Drosophila Kismet regulates histone H3 lysine 27 methylation and early elongation by RNA polymerase II. by Shrividhya Srinivasan, Kristel M Dorighi, John W Tamkun

“…The trithorax group protein Kismet-L (KIS-L) is a member of the CHD subfamily of chromatin-remodeling factors that plays a global role in transcription by RNA polymerase II (Pol II). Mutations in CHD7, the human counterpart of kis, are associated with CHARGE syndrome, a developmental disorder affecting multiple tissues and organs. …”
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Tyrosine phosphorylation of RNA polymerase II CTD is associated with antisense promoter transcription and active enhancers in mammalian cells by Nicolas Descostes, Martin Heidemann, Lionel Spinelli, Roland Schüller, Muhammad Ahmad Maqbool, Romain Fenouil, Frederic Koch, Charlène Innocenti, Marta Gut, Ivo Gut, Dirk Eick, Jean-Christophe Andrau

Subjects: “…RNA polymerase II…”
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Distinct role of subunits of the Arabidopsis RNA polymerase II elongation factor PAF1C in transcriptional reprogramming by Simon Obermeyer, Richard Stöckl, Tobias Schnekenburger, Christoph Moehle, Uwe Schwartz, Klaus D. Grasser

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Coupling of PARP1-mediated chromatin structural changes to transcriptional RNA polymerase II elongation and cotranscriptional splicing by Elena A. Matveeva, Qamar M. H. Al-Tinawi, Eric C. Rouchka, Yvonne N. Fondufe-Mittendorf

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A-MYB and BRDT-dependent RNA Polymerase II pause release orchestrates transcriptional regulation in mammalian meiosis by Adriana K. Alexander, Edward J. Rice, Jelena Lujic, Leah E. Simon, Stephanie Tanis, Gilad Barshad, Lina Zhu, Jyoti Lama, Paula E. Cohen, Charles G. Danko

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Bridge helix bending promotes RNA polymerase II backtracking through a critical and conserved threonine residue by Lin-Tai Da, Fátima Pardo-Avila, Liang Xu, Daniel-Adriano Silva, Lu Zhang, Xin Gao, Dong Wang, Xuhui Huang

“…RNA Polymerase II can detect and cleave mis-incorporated nucleotides by a proofreading mechanism that requires backtracking of the enzyme. …”
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Complete dissection of transcription elongation reveals slow translocation of RNA polymerase II in a linear ratchet mechanism by Manchuta Dangkulwanich, Toyotaka Ishibashi, Shixin Liu, Maria L Kireeva, Lucyna Lubkowska, Mikhail Kashlev, Carlos J Bustamante

Subjects: “…RNA polymerase II…”
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Live-cell imaging reveals the spatiotemporal organization of endogenous RNA polymerase II phosphorylation at a single gene by Linda S. Forero-Quintero, William Raymond, Tetsuya Handa, Matthew N. Saxton, Tatsuya Morisaki, Hiroshi Kimura, Edouard Bertrand, Brian Munsky, Timothy J. Stasevich

“…During transcription, RNA polymerase II (RNAP2) is recruited to promoters and phosphorylated stepwise; so far, these steps have not been visualized in a single-copy gene in live cells. …”
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Slow nucleosome dynamics set the transcriptional speed limit and induce RNA polymerase II traffic jams and bursts. by Robert C Mines, Tomasz Lipniacki, Xiling Shen

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Exonuclease resistant 18S and 25S ribosomal RNA components in yeast are possibly newly transcribed by RNA polymerase II by Jacob Fleischmann, Miguel A. Rocha, Peter V. Hauser, Bhavani S. Gowda, Mary Grace D. Pilapil

“…If definitively proven it will establish an additional role for RNA Polymerase II in ribosomal production.…”
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GAGA factor maintains nucleosome-free regions and has a role in RNA polymerase II recruitment to promoters. by Nicholas J Fuda, Michael J Guertin, Sumeet Sharma, Charles G Danko, André L Martins, Adam Siepel, John T Lis

