αKG-driven RNA polymerase II transcription of cyclin D1 licenses malic enzyme 2 to promote cell-cycle progression by Yanting Yang, Zhenxi Zhang, Wei Li, Yufan Si, Li Li, Wenjing Du

“…Mechanistically, αKG directly binds to RNA polymerase II (RNAPII) and increases the level of RNAPII binding to the cyclin D1 gene promoter via promoting pre-initiation complex (PIC) assembly, consequently enhancing cyclin D1 transcription. …”
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Including Protons in Solid-State NMR Resonance Assignment and Secondary Structure Analysis: The Example of RNA Polymerase II Subunits Rpo4/7 by Anahit Torosyan, Thomas Wiegand, Maarten Schledorn, Daniel Klose, Peter Güntert, Peter Güntert, Peter Güntert, Anja Böckmann, Beat H. Meier

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Multiplexed proteomics mapping of yeast RNA polymerase II and III allows near-complete sequence coverage and reveals several novel phosphorylation sites. by Mohammed, S, Lorenzen, K, Kerkhoven, R, van Breukelen, B, Vannini, A, Cramer, P, Heck, A

The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain. by Vasiljeva, L, Kim, M, Mutschler, H, Buratowski, S, Meinhart, A

“…RNA polymerase II (Pol II) in Saccharomyces cerevisiae can terminate transcription via several pathways. …”

AtCyp59 is a multidomain cyclophilin from Arabidopsis thaliana that interacts with SR proteins and the C-terminal domain of the RNA polymerase II. by Gullerova, M, Barta, A, Lorkovic, Z

“…Indeed, by using yeast two-hybrid, in vitro pull-down, and immunoprecipitation assays, we found that AtCyp59 interacts with the C-terminal domain (CTD) of the largest subunit of RNA polymerase II. Ectopic expression of the tagged protein in Arabidopsis cell suspension resulted in highly reduced growth that is most probably due to reduced phosphorylation of the CTD. …”

Development of a T7-Independent MARV Minigenome System by Bert Vanmechelen, Joren Stroobants, Kurt Vermeire, Piet Maes

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Multiplexed Genome Editing via an RNA Polymerase II Promoter-Driven sgRNA Array in the Diatom Phaeodactylum tricornutum: Insights Into the Role of StLDP by Yogesh Taparia, Achintya Kumar Dolui, Sammy Boussiba, Inna Khozin-Goldberg

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Human cytomegalovirus IE2 drives transcription initiation from a select subset of late infection viral promoters by host RNA polymerase II. by Ming Li, Christopher B Ball, Geoffrey Collins, Qiaolin Hu, Donal S Luse, David H Price, Jeffery L Meier

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RBM22 regulates RNA polymerase II 5′ pausing, elongation rate, and termination by coordinating 7SK-P-TEFb complex and SPT5 by Xian Du, Wenying Qin, Chunyu Yang, Lin Dai, Mingkui San, Yingdan Xia, Siyu Zhou, Mengyang Wang, Shuang Wu, Shaorui Zhang, Huiting Zhou, Fangshu Li, Fang He, Jingfeng Tang, Jia-Yu Chen, Yu Zhou, Rui Xiao

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Correction: TAL Effectors Target the C-Terminal Domain of RNA Polymerase II (CTD) by Inhibiting the Prolyl-Isomerase Activity of a CTD-Associated Cyclophilin

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TFIIB is only ∼9 Å away from the 5'-end of a trimeric RNA primer in a functional RNA polymerase II preinitiation complex. by Matthew J Bick, Sohail Malik, Arkady Mustaev, Seth A Darst

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RNA Polymerase II Transcription Attenuation at the Yeast DNA Repair Gene, DEF1, Involves Sen1-Dependent and Polyadenylation Site-Dependent Termination by Courtney Whalen, Christine Tuohy, Thomas Tallo, James W. Kaufman, Claire Moore, Jason N. Kuehner

