Antisense oligonucleotide therapeutics in clinical trials for the treatment of inherited retinal diseases by Xue, K, MacLaren, RE

“…They could modulate pre-messenger RNA splicing, induce mRNA knockdown, or block translation of disease-causing genes, thereby slowing disease progression. …”

The upstream sequence element of the C2 complement poly(A) signal activates mRNA 3' end formation by two distinct mechanisms. by Moreira, A, Takagaki, Y, Brackenridge, S, Wollerton, M, Manley, J, Proudfoot, N

“…We also show that the C2 USE can be cross-linked efficiently to a 55-kD protein that we identify as the polypyrimidine tract-binding protein (PTB), implicated previously in modulation of pre-mRNA splicing. Mutation of the PTB-binding site significantly reduces the efficiency of the C2 poly(A) site both in vivo and in vitro. …”

FBXW7 influences murine intestinal homeostasis and cancer, targeting Notch, Jun, and DEK for degradation. by Babaei-Jadidi, R, Li, N, Saadeddin, A, Spencer-Dene, B, Jandke, A, Muhammad, B, Ibrahim, E, Muraleedharan, R, Abuzinadah, M, Davis, H, Lewis, A, Watson, S, Behrens, A, Tomlinson, I, Nateri, A

“…DEK accumulation and altered TPM RNA splicing were also detected in FBXW7 mutant human colorectal tumor tissues. …”

Dual RNA sequencing of Helicobacter pylori and host cell transcriptomes reveals ontologically distinct host-pathogen interaction by Wei Hu, Zhi Yong Zhai, Zhao Yu Huang, Ze Min Chen, Ping Zhou, Xia Xi Li, Gen Hua Yang, Chong Ju Bao, Li Juan You, Xiao Bing Cui, Gui Li Xia, Mei Ping Ou Yang, Lin Zhang, William Ka Kei Wu, Long Fei Li, Yu Xuan Zhang, Zhan Gang Xiao, Wei Gong

“…Temporal profiling of host mRNA signatures dissected the aberrant pre-mRNA splicing of functional genes involved in the cell cycle process in response to H. pylori infection. …”
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A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma by Yue Li, Boyu Chen, Xingyu Jiang, Yudong Li, Xin Wang, Shumei Huang, Xuxia Wu, Yunyun Xiao, Dongni Shi, Xinjian Huang, Lixin He, Xiangfu Chen, Ying Ouyang, Jun Li, Libing Song, Chuyong Lin

“…These findings uncover the mechanism for Wnt signaling in lncRNA splicing and suggest that the DGCR5 splicing switch may be a targetable vulnerability in ESCC.…”
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PTBP2-Mediated Alternative Splicing of IRF9 Controls Tumor-Associated Monocyte/Macrophage Chemotaxis and Repolarization in Neuroblastoma Progression by Jue Tang, Jing He, Huiqin Guo, Huiran Lin, Meng Li, Tianyou Yang, Hai-Yun Wang, Di Li, Jiabin Liu, Le Li, Huimin Xia, Zhenjian Zhuo, Lei Miao

“…Our study defined a critical event of PTBP2-induced monocytes/Mϕs in NB progression and revealed that RNA splicing occurred by PTBP2 benefits immune compartmentalization between NB cells and monocytes. …”
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Targeting the RNA-Binding Protein <i>QKI</i> in Myeloid Cells Ameliorates Macrophage-Induced Renal Interstitial Fibrosis by Ruben G. de Bruin, Gillian Vogel, Jurrien Prins, Jacques M. J. G. Duijs, Roel Bijkerk, Hendrik J. P. van der Zande, Janine M. van Gils, Hetty C. de Boer, Ton J. Rabelink, Anton Jan van Zonneveld, Eric P. van der Veer, Stéphane Richard

“…We show that macrophages isolated from <i>QKI</i>^{FL/FL;LysM-Cre} mice are associated with defects in pre-mRNA splicing. Our findings demonstrate that reduced expression of the alternative splice regulator QKI in the cells of myeloid lineage attenuates renal interstitial fibrosis, suggesting that inhibition of this splice regulator may be of therapeutic value for certain kidney diseases.…”
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Modulation of 5' splice site selection using tailed oligonucleotides carrying splicing signals by Elela Sherif, Klinck Roscoe, Villemaire Jonathan, Garneau Daniel, Carriero Sandra, Gendron Daniel, Damha Masad J, Chabot Benoit

“…<p>Abstract</p> <p>Background</p> <p>We previously described the use of tailed oligonucleotides as a means of reprogramming alternative pre-mRNA splicing in vitro and in vivo. The tailed oligonucleotides that were used interfere with splicing because they contain a portion complementary to sequences immediately upstream of the target 5' splice site combined with a non-hybridizing 5' tail carrying binding sites for the hnRNP A1/A2 proteins.…”
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RBM24 in the Post-Transcriptional Regulation of Cancer Progression: Anti-Tumor or Pro-Tumor Activity? by De-Li Shi

