CDK9 and PP2A regulate RNA polymerase II transcription termination and coupled RNA maturation by Tellier, M, Zaborowska, J, Neve, J, Nojima, T, Hester, S, Fournier, M, Furger, A, Murphy, S

“…CDK9 is a kinase critical for the productive transcription of protein-coding genes by RNA polymerase II (pol II). As part of P-TEFb, CDK9 phosphorylates the carboxyl-terminal domain (CTD) of pol II and elongation factors, which allows pol II to elongate past the early elongation checkpoint (EEC) encountered soon after initiation. …”

Mutational analysis of the PA and PB2 subunits of the influenza RNA polymerase complex leads to new insights into function by Fodor, E, Fechter, P, Crow, M, Deng, T, Mingay, L, Sharps, J, Brownlee, G

“…The RNA-dependent RNA polymerase complex of influenza A virus consists of three subunits, PB1, PB2 and PA. …”

Design and use of Chikungunya virus replication templates utilizing mammalian and mosquito RNA polymerase I mediated transcription by Utt, A, Rausalu, K, Jakobson, M, Männik, A, Alphey, L, Fragkoudis, R, Merits, A

“…In contrast, RNA polymerase I promoters from <em>Aedes</em> mosquitoes exhibited strong species specificity. …”

Plasmodium falciparum var gene expression is developmentally controlled at the level of RNA polymerase II-mediated transcription initiation. by Kyes, S, Christodoulou, Z, Pinches, R, Kriek, N, Horrocks, P, Newbold, C

“…We demonstrate here that exclusive var gene expression is controlled at the level of transcription initiation during ring stages and that var genes are transcribed by RNA polymerase II. This represents another example where P. falciparum differs from the paradigm for antigenic variation, Trypanosoma brucei.…”

RNA polymerase clamp conformational dynamics: long-lived states and modulation by crowding, cations, and nonspecific DNA binding by Mazumder, A, Wang, A, Uhm, H, Ebright, RH, Kapanidis, AN

“…The RNA polymerase (RNAP) clamp, a mobile structural element conserved in RNAP from all domains of life, has been proposed to play critical roles at different stages of transcription. …”

Archaeal RNA polymerase: the influence of the protruding stalk in crystal packing and preliminary biophysical analysis of the Rpo13 subunit. by Wojtas, M, Peralta, B, Ondiviela, M, Mogni, M, Bell, S, Abrescia, N

“…We review recent results on the complete structure of the archaeal RNAP (RNA polymerase) enzyme of Sulfolobus shibatae. We compare the three crystal forms in which this RNAP packs (space groups P2₁2₁2₁, P2₁2₁2 and P2₁) and provide a preliminary biophysical characterization of the newly identified 13-subunit Rpo13. …”

Repurposing of potential antiviral drugs against RNA-dependent RNA polymerase of SARS-CoV-2 by computational approach by Sivakumar Gangadharan, Jenifer Mallavarpu Ambrose, Anusha Rajajagadeesan, Malathi Kullappan, Shankargouda Patil, Sri Harshini Gandhamaneni, Vishnu Priya Veeraraghavan, Aruna Kumari Nakkella, Alok Agarwal, Selvaraj Jayaraman, Krishna Mohan Surapaneni

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Global Genomic Analysis of SARS-CoV-2 RNA Dependent RNA Polymerase Evolution and Antiviral Drug Resistance by Alfredo Mari, Tim Roloff, Madlen Stange, Kirstine K. Søgaard, Erblin Asllanaj, Gerardo Tauriello, Leila Tamara Alexander, Michael Schweitzer, Karoline Leuzinger, Alexander Gensch, Aurélien E. Martinez, Julia Bielicki, Hans Pargger, Martin Siegemund, Christian H. Nickel, Roland Bingisser, Michael Osthoff, Stefano Bassetti, Parham Sendi, Manuel Battegay, Catia Marzolini, Helena M. B. Seth-Smith, Torsten Schwede, Hans H. Hirsch, Adrian Egli

“…Currently, the SARS-CoV-2 RNA-dependent RNA polymerase (RdRp, encoded by <i>nsp12-nsp7-nsp8</i>) has been targeted by numerous inhibitors, e.g., remdesivir, the only provisionally approved treatment to-date, although the clinical impact of these interventions remains inconclusive. …”
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Genistein is effective in inhibiting Orf virus infection in vitro by targeting viral RNA polymerase subunit RPO30 protein by Pin Lv, Pin Lv, Ziyu Fang, Jiyu Guan, Lijun Lv, Mengshi Xu, Xingyuan Liu, Zhuomei Li, Yungang Lan, Zi Li, Huijun Lu, Deguang Song, Wenqi He, Feng Gao, Dacheng Wang, Dacheng Wang, Kui Zhao

“…Poxviruses encode a multisubunit DNA-dependent RNA polymerase (vRNAP) that carries out viral gene expression in the host cytoplasm, which is a viral factor essential to poxvirus replication. …”
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PARP1′s Involvement in RNA Polymerase II Elongation: Pausing and Releasing Regulation through the Integrator and Super Elongation Complex by Elena A. Matveeva, Hejer Dhahri, Yvonne Fondufe-Mittendorf

