-
441
The N-Terminal α-Helix of Potato Virus X-Encoded RNA-Dependent RNA Polymerase Is Required for Membrane Association and Multimerization
Published 2022-08-01Subjects: Get full text
Article -
442
LGP2 directly interacts with flavivirus NS5 RNA-dependent RNA polymerase and downregulates its pre-elongation activities.
Published 2023-09-01“…Co-immunoprecipitation (Co-IP) and confocal immunofluorescence data suggest that LGP2 likely colocalizes with the replication complex (RC) of ZIKV by interacting with viral RNA-dependent RNA polymerase (RdRP) NS5. We further verify that the regulatory domain (RD) of LGP2 directly interacts with RdRP of NS5 by biolayer interferometry assay. …”
Get full text
Article -
443
RNA Overwriting of Cellular mRNA by Cas13b-Directed RNA-Dependent RNA Polymerase of Influenza A Virus
Published 2023-06-01Subjects: Get full text
Article -
444
Characterization of the RNA-dependent RNA polymerase from Chikungunya virus and discovery of a novel ligand as a potential drug candidate
Published 2022-06-01“…The RNA-dependent RNA-polymerase (nsP4-CHIKV) is considered the key enzyme of the CHIKV replication complex and a suitable target for antiviral therapy. …”
Get full text
Article -
445
Isolation and characterization of <it>Ehrlichia chaffeensis </it>RNA polymerase and its use in evaluating p28 outer membrane protein gene promoters
Published 2011-04-01“…This is the first report of the functional characterization of <it>E. chaffeensis </it>RNA polymerase and <it>in vitro </it>mapping of the pathogen promoters using the enzyme. …”
Get full text
Article -
446
Tissue-specific RNA Polymerase II promoter-proximal pause release and burst kinetics in a Drosophila embryonic patterning network
Published 2024-01-01“…Results Here we comprehensively resolve differential transcriptional and chromatin states during Drosophila DV patterning. We find that RNA Polymerase II pausing is established at DV promoters prior to zygotic genome activation (ZGA), that pausing persists irrespective of cell fate, but that release into productive elongation is tightly regulated and accompanied by tissue-specific P-TEFb recruitment. …”
Get full text
Article -
447
Psychrophilic phage VSW-3 RNA polymerase reduces both terminal and full-length dsRNA byproducts in in vitro transcription
Published 2022-12-01Subjects: Get full text
Article -
448
Translocation pause of remdesivir-containing primer/template RNA duplex within SARS-CoV-2’s RNA polymerase complexes
Published 2022-10-01“…The mechanism of remdesivir incorporation into the RNA primer by the RNA-dependent RNA polymerase (RdRp) of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) remains to be fully established at the molecular level. …”
Get full text
Article -
449
Type B and type A influenza polymerases have evolved distinct binding interfaces to recruit the RNA polymerase II CTD.
Published 2022-05-01“…The virus-encoded RNA-dependent RNA polymerases (FluPolB and FluPolA) are PB1-PB2-PA heterotrimers that perform transcription and replication of the viral genome in the nucleus of infected cells. …”
Get full text
Article -
450
IE1 of Autographa californica Multiple Nucleopolyhedrovirus Activates Low Levels of Late Gene Expression in the Absence of Virus RNA Polymerase
Published 2023-02-01“…ABSTRACT Early and late gene expressions of baculoviruses have been known to rely on host RNA polymerase II and a virus-encoded RNA polymerase, separately. …”
Get full text
Article -
451
Virtual Combinatorial Library Screening of Quinadoline B Derivatives against SARS-CoV-2 RNA-Dependent RNA Polymerase
Published 2022-01-01Subjects: Get full text
Article -
452
The RNA Polymerase Inhibitor Corallopyronin A Has a Lower Frequency of Resistance Than Rifampicin in <i>Staphylococcus aureus</i>
Published 2022-07-01“…Corallopyronin A (CorA) is active against Gram-positive bacteria and targets the switch region of RNA polymerase. Because of the high frequency of mutation (FoM) leading to rifampicin resistance, we determined the CorA FoM in <i>S. aureus</i> using fluctuation analysis at 4 × minimum inhibitory concentration (MIC). …”
Get full text
Article -
453
-
454
-
455
Towards the design of drugs against the dengue virus RNA dependent RNA polymerase : mechanistic studies of enzyme activities and substrate binding.
Published 2012“…Dengue NS5 consists of 2 domains- methyltransferase and RNA-dependent RNA polymerase (RdRp), interspersed by a 9 amino acid linker. …”
Get full text
Final Year Project (FYP) -
456
The transcription factor TFIIS zinc ribbon dipeptide Asp-Glu is critical for stimulation of elongation and RNA cleavage by RNA polymerase II
Published 2012“…The eukaryotic transcription factor TFIIS enhances elongation and nascent transcript cleavage activities of RNA polymerase II in a stalled elongation complex. By site-directed mutagenesis, we have demonstrated that invariant residues Asp-261 and Glu-262 of the nucleic acid-binding TFIIS Zn ribbon are critical for stimulation of both elongation and RNA cleavage activities of RNA polymerase II. …”
Get full text
Get full text
Conference Paper -
457
Binding to DNA of the RNA-polymerase II C-terminal domain allows discrimination between Cdk7 and Cdk9 phosphorylation
Published 2009“…The C-terminal domain (CTD) of RNA polymerase II regulates transcription through spatially and temporally coordinated events. …”
Journal article -
458
Gene silencing pathway RNA-dependent RNA polymerase of Neurospora crassa: yeast expression and crystallization of selenomethionated QDE-1 protein.
Published 2005“…The RNA-dependent RNA polymerase, QDE-1, is a component of the RNA silencing pathway in Neurospora crassa. …”
Journal article -
459
A single amino acid mutation in the PA subunit of the influenza virus RNA polymerase inhibits endonucleolytic cleavage of capped RNAs.
Published 2002“…The influenza A virus RNA-dependent RNA polymerase consists of three subunits-PB1, PB2, and PA. …”
Journal article -
460
Zn(2+) inhibits coronavirus and arterivirus RNA polymerase activity in vitro and zinc ionophores block the replication of these viruses in cell culture
Published 2010“…The RNA synthesis of these two distantly related nidoviruses is catalyzed by an RNA-dependent RNA polymerase (RdRp), which is the core enzyme of their multiprotein replication and transcription complex (RTC). …”
Journal article