Novel XBP1s-independent function of IRE1 RNase in HIF-1α-mediated glycolysis upregulation in human macrophages upon stimulation with LPS or saturated fatty acid by Margaud Iovino, Megan Colonval, Chloé Wilkin, Laurent L’homme, Cédric Lassence, Manon Campas, Olivier Peulen, Pascal de Tullio, Jacques Piette, Sylvie Legrand-Poels

“…SFAs and LPS both induced IRE1α endoribonuclease activity, as demonstrated by XBP1 mRNA splicing, but with different kinetics matching HIF-1α activation and the glycolytic gene expression. …”
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PPM1G promotes cell proliferation via modulating mutant GOF p53 protein expression in hepatocellular carcinoma by Wen Hu, Shao-Lin Ma, Liang Qiong, Yu Du, Li-Ping Gong, Yu-Hang Pan, Li-Ping Sun, Jing-Yun Wen, Jian-Ning Chen, Xin-Yuan Guan, Chun-Kui Shao

“…Summary: The serine/threonine protein phosphatase family involves series of cellular processes, such as pre-mRNA splicing. The function of one of its members, protein phosphatase, Mg2+/Mn2+ dependent 1G (PPM1G), remains unclear in hepatocellular carcinoma (HCC). …”
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Transcriptomic Analysis Reveals mRNA and Alternative Splicing Events in Ovine Skeletal Muscle Satellite Cells during Proliferation and Differentiation by Qian Chen, Chang Huang, Yinxiao Su, Qian Zhao, Yabin Pu, Xiaohong He, Lin Jiang, Yuehui Ma, Qianjun Zhao, Shaohui Ye

“…Among them, a unique AS event was the major per-mRNA splicing type, and SE was the predominant splicing pattern. …”
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Genome-wide comparison and in silico analysis of splicing factor SYF2/NTC31/p29 in eukaryotes: Special focus on vertebrates by Bao-Xing Huang, Zi-Chang Jia, Zi-Chang Jia, Xue Yang, Chao-Lin Cheng, Xiao-Rong Liu, Jianhua Zhang, Mo-Xian Chen, Jing-Fang Yang, Yun-Sheng Chen

“…The gene SYF2—an RNA splicing factor—can interact with Cyclin D-type binding protein 1 (GICP) in many biological processes, including splicing regulation, cell cycle regulation, and DNA damage repair. …”
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An antisense Alu transposon insertion/deletion polymorphism of ALDH1A1 may functionally associate with Parkinson’s disease by Hui-Hui Fan, Jing Zheng, Xiao-Ya Huang, Ke-Yun Wu, Lei Cui, Hao-Jia Dong, Zhen Wang, Xiong Zhang, Jian-Hong Zhu

“…Mechanistic explorations suggested that the asYb8c4ins induced no changes in CpG methylation and mRNA splicing of ALDH1A1 and appeared no binding of transcription factors. …”
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Splicing dysregulation contributes to the pathogenicity of several F9 exonic point variants by Upendra K. Katneni, Aaron Liss, David Holcomb, Nobuko H. Katagiri, Ryan Hunt, Haim Bar, Amra Ismail, Anton A. Komar, Chava Kimchi‐Sarfaty

“…Abstract Background Pre‐mRNA splicing is a complex process requiring the identification of donor site, acceptor site, and branch point site with an adjacent polypyrimidine tract sequence. …”
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Pan-cancer analysis of the prognostic and immunological roles of DEAD-box helicase 5 (DDX5) in human tumors by Shixuan Liu, Shixuan Liu, Yanbin Liu, Xi Zhang, Xuanlin Song, Boxiang Zhang, Yong Zhang

“…Enrichment analysis suggested that DDX5 was associated with multiple cellular pathways, including RNA splicing, Notch signaling pathway, and viral carcinogenesis, which was consistent with the results of previous studies.Conclusion: The findings obtained herein provide further information on the oncogenic potential of DDX5 in diverse tumor types. …”
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<i>Downregulation of BUD31</i> Promotes Prostate Cancer Cell Proliferation and Migration via Activation of p-AKT and Vimentin In Vitro by Muhammad Choudhry, Yaser Gamallat, Ealia Khosh Kish, Sima Seyedi, Geoffrey Gotto, Sunita Ghosh, Tarek A. Bismar