“…RBM24 is a highly conserved protein that binds to a large spectrum of target mRNAs and regulates many post-transcriptional events ranging from pre-mRNA splicing to mRNA stability, polyadenylation and translation. …”
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Experimental Confirmation of a Whole Set of tRNA Molecules in Two Archaeal Species by Yoh-ichi Watanabe, Yutaka Kawarabayasi

“…Whole-set analyses of tRNA molecules revealed that the archaeal tRNA splicing mechanism may be essential for efficient splicing of all tRNAs produced from interrupted tRNA genes in these archaea.…”
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Alternative Splicing Role in New Therapies of Spinal Muscular Atrophy by Jan Lejman, Grzegorz Zieliński, Piotr Gawda, Monika Lejman

“…Understanding the regulation of the <i>SMN2</i> pre-mRNA splicing process has allowed for innovative treatment and the introduction of new medicines for SMA. …”
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Deregulated microRNAs Involved in Prostate Cancer Aggressiveness and Treatment Resistance Mechanisms by Himali Gujrati, Siyoung Ha, Bi-Dar Wang

“…In particular, the oncogenic and tumor-suppressive miRNAs involved in the regulation of (1) the synthesis of the androgen receptor (AR) and its AR-V7 splice variant, (2) PTEN expression and PTEN-mediated signaling, (3) RNA splicing mechanisms, (4) chemo- and hormone-therapy resistance, and (5) racial disparities in PCa are discussed and summarized. …”
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ADP-Ribosylation Post-Translational Modification: An Overview with a Focus on RNA Biology and New Pharmacological Perspectives by Giuseppe Manco, Giuseppina Lacerra, Elena Porzio, Giuliana Catara

“…Cellular functions are regulated through the gene expression program by the transcription of new messenger RNAs (mRNAs), alternative RNA splicing, and protein synthesis. To this end, the post-translational modifications (PTMs) of proteins add another layer of complexity, creating a continuously fine-tuned regulatory network. …”
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Somatic gene mutations expose cytoplasmic DNA to co-opt the cGAS/STING/NLRP3 axis in myelodysplastic syndromes by Amy F. McLemore, Hsin-An Hou, Benjamin S. Meyer, Nghi B. Lam, Grace A. Ward, Amy L. Aldrich, Matthew A. Rodrigues, Alexis Vedder, Ling Zhang, Eric Padron, Nicole D. Vincelette, David A. Sallman, Omar Abdel-Wahab, Alan F. List, Kathy L. McGraw

“…Gene mutations involving mRNA splicing and epigenetic regulatory pathways induce inflammasome activation and myeloid lineage skewing in MDSs through undefined mechanisms. …”
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RPflex: A Coarse-Grained Network Model for RNA Pocket Flexibility Study by Chen Zhuo, Chengwei Zeng, Rui Yang, Haoquan Liu, Yunjie Zhao

“…RNA regulates various biological processes, such as gene regulation, RNA splicing, and intracellular signal transduction. …”
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Profiling and Validation of the Circular RNA Repertoire in Adult Murine Hearts by Tobias Jakobi, Lisa F. Czaja-Hasse, Richard Reinhardt, Christoph Dieterich

“…Several studies have related RNA splicing to cardiovascular disease and circular RNAs (circRNAs) are an emerging player. circRNAs, which originate through back-splicing events from primary transcripts, are resistant to exonucleases and typically not polyadenylated. …”
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The Role of PRMT5 in Immuno-Oncology by Yoshinori Abe, Takumi Sano, Nobuyuki Tanaka

“…Protein arginine methyltransferase 5 (PRMT5) di-methylates arginine residues in its substrates and has essential roles in the epigenetic regulation of gene expression, signal transduction, and the fidelity of mRNA splicing. Through these functions, PRMT5 can support cancer cell immune evasion. …”
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A Genome-Wide Screen for the Exonisation of Reference SINE-VNTR-Alus and Their Expression in CNS Tissues of Individuals with Amyotrophic Lateral Sclerosis by Abigail L. Pfaff, Vivien J. Bubb, John P. Quinn, Sulev Koks

“…This study demonstrates the functional consequences of several SVA elements altering mRNA splicing patterns and expression levels in tissues of the CNS.…”
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Non-Coding RNAs in the Regulation of Hippocampal Neurogenesis and Potential Treatment Targets for Related Disorders by Zhengye Tan, Wen Li, Xiang Cheng, Qing Zhu, Xinhua Zhang

“…Nonetheless, they have critical roles in a variety of cellular activities—such as development, neurogenesis, degeneration, and the response to injury to the nervous system—via protein translation, RNA splicing, gene activation, silencing, modifications, and editing; thus, they may serve as potential targets for disease treatment. …”
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The translational landscape of the splicing factor SRSF1 and its role in mitosis by Magdalena M Maslon, Sara R Heras, Nicolas Bellora, Eduardo Eyras, Javier F Cáceres

“…Furthermore, we found that mRNAs that display alternative splicing changes upon SRSF1 overexpression are also its translational targets, strongly suggesting that SRSF1 couples pre-mRNA splicing and translation. These data provide insights on the complex role of SRSF1 in the control of gene expression at multiple levels and its implications in cancer.…”
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