The N-Terminal α-Helix of Potato Virus X-Encoded RNA-Dependent RNA Polymerase Is Required for Membrane Association and Multimerization by Xue Jiang, Yameng Luan, Mengzhu Chai, Yingshuai Yang, Yuting Wang, Wenjia Deng, Yonggang Li, Xiaofei Cheng, Xiaoyun Wu

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LGP2 directly interacts with flavivirus NS5 RNA-dependent RNA polymerase and downregulates its pre-elongation activities. by Zhongyuan Tan, Jiqin Wu, Li Huang, Ting Wang, Zhenhua Zheng, Jianhui Zhang, Xianliang Ke, Yuan Zhang, Yan Liu, Hanzhong Wang, Jianping Tao, Peng Gong

“…Co-immunoprecipitation (Co-IP) and confocal immunofluorescence data suggest that LGP2 likely colocalizes with the replication complex (RC) of ZIKV by interacting with viral RNA-dependent RNA polymerase (RdRP) NS5. We further verify that the regulatory domain (RD) of LGP2 directly interacts with RdRP of NS5 by biolayer interferometry assay. …”
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RNA Overwriting of Cellular mRNA by Cas13b-Directed RNA-Dependent RNA Polymerase of Influenza A Virus by Shinzi Ogasawara, Sae Ebashi

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Characterization of the RNA-dependent RNA polymerase from Chikungunya virus and discovery of a novel ligand as a potential drug candidate by Marjorie C. L. C. Freire, Luis G. M. Basso, Luis F. S. Mendes, Nathalya C. M. R. Mesquita, Melina Mottin, Rafaela S. Fernandes, Lucca R. Policastro, Andre S. Godoy, Igor A. Santos, Uriel E. A. Ruiz, Icaro P. Caruso, Bruna K. P. Sousa, Ana C. G. Jardim, Fabio C. L. Almeida, Laura H. V. G. Gil, Carolina H. Andrade, Glaucius Oliva

“…The RNA-dependent RNA-polymerase (nsP4-CHIKV) is considered the key enzyme of the CHIKV replication complex and a suitable target for antiviral therapy. …”
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Isolation and characterization of <it>Ehrlichia chaffeensis </it>RNA polymerase and its use in evaluating p28 outer membrane protein gene promoters by Peddireddi Lalitha, Liu Huitao, Faburay Bonto, Ganta Roman R

“…This is the first report of the functional characterization of <it>E. chaffeensis </it>RNA polymerase and <it>in vitro </it>mapping of the pathogen promoters using the enzyme. …”
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Tissue-specific RNA Polymerase II promoter-proximal pause release and burst kinetics in a Drosophila embryonic patterning network by George Hunt, Roshan Vaid, Sergei Pirogov, Alexander Pfab, Christoph Ziegenhain, Rickard Sandberg, Johan Reimegård, Mattias Mannervik

“…Results Here we comprehensively resolve differential transcriptional and chromatin states during Drosophila DV patterning. We find that RNA Polymerase II pausing is established at DV promoters prior to zygotic genome activation (ZGA), that pausing persists irrespective of cell fate, but that release into productive elongation is tightly regulated and accompanied by tissue-specific P-TEFb recruitment. …”
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Psychrophilic phage VSW-3 RNA polymerase reduces both terminal and full-length dsRNA byproducts in in vitro transcription by Heng Xia, Bingbing Yu, Yixin Jiang, Rui Cheng, Xueling Lu, Hui Wu, Bin Zhu

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Translocation pause of remdesivir-containing primer/template RNA duplex within SARS-CoV-2’s RNA polymerase complexes by Yuanjun Shi, Jimin Wang, Victor S. Batista

“…The mechanism of remdesivir incorporation into the RNA primer by the RNA-dependent RNA polymerase (RdRp) of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) remains to be fully established at the molecular level. …”
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Type B and type A influenza polymerases have evolved distinct binding interfaces to recruit the RNA polymerase II CTD. by Tim Krischuns, Catherine Isel, Petra Drncova, Maria Lukarska, Alexander Pflug, Sylvain Paisant, Vincent Navratil, Stephen Cusack, Nadia Naffakh

“…The virus-encoded RNA-dependent RNA polymerases (FluPolB and FluPolA) are PB1-PB2-PA heterotrimers that perform transcription and replication of the viral genome in the nucleus of infected cells. …”
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IE1 of Autographa californica Multiple Nucleopolyhedrovirus Activates Low Levels of Late Gene Expression in the Absence of Virus RNA Polymerase by Yong Qi, Shan-Shan Wang, Lu-Lin Li

