Single-cell long-read sequencing in human cerebral organoids uncovers cell-type-specific and autism-associated exons by Yalan Yang, Runwei Yang, Bowei Kang, Sheng Qian, Xin He, Xiaochang Zhang

“…Retained neuronal introns are enriched in RNA splicing regulators, showing shorter lengths, higher GC contents, and weaker 5′ splice sites. …”
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Non-canonical functions of spliceosome components in cancer progression by Olga M. Ivanova, Ksenia S. Anufrieva, Anastasia N. Kazakova, Irina K. Malyants, Polina V. Shnaider, Maria M. Lukina, Victoria O. Shender

“…Abstract Dysregulation of pre-mRNA splicing is a common hallmark of cancer cells and it is associated with altered expression, localization, and mutations of the components of the splicing machinery. …”
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Mutual regulation between N6-methyladenosine (m6A) modification and circular RNAs in cancer: impacts on therapeutic resistance by Hong Lin, Yuxi Wang, Pinghan Wang, Fangyi Long, Ting Wang

“…N6-methyladenosine (m6A) is the most prevalent RNA modification in eukaryotes, and is involved in the regulation of RNA splicing, translation, transport, degradation, stability and processing, thus affecting several physiological processes and cancer progression. …”
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SMC4, a novel tumor prognostic marker and potential tumor therapeutic target by Zonglei Zhao, Xixiu Wang, Yan Ding, Xuefeng Cao, Xingyuan Zhang

“…Studies have also shown that SMC4 plays an exceedingly essential role in the division cycle of embryonic cells, such as RNA splicing, DNA metabolic process, cell adhesion, and extracellular matrix. …”
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Identification of Potential Biomarkers and Small Molecule Drugs for Bisphosphonate-Related Osteonecrosis of the Jaw (BRONJ): An Integrated Bioinformatics Study Using Big Data by Kumarendran Balachandran, Roszalina Ramli, Saiful Anuar Karsani, Mariati Abdul Rahman

“…A total of 1481 differentially expressed genes were identified, including 381 upregulated and 1100 downregulated genes, with enriched functions and pathways related to apoptosis, RNA splicing, signaling pathways, and lipid metabolism. …”
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Psoriasis-Specific RNA Isoforms Identified by RNA-seq Analysis of 173,446 Transcripts by Sulev Kõks, Maris Keermann, Ene Reimann, Ele Prans, Kristi Abram, Helgi Silm, Gea Koks, Kulli Kingo

“…Methods: Using our published RNA-sequencing data from psoriatic lesional (LP), psoriatic non-lesional (NLP) and normal control skin (C), we analysed the differential expression of RNA splicing variants. LP sample was compared with NLP, as was LP with C and NLP with C. …”
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Connecting RNA-Modifying Similarities of TDP-43, FUS, and SOD1 with MicroRNA Dysregulation Amidst A Renewed Network Perspective of Amyotrophic Lateral Sclerosis Proteinopathy by Jade Pham, Matt Keon, Samuel Brennan, Nitin Saksena

“…Moreover, the cytoplasmic mislocalization, aggregation, and co-aggregation of TDP-43, FUS, and SOD1 can be identified as proteinopathies akin to other neurodegenerative diseases (NDs), eliciting strong ties to disrupted RNA splicing, transport, and stability. In recent years, microRNAs (miRNAs) have also been increasingly implicated in the disease, and are of greater significance as they are the master regulators of RNA metabolism in disease pathology. …”
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Sex differences in gene expression and alternative splicing in the Chinese horseshoe bat by Wenli Chen, Weiwei Zhou, Qianqian Li, Xiuguang Mao

“…In addition, we also detected many differentially spliced genes between the sexes (DSGs, 2,231 and 1,027 in the brain and liver, respectively) which were mainly involved in regulation of RNA splicing and mRNA metabolic process. Interestingly, we found a significant enrichment of DEGs on the X chromosome, but not for DSGs. …”
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The Posttranscriptional Mechanism in <i>Salvia miltiorrhiza</i> Bunge Leaves in Response to Drought Stress Using Phosphoproteomics by Jin Zhang, Jingyu Li, Yuekai Su, Zhenqiao Song, Jianhua Wang

“…Additionally, we identified that the Ser phosphorylation levels of most proteins related to terpene metabolism and RNA splicing were regulated by drought stresses. The Ser and Thr phosphorylation levels of energy metabolism proteins (including FBA2/8, PPC4, and PPCC) and heat shock proteins (including HSP70 and HSP90) were upregulated by drought stresses. …”
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Pericentric major satellite transcription is essential for meiotic chromosome stability and spindle pole organization by Claudia Baumann, Xiangyu Zhang, Maria M. Viveiros, Rabindranath De La Fuente

“…Inhibition of transcription or RNA splicing phenocopies the chromosome alignment errors and spindle defects, suggesting that pericentric transcription during oocyte meiosis is required to regulate heterochromatin structure, chromosome segregation and maintenance of spindle organization.…”
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Translational Regulation by hnRNP H/F Is Essential for the Proliferation and Survival of Glioblastoma by Morgane Le Bras, Noah Gorelick, Sylvain Pautet, Betty Tyler, Stéphane Manenti, Nicolas Skuli, Stefania Millevoi, Anne Cammas