“…RNA polymerase elongation along the gene body is tightly regulated to ensure proper transcription and alternative splicing events. …”
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Looking for inhibitors of the dengue virus NS5 RNA-dependent RNA-polymerase using a molecular docking approach by Galiano V, Garcia-Valtanen P, Micol V, Encinar JA

Subjects: “…NS5 RNA-dependent RNA-polymerase…”
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Reprogramming mRNA Expression in Response to Defect in RNA Polymerase III Assembly in the Yeast <i>Saccharomyces cerevisiae</i> by Izabela Rudzińska, Małgorzata Cieśla, Tomasz W. Turowski, Alicja Armatowska, Ewa Leśniewska, Magdalena Boguta

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Sofosbuvir Selects for Drug-Resistant Amino Acid Variants in the Zika Virus RNA-Dependent RNA-Polymerase Complex In Vitro by Adele Boccuto, Filippo Dragoni, Francesca Picarazzi, Alessia Lai, Carla Della Ventura, Carla Veo, Federica Giammarino, Francesco Saladini, Gianguglielmo Zehender, Maurizio Zazzi, Mattia Mori, Ilaria Vicenti

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Heat Treatment Promotes Ubiquitin-Mediated Proteolysis of SARS-CoV-2 RNA Polymerase and Decreases Viral Load by Yasen Maimaitiyiming, Tao Yang, Qian Qian Wang, Yan Feng, Zhi Chen, Mikael Björklund, Fudi Wang, Chonggao Hu, Chih-Hung Hsu, Hua Naranmandura

“…Here, we reveal a potential mechanism by which mild heat treatment destabilizes the wild-type RNA-dependent RNA polymerase (also known as nonstructural protein 12 (NSP12)) of SARS-CoV-2 as well as the P323L mutant commonly found in SARS-CoV-2 variants, including omicron and IHU. …”
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Binding of GS-461203 and Its Halogen Derivatives to HCV Genotype 2a RNA Polymerase Drug Resistance Mutants by Muhammad Arba, Setyanto Tri Wahyudi, Muhammad Sulaiman Zubair, Dylan Brunt, Mursalin Singh, Chun Wu

“…Hepatitis C Virus (HCV) is reported to develop GS-461203 resistance because of multiple mutations within the RNA-dependent RNA Polymerase (RdRp) of HCV. The lack of a high-resolution structure of these RdRp mutants in complex with GS-461203 hinders efforts to understand the drug resistance. …”
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Rifampicin exerts anti-mucoviscous activity against hypervirulent Klebsiella pneumoniae via binding to the RNA polymerase β subunit by Mitsunori Tohda, Ken-Ichi Oinuma, Arata Sakiyama, Taishi Tsubouchi, Mamiko Niki, Hiroki Namikawa, Kenshi Yamane, Koichi Yamada, Tetsuya Watanabe, Kazuhisa Asai, Hiroshi Kakeya, Yukihiro Kaneko, Tomoya Kawaguchi

“…We previously discovered that rifampicin (RFP), a bactericidal drug that binds to and inhibits the β subunit of RNA polymerase (RpoB), elicits anti-mucoviscous activity against hvKP by suppressing rmpA, a regulator of capsule production. …”
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Structural and Functional Insight into the Mechanism of <i>Bacillus subtilis</i> 6S-1 RNA Release from RNA Polymerase by Sweetha Ganapathy, Philipp G. Hoch, Marcus Lechner, Malte Bussiek, Roland K. Hartmann

“…Here we investigated the refolding of <i>Bacillus subtilis</i> 6S-1 RNA and its release from σ^A-RNA polymerase (σ^A-RNAP) in vitro using truncated and mutated 6S-1 RNA variants. …”
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HSV-1 ICP22 Is a Selective Viral Repressor of Cellular RNA Polymerase II-Mediated Transcription Elongation by Nur Firdaus Isa, Olivier Bensaude, Nadiah C. Aziz, Shona Murphy

“…ICP22 inhibits phosphorylation of Ser2 of the RNA polymerase II (pol II) carboxyl-terminal domain (CTD) and productive elongation of pol II. …”
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Investigation of Multi-Subunit <i>Mycobacterium tuberculosis</i> DNA-Directed RNA Polymerase and Its Rifampicin Resistant Mutants by Mokgerwa Zacharia Monama, Fisayo Olotu, Özlem Tastan Bishop

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Dynamics of RNA Polymerase II Pausing and Bivalent Histone H3 Methylation during Neuronal Differentiation in Brain Development by Jiancheng Liu, Xiwei Wu, Heying Zhang, Gerd P. Pfeifer, Qiang Lu

“…During cellular differentiation, genes important for differentiation are expected to be silent in stem/progenitor cells yet can be readily activated. RNA polymerase II (Pol II) pausing and bivalent chromatin marks are two paradigms suited for establishing such a poised state of gene expression; however, their specific contributions in development are not well understood. …”
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