Set2‐mediated H3K36 methylation states redundantly repress the production of antisense transcripts: role in transcription regulation by Yu‐Chao Mei, Jiangpeng Feng, Fei He, Yu‐Min Li, Yafei Liu, Feng Li, Yu Chen, Hai‐Ning Du

“…The methylation of H3K36 is known to recruit different chromatin effector proteins, affecting transcription, mRNA splicing and DNA repair. In this study, we engineered two yeast set2 mutants that lack H3K36 mono/dimethylation (H3K36me1/2) and trimethylation (H3K36me3), respectively, and characterized their roles in the production of antisense transcripts under nutrient‐rich conditions. …”
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Regulation of age-dependent expression patterns of five transcription factors in Larix kaempferi by Xiangyi Li, Dongxia Cheng, Liwang Qi, Jinwei Zhan, Wanfeng Li

“…These results clearly show that both pre-mRNA transcription and splicing of five age-related genes are regulated by age, indicating that age-dependent expression patterns are controlled at both transcriptional and post-transcriptional levels, and unveiling the underlying regulatory molecular mechanisms should focus on the transcription factors, epigenetic regulation, and RNA splicing. These data provide new insights into the age-mediated regulation of gene expression in woody perennials in terms of longevity.…”
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Molecular Modelling of NONO and SFPQ Dimerization Process and RNA Recognition Mechanism by Tommaso Laurenzi, Luca Palazzolo, Elisa Taiana, Simona Saporiti, Omar Ben Mariem, Uliano Guerrini, Antonino Neri, Ivano Eberini

“…NONO and SFPQ are involved in multiple nuclear processes (e.g., pre-mRNA splicing, DNA repair, and transcriptional regulation). …”
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Transcriptomics of Hirschsprung disease patient-derived enteric neural crest cells reveals a role for oxidative phosphorylation by Zhixin Li, Kathy Nga-Chu Lui, Sin-Ting Lau, Frank Pui-Ling Lai, Peng Li, Patrick Ho-Yu Chung, Kenneth Kak-Yuen Wong, Paul Kwong-Hing Tam, Maria-Mercedes Garica-Barcelo, Chi-Chung Hui, Pak Chung Sham, Elly Sau-Wai Ngan

“…Furthermore, upregulation of RNA splicing mediators and enhanced alternative splicing events are associated with severe form of Hirschsprung. …”
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NFAT gene polymorphism as a risk factor of knee osteoarthrosis by V.B. Novakov, O.N. Novakova, M.I. Churnosov

“…The rs6499244 locus of NFAT5 gene is located in an area of DNAse I hypersensitivity; it increases DNA affinity to four transcription factors (CDP_6, RFX5_known1, RORalpha1_2, TCF4_known1); it is localized in functionally active promoters and enhancers; it is associated with expression of nine genes (CLEC18A, COG4, EXOSC6, NFAT5, NOB1, NPIPB14P, NQO1, PDXDC2P, SMG1P7) and alternative mRNA splicing of three genes (NOB1, NPIPB14P, NQO1) in various organs and tissues in the body including those that are pathogenetically significant for OA (fat tissue, tibial nerves and arteries, and skeletal muscles).…”
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Interrelationship between miRNA and splicing factors in pancreatic ductal adenocarcinoma by I Gede Putu Supadmanaba, Giulia Mantini, Ornella Randazzo, Mjriam Capula, Ittai B. Muller, Stella Cascioferro, Patrizia Diana, Godefridus J. Peters, Elisa Giovannetti

“…Deregulation of microRNA (miRNA) dependent gene expression and mRNA splicing are epigenetic processes that modulate the protein repertoire at the transcriptional level. …”
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Expression Profiles of Circulating MicroRNAs in XELOX-Chemotherapy-Induced Peripheral Neuropathy in Patients with Advanced Gastric Cancer by Yeongdon Ju, Young Mi Seol, Jungho Kim, Hyunwoo Jin, Go-Eun Choi, Aelee Jang

“…The identified genes are related to the peptidyl-serine phosphorylation and regulation of alternative mRNA splicing with enrichment in the gastric cancer, neurotrophin, MAPK, and AMPK signaling pathways. …”
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Genomic Breakpoint Characterization and Transcriptome Analysis of Metastatic, Recurrent Desmoplastic Small Round Cell Tumor by Justin W. Magrath, Dane A. Flinchum, Alifiani B. Hartono, Ilon N. Goldberg, Madelyn Espinosa-Cotton, Krzysztof Moroz, Nai-Kong V. Cheung, Sean B. Lee

“…Upregulated pathways in the recurrent tumor included several DNA repair and mRNA splicing-related pathways, while downregulated pathways included immune system function and focal adhesion. …”
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Reversal of splicing infidelity is a pre-activation step in B cell differentiation by Tina M. O’Grady, Melody Baddoo, Samuel A. Flemington, Eman Y. Ishaq, Nathan A. Ungerleider, Erik K. Flemington

“…Affected genes were enriched in functionally important immune cell activation pathways such as antigen-mediated signaling, cell cycle control and mRNA processing and splicing.DiscussionFunctional observations from donor B cell subsets in progressive states of differentiation and from timecourse experiments using the in vitro model suggest that these widespread changes in mRNA splicing play a role in preparing naïve B cells for the decisive step of antigen-mediated activation and differentiation.…”
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Multimodal imaging and genetic characteristics of Chinese patients with USH2A‐associated nonsyndromic retinitis pigmentosa by Chong Chen, Qiao Sun, Mingmin Gu, Tianwei Qian, Dawei Luo, Kun Liu, Xun Xu, Suqin Yu

