClustalOmega revealed significant variations in the protein sequences of the DNA-dependent RNA polymerase of SPPV, GTPV, and LSDV. The three-dimensional structures of five selected bee peptides and DNA-directed RNA polymerase of SPPV, GTPV, and LSDV were predicted using trRosetta and I-TASSER and used for molecular docking and simulation studies. …”
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An Optimized Workflow for the Discovery of New Antimicrobial Compounds Targeting Bacterial RNA Polymerase Complex Formation by Alessia Caputo, Sara Sartini, Elisabetta Levati, Ilaria Minato, Gian Marco Elisi, Adriana Di Stasi, Catherine Guillou, Peter G. Goekjian, Pierre Garcia, David Gueyrard, Stéphane Bach, Arnaud Comte, Simone Ottonello, Silvia Rivara, Barbara Montanini

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Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription by Ália dos Santos, Daniel E. Rollins, Yukti Hari-Gupta, Hannah McArthur, Mingxue Du, Sabrina Yong Zi Ru, Kseniia Pidlisna, Ane Stranger, Faeeza Lorgat, Danielle Lambert, Ian Brown, Kevin Howland, Jesse Aaron, Lin Wang, Peter J. I. Ellis, Teng-Leong Chew, Marisa Martin-Fernandez, Alice L. B. Pyne, Christopher P. Toseland

“…We find that NDP52 clusters with RNA Polymerase II (RNAPII) at transcription initiation sites and that its overexpression promotes the formation of additional transcriptional clusters. …”
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Prdm5 regulates collagen gene transcription by association with RNA polymerase II in developing bone. by Giorgio Giacomo Galli, Kristian Honnens de Lichtenberg, Matteo Carrara, Wolfgang Hans, Manuela Wuelling, Bettina Mentz, Hinke Arnolda Multhaupt, Cathrine Kolster Fog, Klaus Thorleif Jensen, Juri Rappsilber, Andrea Vortkamp, Les Coulton, Helmut Fuchs, Valérie Gailus-Durner, Martin Hrabě de Angelis, Raffaele Adolfo Calogero, John Robert Couchman, Anders Henrik Lund

“…In particular, we show that Prdm5 controls both Collagen I transcription and fibrillogenesis by binding inside the Col1a1 gene body and maintaining RNA polymerase II occupancy. In vivo, Prdm5 loss results in delayed ossification involving a pronounced impairment in the assembly of fibrillar collagens. …”
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An NTP-driven mechanism for the nucleotide addition cycle of Escherichia coli RNA polymerase during transcription by Ronald S. Johnson, Mark Strausbauch, Christopher McCloud

“…The elementary steps of transcription as catalyzed by E. coli RNA polymerase during one and two rounds of the nucleotide addition cycle (NAC) were resolved in rapid kinetic studies. …”
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Novel <i>Escherichia coli</i> RNA Polymerase Binding Protein Encoded by Bacteriophage T5 by Evgeny Klimuk, Vladimir Mekler, Darya Lavysh, Marina Serebryakova, Natalia Akulenko, Konstantin Severinov

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Single molecules characterization of transcription of bacterial RNA-polymerase parameters using acoustic force spectroscopy by Arseniev Anatolii, Panfilov Mikhail, Pobegalov Georgii, Potyseva Alina, Pavlinova Polina, Yakunina Maria, Khodorkovskiy Mikhail

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Towards T7 RNA polymerase (T7RNAP)-based expression system in yeast: challenges and opportunities by David Sáez Moreno, Udi Qimron, Joana Azeredo, Lucília Domingues

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Structural basis of Ty1 integrase tethering to RNA polymerase III for targeted retrotransposon integration by Phong Quoc Nguyen, Sonia Huecas, Amna Asif-Laidin, Adrián Plaza-Pegueroles, Beatrice Capuzzi, Noé Palmic, Christine Conesa, Joël Acker, Juan Reguera, Pascale Lesage, Carlos Fernández-Tornero

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