“…Among these, hnRNP H/F regulate the phosphorylation of eIF4E and its translational targets by controlling RNA splicing of the <i>A-Raf</i> kinase mRNA, which in turn modulates the MEK-ERK/MAPK signaling pathway. …”
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Inducible de novo expression of neoantigens in tumor cells and mice by Damo, Martina, Fitzgerald, Brittany, Lu, Yisi, Nader, Mursal, William, Ivana, Cheung, Julie F., Connolly, Kelli A., Foster, Gena G., Akama-Garren, Elliot, Lee, Da-Yae, Chang, Greg P., Gocheva, Vasilena, Schmidt, Leah M., Boileve, Alice, Wilson, Josephine H., Cui, Can, Monroy, Isabel, Gokare, Prashanth, Cabeceiras, Peter, Jacks, Tyler, Joshi, Nikhil S.

“…Here we develop inversion-induced joined neoantigen (NINJA), using RNA splicing, DNA recombination and three levels of regulation to prevent leakiness and allow tight control over neoantigen expression. …”
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Analysis and design of RNA sequencing experiments for identifying isoform regulation by Katz, Yarden, Airoldi, Edoardo M., Wang, Eric T, Burge, Christopher B

“…Application of MISO implicated the RNA splicing factor hnRNP H1 in the regulation of alternative cleavage and polyadenylation, a role that was supported by UV cross-linking–immunoprecipitation sequencing (CLIP-seq) analysis in human cells. …”
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Deterministic Restriction on Pluripotent State Dissolution by Cell-Cycle Pathways by Gonzales, Kevin Andrew Uy, Liang, Hongqing, Lim, Yee-Siang, Chan, Yun-Shen, Yeo, Jia-Chi, Tan, Cheng-Peow, Gao, Bin, Le, Beilin, Tan, Zi-Ying, Low, Kok-Yao, Liou, Yih-Cherng, Bard, Frederic, Ng, Huck-Hui

“…In a high-throughput RNAi screen using an inclusive set of differentiation conditions, we identify centrally important and context-dependent processes regulating PSD in hESCs, including histone acetylation, chromatin remodeling, RNA splicing, and signaling pathways. Strikingly, we detected a strong and specific enrichment of cell-cycle genes involved in DNA replication and G2 phase progression. …”
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Recognition of atypical 5' splice sites by shifted base-pairing to U1 snRNA by Krainer, Adrian R., Roca, Xavier

“…Accurate pre-mRNA splicing is critical for gene expression. The 5' splice site (5' ss) — the highly diverse element at the 5' end of introns — is initially recognized via base-pairing to the 5' end of U1 small nuclear RNA (snRNA). …”
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Functional studies of BCL11A: characterization of the conserved BCL11A-XL splice variant and its interaction with BCL6 in nuclear paraspeckles of germinal center B cells. by Liu, H, Ippolito, G, Wall, J, Niu, T, Probst, L, Lee, B, Pulford, K, Banham, A, Stockwin, L, Shaffer, A, Staudt, L, Das, C, Dyer, M, Tucker, P

“…RESULTS: Alternative pre-mRNA splicing of BCL11A produces multiple isoforms sharing a common N-terminus. …”

Amyotrophic lateral sclerosis: Cell vulnerability or system vulnerability? by Talbot, K

“…Data implicating pathways including protein misfolding, mRNA splicing, oxidative stress, proteosome and mitochondrial dysfunction in the pathogenesis of ALS reinforce a disease model based on selective age-dependent vulnerability of a specific population of cells. …”

Genome-wide identification of alternative splice forms down-regulated by nonsense-mediated mRNA decay in Drosophila by Hansen, K, Lareau, L, Blanchette, M, Green, R, Meng, Q, Rehwinkel, J, Gallusser, F, Izaurralde, E, Rio, D, Dudoit, S, Brenner, SE

“…Alternative mRNA splicing adds a layer of regulation to the expression of thousands of genes in Drosophila melanogaster. …”

Analysis of Jmjd6 cellular localization and testing for its involvement in histone demethylation. by Hahn, P, Wegener, I, Burrells, A, Böse, J, Wolf, A, Erck, C, Butler, D, Schofield, C, Böttger, A, Lengeling, A

“…Jmjd6 has recently been shown to hydroxylate RNA splicing factors and is known to be essential for the differentiation of multiple tissues and cells during embryogenesis. …”

The N6-methyladenosine demethylase ALKBH5 regulates the hypoxic HBV transcriptome by Tsukuda, S, Harris, JM, Magri, A, Balfe, P, Siddiqui, A, Wing, PAC, McKeating, JA

“…Mapping the transcription start sites of BCP-RNAs identified a role for hypoxia to regulate pre-genomic RNA splicing that is dependent on m6A modification. Bioinformatic analysis of published single cell RNA-seq of murine liver showed an increased expression of the RNA demethylase ALKBH5 in the peri-central low oxygen region. …”