“…Of the de novo mutations, the p.C931Y and p.G4489S mutations were predicted to be deleterious or probably damaging; the p.M4853V mutation was predicted to be neutral or benign; and the IVS22+3A>G mutation was a splicing mutation that could influence mRNA splicing and affect the formation of the hairpin structure of the USH2A protein. …”
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Elucidating the Role of HIV-2 Viral Protein X by Mohamed Mahdi, Tamás Richárd Linkner, Zsófia Ilona Szojka, József Tőzsér

“…Of particular interest was the interaction between HIV-2 Vpx and the pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15, which is required for HIV-1 viral DNA synthesis, and the eukaryotic translation initiation factor 2 subunit 3 (EIF2S3), involved in the early steps of protein synthesis. …”
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Comprehensive Identification and Expression Analysis of the YTH Family of RNA-Binding Proteins in Strawberry by Pengbo Xu, Xinyu Li, Junmiao Fan, Chong Wang, Anqi Lin, Hongli Lian

“…Plant growth and development processes are tightly regulated at multiple levels, including transcriptional and post-transcriptional levels, and the RNA-binding protein YTH regulates gene expression during growth and development at the post-transcriptional level by regulating RNA splicing, processing, stability, and translation. …”
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Differential susceptibility of male and female germ cells to glucocorticoid-mediated signaling by Steven A Cincotta, Nainoa Richardson, Mariko H Foecke, Diana J Laird

“…In contrast, our studies revealed that the male germline is susceptible to glucocorticoid-mediated signaling, specifically by regulating RNA splicing within the spermatogonia, although this does not abrogate fertility. …”
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Modelling the developmental spliceosomal craniofacial disorder Burn-McKeown syndrome using induced pluripotent stem cells. by Katherine A Wood, Charlie F Rowlands, Huw B Thomas, Steven Woods, Julieta O'Flaherty, Sofia Douzgou, Susan J Kimber, William G Newman, Raymond T O'Keefe

“…The craniofacial developmental disorder Burn-McKeown Syndrome (BMKS) is caused by biallelic variants in the pre-messenger RNA splicing factor gene TXNL4A/DIB1. The majority of affected individuals with BMKS have a 34 base pair deletion in the promoter region of one allele of TXNL4A combined with a loss-of-function variant on the other allele, resulting in reduced TXNL4A expression. …”
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CYP2C19 genetic polymorphism in the Vietnamese population by Nhung Phuong Vu, Hoa Thi Thanh Nguyen, Ngoc Thi Bich Tran, Ton Dang Nguyen, Hue Thi Thu Huynh, Xuan Thi Nguyen, Duong Thuy Nguyen, Hai Van Nong, Ha Hai Nguyen

“…Three novel variants in introns 2, 5 and 8 had no impact on mRNA splicing according to the Human Splicing Finder. …”
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Curriculum vitae of CUG binding protein 1 (CELF1) in homeostasis and diseases: a systematic review by Wan‑Jia Qin, Jin-Jin Shi, Ru-Yi Chen, Chang-Yun Li, Yan-Jun Liu, Jian-Fei Lu, Guan-Jun Yang, Jia-Feng Cao, Jiong Chen

“…CUG-BP, Elav-like family 1 (CELF1) is a member of the CELF family of RBPs with high affinity to the GU-rich elements in mRNA, and thus exerting control over critical processes including mRNA splicing, translation, and decay. Mounting studies support that CELF1 is correlated with occurrence, genesis and development and represents a potential therapeutical target for these malignant diseases. …”
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Transcriptome Sequencing Reveals Pathways Related to Proliferation and Differentiation of Shitou Goose Myoblasts by Jiahui Chen, Shuai Zhang, Genghua Chen, Xianqi Deng, Danlu Zhang, Huaqiang Wen, Yunqian Yin, Zetong Lin, Xiquan Zhang, Wen Luo

“…A total of 1664 differentially expressed (DE) mRNAs and 244 DE-lncRNAs were identified. The alternative mRNA splicing in proliferation and differentiation stages was also analyzed. …”
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Development of Engineered-U1 snRNA Therapies: Current Status by Mariana Gonçalves, Juliana Inês Santos, Maria Francisca Coutinho, Liliana Matos, Sandra Alves

“…The majority of human genes that encode proteins undergo alternative pre-mRNA splicing and mutations that affect splicing are more prevalent than previously thought. …”
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Yeast Lsm Pro-Apoptotic Mutants Show Defects in Autophagy by Benedetta Caraba, Mariarita Stirpe, Vanessa Palermo, Ugo Vaccher, Michele Maria Bianchi, Claudio Falcone, Cristina Mazzoni

“…<i>LSM4</i> is an essential yeast gene encoding a component of different LSM complexes involved in the regulation of mRNA splicing, stability, and translation. In previous papers, we reported that the expression in <i>S. cerevisiae</i> of the <i>K. lactis LSM4</i> gene lacking the C-terminal Q/N-rich domain in an <i>Lsm4</i> null strain <i>S. cerevisiae</i> (<i>Sclsm4Δ1</i>) restored cell viability. …”
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Exploring Pharmacological Functions of Alternatively Spliced Variants of the Mu Opioid Receptor Gene, <i>Oprm1</i>, via Gene-Targeted Animal Models by Wenjian Kang, Shan Liu, Jin Xu, Anna Abrimian, Ayma F. Malik, Raymond Chien, Adejuyigbe Adaralegbe, Akwasi Amponsah, Luca Cartegni, John Pintar, Ying-Xian Pan

“…The single-copy mu opioid receptor gene, <i>OPRM1</i>, goes through extensive alternative pre-mRNA splicing to generate numerous splice variants that are conserved from rodents to humans. …”
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