“…BUD31, also known as Functional Spliceosome-Associated Protein 17, is a protein that works at the level of the spliceosome; it is functionally implicated in pre-mRNA splicing as well as processing, while also acting as a transcriptional regulator of androgen receptor (AR) target genes. …”
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SF3B4 promotes Twist1 expression and clear cell renal cell carcinoma progression by facilitating the export of KLF 16 mRNA from the nucleus to the cytoplasm by Zhan Yang, Ya-Xuan Wang, Jin-Kun Wen, Hai-Tao Gao, Zhen-Wei Han, Jin-Chun Qi, Jun-Fei Gu, Chen-Ming Zhao, Hong Zhang, Bei Shi, Dan-Dan Wang, Xiao-Lu Wang, Chang-Bao Qu

“…Abstract Splicing factor 3B subunit 4 (SF3B4) plays important functional roles not only in pre-mRNA splicing, but also in the regulation of transcription, translation, and cell signaling, and its dysregulation contributes to various diseases including Nager syndrome and tumorigenesis. …”
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Molecular Targets of the 5-Amido-Carboxamide Bumped Kinase Inhibitor BKI-1748 in <i>Cryptosporidium parvum</i> and HCT-8 Host Cells by Jubilee Ajiboye, Anne-Christine Uldry, Manfred Heller, Arunasalam Naguleswaran, Erkang Fan, Wesley C. Van Voorhis, Andrew Hemphill, Joachim Müller

“…In both <i>C. parvum</i> and host cells, the largest subset of binding proteins was involved in RNA binding and modification, with a focus on ribosomal proteins and proteins involved in RNA splicing. These findings extend previous results, showing that BKI-1748 interacts with putative targets involved in common, essential pathways such as translation and RNA processing.…”
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Temporal transcriptional control of neural induction in human induced pluripotent stem cells by Shakti Gupta, Lucia Dutan Polit, Michael Fitzgerald, Helen A. Rowland, Divya Murali, Noel J. Buckley, Shankar Subramaniam, Shankar Subramaniam

“…Our temporal analysis of OTX2 target gene expression identified several OTX2 regulated gene modules representing protein remodelling, RNA splicing and RNA processing. Further CRISPRi inhibition of OTX2 prior to neural induction promotes an accelerated loss of pluripotency and a precocious and aberrant neural induction disrupting some of the previously identified modules.DiscussionWe infer that OTX2 has a diverse role during neural induction and regulates many of the biological processes that are required for loss of pluripotency and gain of neural identity. …”
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Matrin3 regulates mitotic spindle dynamics by controlling alternative splicing of CDC14B by Bruna R. Muys, Roshan L. Shrestha, Dimitrios G. Anastasakis, Lorinc Pongor, Xiao Ling Li, Ioannis Grammatikakis, Ahsan Polash, Raj Chari, Myriam Gorospe, Curtis C. Harris, Mirit I. Aladjem, Munira A. Basrai, Markus Hafner, Ashish Lal

“…Summary: Matrin3 is an RNA-binding protein that regulates diverse RNA-related processes, including mRNA splicing. Although Matrin3 has been intensively studied in neurodegenerative diseases, its function in cancer remains unclear. …”
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514 Understanding Structural and Dynamic Effects of the EWS-FLI1 interactome on the EWS Low Complexity Domain Function by George Louis Parra, Emily Selig, Antoine Baudin, Susan Weintraub, Bernard Fongang, David Libich

