The C-terminal domains of the PB2 subunit of the influenza A virus RNA polymerase directly interact with cellular GTPase Rab11a by Veler, H, Fan, H, Keown, JR, Sharps, J, Fournier, M, Grimes, JM, Fodor, E

“…Influenza A virus (IAV) contains a segmented RNA genome that is transcribed and replicated by the viral RNA polymerase in the cell nucleus. Replicated RNA segments are assembled with viral polymerase and oligomeric nucleoprotein into viral ribonucleoprotein (vRNP) complexes which are exported from the nucleus and transported across the cytoplasm to be packaged into progeny virions. …”

The transcription bubble of the RNA polymerase-promoter open complex exhibits conformational heterogeneity and millisecond-scale dynamics: Implications for transcription start-site selection by Robb, N, Cordes, T, Hwang, L, Gryte, K, Duchi, D, Craggs, T, Santoso, Y, Weiss, S, Ebright, R, Kapanidis, A

“…Bacterial transcription is initiated after RNA polymerase (RNAP) binds to promoter DNA, melts ~ 14 bp around the transcription start site and forms a single-stranded "transcription bubble" within a catalytically active RNAP-DNA open complex (RPo). …”

Multiplexed proteomics mapping of yeast RNA polymerase II and III allows near-complete sequence coverage and reveals several novel phosphorylation sites. by Mohammed, S, Lorenzen, K, Kerkhoven, R, van Breukelen, B, Vannini, A, Cramer, P, Heck, A

“…The multisubunit RNA polymerases (Pols) II and III synthesize mainly eukaryotic mRNAs and tRNAs, respectively. …”

The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain. by Vasiljeva, L, Kim, M, Mutschler, H, Buratowski, S, Meinhart, A

“…RNA polymerase II (Pol II) in Saccharomyces cerevisiae can terminate transcription via several pathways. …”

AtCyp59 is a multidomain cyclophilin from Arabidopsis thaliana that interacts with SR proteins and the C-terminal domain of the RNA polymerase II. by Gullerova, M, Barta, A, Lorkovic, Z

“…Indeed, by using yeast two-hybrid, in vitro pull-down, and immunoprecipitation assays, we found that AtCyp59 interacts with the C-terminal domain (CTD) of the largest subunit of RNA polymerase II. Ectopic expression of the tagged protein in Arabidopsis cell suspension resulted in highly reduced growth that is most probably due to reduced phosphorylation of the CTD. …”

The transcription bubble of the RNA polymerase-promoter open complex exhibits conformational heterogeneity and millisecond-scale dynamics: implications for transcription start-site selection. by Robb, N, Cordes, T, Hwang, L, Gryte, K, Duchi, D, Craggs, T, Santoso, Y, Weiss, S, Ebright, R, Kapanidis, A

“…Bacterial transcription is initiated after RNA polymerase (RNAP) binds to promoter DNA, melts ~14 bp around the transcription start site and forms a single-stranded "transcription bubble" within a catalytically active RNAP-DNA open complex (RP(o)). …”

The PB2 subunit of the influenza virus RNA polymerase affects virulence by interacting with the mitochondrial antiviral signaling protein and inhibiting expression of beta interferon. by Graef, K, Vreede, F, Lau, Y, McCall, A, Carr, S, Subbarao, K, Fodor, E

“…The PB2 subunit of the influenza virus RNA polymerase is a major virulence determinant of influenza viruses. …”

The plant organellar primase-helicase directs template recognition and primosome assembly via its zinc finger domain by Antolin Peralta-Castro, Francisco Cordoba-Andrade, Corina Díaz-Quezada, Rogerio Sotelo-Mundo, Robert Winkler, Luis G. Brieba

Subjects: “…RNA polymerase…”
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A simple method for generating high-resolution maps of genome-wide protein binding by Peter J Skene, Steven Henikoff

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Transcription initiation at a consensus bacterial promoter proceeds via a ‘bind-unwind-load-and-lock’ mechanism by Abhishek Mazumder, Richard H Ebright, Achillefs N Kapanidis

