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241
RNA-Dependent RNA Polymerase from Heterobasidion RNA Virus 6 Is an Active Replicase In Vitro
Published 2021-08-01“…The purpose of the study was to determine the biochemical requirements for RNA synthesis catalyzed by HetRV6 RNA-dependent RNA polymerase (RdRp). HetRV6 RdRp was expressed in <i>Escherichia coli</i> and isolated to near homogeneity using liquid chromatography. …”
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242
RNA-Dependent RNA Polymerase of the Second Human Pegivirus Exhibits a High-Fidelity Feature
Published 2022-10-01Subjects: Get full text
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243
Efficient cell-free expression with the endogenous <it>E. Coli </it>RNA polymerase and sigma factor 70
Published 2010-06-01“…<p>Abstract</p> <p>Background</p> <p><it>Escherichia coli </it>cell-free expression systems use bacteriophage RNA polymerases, such as T7, to synthesize large amounts of recombinant proteins. …”
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244
Regulation of antimycin biosynthesis by the orphan ECF RNA polymerase sigma factor σAntA
Published 2014-02-01“…Unusually for secondary metabolite clusters, the antG and antH promoters are regulated by an extracytoplasmic function (ECF) RNA polymerase sigma factor named σAntA which represents a new sub-family of ECF σ factors that is only found in antimycin producing strains. …”
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245
Structural basis of RNA polymerase II transcription on the chromatosome containing linker histone H1
Published 2022-11-01Get full text
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246
Inhibition of Viral RNA-Dependent RNA Polymerases by Nucleoside Inhibitors: An Illustration of the Unity and Diversity of Mechanisms
Published 2022-10-01“…RNA-dependent RNA polymerase (RdRP) is essential for the replication and expression of RNA viral genomes. …”
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247
Mutation in the RNA-Dependent RNA Polymerase of a Symbiotic Virus Is Associated With the Adaptability of the Viral Host
Published 2022-03-01“…One SNP at site 5,990, G5990A, located at the RNA-dependent RNA polymerase (RdRp) domain, demonstrated a predominance from G to A when the host aphids were shifted from V. faba to the low-fitness plants V. villosa or Medicago sativa. …”
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248
Condensin controls recruitment of RNA polymerase II to achieve nematode X-chromosome dosage compensation
Published 2013-06-01“…Here we define TSSs and the distribution of transcriptionally engaged RNA polymerase II (Pol II) genome-wide in wild-type and dosage-compensation-defective animals to dissect this regulatory mechanism. …”
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249
A BIOINFORMATICS CHARACTERIZATION OF THE RNA-DEPENDENT RNA POLYMERASE FROM THE PARASITIC FUNGI, Metarhizium anisopliae
Published 2016-05-01“…However, many parasitic fungi posses a RNA-dependent RNA polymerase (RdRp) gene that serves as a defence against transgenes. …”
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250
Discovery of Potential Inhibitors for RNA-Dependent RNA Polymerase of Norovirus: Virtual Screening, and Molecular Dynamics
Published 2020-12-01“…ORF1 encodes the nonstructural polyprotein that is cleaved into six nonstructural proteins, which include 3C-like cysteine protease (3CLpro) and a viral RNA-dependent RNA polymerase. ORF2 and ORF3 encode the proteins VP1 and VP2. …”
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251
A Conserved Deubiquitinating Enzyme Controls Cell Growth by Regulating RNA Polymerase I Stability
Published 2012-08-01“…In particular, we discovered that the largest subunit of RNA polymerase I (RNAPI) is stabilized via Ubp10-mediated deubiquitination and that this is required in order to achieve optimal levels of ribosomes and cell growth. …”
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252
In Silico Analysis of Honey Bee Peptides as Potential Inhibitors of Capripoxvirus DNA-Directed RNA Polymerase
Published 2023-07-01“…The multiple sequence alignment using ClustalOmega revealed significant variations in the protein sequences of the DNA-dependent RNA polymerase of SPPV, GTPV, and LSDV. The three-dimensional structures of five selected bee peptides and DNA-directed RNA polymerase of SPPV, GTPV, and LSDV were predicted using trRosetta and I-TASSER and used for molecular docking and simulation studies. …”
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253
An Optimized Workflow for the Discovery of New Antimicrobial Compounds Targeting Bacterial RNA Polymerase Complex Formation
Published 2022-10-01Subjects: Get full text
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254
Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription
Published 2023-05-01“…We find that NDP52 clusters with RNA Polymerase II (RNAPII) at transcription initiation sites and that its overexpression promotes the formation of additional transcriptional clusters. …”
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255
Prdm5 regulates collagen gene transcription by association with RNA polymerase II in developing bone.
Published 2012-01-01“…In particular, we show that Prdm5 controls both Collagen I transcription and fibrillogenesis by binding inside the Col1a1 gene body and maintaining RNA polymerase II occupancy. In vivo, Prdm5 loss results in delayed ossification involving a pronounced impairment in the assembly of fibrillar collagens. …”
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256
An NTP-driven mechanism for the nucleotide addition cycle of Escherichia coli RNA polymerase during transcription
Published 2022-01-01“…The elementary steps of transcription as catalyzed by E. coli RNA polymerase during one and two rounds of the nucleotide addition cycle (NAC) were resolved in rapid kinetic studies. …”
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257
Novel <i>Escherichia coli</i> RNA Polymerase Binding Protein Encoded by Bacteriophage T5
Published 2020-07-01Subjects: Get full text
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258
Single molecules characterization of transcription of bacterial RNA-polymerase parameters using acoustic force spectroscopy
Published 2022-03-01Subjects: Get full text
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259
Towards T7 RNA polymerase (T7RNAP)-based expression system in yeast: challenges and opportunities
Published 2022-12-01Subjects: Get full text
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260
Structural basis of Ty1 integrase tethering to RNA polymerase III for targeted retrotransposon integration
Published 2023-03-01Get full text
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