“…ABSTRACT Early and late gene expressions of baculoviruses have been known to rely on host RNA polymerase II and a virus-encoded RNA polymerase, separately. …”
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Virtual Combinatorial Library Screening of Quinadoline B Derivatives against SARS-CoV-2 RNA-Dependent RNA Polymerase by Simone Brogi, Mark Tristan Quimque, Kin Israel Notarte, Jeremiah Gabriel Africa, Jenina Beatriz Hernandez, Sophia Morgan Tan, Vincenzo Calderone, Allan Patrick Macabeo

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The RNA Polymerase Inhibitor Corallopyronin A Has a Lower Frequency of Resistance Than Rifampicin in <i>Staphylococcus aureus</i> by Jan Balansky, Kenneth Pfarr, Christiane Szekat, Stefan Kehraus, Tilman Aden, Miriam Grosse, Rolf Jansen, Thomas Hesterkamp, Andrea Schiefer, Gabriele M. König, Marc Stadler, Achim Hoerauf, Gabriele Bierbaum

“…Corallopyronin A (CorA) is active against Gram-positive bacteria and targets the switch region of RNA polymerase. Because of the high frequency of mutation (FoM) leading to rifampicin resistance, we determined the CorA FoM in <i>S. aureus</i> using fluctuation analysis at 4 × minimum inhibitory concentration (MIC). …”
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Inhibition of the RNA-Dependent RNA-Polymerase from SARS-CoV‑2 by 6‑Chloropurine Isoxazoline-Carbocyclic Monophosphate Nucleotides by Marco Leusciatti, Beatrice Macchi, Francesca Marino-Merlo, Antonio Mastino, Giulia Morra, Paolo Quadrelli

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Genome-wide analysis in Plasmodium falciparum reveals early and late phases of RNA polymerase II occupancy during the infectious cycle by Rai, Ragini, Zhu, Lei, Chen, Haifen, Gupta, Archana Patkar, Sze, Siu Kwan, Zheng, Jie, Ruedl, Christiane, Bozdech, Zbynek, Featherstone, Mark

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Towards the design of drugs against the dengue virus RNA dependent RNA polymerase : mechanistic studies of enzyme activities and substrate binding. by Koh, Jolene Hong Kiew.

“…Dengue NS5 consists of 2 domains- methyltransferase and RNA-dependent RNA polymerase (RdRp), interspersed by a 9 amino acid linker. …”
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The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II by Jeon, Choon Ju, Yoon, Ho Sup, Agarwal, Kan

“…The eukaryotic transcription factor TFIIS enhances elongation and nascent transcript cleavage activities of RNA polymerase II in a stalled elongation complex. By site-directed mutagenesis, we have demonstrated that invariant residues Asp-261 and Glu-262 of the nucleic acid-binding TFIIS Zn ribbon are critical for stimulation of both elongation and RNA cleavage activities of RNA polymerase II. …”
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Binding to DNA of the RNA-polymerase II C-terminal domain allows discrimination between Cdk7 and Cdk9 phosphorylation by Lolli, G

“…The C-terminal domain (CTD) of RNA polymerase II regulates transcription through spatially and temporally coordinated events. …”

Gene silencing pathway RNA-dependent RNA polymerase of Neurospora crassa: yeast expression and crystallization of selenomethionated QDE-1 protein. by Laurila, MR, Salgado, P, Makeyev, E, Nettelship, J, Stuart, D, Grimes, J, Bamford, D

“…The RNA-dependent RNA polymerase, QDE-1, is a component of the RNA silencing pathway in Neurospora crassa. …”

A single amino acid mutation in the PA subunit of the influenza virus RNA polymerase inhibits endonucleolytic cleavage of capped RNAs. by Fodor, E, Crow, M, Mingay, L, Deng, T, Sharps, J, Fechter, P, Brownlee, G

“…The influenza A virus RNA-dependent RNA polymerase consists of three subunits-PB1, PB2, and PA. …”

Zn(2+) inhibits coronavirus and arterivirus RNA polymerase activity in vitro and zinc ionophores block the replication of these viruses in cell culture by Te Velthuis, A, van den Worm, S, Sims, A, Baric, R, Snijder, E, van Hemert, M

“…The RNA synthesis of these two distantly related nidoviruses is catalyzed by an RNA-dependent RNA polymerase (RdRp), which is the core enzyme of their multiprotein replication and transcription complex (RTC). …”