Subjects: “…RNA polymerase II termination…”
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Norovirus Replication in Human Intestinal Epithelial Cells Is Restricted by the Interferon-Induced JAK/STAT Signaling Pathway and RNA Polymerase II-Mediated Transcriptional Responses by Myra Hosmillo, Yasmin Chaudhry, Komal Nayak, Frederic Sorgeloos, Bon-Kyoung Koo, Alessandra Merenda, Reidun Lillestol, Lydia Drumright, Matthias Zilbauer, Ian Goodfellow

“…Surprisingly, targeted inhibition of cellular RNA polymerase II-mediated transcription was not detrimental to HuNoV replication but instead enhanced replication to a greater degree than blocking of JAK signaling directly. …”
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Human RNA polymerase II-association factor 1 (hPaf1/PD2) regulates histone methylation and chromatin remodeling in pancreatic cancer. by Parama Dey, Moorthy P Ponnusamy, Shonali Deb, Surinder K Batra

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AATF/Che-1 RNA polymerase II binding protein overexpression reduces the anti-tumor NK-cell cytotoxicity through activating receptors modulation by Matteo Caforio, Nicola Tumino, Cristina Sorino, Isabella Manni, Stefano Di Giovenale, Giulia Piaggio, Simona Iezzi, Georgios Strimpakos, Elisabetta Mattei, Lorenzo Moretta, M. Fanciulli, Paola Vacca, Franco Locatelli, Franco Locatelli, Valentina Folgiero

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Trichoderma reesei XYR1 activates cellulase gene expression via interaction with the Mediator subunit TrGAL11 to recruit RNA polymerase II. by Fanglin Zheng, Yanli Cao, Renfei Yang, Lei Wang, Xinxing Lv, Weixin Zhang, Xiangfeng Meng, Weifeng Liu

“…This differential involvement of TrGAL11 in the full induction of cellulase genes was reflected by the RNA polymerase II (Pol II) recruitment on their core promoters, indicating that TrGAL11 was required for the efficient transcriptional initiation of the majority of cellulase genes. …”
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Quantitation of RNA polymerase II and its transcription factors in an HeLa cell: little soluble holoenzyme but significant amounts of polymerases attached to the nuclear substructure. by Kimura, H, Tao, Y, Roeder, R, Cook, P

“…Various complexes that contain the core subunits of RNA polymerase II associated with different transcription factors have been isolated from eukaryotes; their precise molecular constitution depends on the purification procedure. …”

Nuclear IGF1R interacts with regulatory regions of chromatin to promote RNA Polymerase II recruitment and gene expression associated with advanced tumor stage by Aleksic, T, Gray, N, Wu, X, Rieunier, G, Osher, E, Mills, J, Verrill, C, Bryant, R, Han, C, Hutchinson, K, Lambert, A, Kumar, R, Hamdy, F, Weyer-Czernilofsky, U, Sanderson, M, Bogenrieder, T, Taylor, S, Macaulay, V

“…Using ChIP-seq to assess global chromatin occupancy, we identified IGF1R-binding sites at or near transcription start sites of genes including JUN and FAM21, most sites coinciding with occupancy by RNA polymerase II (RNAPol2) and histone marks of active enhancers/promoters. …”

RNA-free and ribonucleoprotein-associated influenza virus polymerases directly bind the serine-5 phosphorylated carboxyl-terminal domain of host RNA polymerase II by Fodor, E, Hengrung, N, Martinez-Alonso, M

“…Ongoing transcription by cellular RNA polymerase II (Pol II) is required for viral mRNA synthesis. …”

The F-Actin-Binding MPRIP Forms Phase-Separated Condensates and Associates with PI(4,5)P2 and Active RNA Polymerase II in the Cell Nucleus by Can Balaban, Martin Sztacho, Michaela Blažíková, Pavel Hozák

“…We identified MPRIP as a component of RNA Polymerase II/Nuclear Myosin 1 complex and showed that MPRIP forms phase-separated condensates which are able to bind nuclear F-actin fibers. …”
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