“…Previous studies have shown that GAGA Factor (GAF) is enriched on promoters with paused RNA Polymerase II (Pol II), but its genome-wide function and mechanism of action remain largely uncharacterized. …”
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Stimulation of transcript elongation requires both the zinc finger and RNA polymerase II binding domains of human TFIIS by Agarwal, Kan, Baek, Kwanghee, Jeon, Choon Ju, Miyamoto, Kenichi, Ueno, Akemichi, Yoon, Ho Sup

“…These findings suggest that TFIIS may interact with RNA polymerase II such that the normally masked zinc finger can specifically contact nucleotides in the transcription elongation zone at a position juxtaposed to the polymerization site.…”
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The 3' ends of human pre-snRNAs are produced by RNA polymerase II CTD-dependent RNA processing. by Uguen, P, Murphy, S

Poly(A) signals and transcriptional pause sites combine to prevent interference between RNA polymerase II promoters. by Eggermont, J, Proudfoot, N

“…Transcriptional termination by RNA polymerase II at the 3' end of genes encoding poly(A)+ mRNAs is thought to require two distinct cis-active elements: a functional poly(A) signal and a downstream transcriptional pause site. …”

Partial truncation of the yeast RNA polymerase II carboxyl-terminal domain preferentially reduces expression of glycolytic genes. by Meisels, E, Gileadi, O, Corden, J

“…The largest subunit of RNA polymerase II contains an essential carboxyl-terminal domain (CTD) that consists of highly conserved heptapeptide repeats with the consensus sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser. …”

Polycomb associates genome-wide with a specific RNA polymerase II variant, and regulates metabolic genes in ESCs. by Brookes, E, de Santiago, I, Hebenstreit, D, Morris, K, Carroll, T, Xie, S, Stock, J, Heidemann, M, Eick, D, Nozaki, N, Kimura, H, Ragoussis, J, Teichmann, SA, Pombo, A

“…Polycomb silencing in embryonic stem cells (ESCs) can be accompanied by active chromatin and primed RNA polymerase II (RNAPII), but the relationship between PRCs and RNAPII remains unclear genome-wide. …”

CDK9 and PP2A regulate RNA polymerase II transcription termination and coupled RNA maturation by Tellier, M, Zaborowska, J, Neve, J, Nojima, T, Hester, S, Fournier, M, Furger, A, Murphy, S

“…CDK9 is a kinase critical for the productive transcription of protein-coding genes by RNA polymerase II (pol II). As part of P-TEFb, CDK9 phosphorylates the carboxyl-terminal domain (CTD) of pol II and elongation factors, which allows pol II to elongate past the early elongation checkpoint (EEC) encountered soon after initiation. …”

Plasmodium falciparum var gene expression is developmentally controlled at the level of RNA polymerase II-mediated transcription initiation. by Kyes, S, Christodoulou, Z, Pinches, R, Kriek, N, Horrocks, P, Newbold, C

“…We demonstrate here that exclusive var gene expression is controlled at the level of transcription initiation during ring stages and that var genes are transcribed by RNA polymerase II. This represents another example where P. falciparum differs from the paradigm for antigenic variation, Trypanosoma brucei.…”

Translin facilitates RNA polymerase II dissociation and suppresses genome instability during RNase H2- and Dicer-deficiency. by Natalia Gomez-Escobar, Ahad A A Alsaiari, Hanadi A S Alahamadi, Othman Alzahrani, Ellen Vernon, Hussam A E Althagafi, Nasser S Almobadel, David W Pryce, Jane A Wakeman, Ramsay J McFarlane

“…Here we reveal a Trax- and RNAi-independent function for Translin in dissociating RNA polymerase II from its genomic template, with loss of Translin function resulting in increased transcription-associated recombination and elevated genome instability. …”
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PARP1′s Involvement in RNA Polymerase II Elongation: Pausing and Releasing Regulation through the Integrator and Super Elongation Complex by Elena A. Matveeva, Hejer Dhahri, Yvonne Fondufe-Mittendorf

“…Understanding the mechanism and factors critical in regulating the rate of RNA polymerase II elongation and processivity is clearly important. …”
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