Subjects: “…RNA polymerase…”
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In vitro Transcription and Purification of RNAs of Different Size by Sofia Gallo, Maya Furler, Roland K.O. Sigel

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Template Entrance Channel as Possible Allosteric Inhibition and Resistance Site for Quinolines Tricyclic Derivatives in RNA Dependent RNA Polymerase of Bovine Viral Diarrhea Virus by Mitul Srivastava, Lovika Mittal, Debapriyo Sarmadhikari, Vijay Kumar Singh, Antonella Fais, Amit Kumar, Shailendra Asthana

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Multiplexed Genome Editing via an RNA Polymerase II Promoter-Driven sgRNA Array in the Diatom Phaeodactylum tricornutum: Insights Into the Role of StLDP by Yogesh Taparia, Achintya Kumar Dolui, Sammy Boussiba, Inna Khozin-Goldberg

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Interactions between the AraC/XylS-like transcriptional activator InvF of Salmonella Typhimurium, the RNA polymerase alpha subunit and the chaperone SicA by Daniel Cortés-Avalos, André Borges Farias, Luis E. Romero-González, Cristina Lara-Ochoa, Lourdes Villa-Tanaca, Francisco García-del Portillo, Vanessa López-Guerrero, Víctor H. Bustamante, Ernesto Pérez-Rueda, J. Antonio Ibarra

“…InvF very likely acts as a classical activator; however, whether InvF interacts with the RNA polymerase alpha subunit RpoA has not been determined. …”
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Riluzole partially restores RNA polymerase III complex assembly in cells expressing the leukodystrophy-causative variant POLR3B R103H by Maxime Pinard, Samaneh Dastpeyman, Christian Poitras, Geneviève Bernard, Marie-Soleil Gauthier, Benoit Coulombe

Subjects: “…RNA polymerase III…”
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The Phage-Encoded <i>N</i>-Acetyltransferase Rac Mediates Inactivation of <i>Pseudomonas aeruginosa</i> Transcription by Cleavage of the RNA Polymerase Alpha Subunit by Pieter-Jan Ceyssens, Jeroen De Smet, Jeroen Wagemans, Natalia Akulenko, Evgeny Klimuk, Subray Hedge, Marleen Voet, Hanne Hendrix, Jan Paeshuyse, Bart Landuyt, Hua Xu, John Blanchard, Konstantin Severinov, Rob Lavigne

“…These phages encode a single-subunit RNA polymerase for transcription of their late (structure- and lysis-associated) genes, whereas the bacterial RNA polymerase is used at the earlier stages of infection. …”
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RBM22 regulates RNA polymerase II 5′ pausing, elongation rate, and termination by coordinating 7SK-P-TEFb complex and SPT5 by Xian Du, Wenying Qin, Chunyu Yang, Lin Dai, Mingkui San, Yingdan Xia, Siyu Zhou, Mengyang Wang, Shuang Wu, Shaorui Zhang, Huiting Zhou, Fangshu Li, Fang He, Jingfeng Tang, Jia-Yu Chen, Yu Zhou, Rui Xiao

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Correction: TAL Effectors Target the C-Terminal Domain of RNA Polymerase II (CTD) by Inhibiting the Prolyl-Isomerase Activity of a CTD-Associated Cyclophilin

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Investigating Influenza Virus Polymerase Activity in Feline Cells Based on the Influenza Virus Minigenome Replication System Driven by the Feline RNA Polymerase I Promoter by Gang Lu, Feiyan Zheng, Jiajun Ou, Xin Yin, Shoujun Li, Shoujun Li, Shoujun Li

Subjects: “…RNA polymerase I promoter…”
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The Unusual Architecture of RNA-Dependent RNA Polymerase (RdRp)’s Catalytic Chamber Provides a Potential Strategy for Combination Therapy against COVID-19 by Kamel Metwally, Nader E. Abo-Dya, Mohammed Issa Alahmdi, Maha Z. Albalawi, Galal Yahya, Aimen Aljoundi, Elliasu Y. Salifu, Ghazi Elamin, Mahmoud A. A. Ibrahim, Yasien Sayed, Sylvia Fanucchi, Mahmoud E. S. Soliman

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