“…RESULTS/ANTICIPATED RESULTS: Using the proximity labeling technique TurboID, which limits the number of false-positive interactions, we have shown that EWSR1 and EWS-FLI1 act within the spliceosome (responsible for mRNA splicing and processing). Using bioinformatics techniques, we show EWS-FLI1 interacts with peptidyl-prolyl isomerase (PPI) proteins, specifically PPIL1, through the EWS-FLI1 N-terminal region (EWS-LCD). …”
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An automated framework for evaluation of deep learning models for splice site predictions by Amin Zabardast, Elif Güney Tamer, Yeşim Aydın Son, Arif Yılmaz

“…The framework eliminates time-consuming development and experimenting activities for different codebases, architectures, and configurations to obtain the best models for a given RNA splice site dataset. RNA splicing is a cellular process in which pre-mRNAs are processed into mature mRNAs and used to produce multiple mRNA transcripts from a single gene sequence. …”
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UTP11 promotes the growth of hepatocellular carcinoma by enhancing the mRNA stability of Oct4 by Yan Chen, Xiaowei Zhang, Mingcheng Zhang, Wenting Fan, Yueyue Lin, Guodong Li

“…GO-KEEG analysis from CCLE and TCGA database suggested that UTP11 might involve in RNA splicing and the stability of mRNA. Further, UTP11 was positively correlated with tumor stemness scores and stemness-associated proteins from TCGA database. …”
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Polypyrimidine tract-binding protein 3/insulin-like growth factor 2 mRNA-binding proteins 3/high-mobility group A1 axis promotes renal cancer growth and metastasis by Qianqing Wang, Fang Chen, Yu He, Yue Gao, Jiawen Wang, Sufang Chu, Pei Xie, Jiateng Zhong, Haixia Shan, Jin Bai, Pingfu Hou

“…Summary: Polypyrimidine tract-binding protein 3 (PTBP3) plays an important role in the post-transcriptional regulation of gene expression, including mRNA splicing, translation, and stability. Increasing evidence has shown that PTBP3 promotes cancer progression in several tumor types. …”
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BCAS2 regulates granulosa cell survival by participating in mRNA alternative splicing by Xiaohong Yao, Chaofan Wang, Longjie Sun, Lu Yan, Xuexue Chen, Zheng Lv, Xiaomei Xie, Shuang Tian, Wenbo liu, Lei Li, Hua Zhang, Jiali Liu

“…Conclusions Our results suggest that BCAS2 may influence the proliferation and survival of granulosa cells through regulating pre-mRNA splicing of E2f3 and Flt3l by forming the splicing complex with CDC5L and PRP19.…”
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The Heptameric SmAP1 and SmAP2 Proteins of the Crenarchaeon Sulfolobus Solfataricus Bind to Common and Distinct RNA Targets by Birgit Märtens, Gustavo Arruda Bezerra, Mathias Josef Kreuter, Irina Grishkovskaya, Andrea Manica, Valentina Arkhipova, Kristina Djinovic-Carugo, Udo Bläsi

“…Sm-based regulation is diverse and can range in scope from eukaryotic mRNA splicing to bacterial quorum sensing, with at least one step in these processes being mediated by an RNA-associated molecular assembly built on Sm proteins. …”
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A master autoantigen-ome links alternative splicing, female predilection, and COVID-19 to autoimmune diseases by Julia Y. Wang, Michael W. Roehrl, Victor B. Roehrl, Michael H. Roehrl

“…These observations and the finding that numerous autoAgs involved in RNA-splicing showed altered expression in viral infections suggest that viruses exploit alternative splicing to reprogram host cell machinery to ensure viral replication and survival. …”
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Identification of a 5-lncRNA-Based Signature for Immune Characteristics and Prognosis of Lung Squamous Cell Carcinoma and Verification of the Function of lncRNA SPATA41 by Sheng Huan, Sheng Huan, Miao Chen, Miao Chen, Sumin Sun, Yanling Zhong, Yu Chen, Yu Chen, Yihao Ji, Yihao Ji, Guoping Yin, Guoping Yin

“…GO and KEGG enrichment analysis indicated that co-expression mRNAs of the 5 lncRNAs were mainly focused on RNA splicing, DNA replication, and protein serine/threonine kinase activity. …”
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